Experiment: Varel_Bryant medium with Chlorpromazine 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1983 and BT1984 are separated by 33 nucleotides BT1984 and BT1985 overlap by 11 nucleotides BT1985 and BT1986 are separated by 199 nucleotides BT1986 and BT1987 are separated by 180 nucleotides
BT1983: BT1983 - hemolysin III (NCBI ptt file), at 2,492,878 to 2,493,567
BT1983
BT1984: BT1984 - transcriptional regulator (NCBI ptt file), at 2,493,601 to 2,493,822
BT1984
BT1985: BT1985 - hypothetical protein (NCBI ptt file), at 2,493,812 to 2,494,270
BT1985
BT1986: BT1986 - putative ribose 5-phosphate isomerase (NCBI ptt file), at 2,494,470 to 2,495,171
BT1986
BT1987: BT1987 - hypothetical protein (NCBI ptt file), at 2,495,352 to 2,497,787
BT1987
Position (kb)
2494
2495
2496 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2493.473 kb on - strand, within BT1983 at 2493.490 kb on + strand, within BT1983 at 2493.490 kb on + strand, within BT1983 at 2493.492 kb on + strand, within BT1983 at 2493.492 kb on + strand, within BT1983 at 2493.492 kb on + strand, within BT1983 at 2493.493 kb on - strand, within BT1983 at 2493.493 kb on - strand, within BT1983 at 2493.493 kb on - strand, within BT1983 at 2493.493 kb on - strand, within BT1983 at 2493.493 kb on - strand at 2493.534 kb on - strand at 2493.541 kb on - strand at 2493.560 kb on + strand at 2493.560 kb on + strand at 2493.657 kb on - strand, within BT1984 at 2493.665 kb on - strand, within BT1984 at 2493.684 kb on + strand, within BT1984 at 2493.694 kb on - strand, within BT1984 at 2493.794 kb on - strand, within BT1984 at 2493.826 kb on + strand at 2493.827 kb on - strand at 2493.827 kb on - strand at 2493.837 kb on + strand at 2493.855 kb on - strand at 2493.875 kb on + strand, within BT1985 at 2493.876 kb on - strand, within BT1985 at 2493.876 kb on - strand, within BT1985 at 2493.876 kb on - strand, within BT1985 at 2493.901 kb on + strand, within BT1985 at 2493.901 kb on + strand, within BT1985 at 2493.902 kb on - strand, within BT1985 at 2493.941 kb on + strand, within BT1985 at 2493.967 kb on - strand, within BT1985 at 2493.988 kb on + strand, within BT1985 at 2494.008 kb on - strand, within BT1985 at 2494.019 kb on + strand, within BT1985 at 2494.019 kb on + strand, within BT1985 at 2494.019 kb on + strand, within BT1985 at 2494.019 kb on + strand, within BT1985 at 2494.082 kb on + strand, within BT1985 at 2494.093 kb on - strand, within BT1985 at 2494.116 kb on - strand, within BT1985 at 2494.116 kb on - strand, within BT1985 at 2494.116 kb on - strand, within BT1985 at 2494.118 kb on - strand, within BT1985 at 2494.119 kb on + strand, within BT1985 at 2494.120 kb on - strand, within BT1985 at 2494.179 kb on - strand, within BT1985 at 2494.182 kb on - strand, within BT1985 at 2494.238 kb on - strand at 2494.259 kb on - strand at 2494.363 kb on + strand at 2494.660 kb on + strand, within BT1986 at 2494.660 kb on + strand, within BT1986 at 2494.708 kb on + strand, within BT1986 at 2494.709 kb on - strand, within BT1986 at 2494.709 kb on - strand, within BT1986 at 2494.781 kb on - strand, within BT1986 at 2494.950 kb on + strand, within BT1986 at 2494.990 kb on + strand, within BT1986 at 2495.024 kb on + strand, within BT1986 at 2495.071 kb on - strand, within BT1986 at 2495.071 kb on - strand, within BT1986 at 2495.161 kb on + strand at 2495.182 kb on + strand at 2495.182 kb on + strand at 2495.182 kb on + strand at 2495.182 kb on + strand at 2495.183 kb on - strand at 2495.225 kb on - strand at 2495.252 kb on + strand at 2495.283 kb on + strand at 2495.296 kb on - strand at 2495.309 kb on + strand at 2495.317 kb on - strand at 2495.319 kb on + strand at 2495.320 kb on - strand at 2495.348 kb on + strand at 2495.348 kb on + strand at 2495.650 kb on - strand, within BT1987 at 2495.721 kb on + strand, within BT1987 at 2495.735 kb on + strand, within BT1987 at 2495.736 kb on - strand, within BT1987 at 2495.736 kb on - strand, within BT1987 at 2495.914 kb on - strand, within BT1987 at 2496.024 kb on + strand, within BT1987 at 2496.075 kb on + strand, within BT1987 at 2496.148 kb on + strand, within BT1987 at 2496.149 kb on - strand, within BT1987
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.05 mM remove 2,493,473 - BT1983 0.86 -2.4 2,493,490 + BT1983 0.89 +2.1 2,493,490 + BT1983 0.89 -1.9 2,493,492 + BT1983 0.89 -0.5 2,493,492 + BT1983 0.89 +0.5 2,493,492 + BT1983 0.89 -1.4 2,493,493 - BT1983 0.89 -0.5 2,493,493 - BT1983 0.89 -0.4 2,493,493 - BT1983 0.89 -2.3 2,493,493 - BT1983 0.89 -1.5 2,493,493 - -1.9 2,493,534 - -1.1 2,493,541 - +0.1 2,493,560 + +0.1 2,493,560 + -0.1 2,493,657 - BT1984 0.25 -1.9 2,493,665 - BT1984 0.29 +0.1 2,493,684 + BT1984 0.37 -2.0 2,493,694 - BT1984 0.42 -1.3 2,493,794 - BT1984 0.87 -2.1 2,493,826 + -2.4 2,493,827 - -0.8 2,493,827 - -0.6 2,493,837 + -1.3 2,493,855 - +0.1 2,493,875 + BT1985 0.14 -3.2 2,493,876 - BT1985 0.14 +0.1 2,493,876 - BT1985 0.14 -0.2 2,493,876 - BT1985 0.14 -1.8 2,493,901 + BT1985 0.19 -1.6 2,493,901 + BT1985 0.19 -2.4 2,493,902 - BT1985 0.20 -0.5 2,493,941 + BT1985 0.28 -0.9 2,493,967 - BT1985 0.34 +0.1 2,493,988 + BT1985 0.38 +0.7 2,494,008 - BT1985 0.43 -1.1 2,494,019 + BT1985 0.45 -1.9 2,494,019 + BT1985 0.45 -1.8 2,494,019 + BT1985 0.45 -0.5 2,494,019 + BT1985 0.45 -1.9 2,494,082 + BT1985 0.59 +1.1 2,494,093 - BT1985 0.61 +0.1 2,494,116 - BT1985 0.66 -1.6 2,494,116 - BT1985 0.66 +3.1 2,494,116 - BT1985 0.66 -1.6 2,494,118 - BT1985 0.67 -1.5 2,494,119 + BT1985 0.67 +0.1 2,494,120 - BT1985 0.67 -2.5 2,494,179 - BT1985 0.80 -0.1 2,494,182 - BT1985 0.81 -1.6 2,494,238 - -1.8 2,494,259 - -1.4 2,494,363 + -1.2 2,494,660 + BT1986 0.27 +0.3 2,494,660 + BT1986 0.27 -2.4 2,494,708 + BT1986 0.34 -1.9 2,494,709 - BT1986 0.34 -0.5 2,494,709 - BT1986 0.34 -1.7 2,494,781 - BT1986 0.44 -1.9 2,494,950 + BT1986 0.68 -2.8 2,494,990 + BT1986 0.74 -2.4 2,495,024 + BT1986 0.79 -0.8 2,495,071 - BT1986 0.86 -0.9 2,495,071 - BT1986 0.86 -1.9 2,495,161 + -1.3 2,495,182 + +0.1 2,495,182 + -0.5 2,495,182 + -0.5 2,495,182 + +1.1 2,495,183 - -1.8 2,495,225 - -0.4 2,495,252 + -0.5 2,495,283 + +1.1 2,495,296 - -2.3 2,495,309 + -3.4 2,495,317 - +0.1 2,495,319 + -0.7 2,495,320 - +1.5 2,495,348 + -3.1 2,495,348 + +1.1 2,495,650 - BT1987 0.12 +0.4 2,495,721 + BT1987 0.15 +1.9 2,495,735 + BT1987 0.16 -2.4 2,495,736 - BT1987 0.16 +1.2 2,495,736 - BT1987 0.16 -0.8 2,495,914 - BT1987 0.23 -2.9 2,496,024 + BT1987 0.28 +4.1 2,496,075 + BT1987 0.30 -1.6 2,496,148 + BT1987 0.33 +0.1 2,496,149 - BT1987 0.33 -0.8
Or see this region's nucleotide sequence