Experiment: Varel_Bryant medium with Chlorpromazine 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1910 and BT1911 are separated by 383 nucleotides BT1911 and BT1912 are separated by 619 nucleotides BT1912 and BT1913 are separated by 109 nucleotides
BT1910: BT1910 - conserved hypothetical protein (NCBI ptt file), at 2,398,726 to 2,399,664
BT1910
BT1911: BT1911 - 7-alpha-hydroxysteroid dehydrogenase (NCBI ptt file), at 2,400,048 to 2,400,827
BT1911
BT1912: BT1912 - hypothetical protein (NCBI ptt file), at 2,401,447 to 2,401,650
BT1912
BT1913: BT1913 - nicotinate phosphoribosyltransferase (NCBI ptt file), at 2,401,760 to 2,402,929
BT1913
Position (kb)
2400
2401 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2399.151 kb on + strand, within BT1910 at 2399.151 kb on + strand, within BT1910 at 2399.152 kb on - strand, within BT1910 at 2399.232 kb on - strand, within BT1910 at 2399.232 kb on - strand, within BT1910 at 2399.313 kb on - strand, within BT1910 at 2399.313 kb on - strand, within BT1910 at 2399.316 kb on + strand, within BT1910 at 2399.370 kb on + strand, within BT1910 at 2399.371 kb on - strand, within BT1910 at 2399.371 kb on - strand, within BT1910 at 2399.472 kb on - strand, within BT1910 at 2399.507 kb on - strand, within BT1910 at 2399.531 kb on - strand, within BT1910 at 2399.547 kb on + strand, within BT1910 at 2399.547 kb on + strand, within BT1910 at 2399.554 kb on - strand, within BT1910 at 2399.610 kb on - strand at 2399.650 kb on + strand at 2399.650 kb on + strand at 2399.657 kb on + strand at 2399.697 kb on + strand at 2399.697 kb on + strand at 2399.715 kb on + strand at 2399.716 kb on - strand at 2399.757 kb on + strand at 2399.831 kb on + strand at 2399.843 kb on + strand at 2399.965 kb on + strand at 2400.004 kb on + strand at 2400.012 kb on - strand at 2400.015 kb on + strand at 2400.045 kb on + strand at 2400.046 kb on - strand at 2400.046 kb on - strand at 2400.047 kb on + strand at 2400.048 kb on - strand at 2400.048 kb on - strand at 2400.228 kb on + strand, within BT1911 at 2400.228 kb on + strand, within BT1911 at 2400.298 kb on + strand, within BT1911 at 2400.299 kb on - strand, within BT1911 at 2400.304 kb on + strand, within BT1911 at 2400.304 kb on + strand, within BT1911 at 2400.304 kb on + strand, within BT1911 at 2400.305 kb on - strand, within BT1911 at 2400.308 kb on + strand, within BT1911 at 2400.414 kb on + strand, within BT1911 at 2400.476 kb on - strand, within BT1911 at 2400.482 kb on + strand, within BT1911 at 2400.483 kb on - strand, within BT1911 at 2400.483 kb on - strand, within BT1911 at 2400.483 kb on - strand, within BT1911 at 2400.665 kb on + strand, within BT1911 at 2400.668 kb on - strand, within BT1911 at 2400.726 kb on - strand, within BT1911 at 2400.756 kb on + strand at 2400.756 kb on + strand at 2400.756 kb on + strand at 2400.825 kb on + strand at 2400.826 kb on - strand at 2400.826 kb on - strand at 2400.829 kb on + strand at 2400.829 kb on + strand at 2400.830 kb on - strand at 2400.830 kb on - strand at 2400.900 kb on - strand at 2400.915 kb on - strand at 2400.928 kb on + strand at 2400.978 kb on + strand at 2400.996 kb on - strand at 2401.023 kb on - strand at 2401.069 kb on - strand at 2401.103 kb on + strand at 2401.103 kb on + strand at 2401.103 kb on + strand at 2401.114 kb on + strand at 2401.243 kb on - strand at 2401.281 kb on + strand at 2401.341 kb on + strand at 2401.341 kb on + strand at 2401.342 kb on - strand at 2401.344 kb on + strand at 2401.345 kb on - strand at 2401.494 kb on + strand, within BT1912 at 2401.495 kb on - strand, within BT1912 at 2401.521 kb on + strand, within BT1912 at 2401.572 kb on + strand, within BT1912 at 2401.666 kb on - strand at 2401.743 kb on + strand at 2401.767 kb on + strand at 2401.768 kb on - strand at 2401.776 kb on + strand at 2401.794 kb on - strand at 2401.795 kb on + strand at 2401.795 kb on + strand at 2401.795 kb on + strand at 2401.795 kb on + strand at 2401.796 kb on - strand at 2401.796 kb on - strand at 2401.812 kb on - strand at 2401.817 kb on + strand at 2401.818 kb on - strand at 2401.818 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.05 mM remove 2,399,151 + BT1910 0.45 -2.4 2,399,151 + BT1910 0.45 -0.2 2,399,152 - BT1910 0.45 -1.8 2,399,232 - BT1910 0.54 +1.1 2,399,232 - BT1910 0.54 -1.9 2,399,313 - BT1910 0.63 +2.1 2,399,313 - BT1910 0.63 -1.4 2,399,316 + BT1910 0.63 -1.5 2,399,370 + BT1910 0.69 -1.3 2,399,371 - BT1910 0.69 +1.1 2,399,371 - BT1910 0.69 -1.9 2,399,472 - BT1910 0.79 -1.1 2,399,507 - BT1910 0.83 +1.1 2,399,531 - BT1910 0.86 -1.3 2,399,547 + BT1910 0.87 -0.5 2,399,547 + BT1910 0.87 -0.1 2,399,554 - BT1910 0.88 -1.4 2,399,610 - -2.6 2,399,650 + -1.5 2,399,650 + -0.5 2,399,657 + -0.3 2,399,697 + -4.0 2,399,697 + -0.9 2,399,715 + -0.9 2,399,716 - -1.9 2,399,757 + -0.7 2,399,831 + -0.5 2,399,843 + +1.1 2,399,965 + -1.3 2,400,004 + -2.0 2,400,012 - -0.1 2,400,015 + -0.5 2,400,045 + -1.9 2,400,046 - +1.1 2,400,046 - +0.1 2,400,047 + +2.1 2,400,048 - +1.1 2,400,048 - -1.9 2,400,228 + BT1911 0.23 +0.1 2,400,228 + BT1911 0.23 +0.7 2,400,298 + BT1911 0.32 +1.1 2,400,299 - BT1911 0.32 +0.1 2,400,304 + BT1911 0.33 -0.5 2,400,304 + BT1911 0.33 -0.6 2,400,304 + BT1911 0.33 +1.6 2,400,305 - BT1911 0.33 -0.6 2,400,308 + BT1911 0.33 -0.5 2,400,414 + BT1911 0.47 -1.5 2,400,476 - BT1911 0.55 +0.1 2,400,482 + BT1911 0.56 -0.8 2,400,483 - BT1911 0.56 +0.6 2,400,483 - BT1911 0.56 -0.5 2,400,483 - BT1911 0.56 +1.5 2,400,665 + BT1911 0.79 +1.4 2,400,668 - BT1911 0.79 -0.8 2,400,726 - BT1911 0.87 +1.4 2,400,756 + -2.5 2,400,756 + -0.9 2,400,756 + -1.5 2,400,825 + -1.5 2,400,826 - -2.4 2,400,826 - +0.6 2,400,829 + +1.1 2,400,829 + +1.1 2,400,830 - -1.0 2,400,830 - -2.6 2,400,900 - -0.7 2,400,915 - +0.3 2,400,928 + -0.9 2,400,978 + -1.3 2,400,996 - +2.6 2,401,023 - -1.9 2,401,069 - +0.1 2,401,103 + -0.8 2,401,103 + -1.2 2,401,103 + -1.2 2,401,114 + -1.5 2,401,243 - -1.1 2,401,281 + -1.8 2,401,341 + +1.1 2,401,341 + +1.9 2,401,342 - -2.3 2,401,344 + -0.3 2,401,345 - -0.5 2,401,494 + BT1912 0.23 -1.7 2,401,495 - BT1912 0.24 -1.5 2,401,521 + BT1912 0.36 -1.3 2,401,572 + BT1912 0.61 +2.1 2,401,666 - +0.5 2,401,743 + +0.1 2,401,767 + +0.1 2,401,768 - +0.2 2,401,776 + +0.6 2,401,794 - -0.7 2,401,795 + +1.6 2,401,795 + -1.4 2,401,795 + -1.5 2,401,795 + -0.9 2,401,796 - -2.3 2,401,796 - +1.1 2,401,812 - +1.1 2,401,817 + +2.6 2,401,818 - -1.8 2,401,818 - -0.5
Or see this region's nucleotide sequence