Experiment: Varel_Bryant medium with Chlorpromazine 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1368 and BT1369 are separated by 19 nucleotides BT1369 and BT1370 overlap by 4 nucleotides BT1370 and BT1371 are separated by 137 nucleotides
BT1368: BT1368 - UDP-N-acetylenolpyruvoylglucosamine reductase (NCBI ptt file), at 1,696,842 to 1,697,837
BT1368
BT1369: BT1369 - hypothetical protein (NCBI ptt file), at 1,697,857 to 1,698,705
BT1369
BT1370: BT1370 - NAD dependent nucleotide-diphosphate-sugar epimerase (NCBI ptt file), at 1,698,702 to 1,699,658
BT1370
BT1371: BT1371 - 2-amino-3-ketobutyrate coenzyme A ligase (NCBI ptt file), at 1,699,796 to 1,700,983
BT1371
Position (kb)
1698
1699
1700 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1697.859 kb on - strand at 1697.881 kb on - strand at 1697.997 kb on - strand, within BT1369 at 1698.250 kb on - strand, within BT1369 at 1698.372 kb on - strand, within BT1369 at 1698.392 kb on - strand, within BT1369 at 1698.860 kb on - strand, within BT1370 at 1699.016 kb on - strand, within BT1370 at 1699.016 kb on - strand, within BT1370 at 1699.030 kb on - strand, within BT1370 at 1699.047 kb on - strand, within BT1370 at 1699.232 kb on - strand, within BT1370 at 1699.236 kb on - strand, within BT1370 at 1699.236 kb on - strand, within BT1370 at 1699.441 kb on - strand, within BT1370 at 1699.570 kb on - strand at 1699.687 kb on - strand at 1699.703 kb on - strand at 1699.795 kb on + strand at 1699.799 kb on + strand at 1699.843 kb on + strand at 1700.017 kb on + strand, within BT1371 at 1700.018 kb on - strand, within BT1371 at 1700.055 kb on + strand, within BT1371 at 1700.055 kb on + strand, within BT1371 at 1700.056 kb on - strand, within BT1371 at 1700.059 kb on + strand, within BT1371 at 1700.060 kb on - strand, within BT1371 at 1700.060 kb on - strand, within BT1371 at 1700.060 kb on - strand, within BT1371 at 1700.356 kb on - strand, within BT1371 at 1700.362 kb on + strand, within BT1371 at 1700.394 kb on - strand, within BT1371 at 1700.407 kb on - strand, within BT1371 at 1700.407 kb on - strand, within BT1371 at 1700.471 kb on + strand, within BT1371 at 1700.498 kb on + strand, within BT1371 at 1700.499 kb on - strand, within BT1371 at 1700.500 kb on + strand, within BT1371
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.05 mM remove 1,697,859 - +0.5 1,697,881 - +0.0 1,697,997 - BT1369 0.16 -0.8 1,698,250 - BT1369 0.46 +0.5 1,698,372 - BT1369 0.61 -1.0 1,698,392 - BT1369 0.63 +1.6 1,698,860 - BT1370 0.17 -0.9 1,699,016 - BT1370 0.33 -4.0 1,699,016 - BT1370 0.33 +0.1 1,699,030 - BT1370 0.34 -1.5 1,699,047 - BT1370 0.36 +0.1 1,699,232 - BT1370 0.55 -1.3 1,699,236 - BT1370 0.56 -3.0 1,699,236 - BT1370 0.56 -1.9 1,699,441 - BT1370 0.77 -0.5 1,699,570 - -2.9 1,699,687 - -0.5 1,699,703 - -1.5 1,699,795 + -0.5 1,699,799 + -2.4 1,699,843 + -1.3 1,700,017 + BT1371 0.19 -0.5 1,700,018 - BT1371 0.19 -1.5 1,700,055 + BT1371 0.22 -1.3 1,700,055 + BT1371 0.22 -1.5 1,700,056 - BT1371 0.22 -3.8 1,700,059 + BT1371 0.22 -3.5 1,700,060 - BT1371 0.22 -0.5 1,700,060 - BT1371 0.22 -1.8 1,700,060 - BT1371 0.22 -0.5 1,700,356 - BT1371 0.47 -3.3 1,700,362 + BT1371 0.48 -1.5 1,700,394 - BT1371 0.50 -1.3 1,700,407 - BT1371 0.51 -0.5 1,700,407 - BT1371 0.51 -0.8 1,700,471 + BT1371 0.57 -2.4 1,700,498 + BT1371 0.59 -2.5 1,700,499 - BT1371 0.59 -2.3 1,700,500 + BT1371 0.59 -1.3
Or see this region's nucleotide sequence