Experiment: Varel_Bryant medium with Chlorpromazine 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1212 and BT1213 are separated by 51 nucleotides BT1213 and BT1214 are separated by 6 nucleotides BT1214 and BT1215 are separated by 89 nucleotides
BT1212: BT1212 - outer membrane efflux protein (NCBI ptt file), at 1,508,365 to 1,509,756
BT1212
BT1213: BT1213 - periplasmic component of efflux system (NCBI ptt file), at 1,509,808 to 1,511,037
BT1213
BT1214: BT1214 - putative ABC transporter ATP-binding protein (NCBI ptt file), at 1,511,044 to 1,511,787
BT1214
BT1215: BT1215 - ABC transporter, permease protein (NCBI ptt file), at 1,511,877 to 1,513,097
BT1215
Position (kb)
1509
1510
1511
1512 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1508.882 kb on - strand, within BT1212 at 1509.033 kb on - strand, within BT1212 at 1509.036 kb on - strand, within BT1212 at 1509.062 kb on - strand, within BT1212 at 1509.119 kb on - strand, within BT1212 at 1509.444 kb on - strand, within BT1212 at 1509.545 kb on - strand, within BT1212 at 1509.574 kb on - strand, within BT1212 at 1509.694 kb on + strand at 1509.694 kb on + strand at 1509.755 kb on - strand at 1509.804 kb on + strand at 1509.826 kb on - strand at 1509.983 kb on + strand, within BT1213 at 1509.984 kb on - strand, within BT1213 at 1509.984 kb on - strand, within BT1213 at 1510.008 kb on - strand, within BT1213 at 1510.126 kb on + strand, within BT1213 at 1510.127 kb on - strand, within BT1213 at 1510.299 kb on + strand, within BT1213 at 1510.321 kb on - strand, within BT1213 at 1510.439 kb on + strand, within BT1213 at 1510.474 kb on - strand, within BT1213 at 1510.619 kb on + strand, within BT1213 at 1510.933 kb on - strand at 1511.161 kb on - strand, within BT1214 at 1511.381 kb on - strand, within BT1214 at 1511.467 kb on - strand, within BT1214 at 1511.631 kb on + strand, within BT1214 at 1511.632 kb on - strand, within BT1214 at 1511.632 kb on - strand, within BT1214 at 1511.638 kb on - strand, within BT1214 at 1511.639 kb on + strand, within BT1214 at 1511.827 kb on - strand at 1511.877 kb on - strand at 1511.893 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.05 mM remove 1,508,882 - BT1212 0.37 -2.3 1,509,033 - BT1212 0.48 +0.3 1,509,036 - BT1212 0.48 -1.5 1,509,062 - BT1212 0.50 +0.1 1,509,119 - BT1212 0.54 -1.9 1,509,444 - BT1212 0.78 +0.1 1,509,545 - BT1212 0.85 -2.4 1,509,574 - BT1212 0.87 -1.4 1,509,694 + -0.5 1,509,694 + -1.3 1,509,755 - +1.1 1,509,804 + +1.1 1,509,826 - +0.7 1,509,983 + BT1213 0.14 -2.7 1,509,984 - BT1213 0.14 -2.3 1,509,984 - BT1213 0.14 -1.8 1,510,008 - BT1213 0.16 +0.1 1,510,126 + BT1213 0.26 -2.6 1,510,127 - BT1213 0.26 -0.5 1,510,299 + BT1213 0.40 -2.1 1,510,321 - BT1213 0.42 -3.3 1,510,439 + BT1213 0.51 +0.5 1,510,474 - BT1213 0.54 -1.3 1,510,619 + BT1213 0.66 -1.9 1,510,933 - +0.4 1,511,161 - BT1214 0.16 +0.2 1,511,381 - BT1214 0.45 -0.3 1,511,467 - BT1214 0.57 -0.8 1,511,631 + BT1214 0.79 -0.5 1,511,632 - BT1214 0.79 +0.1 1,511,632 - BT1214 0.79 +2.6 1,511,638 - BT1214 0.80 -1.3 1,511,639 + BT1214 0.80 +0.1 1,511,827 - +1.1 1,511,877 - +0.1 1,511,893 + -1.1
Or see this region's nucleotide sequence