Experiment: Varel_Bryant medium with Chlorpromazine 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0499 and BT0500 are separated by 48 nucleotides BT0500 and BT0501 are separated by 99 nucleotides BT0501 and BT0502 are separated by 89 nucleotides
BT0499: BT0499 - cation efflux system protein (NCBI ptt file), at 617,477 to 618,376
BT0499
BT0500: BT0500 - putative transport protein (NCBI ptt file), at 618,425 to 620,023
BT0500
BT0501: BT0501 - hypothetical protein (NCBI ptt file), at 620,123 to 620,455
BT0501
BT0502: BT0502 - TonB-dependent outer membrane receptor (NCBI ptt file), at 620,545 to 622,872
BT0502
Position (kb) 618
619
620
621 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 617.455 kb on - strand at 617.479 kb on - strand at 617.533 kb on + strand at 617.543 kb on - strand at 617.555 kb on - strand at 617.555 kb on - strand at 617.568 kb on + strand, within BT0499 at 617.663 kb on - strand, within BT0499 at 617.663 kb on - strand, within BT0499 at 617.688 kb on + strand, within BT0499 at 617.708 kb on - strand, within BT0499 at 617.717 kb on + strand, within BT0499 at 617.720 kb on - strand, within BT0499 at 617.906 kb on - strand, within BT0499 at 617.977 kb on - strand, within BT0499 at 617.993 kb on - strand, within BT0499 at 617.995 kb on + strand, within BT0499 at 617.996 kb on - strand, within BT0499 at 617.996 kb on - strand, within BT0499 at 618.005 kb on - strand, within BT0499 at 618.250 kb on + strand, within BT0499 at 618.252 kb on + strand, within BT0499 at 618.280 kb on + strand, within BT0499 at 618.281 kb on - strand, within BT0499 at 618.320 kb on - strand at 618.352 kb on + strand at 618.438 kb on + strand at 618.578 kb on - strand at 618.697 kb on + strand at 618.704 kb on - strand, within BT0500 at 618.803 kb on - strand, within BT0500 at 619.019 kb on - strand, within BT0500 at 619.082 kb on - strand, within BT0500 at 619.135 kb on - strand, within BT0500 at 619.280 kb on - strand, within BT0500 at 619.331 kb on - strand, within BT0500 at 619.528 kb on - strand, within BT0500 at 619.593 kb on + strand, within BT0500 at 619.651 kb on + strand, within BT0500 at 619.765 kb on - strand, within BT0500 at 619.797 kb on + strand, within BT0500 at 619.798 kb on - strand, within BT0500 at 619.930 kb on + strand at 619.989 kb on + strand at 620.019 kb on - strand at 620.039 kb on - strand at 620.039 kb on - strand at 620.045 kb on - strand at 620.070 kb on - strand at 620.167 kb on + strand, within BT0501 at 620.168 kb on - strand, within BT0501 at 620.381 kb on - strand, within BT0501 at 620.390 kb on + strand, within BT0501 at 620.390 kb on + strand, within BT0501 at 620.465 kb on - strand at 620.555 kb on - strand at 620.685 kb on + strand at 620.712 kb on + strand at 620.712 kb on + strand at 620.823 kb on - strand, within BT0502 at 620.849 kb on + strand, within BT0502 at 620.868 kb on + strand, within BT0502 at 620.982 kb on - strand, within BT0502
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.05 mM remove 617,455 - -0.6 617,479 - -2.0 617,533 + -0.0 617,543 - -0.2 617,555 - -1.0 617,555 - -0.0 617,568 + BT0499 0.10 -1.0 617,663 - BT0499 0.21 +0.2 617,663 - BT0499 0.21 -0.6 617,688 + BT0499 0.23 -2.4 617,708 - BT0499 0.26 -1.4 617,717 + BT0499 0.27 -1.9 617,720 - BT0499 0.27 -1.7 617,906 - BT0499 0.48 -2.1 617,977 - BT0499 0.56 -1.0 617,993 - BT0499 0.57 -1.8 617,995 + BT0499 0.58 -0.0 617,996 - BT0499 0.58 -0.0 617,996 - BT0499 0.58 -1.6 618,005 - BT0499 0.59 +1.0 618,250 + BT0499 0.86 -0.4 618,252 + BT0499 0.86 -1.0 618,280 + BT0499 0.89 +1.0 618,281 - BT0499 0.89 +1.0 618,320 - -0.8 618,352 + +0.4 618,438 + +1.0 618,578 - -0.0 618,697 + -2.0 618,704 - BT0500 0.17 -0.0 618,803 - BT0500 0.24 -2.0 619,019 - BT0500 0.37 +1.0 619,082 - BT0500 0.41 -2.8 619,135 - BT0500 0.44 -0.0 619,280 - BT0500 0.53 -0.0 619,331 - BT0500 0.57 -1.6 619,528 - BT0500 0.69 -0.0 619,593 + BT0500 0.73 -0.6 619,651 + BT0500 0.77 -1.0 619,765 - BT0500 0.84 -1.9 619,797 + BT0500 0.86 -0.6 619,798 - BT0500 0.86 -1.0 619,930 + -2.0 619,989 + +1.0 620,019 - -0.0 620,039 - +1.0 620,039 - -0.0 620,045 - +2.0 620,070 - +1.0 620,167 + BT0501 0.13 -1.6 620,168 - BT0501 0.14 -1.0 620,381 - BT0501 0.77 -1.9 620,390 + BT0501 0.80 -1.4 620,390 + BT0501 0.80 -1.0 620,465 - -1.2 620,555 - -1.3 620,685 + -0.8 620,712 + -1.0 620,712 + -0.9 620,823 - BT0502 0.12 -1.4 620,849 + BT0502 0.13 +1.0 620,868 + BT0502 0.14 -1.7 620,982 - BT0502 0.19 -0.6
Or see this region's nucleotide sequence