Experiment: Varel_Bryant medium with Chlorpromazine 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0373 and BT0374 are separated by 148 nucleotides BT0374 and BT0375 are separated by 369 nucleotides
BT0373: BT0373 - mannose-6-phosphate isomerase (NCBI ptt file), at 459,328 to 460,299
BT0373
BT0374: BT0374 - conserved hypothetical protein (NCBI ptt file), at 460,448 to 461,998
BT0374
BT0375: BT0375 - integrase (NCBI ptt file), at 462,368 to 463,057
BT0375
Position (kb)
460
461
462 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 460.312 kb on - strand at 460.320 kb on + strand at 460.408 kb on + strand at 460.408 kb on + strand at 460.430 kb on + strand at 461.551 kb on + strand, within BT0374 at 461.574 kb on - strand, within BT0374 at 461.583 kb on + strand, within BT0374 at 461.593 kb on - strand, within BT0374 at 461.620 kb on + strand, within BT0374 at 461.723 kb on + strand, within BT0374 at 461.996 kb on + strand at 462.082 kb on - strand at 462.106 kb on + strand at 462.108 kb on + strand at 462.117 kb on - strand at 462.148 kb on - strand at 462.183 kb on - strand at 462.291 kb on + strand at 462.334 kb on - strand at 462.334 kb on - strand at 462.334 kb on - strand at 462.480 kb on - strand, within BT0375 at 462.659 kb on - strand, within BT0375 at 462.774 kb on - strand, within BT0375 at 462.864 kb on - strand, within BT0375 at 462.864 kb on - strand, within BT0375 at 462.864 kb on - strand, within BT0375 at 462.865 kb on + strand, within BT0375 at 462.865 kb on + strand, within BT0375 at 462.868 kb on + strand, within BT0375 at 462.887 kb on - strand, within BT0375 at 462.889 kb on + strand, within BT0375 at 462.944 kb on - strand, within BT0375 at 462.998 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.05 mM remove 460,312 - -0.2 460,320 + +2.5 460,408 + +0.1 460,408 + -2.5 460,430 + +0.5 461,551 + BT0374 0.71 -1.6 461,574 - BT0374 0.73 -0.1 461,583 + BT0374 0.73 -1.2 461,593 - BT0374 0.74 -3.2 461,620 + BT0374 0.76 -0.6 461,723 + BT0374 0.82 -0.1 461,996 + -0.0 462,082 - -1.2 462,106 + +0.1 462,108 + -1.0 462,117 - -0.7 462,148 - +1.0 462,183 - -1.8 462,291 + -1.2 462,334 - -0.3 462,334 - -1.0 462,334 - -0.3 462,480 - BT0375 0.16 -1.0 462,659 - BT0375 0.42 +0.2 462,774 - BT0375 0.59 -2.5 462,864 - BT0375 0.72 -1.9 462,864 - BT0375 0.72 -0.6 462,864 - BT0375 0.72 -1.4 462,865 + BT0375 0.72 -1.4 462,865 + BT0375 0.72 -1.9 462,868 + BT0375 0.72 +1.0 462,887 - BT0375 0.75 -2.2 462,889 + BT0375 0.76 +1.0 462,944 - BT0375 0.83 -0.0 462,998 - -1.4
Or see this region's nucleotide sequence