Experiment: Varel_Bryant medium with Chlorpromazine 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0200 and BT0201 are separated by 9 nucleotides BT0201 and BT0202 overlap by 4 nucleotides
BT0200: BT0200 - ATP phosphoribosyltransferase (NCBI ptt file), at 215,094 to 215,945
BT0200
BT0201: BT0201 - histidinol dehydrogenase (NCBI ptt file), at 215,955 to 217,241
BT0201
BT0202: BT0202 - histidinol-phosphate aminotransferase (EC 2.6.1.9) <small>(from data)</small>, at 217,238 to 218,278
BT0202
Position (kb) 215
216
217
218 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 214.988 kb on + strand at 214.998 kb on - strand at 215.004 kb on - strand at 215.190 kb on - strand, within BT0200 at 215.230 kb on + strand, within BT0200 at 215.495 kb on - strand, within BT0200 at 215.495 kb on - strand, within BT0200 at 215.495 kb on - strand, within BT0200 at 215.577 kb on - strand, within BT0200 at 215.612 kb on - strand, within BT0200 at 215.674 kb on + strand, within BT0200 at 215.756 kb on + strand, within BT0200 at 215.771 kb on - strand, within BT0200 at 215.802 kb on - strand, within BT0200 at 215.805 kb on - strand, within BT0200 at 215.910 kb on - strand at 215.943 kb on + strand at 216.058 kb on + strand at 216.059 kb on - strand at 216.158 kb on - strand, within BT0201 at 216.204 kb on - strand, within BT0201 at 216.309 kb on - strand, within BT0201 at 216.330 kb on - strand, within BT0201 at 216.455 kb on + strand, within BT0201 at 216.455 kb on - strand, within BT0201 at 216.494 kb on - strand, within BT0201 at 216.494 kb on - strand, within BT0201 at 216.494 kb on - strand, within BT0201 at 216.534 kb on + strand, within BT0201 at 216.535 kb on - strand, within BT0201 at 216.629 kb on - strand, within BT0201 at 216.713 kb on - strand, within BT0201 at 216.787 kb on + strand, within BT0201 at 216.799 kb on + strand, within BT0201 at 216.800 kb on - strand, within BT0201 at 217.046 kb on + strand, within BT0201 at 217.047 kb on - strand, within BT0201 at 217.047 kb on - strand, within BT0201 at 217.230 kb on - strand at 217.271 kb on - strand at 217.282 kb on - strand at 217.311 kb on - strand at 217.313 kb on - strand at 217.361 kb on - strand, within BT0202 at 217.447 kb on + strand, within BT0202 at 217.448 kb on - strand, within BT0202 at 217.524 kb on + strand, within BT0202 at 217.524 kb on + strand, within BT0202 at 217.524 kb on + strand, within BT0202 at 217.525 kb on - strand, within BT0202 at 217.545 kb on - strand, within BT0202 at 217.554 kb on - strand, within BT0202 at 217.658 kb on - strand, within BT0202 at 217.731 kb on + strand, within BT0202 at 217.773 kb on - strand, within BT0202 at 217.775 kb on - strand, within BT0202 at 217.941 kb on - strand, within BT0202 at 217.941 kb on - strand, within BT0202 at 217.941 kb on - strand, within BT0202 at 217.992 kb on - strand, within BT0202 at 217.995 kb on - strand, within BT0202 at 218.084 kb on + strand, within BT0202 at 218.085 kb on - strand, within BT0202
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.05 mM remove 214,988 + +0.0 214,998 - -2.3 215,004 - +0.0 215,190 - BT0200 0.11 -2.8 215,230 + BT0200 0.16 -2.0 215,495 - BT0200 0.47 -3.8 215,495 - BT0200 0.47 +1.0 215,495 - BT0200 0.47 -2.8 215,577 - BT0200 0.57 -1.0 215,612 - BT0200 0.61 +1.0 215,674 + BT0200 0.68 -0.6 215,756 + BT0200 0.78 -3.2 215,771 - BT0200 0.79 -1.0 215,802 - BT0200 0.83 -3.5 215,805 - BT0200 0.83 +1.0 215,910 - +0.0 215,943 + -1.8 216,058 + -2.0 216,059 - +0.0 216,158 - BT0201 0.16 +0.0 216,204 - BT0201 0.19 -1.3 216,309 - BT0201 0.28 -1.8 216,330 - BT0201 0.29 -3.5 216,455 + BT0201 0.39 -3.7 216,455 - BT0201 0.39 +1.0 216,494 - BT0201 0.42 -3.8 216,494 - BT0201 0.42 +1.0 216,494 - BT0201 0.42 +1.0 216,534 + BT0201 0.45 -1.6 216,535 - BT0201 0.45 -2.2 216,629 - BT0201 0.52 -2.3 216,713 - BT0201 0.59 -0.6 216,787 + BT0201 0.65 -2.2 216,799 + BT0201 0.66 -2.2 216,800 - BT0201 0.66 -2.5 217,046 + BT0201 0.85 -1.8 217,047 - BT0201 0.85 -1.0 217,047 - BT0201 0.85 -2.5 217,230 - -0.6 217,271 - -1.0 217,282 - -2.2 217,311 - -3.5 217,313 - -1.0 217,361 - BT0202 0.12 -0.6 217,447 + BT0202 0.20 -1.6 217,448 - BT0202 0.20 -1.6 217,524 + BT0202 0.27 -1.0 217,524 + BT0202 0.27 -0.6 217,524 + BT0202 0.27 -4.1 217,525 - BT0202 0.28 -2.0 217,545 - BT0202 0.29 -0.6 217,554 - BT0202 0.30 -4.9 217,658 - BT0202 0.40 -3.7 217,731 + BT0202 0.47 +1.0 217,773 - BT0202 0.51 -1.8 217,775 - BT0202 0.52 -2.8 217,941 - BT0202 0.68 -1.0 217,941 - BT0202 0.68 -3.2 217,941 - BT0202 0.68 -2.3 217,992 - BT0202 0.72 -4.1 217,995 - BT0202 0.73 -0.6 218,084 + BT0202 0.81 -3.2 218,085 - BT0202 0.81 -3.1
Or see this region's nucleotide sequence