Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT0154

Experiment: Varel_Bryant medium with Chlorpromazine 0.05 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT0153 and BT0154 are separated by 6 nucleotidesBT0154 and BT0155 are separated by 329 nucleotides BT0153: BT0153 - hypothetical protein (NCBI ptt file), at 157,983 to 158,858 BT0153 BT0154: BT0154 - putative periplasmic protease (NCBI ptt file), at 158,865 to 160,319 BT0154 BT0155: BT0155 - putative tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (NCBI ptt file), at 160,649 to 161,716 BT0155 Position (kb) 158 159 160 161Strain fitness (log2 ratio) -2 -1 0 1 2 3at 157.921 kb on - strandat 157.921 kb on - strandat 158.024 kb on + strandat 158.037 kb on - strandat 158.037 kb on - strandat 158.042 kb on - strandat 158.045 kb on + strandat 158.096 kb on + strand, within BT0153at 158.096 kb on + strand, within BT0153at 158.097 kb on - strand, within BT0153at 158.218 kb on + strand, within BT0153at 158.219 kb on - strand, within BT0153at 158.222 kb on + strand, within BT0153at 158.279 kb on - strand, within BT0153at 158.455 kb on + strand, within BT0153at 158.495 kb on - strand, within BT0153at 158.568 kb on - strand, within BT0153at 158.653 kb on + strand, within BT0153at 158.657 kb on + strand, within BT0153at 158.657 kb on + strand, within BT0153at 158.824 kb on - strandat 158.827 kb on - strandat 158.930 kb on + strandat 158.930 kb on + strandat 159.009 kb on + strandat 159.010 kb on - strandat 159.015 kb on + strand, within BT0154at 159.154 kb on + strand, within BT0154at 159.166 kb on - strand, within BT0154at 159.215 kb on + strand, within BT0154at 159.283 kb on - strand, within BT0154at 159.436 kb on + strand, within BT0154at 159.437 kb on - strand, within BT0154at 159.579 kb on - strand, within BT0154at 159.656 kb on + strand, within BT0154at 159.689 kb on + strand, within BT0154at 159.689 kb on + strand, within BT0154at 159.759 kb on - strand, within BT0154at 159.850 kb on - strand, within BT0154at 159.854 kb on + strand, within BT0154at 159.855 kb on - strand, within BT0154at 159.878 kb on - strand, within BT0154at 159.878 kb on - strand, within BT0154at 159.892 kb on + strand, within BT0154at 159.962 kb on + strand, within BT0154at 159.967 kb on + strand, within BT0154at 159.967 kb on + strand, within BT0154at 160.066 kb on - strand, within BT0154at 160.076 kb on - strand, within BT0154at 160.154 kb on + strand, within BT0154at 160.155 kb on - strand, within BT0154at 160.156 kb on + strand, within BT0154at 160.192 kb on + strandat 160.290 kb on + strandat 160.292 kb on + strandat 160.293 kb on - strandat 160.297 kb on - strandat 160.323 kb on - strandat 160.363 kb on + strandat 160.384 kb on - strandat 160.405 kb on + strandat 160.406 kb on - strandat 160.456 kb on - strandat 160.465 kb on + strandat 160.466 kb on - strandat 160.507 kb on + strandat 160.508 kb on - strandat 160.508 kb on - strandat 160.512 kb on + strandat 160.513 kb on - strandat 160.772 kb on - strand, within BT0155at 160.823 kb on - strand, within BT0155at 160.859 kb on - strand, within BT0155at 160.865 kb on + strand, within BT0155at 160.897 kb on + strand, within BT0155at 160.997 kb on - strand, within BT0155at 161.105 kb on + strand, within BT0155at 161.105 kb on + strand, within BT0155at 161.160 kb on + strand, within BT0155at 161.160 kb on + strand, within BT0155at 161.243 kb on + strand, within BT0155at 161.292 kb on + strand, within BT0155

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Per-strain Table

Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.05 mM
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157,921 - +0.3
157,921 - -2.2
158,024 + -2.2
158,037 - -1.2
158,037 - +1.0
158,042 - +1.0
158,045 + -2.5
158,096 + BT0153 0.13 -1.0
158,096 + BT0153 0.13 +0.0
158,097 - BT0153 0.13 +0.0
158,218 + BT0153 0.27 +0.4
158,219 - BT0153 0.27 +0.4
158,222 + BT0153 0.27 +0.0
158,279 - BT0153 0.34 +1.0
158,455 + BT0153 0.54 +1.0
158,495 - BT0153 0.58 +1.6
158,568 - BT0153 0.67 -0.2
158,653 + BT0153 0.76 +0.0
158,657 + BT0153 0.77 +1.0
158,657 + BT0153 0.77 -1.0
158,824 - -0.8
158,827 - +0.3
158,930 + -2.0
158,930 + +0.4
159,009 + +1.0
159,010 - -0.6
159,015 + BT0154 0.10 +0.6
159,154 + BT0154 0.20 +2.6
159,166 - BT0154 0.21 +1.3
159,215 + BT0154 0.24 -0.8
159,283 - BT0154 0.29 +0.8
159,436 + BT0154 0.39 +2.6
159,437 - BT0154 0.39 +1.0
159,579 - BT0154 0.49 +2.3
159,656 + BT0154 0.54 +1.9
159,689 + BT0154 0.57 -2.4
159,689 + BT0154 0.57 +0.8
159,759 - BT0154 0.61 +1.0
159,850 - BT0154 0.68 -0.9
159,854 + BT0154 0.68 +1.0
159,855 - BT0154 0.68 +2.6
159,878 - BT0154 0.70 +2.2
159,878 - BT0154 0.70 +0.0
159,892 + BT0154 0.71 +2.8
159,962 + BT0154 0.75 -1.8
159,967 + BT0154 0.76 -1.6
159,967 + BT0154 0.76 +0.3
160,066 - BT0154 0.83 -1.3
160,076 - BT0154 0.83 +2.0
160,154 + BT0154 0.89 +1.0
160,155 - BT0154 0.89 +1.0
160,156 + BT0154 0.89 -0.6
160,192 + +2.0
160,290 + +2.3
160,292 + -1.2
160,293 - +0.8
160,297 - +1.0
160,323 - +1.0
160,363 + +0.5
160,384 - -1.6
160,405 + +0.0
160,406 - +0.1
160,456 - -1.6
160,465 + +2.0
160,466 - -2.3
160,507 + -0.8
160,508 - -0.6
160,508 - +3.0
160,512 + -2.6
160,513 - +0.0
160,772 - BT0155 0.12 -0.6
160,823 - BT0155 0.16 -1.0
160,859 - BT0155 0.20 -2.6
160,865 + BT0155 0.20 -1.7
160,897 + BT0155 0.23 +0.3
160,997 - BT0155 0.33 -0.6
161,105 + BT0155 0.43 -1.9
161,105 + BT0155 0.43 +1.6
161,160 + BT0155 0.48 -0.2
161,160 + BT0155 0.48 -0.5
161,243 + BT0155 0.56 +2.3
161,292 + BT0155 0.60 -1.4

Or see this region's nucleotide sequence