Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrD and tRNA-Ala are separated by 20 nucleotides tRNA-Ala and Synpcc7942_0291 are separated by 79 nucleotides Synpcc7942_0291 and dfp are separated by 3 nucleotides
Synpcc7942_0290: pyrD - dihydroorotate dehydrogenase 2, at 287,003 to 288,139
pyrD
Synpcc7942_R0030: tRNA-Ala - tRNA-Ala, at 288,160 to 288,232
tRNA-Ala
Synpcc7942_0291: Synpcc7942_0291 - hypothetical protein, at 288,312 to 288,530
_0291
Synpcc7942_0292: dfp - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase, at 288,534 to 289,760
dfp
Position (kb) 288
289 Strain fitness (log2 ratio) -2
-1
0
1
2 at 288.259 kb on - strand at 288.274 kb on + strand at 288.314 kb on + strand at 288.400 kb on - strand, within Synpcc7942_0291 at 288.437 kb on - strand, within Synpcc7942_0291 at 288.447 kb on - strand, within Synpcc7942_0291 at 288.482 kb on - strand, within Synpcc7942_0291 at 288.513 kb on - strand at 288.513 kb on - strand at 288.532 kb on - strand at 288.532 kb on - strand at 288.532 kb on - strand at 288.536 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 288,259 - +0.2 288,274 + +1.8 288,314 + -0.0 288,400 - Synpcc7942_0291 0.40 -2.2 288,437 - Synpcc7942_0291 0.57 +1.2 288,447 - Synpcc7942_0291 0.62 -1.6 288,482 - Synpcc7942_0291 0.78 -0.5 288,513 - -0.1 288,513 - -0.2 288,532 - +0.5 288,532 - -0.4 288,532 - -2.1 288,536 + -1.7
Or see this region's nucleotide sequence