Experiment: Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5918 and Pf6N2E2_5919 are separated by 22 nucleotides Pf6N2E2_5919 and Pf6N2E2_5920 overlap by 4 nucleotides Pf6N2E2_5920 and Pf6N2E2_5921 are separated by 478 nucleotides
Pf6N2E2_5918 - hypothetical protein, at 6,737,805 to 6,738,074
_5918
Pf6N2E2_5919 - Flp pilus assembly protein TadD, contains TPR repeats, at 6,738,097 to 6,738,870
_5919
Pf6N2E2_5920 - Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly, at 6,738,867 to 6,740,090
_5920
Pf6N2E2_5921 - Metal-dependent hydrolase related to alanyl-tRNA synthetase, at 6,740,569 to 6,741,195
_5921
Position (kb)
6738
6739
6740
6741 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6738.091 kb on - strand at 6738.098 kb on + strand at 6738.098 kb on + strand at 6738.098 kb on + strand at 6738.098 kb on + strand at 6738.098 kb on + strand at 6738.099 kb on - strand at 6738.099 kb on - strand at 6738.276 kb on - strand, within Pf6N2E2_5919 at 6738.384 kb on - strand, within Pf6N2E2_5919 at 6738.449 kb on + strand, within Pf6N2E2_5919 at 6738.653 kb on + strand, within Pf6N2E2_5919 at 6738.654 kb on - strand, within Pf6N2E2_5919 at 6738.815 kb on + strand at 6738.816 kb on - strand at 6738.916 kb on + strand at 6738.916 kb on + strand at 6738.916 kb on + strand at 6738.916 kb on + strand at 6738.916 kb on + strand at 6738.917 kb on - strand at 6738.917 kb on - strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.940 kb on + strand at 6738.941 kb on - strand at 6739.343 kb on - strand, within Pf6N2E2_5920 at 6739.457 kb on + strand, within Pf6N2E2_5920 at 6739.459 kb on + strand, within Pf6N2E2_5920 at 6739.505 kb on + strand, within Pf6N2E2_5920 at 6739.506 kb on - strand, within Pf6N2E2_5920 at 6739.722 kb on + strand, within Pf6N2E2_5920 at 6739.723 kb on - strand, within Pf6N2E2_5920 at 6739.789 kb on - strand, within Pf6N2E2_5920 at 6740.064 kb on + strand at 6740.064 kb on + strand at 6740.078 kb on + strand at 6740.078 kb on + strand at 6740.078 kb on + strand at 6740.079 kb on - strand at 6740.079 kb on - strand at 6740.079 kb on - strand at 6740.120 kb on + strand at 6740.121 kb on - strand at 6740.130 kb on + strand at 6740.130 kb on + strand at 6740.131 kb on - strand at 6740.131 kb on - strand at 6740.131 kb on - strand at 6740.148 kb on + strand at 6740.148 kb on + strand at 6740.148 kb on + strand at 6740.148 kb on + strand at 6740.148 kb on + strand at 6740.149 kb on - strand at 6740.149 kb on - strand at 6740.381 kb on + strand at 6740.382 kb on - strand at 6740.426 kb on + strand at 6740.582 kb on + strand at 6740.583 kb on - strand at 6740.583 kb on - strand at 6740.596 kb on + strand at 6740.596 kb on + strand at 6740.597 kb on - strand at 6740.597 kb on - strand at 6740.597 kb on - strand at 6740.679 kb on - strand, within Pf6N2E2_5921 at 6740.825 kb on - strand, within Pf6N2E2_5921 at 6740.825 kb on - strand, within Pf6N2E2_5921 at 6740.990 kb on + strand, within Pf6N2E2_5921 at 6740.990 kb on + strand, within Pf6N2E2_5921 at 6740.990 kb on + strand, within Pf6N2E2_5921 at 6740.991 kb on - strand, within Pf6N2E2_5921 at 6740.991 kb on - strand, within Pf6N2E2_5921 at 6741.025 kb on + strand, within Pf6N2E2_5921 at 6741.025 kb on + strand, within Pf6N2E2_5921 at 6741.025 kb on + strand, within Pf6N2E2_5921
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 500 mM remove 6,738,091 - -0.3 6,738,098 + +0.3 6,738,098 + +0.6 6,738,098 + +0.0 6,738,098 + -0.2 6,738,098 + -0.1 6,738,099 - +1.5 6,738,099 - -0.0 6,738,276 - Pf6N2E2_5919 0.23 +1.3 6,738,384 - Pf6N2E2_5919 0.37 -0.1 6,738,449 + Pf6N2E2_5919 0.45 +0.5 6,738,653 + Pf6N2E2_5919 0.72 -1.2 6,738,654 - Pf6N2E2_5919 0.72 +0.5 6,738,815 + +1.0 6,738,816 - +0.3 6,738,916 + +1.1 6,738,916 + -0.2 6,738,916 + +0.0 6,738,916 + +1.2 6,738,916 + +0.5 6,738,917 - +1.1 6,738,917 - +0.4 6,738,920 + +0.2 6,738,920 + +0.4 6,738,920 + +0.7 6,738,920 + -0.7 6,738,920 + -0.5 6,738,920 + -0.5 6,738,920 + +1.3 6,738,920 + -0.2 6,738,920 + -0.1 6,738,921 - -0.3 6,738,921 - -0.4 6,738,921 - +0.4 6,738,921 - +0.3 6,738,921 - +0.8 6,738,921 - +0.5 6,738,921 - +0.6 6,738,921 - -0.0 6,738,921 - +0.4 6,738,921 - -0.4 6,738,940 + +0.4 6,738,941 - -2.1 6,739,343 - Pf6N2E2_5920 0.39 +0.7 6,739,457 + Pf6N2E2_5920 0.48 +0.2 6,739,459 + Pf6N2E2_5920 0.48 +0.8 6,739,505 + Pf6N2E2_5920 0.52 +2.1 6,739,506 - Pf6N2E2_5920 0.52 +2.1 6,739,722 + Pf6N2E2_5920 0.70 +0.7 6,739,723 - Pf6N2E2_5920 0.70 +0.0 6,739,789 - Pf6N2E2_5920 0.75 -0.3 6,740,064 + +0.1 6,740,064 + -1.2 6,740,078 + +0.8 6,740,078 + +0.5 6,740,078 + +0.3 6,740,079 - -0.8 6,740,079 - -0.1 6,740,079 - -0.8 6,740,120 + -0.0 6,740,121 - -0.0 6,740,130 + +0.6 6,740,130 + -0.4 6,740,131 - -1.8 6,740,131 - -0.4 6,740,131 - +1.1 6,740,148 + -0.1 6,740,148 + -1.2 6,740,148 + +1.1 6,740,148 + +0.2 6,740,148 + -0.6 6,740,149 - +0.3 6,740,149 - -1.7 6,740,381 + +0.5 6,740,382 - +1.5 6,740,426 + +0.8 6,740,582 + -1.4 6,740,583 - -0.6 6,740,583 - +0.2 6,740,596 + -0.8 6,740,596 + -0.9 6,740,597 - -0.3 6,740,597 - -0.3 6,740,597 - +0.3 6,740,679 - Pf6N2E2_5921 0.18 +0.0 6,740,825 - Pf6N2E2_5921 0.41 -0.3 6,740,825 - Pf6N2E2_5921 0.41 +0.3 6,740,990 + Pf6N2E2_5921 0.67 +0.2 6,740,990 + Pf6N2E2_5921 0.67 +0.2 6,740,990 + Pf6N2E2_5921 0.67 +0.5 6,740,991 - Pf6N2E2_5921 0.67 -2.4 6,740,991 - Pf6N2E2_5921 0.67 +0.5 6,741,025 + Pf6N2E2_5921 0.73 -0.1 6,741,025 + Pf6N2E2_5921 0.73 -0.9 6,741,025 + Pf6N2E2_5921 0.73 -1.5
Or see this region's nucleotide sequence