Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_544

Experiment: Chloride 500 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_543 and Pf6N2E2_544 are separated by 153 nucleotidesPf6N2E2_544 and Pf6N2E2_545 are separated by 24 nucleotides Pf6N2E2_543 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 612,214 to 613,329 _543 Pf6N2E2_544 - Short-chain dehydrogenase/reductase SDR, at 613,483 to 614,250 _544 Pf6N2E2_545 - Predicted aminoglycoside phosphotransferase, at 614,275 to 615,342 _545 Position (kb) 613 614 615Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 612.648 kb on + strand, within Pf6N2E2_543at 612.648 kb on + strand, within Pf6N2E2_543at 612.648 kb on + strand, within Pf6N2E2_543at 612.648 kb on + strand, within Pf6N2E2_543at 612.648 kb on + strand, within Pf6N2E2_543at 612.648 kb on + strand, within Pf6N2E2_543at 612.648 kb on + strand, within Pf6N2E2_543at 612.649 kb on - strand, within Pf6N2E2_543at 612.649 kb on - strand, within Pf6N2E2_543at 612.649 kb on - strand, within Pf6N2E2_543at 612.935 kb on + strand, within Pf6N2E2_543at 612.935 kb on + strand, within Pf6N2E2_543at 612.936 kb on - strand, within Pf6N2E2_543at 612.936 kb on - strand, within Pf6N2E2_543at 612.936 kb on - strand, within Pf6N2E2_543at 612.941 kb on + strand, within Pf6N2E2_543at 612.941 kb on + strand, within Pf6N2E2_543at 612.942 kb on - strand, within Pf6N2E2_543at 613.031 kb on + strand, within Pf6N2E2_543at 613.032 kb on - strand, within Pf6N2E2_543at 613.444 kb on + strandat 613.444 kb on + strandat 613.445 kb on - strandat 613.445 kb on - strandat 613.445 kb on - strandat 613.445 kb on - strandat 613.445 kb on - strandat 613.445 kb on - strandat 613.529 kb on + strandat 613.529 kb on + strandat 613.553 kb on + strandat 613.553 kb on + strandat 613.554 kb on - strandat 613.769 kb on + strand, within Pf6N2E2_544at 613.769 kb on + strand, within Pf6N2E2_544at 613.769 kb on + strand, within Pf6N2E2_544at 613.770 kb on - strand, within Pf6N2E2_544at 613.773 kb on + strand, within Pf6N2E2_544at 613.774 kb on - strand, within Pf6N2E2_544at 614.266 kb on + strandat 614.266 kb on + strandat 614.266 kb on + strandat 614.266 kb on + strandat 614.266 kb on + strandat 614.266 kb on + strandat 614.266 kb on + strandat 614.267 kb on - strandat 614.267 kb on - strandat 614.267 kb on - strandat 614.267 kb on - strandat 614.267 kb on - strandat 614.387 kb on + strand, within Pf6N2E2_545at 614.387 kb on + strand, within Pf6N2E2_545at 614.387 kb on + strand, within Pf6N2E2_545at 614.388 kb on - strand, within Pf6N2E2_545at 614.388 kb on - strand, within Pf6N2E2_545at 614.388 kb on - strand, within Pf6N2E2_545at 614.388 kb on - strand, within Pf6N2E2_545at 614.426 kb on + strand, within Pf6N2E2_545at 614.426 kb on + strand, within Pf6N2E2_545at 614.426 kb on + strand, within Pf6N2E2_545at 614.426 kb on + strand, within Pf6N2E2_545at 614.427 kb on - strand, within Pf6N2E2_545at 614.427 kb on - strand, within Pf6N2E2_545at 614.432 kb on + strand, within Pf6N2E2_545at 614.432 kb on + strand, within Pf6N2E2_545at 614.432 kb on + strand, within Pf6N2E2_545at 614.432 kb on + strand, within Pf6N2E2_545at 614.433 kb on - strand, within Pf6N2E2_545at 614.433 kb on - strand, within Pf6N2E2_545at 614.433 kb on - strand, within Pf6N2E2_545at 614.435 kb on + strand, within Pf6N2E2_545at 614.435 kb on + strand, within Pf6N2E2_545at 614.435 kb on + strand, within Pf6N2E2_545at 614.435 kb on + strand, within Pf6N2E2_545at 614.435 kb on + strand, within Pf6N2E2_545at 614.435 kb on + strand, within Pf6N2E2_545at 614.435 kb on + strand, within Pf6N2E2_545at 614.435 kb on + strand, within Pf6N2E2_545at 614.435 kb on + strand, within Pf6N2E2_545at 614.436 kb on - strand, within Pf6N2E2_545at 614.436 kb on - strand, within Pf6N2E2_545at 614.436 kb on - strand, within Pf6N2E2_545at 614.436 kb on - strand, within Pf6N2E2_545at 614.436 kb on - strand, within Pf6N2E2_545at 614.436 kb on - strand, within Pf6N2E2_545at 614.436 kb on - strand, within Pf6N2E2_545at 614.477 kb on + strand, within Pf6N2E2_545at 614.478 kb on - strand, within Pf6N2E2_545at 614.481 kb on - strand, within Pf6N2E2_545at 614.693 kb on + strand, within Pf6N2E2_545at 615.011 kb on + strand, within Pf6N2E2_545at 615.011 kb on + strand, within Pf6N2E2_545at 615.012 kb on - strand, within Pf6N2E2_545at 615.012 kb on - strand, within Pf6N2E2_545at 615.012 kb on - strand, within Pf6N2E2_545at 615.053 kb on + strand, within Pf6N2E2_545at 615.053 kb on + strand, within Pf6N2E2_545at 615.194 kb on + strand, within Pf6N2E2_545at 615.194 kb on + strand, within Pf6N2E2_545at 615.195 kb on - strand, within Pf6N2E2_545at 615.195 kb on - strand, within Pf6N2E2_545

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Per-strain Table

Position Strand Gene LocusTag Fraction Chloride 500 mM
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612,648 + Pf6N2E2_543 0.39 +0.5
612,648 + Pf6N2E2_543 0.39 -0.2
612,648 + Pf6N2E2_543 0.39 -0.8
612,648 + Pf6N2E2_543 0.39 +0.4
612,648 + Pf6N2E2_543 0.39 -0.9
612,648 + Pf6N2E2_543 0.39 -0.8
612,648 + Pf6N2E2_543 0.39 +0.0
612,649 - Pf6N2E2_543 0.39 -0.2
612,649 - Pf6N2E2_543 0.39 -0.5
612,649 - Pf6N2E2_543 0.39 +0.2
612,935 + Pf6N2E2_543 0.65 -1.2
612,935 + Pf6N2E2_543 0.65 +0.6
612,936 - Pf6N2E2_543 0.65 +1.3
612,936 - Pf6N2E2_543 0.65 +0.5
612,936 - Pf6N2E2_543 0.65 +0.9
612,941 + Pf6N2E2_543 0.65 -0.5
612,941 + Pf6N2E2_543 0.65 -1.2
612,942 - Pf6N2E2_543 0.65 +0.2
613,031 + Pf6N2E2_543 0.73 -0.4
613,032 - Pf6N2E2_543 0.73 +0.2
613,444 + -1.1
613,444 + +0.8
613,445 - +0.3
613,445 - +0.1
613,445 - +0.0
613,445 - +1.1
613,445 - -0.7
613,445 - -0.6
613,529 + +0.0
613,529 + -0.5
613,553 + -0.5
613,553 + -0.5
613,554 - -1.2
613,769 + Pf6N2E2_544 0.37 +0.0
613,769 + Pf6N2E2_544 0.37 -0.8
613,769 + Pf6N2E2_544 0.37 -1.9
613,770 - Pf6N2E2_544 0.37 -0.1
613,773 + Pf6N2E2_544 0.38 -3.7
613,774 - Pf6N2E2_544 0.38 -0.2
614,266 + -0.2
614,266 + -0.1
614,266 + +0.1
614,266 + +0.8
614,266 + +0.9
614,266 + +0.6
614,266 + -1.9
614,267 - +0.9
614,267 - +0.5
614,267 - -0.6
614,267 - +0.0
614,267 - -0.8
614,387 + Pf6N2E2_545 0.10 +0.8
614,387 + Pf6N2E2_545 0.10 +2.0
614,387 + Pf6N2E2_545 0.10 +0.1
614,388 - Pf6N2E2_545 0.11 -1.8
614,388 - Pf6N2E2_545 0.11 +0.5
614,388 - Pf6N2E2_545 0.11 +0.9
614,388 - Pf6N2E2_545 0.11 +1.3
614,426 + Pf6N2E2_545 0.14 +0.3
614,426 + Pf6N2E2_545 0.14 -0.2
614,426 + Pf6N2E2_545 0.14 -0.7
614,426 + Pf6N2E2_545 0.14 -0.7
614,427 - Pf6N2E2_545 0.14 +1.5
614,427 - Pf6N2E2_545 0.14 +1.3
614,432 + Pf6N2E2_545 0.15 -1.5
614,432 + Pf6N2E2_545 0.15 -1.5
614,432 + Pf6N2E2_545 0.15 -1.0
614,432 + Pf6N2E2_545 0.15 +0.2
614,433 - Pf6N2E2_545 0.15 -0.7
614,433 - Pf6N2E2_545 0.15 +1.3
614,433 - Pf6N2E2_545 0.15 -0.3
614,435 + Pf6N2E2_545 0.15 +1.7
614,435 + Pf6N2E2_545 0.15 +0.3
614,435 + Pf6N2E2_545 0.15 -0.4
614,435 + Pf6N2E2_545 0.15 +0.5
614,435 + Pf6N2E2_545 0.15 +0.8
614,435 + Pf6N2E2_545 0.15 -0.1
614,435 + Pf6N2E2_545 0.15 +0.7
614,435 + Pf6N2E2_545 0.15 -0.7
614,435 + Pf6N2E2_545 0.15 -1.8
614,436 - Pf6N2E2_545 0.15 +2.5
614,436 - Pf6N2E2_545 0.15 -0.2
614,436 - Pf6N2E2_545 0.15 -1.2
614,436 - Pf6N2E2_545 0.15 -0.8
614,436 - Pf6N2E2_545 0.15 -1.2
614,436 - Pf6N2E2_545 0.15 -0.1
614,436 - Pf6N2E2_545 0.15 +0.7
614,477 + Pf6N2E2_545 0.19 +0.5
614,478 - Pf6N2E2_545 0.19 -0.9
614,481 - Pf6N2E2_545 0.19 -0.7
614,693 + Pf6N2E2_545 0.39 -0.3
615,011 + Pf6N2E2_545 0.69 -0.5
615,011 + Pf6N2E2_545 0.69 +0.2
615,012 - Pf6N2E2_545 0.69 -0.4
615,012 - Pf6N2E2_545 0.69 -0.8
615,012 - Pf6N2E2_545 0.69 +2.0
615,053 + Pf6N2E2_545 0.73 +0.5
615,053 + Pf6N2E2_545 0.73 -0.6
615,194 + Pf6N2E2_545 0.86 -0.5
615,194 + Pf6N2E2_545 0.86 -1.1
615,195 - Pf6N2E2_545 0.86 -0.1
615,195 - Pf6N2E2_545 0.86 -1.4

Or see this region's nucleotide sequence