Experiment: Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4677 and Pf6N2E2_4678 overlap by 9 nucleotides Pf6N2E2_4678 and Pf6N2E2_4679 overlap by 19 nucleotides Pf6N2E2_4679 and Pf6N2E2_4680 are separated by 80 nucleotides
Pf6N2E2_4677 - Choline dehydrogenase (EC 1.1.99.1), at 5,410,297 to 5,412,000
_4677
Pf6N2E2_4678 - hypothetical protein, at 5,411,992 to 5,412,231
_4678
Pf6N2E2_4679 - Betaine aldehyde dehydrogenase (EC 1.2.1.8), at 5,412,213 to 5,413,685
_4679
Pf6N2E2_4680 - HTH-type transcriptional regulator BetI, at 5,413,766 to 5,414,386
_4680
Position (kb)
5412
5413
5414 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5411.384 kb on + strand, within Pf6N2E2_4677 at 5411.385 kb on - strand, within Pf6N2E2_4677 at 5411.497 kb on + strand, within Pf6N2E2_4677 at 5411.498 kb on - strand, within Pf6N2E2_4677 at 5411.625 kb on - strand, within Pf6N2E2_4677 at 5411.625 kb on - strand, within Pf6N2E2_4677 at 5411.642 kb on + strand, within Pf6N2E2_4677 at 5411.642 kb on + strand, within Pf6N2E2_4677 at 5411.643 kb on - strand, within Pf6N2E2_4677 at 5411.643 kb on - strand, within Pf6N2E2_4677 at 5411.643 kb on - strand, within Pf6N2E2_4677 at 5411.684 kb on + strand, within Pf6N2E2_4677 at 5411.685 kb on - strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.708 kb on + strand, within Pf6N2E2_4677 at 5411.709 kb on - strand, within Pf6N2E2_4677 at 5411.709 kb on - strand, within Pf6N2E2_4677 at 5411.709 kb on - strand, within Pf6N2E2_4677 at 5411.709 kb on - strand, within Pf6N2E2_4677 at 5411.709 kb on - strand, within Pf6N2E2_4677 at 5411.805 kb on - strand, within Pf6N2E2_4677 at 5411.981 kb on + strand at 5411.981 kb on + strand at 5411.982 kb on - strand at 5411.989 kb on + strand at 5411.989 kb on + strand at 5411.990 kb on - strand at 5412.099 kb on - strand, within Pf6N2E2_4678 at 5412.249 kb on + strand at 5412.319 kb on + strand at 5412.375 kb on - strand, within Pf6N2E2_4679 at 5412.493 kb on + strand, within Pf6N2E2_4679 at 5412.494 kb on - strand, within Pf6N2E2_4679 at 5412.668 kb on - strand, within Pf6N2E2_4679 at 5412.668 kb on - strand, within Pf6N2E2_4679 at 5412.668 kb on - strand, within Pf6N2E2_4679 at 5412.847 kb on + strand, within Pf6N2E2_4679 at 5412.847 kb on + strand, within Pf6N2E2_4679 at 5412.847 kb on + strand, within Pf6N2E2_4679 at 5412.848 kb on - strand, within Pf6N2E2_4679 at 5412.848 kb on - strand, within Pf6N2E2_4679 at 5413.111 kb on + strand, within Pf6N2E2_4679 at 5413.112 kb on - strand, within Pf6N2E2_4679 at 5413.112 kb on - strand, within Pf6N2E2_4679 at 5413.112 kb on - strand, within Pf6N2E2_4679 at 5413.251 kb on + strand, within Pf6N2E2_4679 at 5413.251 kb on + strand, within Pf6N2E2_4679 at 5413.251 kb on + strand, within Pf6N2E2_4679 at 5413.276 kb on + strand, within Pf6N2E2_4679 at 5413.276 kb on + strand, within Pf6N2E2_4679 at 5413.339 kb on + strand, within Pf6N2E2_4679 at 5413.339 kb on + strand, within Pf6N2E2_4679 at 5413.339 kb on + strand, within Pf6N2E2_4679 at 5413.340 kb on - strand, within Pf6N2E2_4679 at 5413.340 kb on - strand, within Pf6N2E2_4679 at 5413.342 kb on + strand, within Pf6N2E2_4679 at 5413.343 kb on - strand, within Pf6N2E2_4679 at 5413.343 kb on - strand, within Pf6N2E2_4679 at 5413.343 kb on - strand, within Pf6N2E2_4679 at 5413.343 kb on - strand, within Pf6N2E2_4679 at 5413.343 kb on - strand, within Pf6N2E2_4679 at 5413.393 kb on + strand, within Pf6N2E2_4679 at 5413.642 kb on + strand at 5413.642 kb on + strand at 5413.657 kb on + strand at 5413.658 kb on - strand at 5413.658 kb on - strand at 5413.704 kb on + strand at 5413.704 kb on + strand at 5413.704 kb on + strand at 5413.704 kb on + strand at 5413.704 kb on + strand at 5413.704 kb on + strand at 5413.704 kb on + strand at 5413.705 kb on - strand at 5413.705 kb on - strand at 5413.705 kb on - strand at 5413.705 kb on - strand at 5413.705 kb on - strand at 5413.705 kb on - strand at 5413.705 kb on - strand at 5413.737 kb on + strand at 5413.737 kb on + strand at 5413.738 kb on - strand at 5413.739 kb on + strand at 5413.740 kb on - strand at 5413.827 kb on + strand at 5413.827 kb on + strand at 5413.827 kb on + strand at 5413.828 kb on - strand, within Pf6N2E2_4680 at 5413.828 kb on - strand, within Pf6N2E2_4680 at 5413.828 kb on - strand, within Pf6N2E2_4680 at 5413.834 kb on - strand, within Pf6N2E2_4680 at 5413.986 kb on + strand, within Pf6N2E2_4680 at 5413.987 kb on - strand, within Pf6N2E2_4680 at 5414.388 kb on + strand at 5414.388 kb on + strand at 5414.388 kb on + strand at 5414.388 kb on + strand at 5414.388 kb on + strand at 5414.388 kb on + strand at 5414.388 kb on + strand at 5414.389 kb on - strand at 5414.389 kb on - strand at 5414.389 kb on - strand at 5414.389 kb on - strand at 5414.389 kb on - strand at 5414.389 kb on - strand at 5414.389 kb on - strand at 5414.389 kb on - strand at 5414.389 kb on - strand at 5414.389 kb on - strand at 5414.389 kb on - strand at 5414.401 kb on + strand at 5414.402 kb on - strand at 5414.448 kb on + strand at 5414.449 kb on - strand at 5414.491 kb on + strand at 5414.491 kb on + strand at 5414.529 kb on + strand at 5414.529 kb on + strand at 5414.529 kb on + strand at 5414.530 kb on - strand at 5414.530 kb on - strand at 5414.594 kb on + strand at 5414.594 kb on + strand at 5414.594 kb on + strand at 5414.595 kb on - strand at 5414.595 kb on - strand at 5414.595 kb on - strand at 5414.595 kb on - strand at 5414.595 kb on - strand at 5414.596 kb on + strand at 5414.596 kb on + strand at 5414.596 kb on + strand at 5414.596 kb on + strand at 5414.596 kb on + strand at 5414.596 kb on + strand at 5414.596 kb on + strand at 5414.596 kb on + strand at 5414.597 kb on - strand at 5414.597 kb on - strand at 5414.597 kb on - strand at 5414.623 kb on - strand at 5414.636 kb on - strand at 5414.645 kb on - strand at 5414.645 kb on - strand at 5414.645 kb on - strand at 5414.645 kb on - strand at 5414.665 kb on + strand at 5414.665 kb on + strand at 5414.666 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 500 mM remove 5,411,384 + Pf6N2E2_4677 0.64 -1.1 5,411,385 - Pf6N2E2_4677 0.64 -0.3 5,411,497 + Pf6N2E2_4677 0.70 -1.4 5,411,498 - Pf6N2E2_4677 0.70 -0.7 5,411,625 - Pf6N2E2_4677 0.78 -0.4 5,411,625 - Pf6N2E2_4677 0.78 -0.0 5,411,642 + Pf6N2E2_4677 0.79 -0.8 5,411,642 + Pf6N2E2_4677 0.79 +0.7 5,411,643 - Pf6N2E2_4677 0.79 -0.2 5,411,643 - Pf6N2E2_4677 0.79 -2.7 5,411,643 - Pf6N2E2_4677 0.79 -0.8 5,411,684 + Pf6N2E2_4677 0.81 +0.4 5,411,685 - Pf6N2E2_4677 0.81 +0.6 5,411,708 + Pf6N2E2_4677 0.83 -1.1 5,411,708 + Pf6N2E2_4677 0.83 +0.2 5,411,708 + Pf6N2E2_4677 0.83 -0.9 5,411,708 + Pf6N2E2_4677 0.83 -0.3 5,411,708 + Pf6N2E2_4677 0.83 -1.7 5,411,708 + Pf6N2E2_4677 0.83 -0.0 5,411,708 + Pf6N2E2_4677 0.83 -0.0 5,411,708 + Pf6N2E2_4677 0.83 -0.1 5,411,709 - Pf6N2E2_4677 0.83 -0.2 5,411,709 - Pf6N2E2_4677 0.83 +0.1 5,411,709 - Pf6N2E2_4677 0.83 -3.0 5,411,709 - Pf6N2E2_4677 0.83 -0.9 5,411,709 - Pf6N2E2_4677 0.83 -0.4 5,411,805 - Pf6N2E2_4677 0.88 -0.3 5,411,981 + +0.3 5,411,981 + -1.3 5,411,982 - -0.0 5,411,989 + -1.0 5,411,989 + +0.4 5,411,990 - +0.0 5,412,099 - Pf6N2E2_4678 0.45 +0.7 5,412,249 + -1.2 5,412,319 + -2.8 5,412,375 - Pf6N2E2_4679 0.11 +0.2 5,412,493 + Pf6N2E2_4679 0.19 -2.7 5,412,494 - Pf6N2E2_4679 0.19 -0.8 5,412,668 - Pf6N2E2_4679 0.31 -1.3 5,412,668 - Pf6N2E2_4679 0.31 -2.3 5,412,668 - Pf6N2E2_4679 0.31 -0.7 5,412,847 + Pf6N2E2_4679 0.43 -1.3 5,412,847 + Pf6N2E2_4679 0.43 -0.6 5,412,847 + Pf6N2E2_4679 0.43 -0.5 5,412,848 - Pf6N2E2_4679 0.43 -0.7 5,412,848 - Pf6N2E2_4679 0.43 -1.8 5,413,111 + Pf6N2E2_4679 0.61 -0.8 5,413,112 - Pf6N2E2_4679 0.61 -1.3 5,413,112 - Pf6N2E2_4679 0.61 -0.4 5,413,112 - Pf6N2E2_4679 0.61 -1.1 5,413,251 + Pf6N2E2_4679 0.70 -2.8 5,413,251 + Pf6N2E2_4679 0.70 -1.1 5,413,251 + Pf6N2E2_4679 0.70 -1.8 5,413,276 + Pf6N2E2_4679 0.72 -2.1 5,413,276 + Pf6N2E2_4679 0.72 -1.5 5,413,339 + Pf6N2E2_4679 0.76 +0.2 5,413,339 + Pf6N2E2_4679 0.76 -2.2 5,413,339 + Pf6N2E2_4679 0.76 -0.9 5,413,340 - Pf6N2E2_4679 0.77 -0.8 5,413,340 - Pf6N2E2_4679 0.77 +0.2 5,413,342 + Pf6N2E2_4679 0.77 -0.3 5,413,343 - Pf6N2E2_4679 0.77 -0.0 5,413,343 - Pf6N2E2_4679 0.77 -1.6 5,413,343 - Pf6N2E2_4679 0.77 -0.6 5,413,343 - Pf6N2E2_4679 0.77 -1.0 5,413,343 - Pf6N2E2_4679 0.77 +0.2 5,413,393 + Pf6N2E2_4679 0.80 -0.8 5,413,642 + +0.6 5,413,642 + +0.4 5,413,657 + +0.2 5,413,658 - -1.6 5,413,658 - -1.6 5,413,704 + -0.7 5,413,704 + -0.8 5,413,704 + +0.6 5,413,704 + -0.2 5,413,704 + -0.2 5,413,704 + -1.8 5,413,704 + -1.4 5,413,705 - -0.8 5,413,705 - +0.5 5,413,705 - -0.2 5,413,705 - -0.7 5,413,705 - -1.7 5,413,705 - +0.9 5,413,705 - +1.1 5,413,737 + -1.1 5,413,737 + +0.2 5,413,738 - +0.5 5,413,739 + -0.5 5,413,740 - +1.6 5,413,827 + -1.3 5,413,827 + +0.5 5,413,827 + -1.1 5,413,828 - Pf6N2E2_4680 0.10 +0.7 5,413,828 - Pf6N2E2_4680 0.10 +0.9 5,413,828 - Pf6N2E2_4680 0.10 +2.3 5,413,834 - Pf6N2E2_4680 0.11 +0.5 5,413,986 + Pf6N2E2_4680 0.35 -0.5 5,413,987 - Pf6N2E2_4680 0.36 +0.9 5,414,388 + -0.2 5,414,388 + -2.8 5,414,388 + +0.2 5,414,388 + +1.2 5,414,388 + +0.5 5,414,388 + +0.2 5,414,388 + -1.0 5,414,389 - +0.9 5,414,389 - +1.4 5,414,389 - +1.2 5,414,389 - +1.1 5,414,389 - -0.5 5,414,389 - +0.9 5,414,389 - -1.1 5,414,389 - +0.6 5,414,389 - +0.6 5,414,389 - +0.0 5,414,389 - +0.3 5,414,401 + -1.1 5,414,402 - -0.6 5,414,448 + -0.2 5,414,449 - +0.7 5,414,491 + -1.0 5,414,491 + +0.2 5,414,529 + +0.8 5,414,529 + -1.2 5,414,529 + +1.0 5,414,530 - -0.5 5,414,530 - -1.3 5,414,594 + -0.7 5,414,594 + +1.6 5,414,594 + +0.2 5,414,595 - -0.2 5,414,595 - -0.7 5,414,595 - -0.1 5,414,595 - -2.5 5,414,595 - -1.3 5,414,596 + +0.4 5,414,596 + +0.2 5,414,596 + -0.1 5,414,596 + +0.1 5,414,596 + +0.1 5,414,596 + +0.7 5,414,596 + -1.8 5,414,596 + -0.2 5,414,597 - -0.7 5,414,597 - +0.4 5,414,597 - +0.7 5,414,623 - -1.0 5,414,636 - +0.1 5,414,645 - -0.2 5,414,645 - +1.0 5,414,645 - -0.1 5,414,645 - +1.6 5,414,665 + +0.3 5,414,665 + +0.7 5,414,666 - -1.6
Or see this region's nucleotide sequence