Experiment: Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2367 and Pf6N2E2_2368 overlap by 4 nucleotides Pf6N2E2_2368 and Pf6N2E2_2369 overlap by 17 nucleotides Pf6N2E2_2369 and Pf6N2E2_2370 are separated by 59 nucleotides
Pf6N2E2_2367 - hypothetical protein, at 2,800,995 to 2,801,870
_2367
Pf6N2E2_2368 - Surface presentation of antigens protein SpaM, at 2,801,867 to 2,802,322
_2368
Pf6N2E2_2369 - Flagellum-specific ATP synthase FliI, at 2,802,306 to 2,803,556
_2369
Pf6N2E2_2370 - Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV), at 2,803,616 to 2,805,673
_2370
Position (kb)
2802
2803
2804 Strain fitness (log2 ratio)
-1
0
1
2 at 2801.664 kb on - strand, within Pf6N2E2_2367 at 2801.664 kb on - strand, within Pf6N2E2_2367 at 2802.616 kb on + strand, within Pf6N2E2_2369 at 2802.617 kb on - strand, within Pf6N2E2_2369 at 2802.694 kb on + strand, within Pf6N2E2_2369 at 2802.695 kb on - strand, within Pf6N2E2_2369 at 2802.934 kb on - strand, within Pf6N2E2_2369 at 2803.158 kb on + strand, within Pf6N2E2_2369 at 2803.159 kb on - strand, within Pf6N2E2_2369 at 2803.533 kb on + strand at 2803.533 kb on + strand at 2803.534 kb on - strand at 2803.590 kb on - strand at 2803.590 kb on - strand at 2803.944 kb on + strand, within Pf6N2E2_2370 at 2804.112 kb on + strand, within Pf6N2E2_2370 at 2804.260 kb on - strand, within Pf6N2E2_2370 at 2804.505 kb on + strand, within Pf6N2E2_2370
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 500 mM remove 2,801,664 - Pf6N2E2_2367 0.76 -1.0 2,801,664 - Pf6N2E2_2367 0.76 -0.8 2,802,616 + Pf6N2E2_2369 0.25 +1.8 2,802,617 - Pf6N2E2_2369 0.25 +0.6 2,802,694 + Pf6N2E2_2369 0.31 +0.0 2,802,695 - Pf6N2E2_2369 0.31 +1.3 2,802,934 - Pf6N2E2_2369 0.50 -0.5 2,803,158 + Pf6N2E2_2369 0.68 +0.7 2,803,159 - Pf6N2E2_2369 0.68 -0.8 2,803,533 + +0.1 2,803,533 + +1.1 2,803,534 - +0.0 2,803,590 - -0.7 2,803,590 - -0.5 2,803,944 + Pf6N2E2_2370 0.16 -0.4 2,804,112 + Pf6N2E2_2370 0.24 +0.9 2,804,260 - Pf6N2E2_2370 0.31 +1.1 2,804,505 + Pf6N2E2_2370 0.43 -0.7
Or see this region's nucleotide sequence