Experiment: Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2235 and Pf6N2E2_2236 are separated by 124 nucleotides Pf6N2E2_2236 and Pf6N2E2_2237 are separated by 117 nucleotides Pf6N2E2_2237 and Pf6N2E2_2238 are separated by 69 nucleotides
Pf6N2E2_2235 - Xanthine permease, at 2,647,023 to 2,648,543
_2235
Pf6N2E2_2236 - N-hydroxyarylamine O-acetyltransferase (EC 2.3.1.118), at 2,648,668 to 2,649,492
_2236
Pf6N2E2_2237 - Exodeoxyribonuclease III (EC 3.1.11.2), at 2,649,610 to 2,650,404
_2237
Pf6N2E2_2238 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 2,650,474 to 2,653,050
_2238
Position (kb)
2648
2649
2650 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2647.980 kb on + strand, within Pf6N2E2_2235 at 2647.981 kb on - strand, within Pf6N2E2_2235 at 2648.446 kb on + strand at 2648.446 kb on + strand at 2648.446 kb on + strand at 2648.446 kb on + strand at 2648.446 kb on + strand at 2648.447 kb on - strand at 2648.447 kb on - strand at 2648.447 kb on - strand at 2648.474 kb on - strand at 2648.480 kb on + strand at 2648.481 kb on - strand at 2648.610 kb on + strand at 2648.669 kb on + strand at 2648.670 kb on - strand at 2648.816 kb on + strand, within Pf6N2E2_2236 at 2648.817 kb on - strand, within Pf6N2E2_2236 at 2648.910 kb on - strand, within Pf6N2E2_2236 at 2648.910 kb on - strand, within Pf6N2E2_2236 at 2648.927 kb on + strand, within Pf6N2E2_2236 at 2648.928 kb on - strand, within Pf6N2E2_2236 at 2648.963 kb on + strand, within Pf6N2E2_2236 at 2648.963 kb on + strand, within Pf6N2E2_2236 at 2648.964 kb on - strand, within Pf6N2E2_2236 at 2649.032 kb on + strand, within Pf6N2E2_2236 at 2649.122 kb on + strand, within Pf6N2E2_2236 at 2649.122 kb on + strand, within Pf6N2E2_2236 at 2649.122 kb on + strand, within Pf6N2E2_2236 at 2649.122 kb on + strand, within Pf6N2E2_2236 at 2649.123 kb on - strand, within Pf6N2E2_2236 at 2649.136 kb on + strand, within Pf6N2E2_2236 at 2649.137 kb on - strand, within Pf6N2E2_2236 at 2649.272 kb on + strand, within Pf6N2E2_2236 at 2649.272 kb on + strand, within Pf6N2E2_2236 at 2649.272 kb on + strand, within Pf6N2E2_2236 at 2649.272 kb on + strand, within Pf6N2E2_2236 at 2649.273 kb on - strand, within Pf6N2E2_2236 at 2649.273 kb on - strand, within Pf6N2E2_2236 at 2649.384 kb on + strand, within Pf6N2E2_2236 at 2649.458 kb on + strand at 2649.558 kb on + strand at 2649.558 kb on + strand at 2649.558 kb on + strand at 2649.558 kb on + strand at 2649.558 kb on + strand at 2649.559 kb on - strand at 2649.559 kb on - strand at 2649.559 kb on - strand at 2649.560 kb on + strand at 2649.560 kb on + strand at 2649.560 kb on + strand at 2649.560 kb on + strand at 2649.560 kb on + strand at 2649.561 kb on - strand at 2649.561 kb on - strand at 2649.561 kb on - strand at 2649.561 kb on - strand at 2649.711 kb on - strand, within Pf6N2E2_2237 at 2649.734 kb on - strand, within Pf6N2E2_2237 at 2649.801 kb on - strand, within Pf6N2E2_2237 at 2649.819 kb on - strand, within Pf6N2E2_2237 at 2649.923 kb on + strand, within Pf6N2E2_2237 at 2649.923 kb on + strand, within Pf6N2E2_2237 at 2649.923 kb on + strand, within Pf6N2E2_2237 at 2649.923 kb on + strand, within Pf6N2E2_2237 at 2649.924 kb on - strand, within Pf6N2E2_2237 at 2649.924 kb on - strand, within Pf6N2E2_2237 at 2649.924 kb on - strand, within Pf6N2E2_2237 at 2649.924 kb on - strand, within Pf6N2E2_2237 at 2649.924 kb on - strand, within Pf6N2E2_2237 at 2649.924 kb on - strand, within Pf6N2E2_2237 at 2649.953 kb on + strand, within Pf6N2E2_2237 at 2649.953 kb on + strand, within Pf6N2E2_2237 at 2649.953 kb on + strand, within Pf6N2E2_2237 at 2649.954 kb on - strand, within Pf6N2E2_2237 at 2649.954 kb on - strand, within Pf6N2E2_2237 at 2649.954 kb on - strand, within Pf6N2E2_2237 at 2650.037 kb on + strand, within Pf6N2E2_2237 at 2650.037 kb on + strand, within Pf6N2E2_2237 at 2650.038 kb on - strand, within Pf6N2E2_2237 at 2650.038 kb on - strand, within Pf6N2E2_2237 at 2650.038 kb on - strand, within Pf6N2E2_2237 at 2650.079 kb on + strand, within Pf6N2E2_2237 at 2650.079 kb on + strand, within Pf6N2E2_2237 at 2650.079 kb on + strand, within Pf6N2E2_2237 at 2650.079 kb on + strand, within Pf6N2E2_2237 at 2650.080 kb on - strand, within Pf6N2E2_2237 at 2650.374 kb on + strand at 2650.404 kb on + strand at 2650.405 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 500 mM remove 2,647,980 + Pf6N2E2_2235 0.63 -0.3 2,647,981 - Pf6N2E2_2235 0.63 -2.5 2,648,446 + +0.5 2,648,446 + -0.8 2,648,446 + -1.4 2,648,446 + -0.5 2,648,446 + +0.1 2,648,447 - +0.3 2,648,447 - +0.9 2,648,447 - +0.5 2,648,474 - -0.7 2,648,480 + +0.1 2,648,481 - +1.3 2,648,610 + -0.3 2,648,669 + +1.8 2,648,670 - +1.0 2,648,816 + Pf6N2E2_2236 0.18 -1.1 2,648,817 - Pf6N2E2_2236 0.18 -0.6 2,648,910 - Pf6N2E2_2236 0.29 -0.3 2,648,910 - Pf6N2E2_2236 0.29 +0.3 2,648,927 + Pf6N2E2_2236 0.31 -0.3 2,648,928 - Pf6N2E2_2236 0.32 +0.7 2,648,963 + Pf6N2E2_2236 0.36 -1.5 2,648,963 + Pf6N2E2_2236 0.36 +1.6 2,648,964 - Pf6N2E2_2236 0.36 -0.5 2,649,032 + Pf6N2E2_2236 0.44 -1.5 2,649,122 + Pf6N2E2_2236 0.55 -1.0 2,649,122 + Pf6N2E2_2236 0.55 +1.6 2,649,122 + Pf6N2E2_2236 0.55 +0.3 2,649,122 + Pf6N2E2_2236 0.55 +0.3 2,649,123 - Pf6N2E2_2236 0.55 -0.5 2,649,136 + Pf6N2E2_2236 0.57 -1.0 2,649,137 - Pf6N2E2_2236 0.57 -0.5 2,649,272 + Pf6N2E2_2236 0.73 +0.4 2,649,272 + Pf6N2E2_2236 0.73 -0.6 2,649,272 + Pf6N2E2_2236 0.73 -0.1 2,649,272 + Pf6N2E2_2236 0.73 -0.5 2,649,273 - Pf6N2E2_2236 0.73 -0.4 2,649,273 - Pf6N2E2_2236 0.73 -0.4 2,649,384 + Pf6N2E2_2236 0.87 -0.6 2,649,458 + -0.5 2,649,558 + +0.7 2,649,558 + -0.1 2,649,558 + -0.3 2,649,558 + +0.8 2,649,558 + +0.1 2,649,559 - +0.3 2,649,559 - -1.4 2,649,559 - +0.4 2,649,560 + -0.2 2,649,560 + +0.4 2,649,560 + +0.2 2,649,560 + -0.6 2,649,560 + +1.1 2,649,561 - +0.1 2,649,561 - +0.6 2,649,561 - -0.9 2,649,561 - -1.3 2,649,711 - Pf6N2E2_2237 0.13 +0.3 2,649,734 - Pf6N2E2_2237 0.16 +1.8 2,649,801 - Pf6N2E2_2237 0.24 +0.6 2,649,819 - Pf6N2E2_2237 0.26 -0.4 2,649,923 + Pf6N2E2_2237 0.39 -0.7 2,649,923 + Pf6N2E2_2237 0.39 -0.0 2,649,923 + Pf6N2E2_2237 0.39 -0.7 2,649,923 + Pf6N2E2_2237 0.39 +0.9 2,649,924 - Pf6N2E2_2237 0.39 +0.7 2,649,924 - Pf6N2E2_2237 0.39 -0.1 2,649,924 - Pf6N2E2_2237 0.39 -0.4 2,649,924 - Pf6N2E2_2237 0.39 -0.3 2,649,924 - Pf6N2E2_2237 0.39 -0.1 2,649,924 - Pf6N2E2_2237 0.39 -1.6 2,649,953 + Pf6N2E2_2237 0.43 +0.0 2,649,953 + Pf6N2E2_2237 0.43 +0.1 2,649,953 + Pf6N2E2_2237 0.43 -0.9 2,649,954 - Pf6N2E2_2237 0.43 -0.4 2,649,954 - Pf6N2E2_2237 0.43 -0.9 2,649,954 - Pf6N2E2_2237 0.43 +0.8 2,650,037 + Pf6N2E2_2237 0.54 +0.6 2,650,037 + Pf6N2E2_2237 0.54 +0.4 2,650,038 - Pf6N2E2_2237 0.54 +0.1 2,650,038 - Pf6N2E2_2237 0.54 +0.5 2,650,038 - Pf6N2E2_2237 0.54 +0.2 2,650,079 + Pf6N2E2_2237 0.59 -0.8 2,650,079 + Pf6N2E2_2237 0.59 -0.4 2,650,079 + Pf6N2E2_2237 0.59 +0.1 2,650,079 + Pf6N2E2_2237 0.59 -1.1 2,650,080 - Pf6N2E2_2237 0.59 +0.2 2,650,374 + +1.8 2,650,404 + -0.5 2,650,405 - -0.8
Or see this region's nucleotide sequence