Experiment: Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1990 and Pf6N2E2_1991 are separated by 257 nucleotides Pf6N2E2_1991 and Pf6N2E2_1992 are separated by 177 nucleotides Pf6N2E2_1992 and Pf6N2E2_1993 are separated by 160 nucleotides Pf6N2E2_1993 and Pf6N2E2_1994 are separated by 4 nucleotides Pf6N2E2_1994 and Pf6N2E2_1995 overlap by 1 nucleotides
Pf6N2E2_1990 - Possible exported protein, at 2,355,332 to 2,356,015
_1990
Pf6N2E2_1991 - hypothetical protein, at 2,356,273 to 2,356,407
_1991
Pf6N2E2_1992 - hypothetical protein, at 2,356,585 to 2,356,728
_1992
Pf6N2E2_1993 - putative exported protein, at 2,356,889 to 2,358,316
_1993
Pf6N2E2_1994 - putative lipoprotein, at 2,358,321 to 2,358,719
_1994
Pf6N2E2_1995 - Type IV secretory pathway, VirB4 components, at 2,358,719 to 2,361,568
_1995
Position (kb)
2356
2357
2358
2359 Strain fitness (log2 ratio)
-2
-1
0
1 at 2355.977 kb on - strand at 2356.088 kb on + strand at 2356.089 kb on - strand at 2356.162 kb on - strand at 2356.167 kb on - strand at 2356.167 kb on - strand at 2356.167 kb on - strand at 2356.167 kb on - strand at 2356.202 kb on + strand at 2356.232 kb on - strand at 2356.232 kb on - strand at 2356.326 kb on - strand, within Pf6N2E2_1991 at 2356.438 kb on - strand at 2356.439 kb on + strand at 2356.440 kb on - strand at 2356.440 kb on - strand at 2356.440 kb on - strand at 2356.464 kb on - strand at 2356.504 kb on - strand at 2356.511 kb on + strand at 2356.511 kb on + strand at 2356.512 kb on - strand at 2356.512 kb on - strand at 2356.512 kb on - strand at 2356.512 kb on - strand at 2356.568 kb on + strand at 2356.569 kb on - strand at 2356.579 kb on - strand at 2356.627 kb on + strand, within Pf6N2E2_1992 at 2357.283 kb on + strand, within Pf6N2E2_1993 at 2357.308 kb on + strand, within Pf6N2E2_1993 at 2357.309 kb on - strand, within Pf6N2E2_1993 at 2357.421 kb on + strand, within Pf6N2E2_1993 at 2357.508 kb on + strand, within Pf6N2E2_1993 at 2357.570 kb on + strand, within Pf6N2E2_1993 at 2357.570 kb on + strand, within Pf6N2E2_1993 at 2357.570 kb on + strand, within Pf6N2E2_1993 at 2357.573 kb on - strand, within Pf6N2E2_1993 at 2357.677 kb on - strand, within Pf6N2E2_1993 at 2357.749 kb on + strand, within Pf6N2E2_1993 at 2357.812 kb on - strand, within Pf6N2E2_1993 at 2357.975 kb on + strand, within Pf6N2E2_1993 at 2358.054 kb on - strand, within Pf6N2E2_1993 at 2358.167 kb on + strand, within Pf6N2E2_1993 at 2358.192 kb on - strand at 2358.239 kb on - strand at 2358.534 kb on - strand, within Pf6N2E2_1994 at 2358.600 kb on + strand, within Pf6N2E2_1994 at 2358.600 kb on + strand, within Pf6N2E2_1994 at 2358.601 kb on - strand, within Pf6N2E2_1994 at 2358.648 kb on + strand, within Pf6N2E2_1994 at 2358.684 kb on + strand at 2358.714 kb on + strand at 2358.746 kb on + strand at 2358.746 kb on + strand at 2358.746 kb on + strand at 2358.746 kb on + strand at 2358.747 kb on - strand at 2358.747 kb on - strand at 2358.931 kb on - strand at 2359.111 kb on - strand, within Pf6N2E2_1995 at 2359.111 kb on - strand, within Pf6N2E2_1995 at 2359.111 kb on - strand, within Pf6N2E2_1995 at 2359.111 kb on - strand, within Pf6N2E2_1995 at 2359.113 kb on - strand, within Pf6N2E2_1995 at 2359.113 kb on - strand, within Pf6N2E2_1995 at 2359.113 kb on - strand, within Pf6N2E2_1995 at 2359.113 kb on - strand, within Pf6N2E2_1995 at 2359.151 kb on - strand, within Pf6N2E2_1995 at 2359.164 kb on - strand, within Pf6N2E2_1995 at 2359.206 kb on - strand, within Pf6N2E2_1995 at 2359.210 kb on - strand, within Pf6N2E2_1995
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 500 mM remove 2,355,977 - -0.8 2,356,088 + -0.1 2,356,089 - +0.1 2,356,162 - +0.1 2,356,167 - -0.1 2,356,167 - -0.4 2,356,167 - -0.1 2,356,167 - -0.2 2,356,202 + +1.6 2,356,232 - -0.0 2,356,232 - -0.6 2,356,326 - Pf6N2E2_1991 0.39 +0.0 2,356,438 - +0.8 2,356,439 + -1.9 2,356,440 - -0.1 2,356,440 - -2.0 2,356,440 - +0.0 2,356,464 - -0.3 2,356,504 - +0.3 2,356,511 + -1.3 2,356,511 + +0.3 2,356,512 - -0.1 2,356,512 - +1.3 2,356,512 - +1.3 2,356,512 - +0.0 2,356,568 + -0.5 2,356,569 - +0.9 2,356,579 - -0.4 2,356,627 + Pf6N2E2_1992 0.29 -1.3 2,357,283 + Pf6N2E2_1993 0.28 +1.3 2,357,308 + Pf6N2E2_1993 0.29 +0.4 2,357,309 - Pf6N2E2_1993 0.29 +0.3 2,357,421 + Pf6N2E2_1993 0.37 -0.3 2,357,508 + Pf6N2E2_1993 0.43 +0.9 2,357,570 + Pf6N2E2_1993 0.48 +1.3 2,357,570 + Pf6N2E2_1993 0.48 +0.9 2,357,570 + Pf6N2E2_1993 0.48 +1.3 2,357,573 - Pf6N2E2_1993 0.48 +0.7 2,357,677 - Pf6N2E2_1993 0.55 +0.7 2,357,749 + Pf6N2E2_1993 0.60 +0.8 2,357,812 - Pf6N2E2_1993 0.65 +1.0 2,357,975 + Pf6N2E2_1993 0.76 -0.2 2,358,054 - Pf6N2E2_1993 0.82 -0.8 2,358,167 + Pf6N2E2_1993 0.89 +0.5 2,358,192 - +1.1 2,358,239 - +0.6 2,358,534 - Pf6N2E2_1994 0.53 -0.2 2,358,600 + Pf6N2E2_1994 0.70 -0.3 2,358,600 + Pf6N2E2_1994 0.70 +1.1 2,358,601 - Pf6N2E2_1994 0.70 -2.6 2,358,648 + Pf6N2E2_1994 0.82 -2.3 2,358,684 + +0.1 2,358,714 + -0.6 2,358,746 + -0.9 2,358,746 + -0.1 2,358,746 + -0.2 2,358,746 + +0.2 2,358,747 - -1.2 2,358,747 - +0.8 2,358,931 - +0.3 2,359,111 - Pf6N2E2_1995 0.14 +0.5 2,359,111 - Pf6N2E2_1995 0.14 -0.2 2,359,111 - Pf6N2E2_1995 0.14 -0.7 2,359,111 - Pf6N2E2_1995 0.14 +0.8 2,359,113 - Pf6N2E2_1995 0.14 +0.9 2,359,113 - Pf6N2E2_1995 0.14 -0.1 2,359,113 - Pf6N2E2_1995 0.14 -0.2 2,359,113 - Pf6N2E2_1995 0.14 -0.6 2,359,151 - Pf6N2E2_1995 0.15 -0.2 2,359,164 - Pf6N2E2_1995 0.16 +0.5 2,359,206 - Pf6N2E2_1995 0.17 -0.4 2,359,210 - Pf6N2E2_1995 0.17 -1.1
Or see this region's nucleotide sequence