Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1921

Experiment: Chloride 500 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1919 and Pf6N2E2_1920 are separated by 87 nucleotidesPf6N2E2_1920 and Pf6N2E2_1921 are separated by 22 nucleotidesPf6N2E2_1921 and Pf6N2E2_1922 overlap by 4 nucleotides Pf6N2E2_1919 - Aldehyde dehydrogenase (EC 1.2.1.3), at 2,273,106 to 2,274,578 _1919 Pf6N2E2_1920 - Rubredoxin, at 2,274,666 to 2,274,872 _1920 Pf6N2E2_1921 - hypothetical protein, at 2,274,895 to 2,275,032 _1921 Pf6N2E2_1922 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 2,275,029 to 2,277,140 _1922 Position (kb) 2274 2275 2276Strain fitness (log2 ratio) -2 -1 0 1 2at 2273.995 kb on - strand, within Pf6N2E2_1919at 2274.259 kb on - strand, within Pf6N2E2_1919at 2274.259 kb on - strand, within Pf6N2E2_1919at 2274.341 kb on + strand, within Pf6N2E2_1919at 2274.341 kb on + strand, within Pf6N2E2_1919at 2274.341 kb on + strand, within Pf6N2E2_1919at 2274.342 kb on - strand, within Pf6N2E2_1919at 2274.344 kb on + strand, within Pf6N2E2_1919at 2274.345 kb on - strand, within Pf6N2E2_1919at 2274.345 kb on - strand, within Pf6N2E2_1919at 2274.345 kb on - strand, within Pf6N2E2_1919at 2274.557 kb on + strandat 2274.558 kb on - strandat 2274.558 kb on - strandat 2274.558 kb on - strandat 2274.664 kb on - strandat 2274.664 kb on - strandat 2274.760 kb on - strand, within Pf6N2E2_1920at 2274.760 kb on - strand, within Pf6N2E2_1920at 2274.777 kb on - strand, within Pf6N2E2_1920at 2274.777 kb on - strand, within Pf6N2E2_1920at 2274.896 kb on + strandat 2274.935 kb on + strand, within Pf6N2E2_1921at 2275.033 kb on - strandat 2275.033 kb on - strandat 2275.033 kb on - strandat 2275.094 kb on + strandat 2275.115 kb on - strandat 2275.178 kb on - strandat 2275.216 kb on + strandat 2275.257 kb on - strand, within Pf6N2E2_1922at 2275.257 kb on - strand, within Pf6N2E2_1922at 2275.257 kb on - strand, within Pf6N2E2_1922at 2275.299 kb on + strand, within Pf6N2E2_1922at 2275.299 kb on + strand, within Pf6N2E2_1922at 2275.335 kb on - strand, within Pf6N2E2_1922at 2275.430 kb on - strand, within Pf6N2E2_1922at 2275.501 kb on + strand, within Pf6N2E2_1922at 2275.502 kb on - strand, within Pf6N2E2_1922at 2275.541 kb on - strand, within Pf6N2E2_1922at 2275.650 kb on + strand, within Pf6N2E2_1922at 2275.651 kb on - strand, within Pf6N2E2_1922at 2275.975 kb on - strand, within Pf6N2E2_1922at 2276.026 kb on + strand, within Pf6N2E2_1922

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Per-strain Table

Position Strand Gene LocusTag Fraction Chloride 500 mM
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2,273,995 - Pf6N2E2_1919 0.60 +1.5
2,274,259 - Pf6N2E2_1919 0.78 -2.4
2,274,259 - Pf6N2E2_1919 0.78 +0.1
2,274,341 + Pf6N2E2_1919 0.84 -1.2
2,274,341 + Pf6N2E2_1919 0.84 +0.4
2,274,341 + Pf6N2E2_1919 0.84 +0.8
2,274,342 - Pf6N2E2_1919 0.84 +1.4
2,274,344 + Pf6N2E2_1919 0.84 -0.2
2,274,345 - Pf6N2E2_1919 0.84 -1.6
2,274,345 - Pf6N2E2_1919 0.84 -0.2
2,274,345 - Pf6N2E2_1919 0.84 -0.8
2,274,557 + +0.2
2,274,558 - +0.3
2,274,558 - -0.8
2,274,558 - -0.8
2,274,664 - -0.0
2,274,664 - +2.1
2,274,760 - Pf6N2E2_1920 0.45 +0.6
2,274,760 - Pf6N2E2_1920 0.45 +0.1
2,274,777 - Pf6N2E2_1920 0.54 -1.1
2,274,777 - Pf6N2E2_1920 0.54 -0.3
2,274,896 + +0.0
2,274,935 + Pf6N2E2_1921 0.29 +0.4
2,275,033 - +0.8
2,275,033 - +0.2
2,275,033 - +1.6
2,275,094 + +0.2
2,275,115 - +0.5
2,275,178 - -0.3
2,275,216 + +0.5
2,275,257 - Pf6N2E2_1922 0.11 -0.6
2,275,257 - Pf6N2E2_1922 0.11 +0.2
2,275,257 - Pf6N2E2_1922 0.11 -0.9
2,275,299 + Pf6N2E2_1922 0.13 +2.0
2,275,299 + Pf6N2E2_1922 0.13 +0.6
2,275,335 - Pf6N2E2_1922 0.14 -0.8
2,275,430 - Pf6N2E2_1922 0.19 -1.6
2,275,501 + Pf6N2E2_1922 0.22 +0.4
2,275,502 - Pf6N2E2_1922 0.22 -1.0
2,275,541 - Pf6N2E2_1922 0.24 -1.3
2,275,650 + Pf6N2E2_1922 0.29 -0.6
2,275,651 - Pf6N2E2_1922 0.29 -2.0
2,275,975 - Pf6N2E2_1922 0.45 -0.0
2,276,026 + Pf6N2E2_1922 0.47 -0.8

Or see this region's nucleotide sequence