Experiment: Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1611 and Pf6N2E2_1612 overlap by 4 nucleotides Pf6N2E2_1612 and Pf6N2E2_1613 are separated by 186 nucleotides Pf6N2E2_1613 and Pf6N2E2_1614 are separated by 221 nucleotides
Pf6N2E2_1611 - Phosphonate ABC transporter permease protein phnE2 (TC 3.A.1.9.1), at 1,884,982 to 1,885,809
_1611
Pf6N2E2_1612 - Phosphonate ABC transporter permease protein phnE1 (TC 3.A.1.9.1), at 1,885,806 to 1,886,573
_1612
Pf6N2E2_1613 - short-chain dehydrogenase/reductase, at 1,886,760 to 1,887,503
_1613
Pf6N2E2_1614 - Transcriptional regulator, LysR family, at 1,887,725 to 1,888,630
_1614
Position (kb)
1886
1887
1888 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1885.932 kb on + strand, within Pf6N2E2_1612 at 1885.933 kb on - strand, within Pf6N2E2_1612 at 1886.057 kb on - strand, within Pf6N2E2_1612 at 1886.367 kb on + strand, within Pf6N2E2_1612 at 1886.367 kb on + strand, within Pf6N2E2_1612 at 1886.367 kb on + strand, within Pf6N2E2_1612 at 1886.367 kb on + strand, within Pf6N2E2_1612 at 1886.368 kb on - strand, within Pf6N2E2_1612 at 1886.368 kb on - strand, within Pf6N2E2_1612 at 1886.451 kb on + strand, within Pf6N2E2_1612 at 1886.451 kb on + strand, within Pf6N2E2_1612 at 1886.451 kb on + strand, within Pf6N2E2_1612 at 1886.451 kb on + strand, within Pf6N2E2_1612 at 1886.609 kb on - strand at 1886.698 kb on + strand at 1886.745 kb on + strand at 1886.766 kb on - strand at 1886.787 kb on - strand at 1886.791 kb on + strand at 1887.201 kb on + strand, within Pf6N2E2_1613 at 1887.201 kb on + strand, within Pf6N2E2_1613 at 1887.202 kb on - strand, within Pf6N2E2_1613 at 1887.202 kb on - strand, within Pf6N2E2_1613 at 1887.202 kb on - strand, within Pf6N2E2_1613 at 1887.460 kb on - strand at 1887.460 kb on - strand at 1887.489 kb on + strand at 1887.489 kb on + strand at 1887.489 kb on + strand at 1887.491 kb on + strand at 1887.492 kb on - strand at 1887.492 kb on - strand at 1887.492 kb on - strand at 1887.521 kb on + strand at 1887.521 kb on + strand at 1887.522 kb on - strand at 1887.533 kb on + strand at 1887.533 kb on + strand at 1887.533 kb on + strand at 1887.534 kb on - strand at 1887.551 kb on + strand at 1887.551 kb on + strand at 1887.551 kb on + strand at 1887.591 kb on + strand at 1887.879 kb on - strand, within Pf6N2E2_1614 at 1887.879 kb on - strand, within Pf6N2E2_1614 at 1887.965 kb on + strand, within Pf6N2E2_1614 at 1887.965 kb on + strand, within Pf6N2E2_1614 at 1887.966 kb on - strand, within Pf6N2E2_1614 at 1887.969 kb on + strand, within Pf6N2E2_1614 at 1887.970 kb on - strand, within Pf6N2E2_1614 at 1887.970 kb on - strand, within Pf6N2E2_1614 at 1887.970 kb on - strand, within Pf6N2E2_1614 at 1888.208 kb on - strand, within Pf6N2E2_1614 at 1888.336 kb on + strand, within Pf6N2E2_1614 at 1888.452 kb on + strand, within Pf6N2E2_1614 at 1888.453 kb on - strand, within Pf6N2E2_1614 at 1888.456 kb on - strand, within Pf6N2E2_1614
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 500 mM remove 1,885,932 + Pf6N2E2_1612 0.16 +1.1 1,885,933 - Pf6N2E2_1612 0.17 -0.9 1,886,057 - Pf6N2E2_1612 0.33 -0.6 1,886,367 + Pf6N2E2_1612 0.73 +0.3 1,886,367 + Pf6N2E2_1612 0.73 +1.2 1,886,367 + Pf6N2E2_1612 0.73 -0.3 1,886,367 + Pf6N2E2_1612 0.73 +1.0 1,886,368 - Pf6N2E2_1612 0.73 +0.6 1,886,368 - Pf6N2E2_1612 0.73 +0.4 1,886,451 + Pf6N2E2_1612 0.84 -0.2 1,886,451 + Pf6N2E2_1612 0.84 -1.5 1,886,451 + Pf6N2E2_1612 0.84 -0.0 1,886,451 + Pf6N2E2_1612 0.84 +0.3 1,886,609 - +1.2 1,886,698 + +0.9 1,886,745 + -0.2 1,886,766 - +0.4 1,886,787 - -2.9 1,886,791 + -0.7 1,887,201 + Pf6N2E2_1613 0.59 +1.0 1,887,201 + Pf6N2E2_1613 0.59 -0.1 1,887,202 - Pf6N2E2_1613 0.59 +0.4 1,887,202 - Pf6N2E2_1613 0.59 +0.8 1,887,202 - Pf6N2E2_1613 0.59 +2.1 1,887,460 - -0.9 1,887,460 - +0.6 1,887,489 + -0.1 1,887,489 + +1.2 1,887,489 + -0.1 1,887,491 + -1.3 1,887,492 - -0.4 1,887,492 - -0.9 1,887,492 - -0.5 1,887,521 + -0.8 1,887,521 + +1.5 1,887,522 - -0.5 1,887,533 + -1.3 1,887,533 + -0.2 1,887,533 + -0.2 1,887,534 - -0.5 1,887,551 + +1.0 1,887,551 + -0.3 1,887,551 + -0.3 1,887,591 + +0.7 1,887,879 - Pf6N2E2_1614 0.17 -2.1 1,887,879 - Pf6N2E2_1614 0.17 +0.6 1,887,965 + Pf6N2E2_1614 0.26 -0.1 1,887,965 + Pf6N2E2_1614 0.26 -1.4 1,887,966 - Pf6N2E2_1614 0.27 -0.3 1,887,969 + Pf6N2E2_1614 0.27 -0.6 1,887,970 - Pf6N2E2_1614 0.27 +1.0 1,887,970 - Pf6N2E2_1614 0.27 +0.6 1,887,970 - Pf6N2E2_1614 0.27 +0.4 1,888,208 - Pf6N2E2_1614 0.53 -0.1 1,888,336 + Pf6N2E2_1614 0.67 -0.1 1,888,452 + Pf6N2E2_1614 0.80 -0.1 1,888,453 - Pf6N2E2_1614 0.80 +0.4 1,888,456 - Pf6N2E2_1614 0.81 +0.1
Or see this region's nucleotide sequence