Experiment: Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1368 and Pf6N2E2_1369 are separated by 87 nucleotides Pf6N2E2_1369 and Pf6N2E2_1370 are separated by 69 nucleotides Pf6N2E2_1370 and Pf6N2E2_1371 overlap by 21 nucleotides Pf6N2E2_1371 and Pf6N2E2_1372 are separated by 2 nucleotides
Pf6N2E2_1368 - FIG067310: hypothetical protein, at 1,604,533 to 1,606,182
_1368
Pf6N2E2_1369 - 2,3-dihydroxy-2,3-dihydro-phenylpropionate dehydrogenase (EC 1.3.1.-), at 1,606,270 to 1,607,037
_1369
Pf6N2E2_1370 - Putative benzaldehyde dehydrogenase oxidoreductase protein (EC 1.2.1.28), at 1,607,107 to 1,608,579
_1370
Pf6N2E2_1371 - hypothetical protein, at 1,608,559 to 1,608,861
_1371
Pf6N2E2_1372 - sigma-54 dependent transcriptional regulator, at 1,608,864 to 1,610,639
_1372
Position (kb)
1607
1608
1609 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1606.126 kb on + strand at 1606.196 kb on + strand at 1606.202 kb on + strand at 1606.258 kb on - strand at 1606.277 kb on + strand at 1606.277 kb on + strand at 1606.278 kb on - strand at 1606.379 kb on + strand, within Pf6N2E2_1369 at 1606.380 kb on - strand, within Pf6N2E2_1369 at 1606.532 kb on + strand, within Pf6N2E2_1369 at 1606.533 kb on - strand, within Pf6N2E2_1369 at 1606.562 kb on + strand, within Pf6N2E2_1369 at 1606.763 kb on + strand, within Pf6N2E2_1369 at 1606.763 kb on + strand, within Pf6N2E2_1369 at 1606.763 kb on + strand, within Pf6N2E2_1369 at 1606.763 kb on + strand, within Pf6N2E2_1369 at 1606.763 kb on + strand, within Pf6N2E2_1369 at 1606.763 kb on + strand, within Pf6N2E2_1369 at 1606.763 kb on + strand, within Pf6N2E2_1369 at 1606.764 kb on - strand, within Pf6N2E2_1369 at 1606.764 kb on - strand, within Pf6N2E2_1369 at 1606.764 kb on - strand, within Pf6N2E2_1369 at 1606.764 kb on - strand, within Pf6N2E2_1369 at 1606.764 kb on - strand, within Pf6N2E2_1369 at 1607.050 kb on + strand at 1607.051 kb on - strand at 1607.053 kb on + strand at 1607.054 kb on - strand at 1607.063 kb on + strand at 1607.096 kb on - strand at 1607.162 kb on + strand at 1607.162 kb on + strand at 1607.163 kb on - strand at 1607.163 kb on - strand at 1607.349 kb on - strand, within Pf6N2E2_1370 at 1607.480 kb on + strand, within Pf6N2E2_1370 at 1607.880 kb on + strand, within Pf6N2E2_1370 at 1607.901 kb on + strand, within Pf6N2E2_1370 at 1608.152 kb on + strand, within Pf6N2E2_1370 at 1608.152 kb on + strand, within Pf6N2E2_1370 at 1608.152 kb on + strand, within Pf6N2E2_1370 at 1608.293 kb on + strand, within Pf6N2E2_1370 at 1608.294 kb on - strand, within Pf6N2E2_1370 at 1608.294 kb on - strand, within Pf6N2E2_1370 at 1608.294 kb on - strand, within Pf6N2E2_1370 at 1608.582 kb on - strand at 1608.631 kb on - strand, within Pf6N2E2_1371 at 1608.696 kb on - strand, within Pf6N2E2_1371 at 1608.810 kb on - strand, within Pf6N2E2_1371 at 1608.816 kb on + strand, within Pf6N2E2_1371 at 1608.817 kb on - strand, within Pf6N2E2_1371 at 1608.859 kb on + strand at 1608.860 kb on - strand at 1608.860 kb on - strand at 1609.013 kb on + strand at 1609.014 kb on - strand at 1609.201 kb on + strand, within Pf6N2E2_1372 at 1609.290 kb on + strand, within Pf6N2E2_1372 at 1609.290 kb on + strand, within Pf6N2E2_1372 at 1609.290 kb on + strand, within Pf6N2E2_1372 at 1609.291 kb on - strand, within Pf6N2E2_1372 at 1609.291 kb on - strand, within Pf6N2E2_1372 at 1609.291 kb on - strand, within Pf6N2E2_1372 at 1609.291 kb on - strand, within Pf6N2E2_1372 at 1609.291 kb on - strand, within Pf6N2E2_1372 at 1609.291 kb on - strand, within Pf6N2E2_1372 at 1609.347 kb on + strand, within Pf6N2E2_1372 at 1609.348 kb on - strand, within Pf6N2E2_1372 at 1609.389 kb on + strand, within Pf6N2E2_1372 at 1609.531 kb on - strand, within Pf6N2E2_1372
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 500 mM remove 1,606,126 + -0.9 1,606,196 + -0.0 1,606,202 + -0.1 1,606,258 - -0.6 1,606,277 + +1.1 1,606,277 + +1.3 1,606,278 - +0.2 1,606,379 + Pf6N2E2_1369 0.14 -1.1 1,606,380 - Pf6N2E2_1369 0.14 -0.2 1,606,532 + Pf6N2E2_1369 0.34 -0.1 1,606,533 - Pf6N2E2_1369 0.34 -0.8 1,606,562 + Pf6N2E2_1369 0.38 -0.5 1,606,763 + Pf6N2E2_1369 0.64 +0.5 1,606,763 + Pf6N2E2_1369 0.64 +2.1 1,606,763 + Pf6N2E2_1369 0.64 -0.5 1,606,763 + Pf6N2E2_1369 0.64 +0.6 1,606,763 + Pf6N2E2_1369 0.64 -0.6 1,606,763 + Pf6N2E2_1369 0.64 -1.1 1,606,763 + Pf6N2E2_1369 0.64 -2.9 1,606,764 - Pf6N2E2_1369 0.64 +0.2 1,606,764 - Pf6N2E2_1369 0.64 -1.6 1,606,764 - Pf6N2E2_1369 0.64 +0.1 1,606,764 - Pf6N2E2_1369 0.64 +0.1 1,606,764 - Pf6N2E2_1369 0.64 +0.4 1,607,050 + -1.5 1,607,051 - +0.8 1,607,053 + -0.6 1,607,054 - +0.6 1,607,063 + -0.2 1,607,096 - -0.4 1,607,162 + -0.4 1,607,162 + +0.6 1,607,163 - +0.1 1,607,163 - -0.2 1,607,349 - Pf6N2E2_1370 0.16 -1.6 1,607,480 + Pf6N2E2_1370 0.25 -0.4 1,607,880 + Pf6N2E2_1370 0.52 -0.7 1,607,901 + Pf6N2E2_1370 0.54 -0.9 1,608,152 + Pf6N2E2_1370 0.71 -0.8 1,608,152 + Pf6N2E2_1370 0.71 -2.0 1,608,152 + Pf6N2E2_1370 0.71 -0.1 1,608,293 + Pf6N2E2_1370 0.81 -0.6 1,608,294 - Pf6N2E2_1370 0.81 -1.7 1,608,294 - Pf6N2E2_1370 0.81 -0.6 1,608,294 - Pf6N2E2_1370 0.81 -1.3 1,608,582 - -1.0 1,608,631 - Pf6N2E2_1371 0.24 +1.0 1,608,696 - Pf6N2E2_1371 0.45 +0.1 1,608,810 - Pf6N2E2_1371 0.83 +2.0 1,608,816 + Pf6N2E2_1371 0.85 -0.2 1,608,817 - Pf6N2E2_1371 0.85 +1.4 1,608,859 + -0.8 1,608,860 - +0.6 1,608,860 - +0.2 1,609,013 + -0.9 1,609,014 - -0.0 1,609,201 + Pf6N2E2_1372 0.19 +2.1 1,609,290 + Pf6N2E2_1372 0.24 +0.4 1,609,290 + Pf6N2E2_1372 0.24 -0.9 1,609,290 + Pf6N2E2_1372 0.24 -0.0 1,609,291 - Pf6N2E2_1372 0.24 +0.6 1,609,291 - Pf6N2E2_1372 0.24 +0.7 1,609,291 - Pf6N2E2_1372 0.24 +0.3 1,609,291 - Pf6N2E2_1372 0.24 -0.4 1,609,291 - Pf6N2E2_1372 0.24 -0.0 1,609,291 - Pf6N2E2_1372 0.24 -0.0 1,609,347 + Pf6N2E2_1372 0.27 +0.5 1,609,348 - Pf6N2E2_1372 0.27 +0.6 1,609,389 + Pf6N2E2_1372 0.30 -0.0 1,609,531 - Pf6N2E2_1372 0.38 -1.6
Or see this region's nucleotide sequence