Experiment: Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1300 and Pf6N2E2_1301 are separated by 157 nucleotides Pf6N2E2_1301 and Pf6N2E2_1302 are separated by 50 nucleotides
Pf6N2E2_1300 - Cyclohexadienyl dehydratase (EC 4.2.1.51)(EC 4.2.1.91), at 1,535,128 to 1,535,919
_1300
Pf6N2E2_1301 - TRAP-type C4-dicarboxylate transport system, periplasmic component, at 1,536,077 to 1,537,096
_1301
Pf6N2E2_1302 - TRAP-type C4-dicarboxylate transport system, large permease component, at 1,537,147 to 1,538,427
_1302
Position (kb)
1536
1537
1538 Strain fitness (log2 ratio)
-1
0
1
2 at 1535.078 kb on + strand at 1535.081 kb on + strand at 1535.081 kb on + strand at 1535.082 kb on - strand at 1535.082 kb on - strand at 1535.082 kb on - strand at 1535.139 kb on - strand at 1535.273 kb on - strand, within Pf6N2E2_1300 at 1535.273 kb on - strand, within Pf6N2E2_1300 at 1535.273 kb on - strand, within Pf6N2E2_1300 at 1535.274 kb on + strand, within Pf6N2E2_1300 at 1535.274 kb on + strand, within Pf6N2E2_1300 at 1535.274 kb on + strand, within Pf6N2E2_1300 at 1535.275 kb on - strand, within Pf6N2E2_1300 at 1535.467 kb on + strand, within Pf6N2E2_1300 at 1535.779 kb on + strand, within Pf6N2E2_1300 at 1535.830 kb on + strand, within Pf6N2E2_1300 at 1535.830 kb on + strand, within Pf6N2E2_1300 at 1535.830 kb on + strand, within Pf6N2E2_1300 at 1535.830 kb on + strand, within Pf6N2E2_1300 at 1535.830 kb on + strand, within Pf6N2E2_1300 at 1535.830 kb on + strand, within Pf6N2E2_1300 at 1535.830 kb on + strand, within Pf6N2E2_1300 at 1535.831 kb on - strand, within Pf6N2E2_1300 at 1536.111 kb on + strand at 1536.112 kb on - strand at 1536.112 kb on - strand at 1536.405 kb on + strand, within Pf6N2E2_1301 at 1536.405 kb on + strand, within Pf6N2E2_1301 at 1536.406 kb on - strand, within Pf6N2E2_1301 at 1536.421 kb on - strand, within Pf6N2E2_1301 at 1536.492 kb on + strand, within Pf6N2E2_1301 at 1536.492 kb on + strand, within Pf6N2E2_1301 at 1536.619 kb on + strand, within Pf6N2E2_1301 at 1536.619 kb on + strand, within Pf6N2E2_1301 at 1536.619 kb on + strand, within Pf6N2E2_1301 at 1536.619 kb on + strand, within Pf6N2E2_1301 at 1536.620 kb on - strand, within Pf6N2E2_1301 at 1536.620 kb on - strand, within Pf6N2E2_1301 at 1536.778 kb on - strand at 1536.778 kb on - strand, within Pf6N2E2_1301 at 1537.113 kb on + strand at 1537.113 kb on + strand at 1537.122 kb on - strand at 1537.634 kb on + strand, within Pf6N2E2_1302 at 1537.634 kb on + strand, within Pf6N2E2_1302 at 1537.635 kb on - strand, within Pf6N2E2_1302 at 1537.635 kb on - strand, within Pf6N2E2_1302 at 1537.856 kb on + strand, within Pf6N2E2_1302 at 1537.856 kb on + strand, within Pf6N2E2_1302 at 1537.856 kb on + strand, within Pf6N2E2_1302 at 1537.856 kb on + strand, within Pf6N2E2_1302 at 1537.856 kb on + strand, within Pf6N2E2_1302 at 1537.857 kb on - strand, within Pf6N2E2_1302
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 500 mM remove 1,535,078 + +0.7 1,535,081 + -1.3 1,535,081 + -0.9 1,535,082 - +1.4 1,535,082 - -0.3 1,535,082 - -1.3 1,535,139 - +1.1 1,535,273 - Pf6N2E2_1300 0.18 +0.2 1,535,273 - Pf6N2E2_1300 0.18 +0.8 1,535,273 - Pf6N2E2_1300 0.18 +1.8 1,535,274 + Pf6N2E2_1300 0.18 +0.4 1,535,274 + Pf6N2E2_1300 0.18 +0.5 1,535,274 + Pf6N2E2_1300 0.18 -1.1 1,535,275 - Pf6N2E2_1300 0.19 +1.6 1,535,467 + Pf6N2E2_1300 0.43 +1.3 1,535,779 + Pf6N2E2_1300 0.82 -0.4 1,535,830 + Pf6N2E2_1300 0.89 -0.0 1,535,830 + Pf6N2E2_1300 0.89 -0.0 1,535,830 + Pf6N2E2_1300 0.89 -0.1 1,535,830 + Pf6N2E2_1300 0.89 +0.4 1,535,830 + Pf6N2E2_1300 0.89 -0.5 1,535,830 + Pf6N2E2_1300 0.89 -0.2 1,535,830 + Pf6N2E2_1300 0.89 +0.7 1,535,831 - Pf6N2E2_1300 0.89 -0.6 1,536,111 + +1.5 1,536,112 - -1.6 1,536,112 - +0.7 1,536,405 + Pf6N2E2_1301 0.32 +1.0 1,536,405 + Pf6N2E2_1301 0.32 -0.5 1,536,406 - Pf6N2E2_1301 0.32 +0.8 1,536,421 - Pf6N2E2_1301 0.34 +0.7 1,536,492 + Pf6N2E2_1301 0.41 -0.4 1,536,492 + Pf6N2E2_1301 0.41 +0.7 1,536,619 + Pf6N2E2_1301 0.53 -0.0 1,536,619 + Pf6N2E2_1301 0.53 +0.7 1,536,619 + Pf6N2E2_1301 0.53 +0.8 1,536,619 + Pf6N2E2_1301 0.53 -0.0 1,536,620 - Pf6N2E2_1301 0.53 +0.8 1,536,620 - Pf6N2E2_1301 0.53 +1.2 1,536,778 - +1.6 1,536,778 - Pf6N2E2_1301 0.69 +0.4 1,537,113 + +0.5 1,537,113 + +0.4 1,537,122 - +1.9 1,537,634 + Pf6N2E2_1302 0.38 -1.2 1,537,634 + Pf6N2E2_1302 0.38 -1.2 1,537,635 - Pf6N2E2_1302 0.38 -0.2 1,537,635 - Pf6N2E2_1302 0.38 +0.1 1,537,856 + Pf6N2E2_1302 0.55 +1.4 1,537,856 + Pf6N2E2_1302 0.55 -1.3 1,537,856 + Pf6N2E2_1302 0.55 +0.6 1,537,856 + Pf6N2E2_1302 0.55 +0.1 1,537,856 + Pf6N2E2_1302 0.55 -0.8 1,537,857 - Pf6N2E2_1302 0.55 -0.0
Or see this region's nucleotide sequence