Experiment: Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose with 10 mg/L CCCP; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20410 and IAI47_20415 overlap by 4 nucleotides IAI47_20415 and IAI47_20420 overlap by 7 nucleotides IAI47_20420 and IAI47_20425 are separated by 63 nucleotides
IAI47_20410: IAI47_20410 - two-component system sensor histidine kinase DcuS, at 322,172 to 323,806
_20410
IAI47_20415: IAI47_20415 - two-component system response regulator DcuR, at 323,803 to 324,522
_20415
IAI47_20420: IAI47_20420 - FAD:protein FMN transferase, at 324,516 to 325,478
_20420
IAI47_20425: IAI47_20425 - flavocytochrome c, at 325,542 to 328,322
_20425
Position (kb) 324
325
326 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 323.564 kb on + strand, within IAI47_20410 at 323.564 kb on + strand, within IAI47_20410 at 323.564 kb on + strand, within IAI47_20410 at 323.751 kb on + strand at 323.752 kb on - strand at 323.752 kb on - strand at 323.752 kb on - strand at 323.752 kb on - strand at 323.752 kb on - strand at 323.755 kb on + strand at 323.816 kb on + strand at 323.816 kb on + strand at 323.816 kb on + strand at 323.816 kb on + strand at 323.817 kb on - strand at 323.860 kb on + strand at 323.860 kb on + strand at 323.860 kb on + strand at 323.861 kb on - strand at 323.862 kb on + strand at 323.909 kb on + strand, within IAI47_20415 at 323.957 kb on + strand, within IAI47_20415 at 323.974 kb on + strand, within IAI47_20415 at 323.974 kb on + strand, within IAI47_20415 at 323.974 kb on + strand, within IAI47_20415 at 323.974 kb on + strand, within IAI47_20415 at 323.974 kb on + strand, within IAI47_20415 at 323.974 kb on + strand, within IAI47_20415 at 323.974 kb on + strand, within IAI47_20415 at 323.975 kb on - strand, within IAI47_20415 at 323.975 kb on - strand, within IAI47_20415 at 323.975 kb on - strand, within IAI47_20415 at 323.975 kb on - strand, within IAI47_20415 at 323.975 kb on - strand, within IAI47_20415 at 323.975 kb on - strand, within IAI47_20415 at 323.975 kb on - strand, within IAI47_20415 at 323.975 kb on - strand, within IAI47_20415 at 323.975 kb on - strand, within IAI47_20415 at 323.975 kb on - strand, within IAI47_20415 at 323.975 kb on - strand, within IAI47_20415 at 323.976 kb on + strand, within IAI47_20415 at 323.976 kb on + strand, within IAI47_20415 at 323.976 kb on + strand, within IAI47_20415 at 323.976 kb on + strand, within IAI47_20415 at 323.977 kb on - strand, within IAI47_20415 at 323.977 kb on - strand, within IAI47_20415 at 323.977 kb on - strand, within IAI47_20415 at 323.977 kb on - strand, within IAI47_20415 at 323.977 kb on - strand, within IAI47_20415 at 324.045 kb on + strand, within IAI47_20415 at 324.046 kb on - strand, within IAI47_20415 at 324.046 kb on - strand, within IAI47_20415 at 324.094 kb on + strand, within IAI47_20415 at 324.095 kb on - strand, within IAI47_20415 at 324.095 kb on - strand, within IAI47_20415 at 324.095 kb on - strand, within IAI47_20415 at 324.095 kb on - strand, within IAI47_20415 at 324.095 kb on - strand, within IAI47_20415 at 324.101 kb on + strand, within IAI47_20415 at 324.102 kb on - strand, within IAI47_20415 at 324.109 kb on + strand, within IAI47_20415 at 324.109 kb on + strand, within IAI47_20415 at 324.109 kb on + strand, within IAI47_20415 at 324.110 kb on - strand, within IAI47_20415 at 324.110 kb on - strand, within IAI47_20415 at 324.186 kb on + strand, within IAI47_20415 at 324.199 kb on + strand, within IAI47_20415 at 324.199 kb on + strand, within IAI47_20415 at 324.200 kb on - strand, within IAI47_20415 at 324.200 kb on - strand, within IAI47_20415 at 324.369 kb on + strand, within IAI47_20415 at 324.370 kb on - strand, within IAI47_20415 at 324.370 kb on - strand, within IAI47_20415 at 324.370 kb on - strand, within IAI47_20415 at 324.418 kb on - strand, within IAI47_20415 at 324.421 kb on - strand, within IAI47_20415 at 324.436 kb on - strand, within IAI47_20415 at 324.436 kb on - strand, within IAI47_20415 at 324.442 kb on + strand, within IAI47_20415 at 324.442 kb on + strand, within IAI47_20415 at 324.442 kb on + strand, within IAI47_20415 at 324.442 kb on + strand, within IAI47_20415 at 324.443 kb on - strand, within IAI47_20415 at 324.443 kb on - strand, within IAI47_20415 at 324.467 kb on - strand at 324.490 kb on + strand at 324.500 kb on + strand at 324.501 kb on - strand at 324.511 kb on + strand at 324.511 kb on + strand at 324.511 kb on + strand at 324.511 kb on + strand at 324.512 kb on - strand at 324.512 kb on - strand at 324.546 kb on + strand at 324.626 kb on + strand, within IAI47_20420 at 324.626 kb on + strand, within IAI47_20420 at 324.627 kb on - strand, within IAI47_20420 at 324.660 kb on + strand, within IAI47_20420 at 324.660 kb on + strand, within IAI47_20420 at 324.660 kb on + strand, within IAI47_20420 at 324.661 kb on - strand, within IAI47_20420 at 324.661 kb on - strand, within IAI47_20420 at 324.661 kb on - strand, within IAI47_20420 at 324.667 kb on + strand, within IAI47_20420 at 324.667 kb on + strand, within IAI47_20420 at 324.667 kb on + strand, within IAI47_20420 at 324.668 kb on - strand, within IAI47_20420 at 324.682 kb on + strand, within IAI47_20420 at 324.682 kb on + strand, within IAI47_20420 at 324.682 kb on + strand, within IAI47_20420 at 324.682 kb on + strand, within IAI47_20420 at 324.683 kb on - strand, within IAI47_20420 at 324.683 kb on - strand, within IAI47_20420 at 324.683 kb on - strand, within IAI47_20420 at 324.683 kb on - strand, within IAI47_20420 at 324.811 kb on + strand, within IAI47_20420 at 324.811 kb on + strand, within IAI47_20420 at 324.811 kb on + strand, within IAI47_20420 at 324.812 kb on - strand, within IAI47_20420 at 324.956 kb on + strand, within IAI47_20420 at 324.957 kb on - strand, within IAI47_20420 at 324.972 kb on - strand, within IAI47_20420 at 324.972 kb on - strand, within IAI47_20420 at 324.999 kb on + strand, within IAI47_20420 at 324.999 kb on + strand, within IAI47_20420 at 325.000 kb on - strand, within IAI47_20420 at 325.000 kb on - strand, within IAI47_20420 at 325.000 kb on - strand, within IAI47_20420 at 325.002 kb on + strand, within IAI47_20420 at 325.002 kb on + strand, within IAI47_20420 at 325.002 kb on + strand, within IAI47_20420 at 325.002 kb on + strand, within IAI47_20420 at 325.003 kb on - strand, within IAI47_20420 at 325.003 kb on - strand, within IAI47_20420 at 325.003 kb on - strand, within IAI47_20420 at 325.003 kb on - strand, within IAI47_20420 at 325.003 kb on - strand, within IAI47_20420 at 325.003 kb on - strand, within IAI47_20420 at 325.003 kb on - strand, within IAI47_20420 at 325.003 kb on - strand, within IAI47_20420 at 325.003 kb on - strand, within IAI47_20420 at 325.003 kb on - strand, within IAI47_20420 at 325.013 kb on + strand, within IAI47_20420 at 325.013 kb on + strand, within IAI47_20420 at 325.014 kb on - strand, within IAI47_20420 at 325.015 kb on + strand, within IAI47_20420 at 325.015 kb on + strand, within IAI47_20420 at 325.015 kb on + strand, within IAI47_20420 at 325.015 kb on + strand, within IAI47_20420 at 325.015 kb on + strand, within IAI47_20420 at 325.015 kb on + strand, within IAI47_20420 at 325.015 kb on + strand, within IAI47_20420 at 325.016 kb on - strand, within IAI47_20420 at 325.208 kb on + strand, within IAI47_20420 at 325.209 kb on - strand, within IAI47_20420 at 325.308 kb on - strand, within IAI47_20420 at 325.438 kb on - strand at 325.450 kb on + strand at 325.450 kb on + strand at 325.450 kb on + strand at 325.450 kb on + strand at 325.450 kb on + strand at 325.451 kb on - strand at 325.451 kb on - strand at 325.451 kb on - strand at 325.456 kb on + strand at 325.456 kb on + strand at 325.456 kb on + strand at 325.456 kb on + strand at 325.457 kb on - strand at 325.457 kb on - strand at 325.457 kb on - strand at 325.457 kb on - strand at 325.457 kb on - strand at 325.457 kb on - strand at 325.457 kb on - strand at 325.473 kb on + strand at 325.473 kb on + strand at 325.473 kb on + strand at 325.474 kb on - strand at 325.474 kb on - strand at 325.474 kb on - strand at 325.478 kb on + strand at 325.479 kb on - strand at 325.479 kb on - strand at 325.479 kb on - strand at 325.497 kb on + strand at 325.498 kb on - strand at 325.555 kb on + strand at 325.556 kb on - strand at 325.598 kb on + strand at 325.599 kb on - strand at 325.599 kb on - strand at 325.599 kb on - strand at 325.599 kb on - strand at 325.599 kb on - strand at 325.599 kb on - strand at 325.599 kb on - strand at 325.599 kb on - strand at 325.709 kb on + strand at 325.710 kb on - strand at 325.721 kb on + strand at 325.721 kb on + strand at 325.722 kb on - strand at 325.771 kb on + strand at 325.838 kb on + strand, within IAI47_20425 at 325.838 kb on + strand, within IAI47_20425 at 325.838 kb on + strand, within IAI47_20425 at 325.838 kb on + strand, within IAI47_20425 at 325.839 kb on - strand, within IAI47_20425 at 325.839 kb on - strand, within IAI47_20425 at 325.839 kb on - strand, within IAI47_20425 at 325.839 kb on - strand, within IAI47_20425 at 325.855 kb on + strand, within IAI47_20425 at 325.957 kb on + strand, within IAI47_20425 at 325.957 kb on + strand, within IAI47_20425 at 325.957 kb on + strand, within IAI47_20425 at 325.957 kb on + strand, within IAI47_20425 at 325.958 kb on - strand, within IAI47_20425 at 325.958 kb on - strand, within IAI47_20425 at 325.958 kb on - strand, within IAI47_20425 at 325.958 kb on - strand, within IAI47_20425 at 325.958 kb on - strand, within IAI47_20425 at 325.958 kb on - strand, within IAI47_20425 at 325.958 kb on - strand, within IAI47_20425 at 325.958 kb on - strand, within IAI47_20425 at 325.958 kb on - strand, within IAI47_20425 at 325.958 kb on - strand, within IAI47_20425 at 326.001 kb on + strand, within IAI47_20425 at 326.001 kb on + strand, within IAI47_20425 at 326.001 kb on + strand, within IAI47_20425 at 326.001 kb on + strand, within IAI47_20425 at 326.001 kb on + strand, within IAI47_20425 at 326.074 kb on + strand, within IAI47_20425 at 326.075 kb on - strand, within IAI47_20425 at 326.075 kb on - strand, within IAI47_20425 at 326.140 kb on + strand, within IAI47_20425 at 326.140 kb on + strand, within IAI47_20425 at 326.140 kb on + strand, within IAI47_20425 at 326.140 kb on + strand, within IAI47_20425 at 326.140 kb on + strand, within IAI47_20425 at 326.141 kb on - strand, within IAI47_20425 at 326.186 kb on + strand, within IAI47_20425 at 326.246 kb on + strand, within IAI47_20425 at 326.246 kb on + strand, within IAI47_20425 at 326.246 kb on + strand, within IAI47_20425 at 326.247 kb on - strand, within IAI47_20425 at 326.247 kb on - strand, within IAI47_20425 at 326.285 kb on - strand, within IAI47_20425 at 326.285 kb on - strand, within IAI47_20425 at 326.356 kb on - strand, within IAI47_20425 at 326.368 kb on + strand, within IAI47_20425 at 326.369 kb on - strand, within IAI47_20425 at 326.466 kb on - strand, within IAI47_20425 at 326.472 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose with 10 mg/L CCCP; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 323,564 + IAI47_20410 0.85 +0.7 323,564 + IAI47_20410 0.85 +2.2 323,564 + IAI47_20410 0.85 +0.2 323,751 + -0.2 323,752 - -0.2 323,752 - +0.3 323,752 - +1.3 323,752 - +0.8 323,752 - -1.6 323,755 + +2.2 323,816 + +0.3 323,816 + +0.0 323,816 + +1.4 323,816 + +0.3 323,817 - +2.2 323,860 + -0.1 323,860 + -0.4 323,860 + -0.3 323,861 - +0.9 323,862 + +1.2 323,909 + IAI47_20415 0.15 +2.2 323,957 + IAI47_20415 0.21 +0.9 323,974 + IAI47_20415 0.24 +0.2 323,974 + IAI47_20415 0.24 -0.2 323,974 + IAI47_20415 0.24 -0.2 323,974 + IAI47_20415 0.24 +0.2 323,974 + IAI47_20415 0.24 +1.2 323,974 + IAI47_20415 0.24 +1.0 323,974 + IAI47_20415 0.24 +1.5 323,975 - IAI47_20415 0.24 +0.0 323,975 - IAI47_20415 0.24 +0.9 323,975 - IAI47_20415 0.24 +1.1 323,975 - IAI47_20415 0.24 +0.0 323,975 - IAI47_20415 0.24 -0.0 323,975 - IAI47_20415 0.24 -0.8 323,975 - IAI47_20415 0.24 +0.9 323,975 - IAI47_20415 0.24 +0.8 323,975 - IAI47_20415 0.24 +0.4 323,975 - IAI47_20415 0.24 -0.4 323,975 - IAI47_20415 0.24 -0.7 323,976 + IAI47_20415 0.24 +0.6 323,976 + IAI47_20415 0.24 +1.6 323,976 + IAI47_20415 0.24 +1.4 323,976 + IAI47_20415 0.24 +0.6 323,977 - IAI47_20415 0.24 -0.3 323,977 - IAI47_20415 0.24 -0.7 323,977 - IAI47_20415 0.24 -1.5 323,977 - IAI47_20415 0.24 +1.6 323,977 - IAI47_20415 0.24 -0.2 324,045 + IAI47_20415 0.34 +0.3 324,046 - IAI47_20415 0.34 -0.7 324,046 - IAI47_20415 0.34 -0.2 324,094 + IAI47_20415 0.40 -0.6 324,095 - IAI47_20415 0.41 -0.4 324,095 - IAI47_20415 0.41 -0.7 324,095 - IAI47_20415 0.41 +0.7 324,095 - IAI47_20415 0.41 +0.3 324,095 - IAI47_20415 0.41 -0.2 324,101 + IAI47_20415 0.41 +0.4 324,102 - IAI47_20415 0.42 +0.6 324,109 + IAI47_20415 0.42 +1.3 324,109 + IAI47_20415 0.42 -0.2 324,109 + IAI47_20415 0.42 -0.2 324,110 - IAI47_20415 0.43 +1.7 324,110 - IAI47_20415 0.43 -1.5 324,186 + IAI47_20415 0.53 +2.2 324,199 + IAI47_20415 0.55 -0.4 324,199 + IAI47_20415 0.55 -0.4 324,200 - IAI47_20415 0.55 +0.5 324,200 - IAI47_20415 0.55 +0.9 324,369 + IAI47_20415 0.79 +0.9 324,370 - IAI47_20415 0.79 +1.2 324,370 - IAI47_20415 0.79 -0.8 324,370 - IAI47_20415 0.79 +0.2 324,418 - IAI47_20415 0.85 +0.3 324,421 - IAI47_20415 0.86 -0.2 324,436 - IAI47_20415 0.88 -0.6 324,436 - IAI47_20415 0.88 +1.2 324,442 + IAI47_20415 0.89 +1.9 324,442 + IAI47_20415 0.89 +0.8 324,442 + IAI47_20415 0.89 +0.2 324,442 + IAI47_20415 0.89 -0.9 324,443 - IAI47_20415 0.89 +0.6 324,443 - IAI47_20415 0.89 -1.5 324,467 - +1.6 324,490 + +0.0 324,500 + +0.6 324,501 - -1.0 324,511 + -0.3 324,511 + -0.1 324,511 + +1.4 324,511 + -0.1 324,512 - -0.6 324,512 - -1.3 324,546 + +1.3 324,626 + IAI47_20420 0.11 -0.8 324,626 + IAI47_20420 0.11 -0.7 324,627 - IAI47_20420 0.12 +0.0 324,660 + IAI47_20420 0.15 +0.3 324,660 + IAI47_20420 0.15 -1.7 324,660 + IAI47_20420 0.15 +0.9 324,661 - IAI47_20420 0.15 +1.7 324,661 - IAI47_20420 0.15 +0.7 324,661 - IAI47_20420 0.15 -1.1 324,667 + IAI47_20420 0.16 +1.2 324,667 + IAI47_20420 0.16 -2.0 324,667 + IAI47_20420 0.16 -1.1 324,668 - IAI47_20420 0.16 -0.1 324,682 + IAI47_20420 0.17 +0.0 324,682 + IAI47_20420 0.17 +0.7 324,682 + IAI47_20420 0.17 +0.7 324,682 + IAI47_20420 0.17 +0.4 324,683 - IAI47_20420 0.17 +0.2 324,683 - IAI47_20420 0.17 -0.7 324,683 - IAI47_20420 0.17 +0.4 324,683 - IAI47_20420 0.17 +0.9 324,811 + IAI47_20420 0.31 -0.1 324,811 + IAI47_20420 0.31 -0.8 324,811 + IAI47_20420 0.31 -0.4 324,812 - IAI47_20420 0.31 +1.7 324,956 + IAI47_20420 0.46 +0.6 324,957 - IAI47_20420 0.46 +1.4 324,972 - IAI47_20420 0.47 +1.5 324,972 - IAI47_20420 0.47 +0.8 324,999 + IAI47_20420 0.50 +3.7 324,999 + IAI47_20420 0.50 +3.1 325,000 - IAI47_20420 0.50 +0.3 325,000 - IAI47_20420 0.50 +1.2 325,000 - IAI47_20420 0.50 +0.9 325,002 + IAI47_20420 0.50 +2.2 325,002 + IAI47_20420 0.50 -0.3 325,002 + IAI47_20420 0.50 +0.7 325,002 + IAI47_20420 0.50 -0.2 325,003 - IAI47_20420 0.51 +3.1 325,003 - IAI47_20420 0.51 +0.2 325,003 - IAI47_20420 0.51 +2.2 325,003 - IAI47_20420 0.51 +1.2 325,003 - IAI47_20420 0.51 +1.7 325,003 - IAI47_20420 0.51 +0.6 325,003 - IAI47_20420 0.51 +1.8 325,003 - IAI47_20420 0.51 +0.0 325,003 - IAI47_20420 0.51 -1.0 325,003 - IAI47_20420 0.51 +0.3 325,013 + IAI47_20420 0.52 +0.7 325,013 + IAI47_20420 0.52 -1.1 325,014 - IAI47_20420 0.52 +1.7 325,015 + IAI47_20420 0.52 +1.0 325,015 + IAI47_20420 0.52 +1.3 325,015 + IAI47_20420 0.52 -0.4 325,015 + IAI47_20420 0.52 +0.4 325,015 + IAI47_20420 0.52 -1.0 325,015 + IAI47_20420 0.52 -0.4 325,015 + IAI47_20420 0.52 -0.7 325,016 - IAI47_20420 0.52 +2.2 325,208 + IAI47_20420 0.72 +0.6 325,209 - IAI47_20420 0.72 +1.6 325,308 - IAI47_20420 0.82 +2.2 325,438 - -1.4 325,450 + -0.2 325,450 + +0.4 325,450 + -0.3 325,450 + +2.5 325,450 + +0.9 325,451 - -1.0 325,451 - -0.8 325,451 - +1.7 325,456 + -0.4 325,456 + +1.4 325,456 + -0.4 325,456 + +0.6 325,457 - -0.8 325,457 - -0.1 325,457 - -0.5 325,457 - +0.7 325,457 - -0.2 325,457 - +1.7 325,457 - -0.3 325,473 + +0.2 325,473 + +3.2 325,473 + +0.8 325,474 - +1.4 325,474 - +0.8 325,474 - +1.4 325,478 + -1.7 325,479 - -0.2 325,479 - +1.7 325,479 - +0.3 325,497 + -0.4 325,498 - -0.8 325,555 + -1.0 325,556 - +1.3 325,598 + +0.7 325,599 - -0.4 325,599 - +0.7 325,599 - +1.6 325,599 - +0.7 325,599 - +0.3 325,599 - +0.3 325,599 - +0.6 325,599 - -1.0 325,709 + -0.7 325,710 - +2.2 325,721 + -0.3 325,721 + -0.7 325,722 - +0.0 325,771 + +1.9 325,838 + IAI47_20425 0.11 -1.5 325,838 + IAI47_20425 0.11 -0.9 325,838 + IAI47_20425 0.11 -0.6 325,838 + IAI47_20425 0.11 -0.4 325,839 - IAI47_20425 0.11 +0.2 325,839 - IAI47_20425 0.11 +0.9 325,839 - IAI47_20425 0.11 +0.3 325,839 - IAI47_20425 0.11 -0.9 325,855 + IAI47_20425 0.11 +1.7 325,957 + IAI47_20425 0.15 -0.8 325,957 + IAI47_20425 0.15 +0.9 325,957 + IAI47_20425 0.15 +0.7 325,957 + IAI47_20425 0.15 -0.2 325,958 - IAI47_20425 0.15 -0.3 325,958 - IAI47_20425 0.15 +1.8 325,958 - IAI47_20425 0.15 -0.4 325,958 - IAI47_20425 0.15 +2.0 325,958 - IAI47_20425 0.15 +1.1 325,958 - IAI47_20425 0.15 -0.5 325,958 - IAI47_20425 0.15 -1.0 325,958 - IAI47_20425 0.15 +1.1 325,958 - IAI47_20425 0.15 +0.3 325,958 - IAI47_20425 0.15 -0.1 326,001 + IAI47_20425 0.17 -1.9 326,001 + IAI47_20425 0.17 -3.4 326,001 + IAI47_20425 0.17 -0.6 326,001 + IAI47_20425 0.17 +0.5 326,001 + IAI47_20425 0.17 +0.3 326,074 + IAI47_20425 0.19 -0.1 326,075 - IAI47_20425 0.19 +2.2 326,075 - IAI47_20425 0.19 +0.6 326,140 + IAI47_20425 0.22 -0.3 326,140 + IAI47_20425 0.22 -1.3 326,140 + IAI47_20425 0.22 -0.4 326,140 + IAI47_20425 0.22 -1.0 326,140 + IAI47_20425 0.22 +1.4 326,141 - IAI47_20425 0.22 -0.0 326,186 + IAI47_20425 0.23 +0.6 326,246 + IAI47_20425 0.25 -1.3 326,246 + IAI47_20425 0.25 +0.3 326,246 + IAI47_20425 0.25 +3.2 326,247 - IAI47_20425 0.25 +0.9 326,247 - IAI47_20425 0.25 +0.3 326,285 - IAI47_20425 0.27 +1.6 326,285 - IAI47_20425 0.27 -0.3 326,356 - IAI47_20425 0.29 -0.9 326,368 + IAI47_20425 0.30 +0.6 326,369 - IAI47_20425 0.30 +0.9 326,466 - IAI47_20425 0.33 +0.9 326,472 + +1.4
Or see this region's nucleotide sequence