Experiment: Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose with 10 mg/L CCCP; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20000 and IAI47_20005 are separated by 48 nucleotides IAI47_20005 and IAI47_20010 are separated by 444 nucleotides IAI47_20010 and IAI47_20015 are separated by 417 nucleotides
IAI47_20000: IAI47_20000 - PTS sugar transporter subunit IIB, at 232,972 to 233,244
_20000
IAI47_20005: IAI47_20005 - PTS sugar transporter subunit IIA, at 233,293 to 233,742
_20005
IAI47_20010: IAI47_20010 - GntR family transcriptional regulator, at 234,187 to 234,921
_20010
IAI47_20015: IAI47_20015 - MsnO8 family LLM class oxidoreductase, at 235,339 to 236,337
_20015
Position (kb) 234
235 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 233.236 kb on - strand at 233.244 kb on + strand at 233.245 kb on - strand at 233.315 kb on - strand at 233.328 kb on + strand at 233.409 kb on + strand, within IAI47_20005 at 233.410 kb on - strand, within IAI47_20005 at 233.410 kb on - strand, within IAI47_20005 at 233.410 kb on - strand, within IAI47_20005 at 233.434 kb on - strand, within IAI47_20005 at 233.434 kb on - strand, within IAI47_20005 at 233.434 kb on - strand, within IAI47_20005 at 233.615 kb on + strand, within IAI47_20005 at 233.615 kb on + strand, within IAI47_20005 at 233.615 kb on + strand, within IAI47_20005 at 233.615 kb on + strand, within IAI47_20005 at 233.616 kb on - strand, within IAI47_20005 at 233.616 kb on - strand, within IAI47_20005 at 233.727 kb on + strand at 233.727 kb on + strand at 233.728 kb on - strand at 233.728 kb on - strand at 233.728 kb on - strand at 233.728 kb on - strand at 233.771 kb on + strand at 233.771 kb on + strand at 233.771 kb on + strand at 233.772 kb on - strand at 233.772 kb on - strand at 233.772 kb on - strand at 233.772 kb on - strand at 233.775 kb on + strand at 233.775 kb on + strand at 233.775 kb on + strand at 233.775 kb on + strand at 233.775 kb on + strand at 233.776 kb on - strand at 233.776 kb on - strand at 233.776 kb on - strand at 233.878 kb on - strand at 233.878 kb on - strand at 233.891 kb on - strand at 233.921 kb on - strand at 233.932 kb on - strand at 233.965 kb on - strand at 233.971 kb on + strand at 233.981 kb on + strand at 233.982 kb on - strand at 234.010 kb on + strand at 234.025 kb on + strand at 234.026 kb on - strand at 234.026 kb on - strand at 234.026 kb on - strand at 234.026 kb on - strand at 234.086 kb on - strand at 234.086 kb on - strand at 234.086 kb on - strand at 234.106 kb on - strand at 234.106 kb on - strand at 234.134 kb on + strand at 234.134 kb on + strand at 234.134 kb on + strand at 234.229 kb on + strand at 234.229 kb on + strand at 234.229 kb on + strand at 234.229 kb on + strand at 234.229 kb on + strand at 234.229 kb on + strand at 234.230 kb on - strand at 234.230 kb on - strand at 234.230 kb on - strand at 234.230 kb on - strand at 234.241 kb on - strand at 234.241 kb on - strand at 234.247 kb on - strand at 234.290 kb on + strand, within IAI47_20010 at 234.291 kb on - strand, within IAI47_20010 at 234.291 kb on - strand, within IAI47_20010 at 234.315 kb on + strand, within IAI47_20010 at 234.316 kb on - strand, within IAI47_20010 at 234.345 kb on + strand, within IAI47_20010 at 234.345 kb on + strand, within IAI47_20010 at 234.345 kb on + strand, within IAI47_20010 at 234.345 kb on + strand, within IAI47_20010 at 234.345 kb on + strand, within IAI47_20010 at 234.345 kb on + strand, within IAI47_20010 at 234.345 kb on + strand, within IAI47_20010 at 234.346 kb on - strand, within IAI47_20010 at 234.346 kb on - strand, within IAI47_20010 at 234.346 kb on - strand at 234.346 kb on - strand, within IAI47_20010 at 234.346 kb on - strand, within IAI47_20010 at 234.353 kb on + strand, within IAI47_20010 at 234.368 kb on + strand, within IAI47_20010 at 234.368 kb on + strand, within IAI47_20010 at 234.368 kb on + strand, within IAI47_20010 at 234.369 kb on - strand, within IAI47_20010 at 234.369 kb on - strand, within IAI47_20010 at 234.371 kb on - strand, within IAI47_20010 at 234.378 kb on - strand, within IAI47_20010 at 234.413 kb on + strand, within IAI47_20010 at 234.413 kb on + strand, within IAI47_20010 at 234.414 kb on - strand, within IAI47_20010 at 234.414 kb on - strand, within IAI47_20010 at 234.417 kb on + strand, within IAI47_20010 at 234.418 kb on - strand, within IAI47_20010 at 234.511 kb on - strand, within IAI47_20010 at 234.627 kb on + strand, within IAI47_20010 at 234.628 kb on - strand, within IAI47_20010 at 234.628 kb on - strand, within IAI47_20010 at 234.676 kb on + strand, within IAI47_20010 at 234.688 kb on + strand, within IAI47_20010 at 234.688 kb on + strand, within IAI47_20010 at 234.688 kb on + strand, within IAI47_20010 at 234.688 kb on + strand, within IAI47_20010 at 234.688 kb on + strand, within IAI47_20010 at 234.688 kb on + strand, within IAI47_20010 at 234.688 kb on + strand, within IAI47_20010 at 234.689 kb on - strand, within IAI47_20010 at 234.689 kb on - strand, within IAI47_20010 at 234.689 kb on - strand, within IAI47_20010 at 234.694 kb on + strand, within IAI47_20010 at 234.694 kb on + strand, within IAI47_20010 at 234.694 kb on + strand, within IAI47_20010 at 234.695 kb on - strand, within IAI47_20010 at 234.695 kb on - strand, within IAI47_20010 at 234.696 kb on + strand, within IAI47_20010 at 234.696 kb on + strand, within IAI47_20010 at 234.697 kb on - strand, within IAI47_20010 at 234.715 kb on - strand, within IAI47_20010 at 234.715 kb on - strand, within IAI47_20010 at 234.765 kb on + strand, within IAI47_20010 at 234.766 kb on - strand, within IAI47_20010 at 234.777 kb on + strand, within IAI47_20010 at 234.777 kb on + strand, within IAI47_20010 at 234.777 kb on + strand, within IAI47_20010 at 234.778 kb on - strand, within IAI47_20010 at 234.778 kb on - strand, within IAI47_20010 at 234.782 kb on + strand, within IAI47_20010 at 234.787 kb on - strand, within IAI47_20010 at 234.787 kb on - strand, within IAI47_20010 at 234.832 kb on + strand, within IAI47_20010 at 234.919 kb on + strand at 234.924 kb on - strand at 234.929 kb on - strand at 234.944 kb on + strand at 234.944 kb on + strand at 234.944 kb on + strand at 234.945 kb on - strand at 234.969 kb on + strand at 234.969 kb on + strand at 234.969 kb on + strand at 234.969 kb on + strand at 234.969 kb on + strand at 234.970 kb on - strand at 234.970 kb on - strand at 234.975 kb on + strand at 234.975 kb on + strand at 234.976 kb on - strand at 234.979 kb on + strand at 234.980 kb on - strand at 235.038 kb on - strand at 235.098 kb on + strand at 235.099 kb on - strand at 235.099 kb on - strand at 235.099 kb on - strand at 235.156 kb on + strand at 235.157 kb on - strand at 235.183 kb on + strand at 235.183 kb on + strand at 235.184 kb on - strand at 235.184 kb on - strand at 235.184 kb on - strand at 235.187 kb on - strand at 235.187 kb on - strand at 235.220 kb on + strand at 235.220 kb on + strand at 235.255 kb on - strand at 235.261 kb on + strand at 235.268 kb on + strand at 235.269 kb on + strand at 235.269 kb on + strand at 235.269 kb on + strand at 235.280 kb on - strand at 235.285 kb on + strand at 235.322 kb on + strand at 235.346 kb on - strand at 235.361 kb on + strand at 235.365 kb on + strand at 235.365 kb on + strand at 235.365 kb on + strand at 235.365 kb on + strand at 235.366 kb on - strand at 235.366 kb on - strand at 235.366 kb on - strand at 235.366 kb on - strand at 235.450 kb on + strand, within IAI47_20015 at 235.450 kb on + strand, within IAI47_20015 at 235.517 kb on + strand, within IAI47_20015 at 235.517 kb on + strand, within IAI47_20015 at 235.518 kb on - strand, within IAI47_20015 at 235.518 kb on - strand, within IAI47_20015 at 235.518 kb on - strand, within IAI47_20015 at 235.518 kb on - strand, within IAI47_20015 at 235.548 kb on + strand, within IAI47_20015 at 235.548 kb on + strand, within IAI47_20015 at 235.585 kb on + strand, within IAI47_20015 at 235.585 kb on + strand, within IAI47_20015 at 235.585 kb on + strand, within IAI47_20015 at 235.586 kb on - strand, within IAI47_20015 at 235.596 kb on + strand, within IAI47_20015 at 235.596 kb on + strand, within IAI47_20015 at 235.596 kb on + strand, within IAI47_20015 at 235.596 kb on + strand, within IAI47_20015 at 235.597 kb on - strand, within IAI47_20015 at 235.597 kb on - strand, within IAI47_20015 at 235.597 kb on - strand, within IAI47_20015 at 235.597 kb on - strand, within IAI47_20015 at 235.597 kb on - strand, within IAI47_20015 at 235.644 kb on + strand, within IAI47_20015 at 235.644 kb on + strand, within IAI47_20015 at 235.659 kb on - strand, within IAI47_20015 at 235.659 kb on - strand, within IAI47_20015 at 235.680 kb on - strand, within IAI47_20015 at 235.741 kb on - strand, within IAI47_20015 at 235.741 kb on - strand, within IAI47_20015 at 235.761 kb on + strand, within IAI47_20015 at 235.762 kb on - strand, within IAI47_20015 at 235.787 kb on - strand, within IAI47_20015 at 235.806 kb on - strand, within IAI47_20015
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose with 10 mg/L CCCP; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 233,236 - +1.3 233,244 + +1.0 233,245 - +2.0 233,315 - -0.3 233,328 + +3.2 233,409 + IAI47_20005 0.26 +0.8 233,410 - IAI47_20005 0.26 +1.3 233,410 - IAI47_20005 0.26 -0.2 233,410 - IAI47_20005 0.26 -0.2 233,434 - IAI47_20005 0.31 +1.2 233,434 - IAI47_20005 0.31 +2.2 233,434 - IAI47_20005 0.31 +0.5 233,615 + IAI47_20005 0.72 +0.2 233,615 + IAI47_20005 0.72 +0.5 233,615 + IAI47_20005 0.72 -1.0 233,615 + IAI47_20005 0.72 -0.8 233,616 - IAI47_20005 0.72 -0.1 233,616 - IAI47_20005 0.72 +0.4 233,727 + -1.2 233,727 + -0.7 233,728 - +2.9 233,728 - +2.2 233,728 - +0.6 233,728 - +1.8 233,771 + -1.6 233,771 + +0.1 233,771 + -0.9 233,772 - +1.1 233,772 - +2.1 233,772 - +2.8 233,772 - -0.3 233,775 + +0.6 233,775 + +0.2 233,775 + -0.5 233,775 + -0.9 233,775 + -0.7 233,776 - +0.8 233,776 - +1.7 233,776 - +0.6 233,878 - +2.2 233,878 - +1.9 233,891 - +0.4 233,921 - +2.3 233,932 - +0.3 233,965 - -0.5 233,971 + +1.2 233,981 + -0.9 233,982 - +1.4 234,010 + +1.7 234,025 + -0.8 234,026 - +2.0 234,026 - +2.5 234,026 - +0.9 234,026 - +0.3 234,086 - -1.3 234,086 - +0.7 234,086 - +0.7 234,106 - +0.9 234,106 - +0.7 234,134 + +0.6 234,134 + +0.1 234,134 + -1.2 234,229 + +0.2 234,229 + +0.3 234,229 + -1.3 234,229 + -1.3 234,229 + +1.2 234,229 + -0.0 234,230 - -1.6 234,230 - +0.3 234,230 - +1.6 234,230 - +0.9 234,241 - +0.4 234,241 - +0.6 234,247 - +1.4 234,290 + IAI47_20010 0.14 -0.0 234,291 - IAI47_20010 0.14 +0.3 234,291 - IAI47_20010 0.14 -0.2 234,315 + IAI47_20010 0.17 +0.0 234,316 - IAI47_20010 0.18 -1.3 234,345 + IAI47_20010 0.21 -0.3 234,345 + IAI47_20010 0.21 +1.3 234,345 + IAI47_20010 0.21 +1.4 234,345 + IAI47_20010 0.21 +0.4 234,345 + IAI47_20010 0.21 +2.2 234,345 + IAI47_20010 0.21 +0.7 234,345 + IAI47_20010 0.21 +2.2 234,346 - IAI47_20010 0.22 -0.8 234,346 - IAI47_20010 0.22 +0.0 234,346 - +3.7 234,346 - IAI47_20010 0.22 -1.4 234,346 - IAI47_20010 0.22 -0.3 234,353 + IAI47_20010 0.23 -1.5 234,368 + IAI47_20010 0.25 +0.8 234,368 + IAI47_20010 0.25 +0.0 234,368 + IAI47_20010 0.25 -0.5 234,369 - IAI47_20010 0.25 -1.5 234,369 - IAI47_20010 0.25 -2.5 234,371 - IAI47_20010 0.25 +0.9 234,378 - IAI47_20010 0.26 +1.3 234,413 + IAI47_20010 0.31 -0.4 234,413 + IAI47_20010 0.31 +2.4 234,414 - IAI47_20010 0.31 -0.1 234,414 - IAI47_20010 0.31 -0.4 234,417 + IAI47_20010 0.31 +0.5 234,418 - IAI47_20010 0.31 -0.4 234,511 - IAI47_20010 0.44 -0.2 234,627 + IAI47_20010 0.60 +1.9 234,628 - IAI47_20010 0.60 +0.6 234,628 - IAI47_20010 0.60 -1.0 234,676 + IAI47_20010 0.67 +0.5 234,688 + IAI47_20010 0.68 -0.5 234,688 + IAI47_20010 0.68 +0.7 234,688 + IAI47_20010 0.68 +2.2 234,688 + IAI47_20010 0.68 +1.3 234,688 + IAI47_20010 0.68 +1.9 234,688 + IAI47_20010 0.68 +0.3 234,688 + IAI47_20010 0.68 +0.9 234,689 - IAI47_20010 0.68 +0.4 234,689 - IAI47_20010 0.68 -2.1 234,689 - IAI47_20010 0.68 +0.6 234,694 + IAI47_20010 0.69 +0.9 234,694 + IAI47_20010 0.69 +0.2 234,694 + IAI47_20010 0.69 +0.4 234,695 - IAI47_20010 0.69 +0.0 234,695 - IAI47_20010 0.69 +0.0 234,696 + IAI47_20010 0.69 -0.1 234,696 + IAI47_20010 0.69 +1.7 234,697 - IAI47_20010 0.69 +0.4 234,715 - IAI47_20010 0.72 +1.2 234,715 - IAI47_20010 0.72 -1.2 234,765 + IAI47_20010 0.79 -0.1 234,766 - IAI47_20010 0.79 -0.8 234,777 + IAI47_20010 0.80 +0.6 234,777 + IAI47_20010 0.80 +1.6 234,777 + IAI47_20010 0.80 +0.7 234,778 - IAI47_20010 0.80 -0.4 234,778 - IAI47_20010 0.80 -0.5 234,782 + IAI47_20010 0.81 +0.8 234,787 - IAI47_20010 0.82 -0.3 234,787 - IAI47_20010 0.82 -0.2 234,832 + IAI47_20010 0.88 -1.5 234,919 + +0.8 234,924 - -0.8 234,929 - +1.4 234,944 + +0.6 234,944 + +2.2 234,944 + +0.5 234,945 - -0.4 234,969 + -0.8 234,969 + -1.0 234,969 + +1.2 234,969 + +2.7 234,969 + +0.8 234,970 - -0.9 234,970 - -0.6 234,975 + -0.3 234,975 + -0.5 234,976 - -1.3 234,979 + -0.8 234,980 - -1.1 235,038 - +0.3 235,098 + +2.7 235,099 - -0.6 235,099 - +0.4 235,099 - -0.6 235,156 + +0.1 235,157 - +2.1 235,183 + +1.2 235,183 + -1.3 235,184 - +1.7 235,184 - +0.0 235,184 - -0.1 235,187 - +0.3 235,187 - +1.2 235,220 + +0.9 235,220 + -0.9 235,255 - -0.4 235,261 + +0.6 235,268 + +2.1 235,269 + +0.2 235,269 + +0.3 235,269 + -0.1 235,280 - -0.9 235,285 + +1.6 235,322 + +0.7 235,346 - -0.8 235,361 + +2.2 235,365 + +1.0 235,365 + +1.5 235,365 + -0.3 235,365 + -0.3 235,366 - +1.4 235,366 - +1.7 235,366 - +2.0 235,366 - -0.2 235,450 + IAI47_20015 0.11 +1.2 235,450 + IAI47_20015 0.11 +1.4 235,517 + IAI47_20015 0.18 -0.3 235,517 + IAI47_20015 0.18 +1.0 235,518 - IAI47_20015 0.18 +0.0 235,518 - IAI47_20015 0.18 +0.6 235,518 - IAI47_20015 0.18 -0.6 235,518 - IAI47_20015 0.18 +0.2 235,548 + IAI47_20015 0.21 +0.7 235,548 + IAI47_20015 0.21 +0.3 235,585 + IAI47_20015 0.25 +2.3 235,585 + IAI47_20015 0.25 +1.1 235,585 + IAI47_20015 0.25 -0.4 235,586 - IAI47_20015 0.25 +1.5 235,596 + IAI47_20015 0.26 -0.8 235,596 + IAI47_20015 0.26 +0.2 235,596 + IAI47_20015 0.26 -0.8 235,596 + IAI47_20015 0.26 -0.3 235,597 - IAI47_20015 0.26 +0.4 235,597 - IAI47_20015 0.26 +1.4 235,597 - IAI47_20015 0.26 +0.3 235,597 - IAI47_20015 0.26 +0.4 235,597 - IAI47_20015 0.26 +1.2 235,644 + IAI47_20015 0.31 +0.6 235,644 + IAI47_20015 0.31 -0.0 235,659 - IAI47_20015 0.32 -0.4 235,659 - IAI47_20015 0.32 +1.7 235,680 - IAI47_20015 0.34 +1.2 235,741 - IAI47_20015 0.40 +1.7 235,741 - IAI47_20015 0.40 -0.3 235,761 + IAI47_20015 0.42 -1.3 235,762 - IAI47_20015 0.42 +1.9 235,787 - IAI47_20015 0.45 -0.1 235,806 - IAI47_20015 0.47 +0.4
Or see this region's nucleotide sequence