Experiment: Varel_Bryant medium with Dimethyl Sulfoxide 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0096 and BT0097 are separated by 1 nucleotides BT0097 and BT0098 are separated by 4 nucleotides BT0098 and BT0099 are separated by 383 nucleotides BT0099 and BT0100 are separated by 19 nucleotides
BT0096: BT0096 - hypothetical protein (NCBI ptt file), at 88,735 to 89,415
BT0096
BT0097: BT0097 - conserved protein found in conjugate transposon (NCBI ptt file), at 89,417 to 89,854
BT0097
BT0098: BT0098 - conserved protein found in conjugate transposon (NCBI ptt file), at 89,859 to 90,620
BT0098
BT0099: BT0099 - hypothetical protein (NCBI ptt file), at 91,004 to 91,285
BT0099
BT0100: BT0100 - hypothetical protein (NCBI ptt file), at 91,305 to 91,742
BT0100
Position (kb) 89
90
91 Strain fitness (log2 ratio) -2
-1
0
1 at 89.381 kb on + strand at 89.505 kb on + strand, within BT0097 at 89.687 kb on + strand, within BT0097 at 89.854 kb on + strand at 89.868 kb on + strand at 89.916 kb on + strand at 90.298 kb on + strand, within BT0098 at 90.359 kb on + strand, within BT0098 at 90.514 kb on + strand, within BT0098 at 91.597 kb on + strand, within BT0100 at 91.598 kb on - strand, within BT0100 at 91.610 kb on + strand, within BT0100
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Dimethyl Sulfoxide 2 vol% remove 89,381 + -1.1 89,505 + BT0097 0.20 -2.4 89,687 + BT0097 0.62 -0.4 89,854 + -0.9 89,868 + +0.9 89,916 + -1.7 90,298 + BT0098 0.58 -0.1 90,359 + BT0098 0.66 -2.0 90,514 + BT0098 0.86 -1.4 91,597 + BT0100 0.67 +0.1 91,598 - BT0100 0.67 -1.6 91,610 + BT0100 0.70 -2.3
Or see this region's nucleotide sequence