Experiment: Varel_Bryant medium with Dimethyl Sulfoxide 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0042 and BT0043 are separated by 30 nucleotides BT0043 and BT0044 are separated by 10 nucleotides BT0044 and BT0045 overlap by 4 nucleotides
BT0042: BT0042 - conserved hypothetical protein (NCBI ptt file), at 39,215 to 40,375
BT0042
BT0043: BT0043 - putative glycosyltransferase (NCBI ptt file), at 40,406 to 40,582
BT0043
BT0044: BT0044 - putative glycosyltransferase (NCBI ptt file), at 40,593 to 41,537
BT0044
BT0045: BT0045 - putative polysaccharide biosynthesis protein (NCBI ptt file), at 41,534 to 42,700
BT0045
Position (kb) 40
41
42 Strain fitness (log2 ratio) -2
-1
0
1
2 at 39.637 kb on + strand, within BT0042 at 39.695 kb on + strand, within BT0042 at 39.763 kb on - strand, within BT0042 at 39.813 kb on + strand, within BT0042 at 40.165 kb on - strand, within BT0042 at 40.232 kb on - strand, within BT0042 at 40.282 kb on + strand at 40.306 kb on - strand at 40.333 kb on + strand at 40.435 kb on + strand, within BT0043 at 40.436 kb on - strand, within BT0043 at 40.442 kb on - strand, within BT0043 at 40.468 kb on + strand, within BT0043 at 40.468 kb on + strand, within BT0043 at 40.584 kb on - strand at 40.592 kb on - strand at 40.613 kb on - strand at 40.713 kb on - strand, within BT0044 at 40.775 kb on - strand, within BT0044 at 40.830 kb on - strand, within BT0044 at 40.830 kb on - strand, within BT0044 at 41.045 kb on + strand, within BT0044 at 41.076 kb on - strand at 41.167 kb on + strand, within BT0044 at 41.191 kb on - strand, within BT0044 at 41.202 kb on - strand, within BT0044 at 41.247 kb on - strand, within BT0044 at 41.395 kb on - strand, within BT0044 at 41.454 kb on + strand at 41.459 kb on - strand at 41.466 kb on + strand at 41.490 kb on + strand at 41.491 kb on - strand at 41.557 kb on + strand at 41.589 kb on + strand at 41.594 kb on - strand at 41.601 kb on - strand at 41.605 kb on - strand at 41.680 kb on - strand, within BT0045 at 41.680 kb on - strand, within BT0045 at 41.685 kb on - strand, within BT0045 at 41.692 kb on - strand, within BT0045 at 41.704 kb on - strand, within BT0045 at 41.738 kb on + strand, within BT0045 at 41.785 kb on + strand, within BT0045 at 41.836 kb on + strand, within BT0045 at 41.852 kb on + strand, within BT0045 at 41.870 kb on - strand, within BT0045 at 41.872 kb on - strand, within BT0045 at 41.879 kb on - strand, within BT0045 at 41.879 kb on - strand, within BT0045 at 41.921 kb on - strand, within BT0045 at 41.936 kb on - strand, within BT0045 at 41.943 kb on + strand, within BT0045 at 41.944 kb on - strand, within BT0045 at 41.944 kb on - strand, within BT0045 at 41.979 kb on - strand, within BT0045 at 41.984 kb on + strand, within BT0045 at 41.984 kb on + strand, within BT0045 at 41.984 kb on + strand, within BT0045 at 41.985 kb on - strand, within BT0045 at 41.985 kb on - strand, within BT0045 at 41.985 kb on - strand, within BT0045 at 41.999 kb on - strand, within BT0045 at 42.048 kb on - strand, within BT0045 at 42.058 kb on + strand, within BT0045 at 42.074 kb on + strand, within BT0045 at 42.172 kb on - strand, within BT0045 at 42.178 kb on - strand, within BT0045 at 42.197 kb on - strand, within BT0045 at 42.289 kb on - strand, within BT0045 at 42.345 kb on - strand, within BT0045 at 42.365 kb on + strand, within BT0045 at 42.365 kb on + strand, within BT0045 at 42.366 kb on - strand, within BT0045 at 42.367 kb on + strand, within BT0045 at 42.367 kb on + strand, within BT0045 at 42.368 kb on - strand, within BT0045 at 42.368 kb on - strand, within BT0045 at 42.370 kb on + strand, within BT0045 at 42.400 kb on + strand, within BT0045 at 42.503 kb on - strand, within BT0045 at 42.506 kb on + strand, within BT0045
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Dimethyl Sulfoxide 2 vol% remove 39,637 + BT0042 0.36 -0.1 39,695 + BT0042 0.41 -0.1 39,763 - BT0042 0.47 +0.2 39,813 + BT0042 0.52 -1.1 40,165 - BT0042 0.82 +0.5 40,232 - BT0042 0.88 +0.3 40,282 + +1.5 40,306 - -1.9 40,333 + -0.1 40,435 + BT0043 0.16 -0.9 40,436 - BT0043 0.17 -0.1 40,442 - BT0043 0.20 -1.0 40,468 + BT0043 0.35 -0.4 40,468 + BT0043 0.35 -0.3 40,584 - -1.1 40,592 - -0.9 40,613 - -0.1 40,713 - BT0044 0.13 -1.4 40,775 - BT0044 0.19 -1.4 40,830 - BT0044 0.25 -1.7 40,830 - BT0044 0.25 +1.9 41,045 + BT0044 0.48 +0.9 41,076 - +0.9 41,167 + BT0044 0.61 -2.7 41,191 - BT0044 0.63 -1.3 41,202 - BT0044 0.64 -2.2 41,247 - BT0044 0.69 -0.1 41,395 - BT0044 0.85 -1.1 41,454 + +0.3 41,459 - -0.1 41,466 + -0.1 41,490 + -2.6 41,491 - +0.9 41,557 + -1.4 41,589 + -0.1 41,594 - +2.2 41,601 - -1.2 41,605 - -0.1 41,680 - BT0045 0.13 -0.3 41,680 - BT0045 0.13 +0.3 41,685 - BT0045 0.13 -2.1 41,692 - BT0045 0.14 -0.9 41,704 - BT0045 0.15 -0.1 41,738 + BT0045 0.17 -0.8 41,785 + BT0045 0.22 +0.9 41,836 + BT0045 0.26 -0.3 41,852 + BT0045 0.27 +0.9 41,870 - BT0045 0.29 -0.2 41,872 - BT0045 0.29 +2.5 41,879 - BT0045 0.30 +1.5 41,879 - BT0045 0.30 -1.9 41,921 - BT0045 0.33 -0.7 41,936 - BT0045 0.34 -0.4 41,943 + BT0045 0.35 -0.1 41,944 - BT0045 0.35 +0.9 41,944 - BT0045 0.35 -1.2 41,979 - BT0045 0.38 -0.1 41,984 + BT0045 0.39 +0.5 41,984 + BT0045 0.39 +0.9 41,984 + BT0045 0.39 -0.2 41,985 - BT0045 0.39 +1.9 41,985 - BT0045 0.39 -1.7 41,985 - BT0045 0.39 -0.9 41,999 - BT0045 0.40 +0.3 42,048 - BT0045 0.44 -0.7 42,058 + BT0045 0.45 +0.9 42,074 + BT0045 0.46 -1.1 42,172 - BT0045 0.55 +0.2 42,178 - BT0045 0.55 +0.9 42,197 - BT0045 0.57 -0.9 42,289 - BT0045 0.65 -0.7 42,345 - BT0045 0.69 -2.1 42,365 + BT0045 0.71 -0.9 42,365 + BT0045 0.71 -0.7 42,366 - BT0045 0.71 -0.7 42,367 + BT0045 0.71 +0.3 42,367 + BT0045 0.71 +0.9 42,368 - BT0045 0.71 +0.6 42,368 - BT0045 0.71 -0.7 42,370 + BT0045 0.72 -1.1 42,400 + BT0045 0.74 +0.2 42,503 - BT0045 0.83 +1.9 42,506 + BT0045 0.83 -1.1
Or see this region's nucleotide sequence