Experiment: apoplastic; Pepper
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3988 and Psyr_3989 are separated by 296 nucleotides Psyr_3989 and Psyr_3990 are separated by 2 nucleotides
Psyr_3988: Psyr_3988 - conserved hypothetical protein, at 4,746,168 to 4,747,388
_3988
Psyr_3989: Psyr_3989 - hypothetical protein, at 4,747,685 to 4,748,905
_3989
Psyr_3990: Psyr_3990 - conserved hypothetical protein, at 4,748,908 to 4,749,918
_3990
Position (kb)
4747
4748
4749 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4746.748 kb on + strand, within Psyr_3988 at 4746.748 kb on + strand, within Psyr_3988 at 4746.749 kb on - strand, within Psyr_3988 at 4746.749 kb on - strand, within Psyr_3988 at 4746.772 kb on + strand, within Psyr_3988 at 4746.773 kb on - strand, within Psyr_3988 at 4746.869 kb on + strand, within Psyr_3988 at 4746.870 kb on - strand, within Psyr_3988 at 4746.964 kb on + strand, within Psyr_3988 at 4747.184 kb on - strand, within Psyr_3988 at 4747.184 kb on - strand, within Psyr_3988 at 4747.270 kb on + strand at 4747.270 kb on + strand at 4747.270 kb on + strand at 4747.271 kb on - strand at 4747.377 kb on - strand at 4747.377 kb on - strand at 4747.377 kb on - strand at 4747.487 kb on - strand at 4747.487 kb on - strand at 4747.509 kb on + strand at 4747.509 kb on + strand at 4747.509 kb on - strand at 4747.596 kb on + strand at 4747.596 kb on + strand at 4747.597 kb on - strand at 4747.597 kb on - strand at 4747.597 kb on - strand at 4747.599 kb on - strand at 4747.599 kb on - strand at 4747.615 kb on + strand at 4747.616 kb on - strand at 4747.617 kb on + strand at 4747.617 kb on + strand at 4747.617 kb on + strand at 4747.618 kb on - strand at 4747.618 kb on - strand at 4747.618 kb on - strand at 4747.618 kb on - strand at 4747.621 kb on + strand at 4747.630 kb on - strand at 4747.662 kb on - strand at 4747.662 kb on - strand at 4747.684 kb on + strand at 4747.684 kb on + strand at 4747.684 kb on + strand at 4747.684 kb on + strand at 4747.685 kb on - strand at 4747.685 kb on - strand at 4747.685 kb on - strand at 4747.738 kb on - strand at 4747.746 kb on + strand at 4747.751 kb on + strand at 4747.751 kb on + strand at 4747.752 kb on - strand at 4747.752 kb on - strand at 4747.752 kb on - strand at 4747.762 kb on + strand at 4747.762 kb on + strand at 4747.762 kb on + strand at 4747.762 kb on + strand at 4747.763 kb on - strand at 4747.763 kb on - strand at 4747.764 kb on + strand at 4747.765 kb on - strand at 4747.765 kb on - strand at 4747.765 kb on - strand at 4747.841 kb on + strand, within Psyr_3989 at 4747.903 kb on + strand, within Psyr_3989 at 4747.904 kb on - strand, within Psyr_3989 at 4747.904 kb on - strand, within Psyr_3989 at 4747.904 kb on - strand, within Psyr_3989 at 4747.912 kb on + strand, within Psyr_3989 at 4747.933 kb on + strand, within Psyr_3989 at 4747.934 kb on - strand, within Psyr_3989 at 4747.952 kb on + strand, within Psyr_3989 at 4747.952 kb on + strand, within Psyr_3989 at 4747.953 kb on - strand, within Psyr_3989 at 4747.953 kb on - strand, within Psyr_3989 at 4747.953 kb on - strand, within Psyr_3989 at 4747.953 kb on - strand, within Psyr_3989 at 4747.953 kb on - strand, within Psyr_3989 at 4747.953 kb on - strand, within Psyr_3989 at 4748.075 kb on + strand, within Psyr_3989 at 4748.075 kb on + strand, within Psyr_3989 at 4748.075 kb on + strand, within Psyr_3989 at 4748.075 kb on + strand, within Psyr_3989 at 4748.075 kb on + strand, within Psyr_3989 at 4748.076 kb on - strand, within Psyr_3989 at 4748.076 kb on - strand, within Psyr_3989 at 4748.076 kb on - strand, within Psyr_3989 at 4748.076 kb on - strand, within Psyr_3989 at 4748.076 kb on - strand, within Psyr_3989 at 4748.076 kb on - strand, within Psyr_3989 at 4748.149 kb on + strand, within Psyr_3989 at 4748.150 kb on - strand, within Psyr_3989 at 4748.233 kb on - strand, within Psyr_3989 at 4748.279 kb on - strand, within Psyr_3989 at 4748.279 kb on - strand, within Psyr_3989 at 4748.327 kb on - strand, within Psyr_3989 at 4748.331 kb on + strand, within Psyr_3989 at 4748.331 kb on + strand, within Psyr_3989 at 4748.331 kb on + strand, within Psyr_3989 at 4748.331 kb on + strand, within Psyr_3989 at 4748.331 kb on + strand, within Psyr_3989 at 4748.409 kb on + strand, within Psyr_3989 at 4748.409 kb on + strand, within Psyr_3989 at 4748.409 kb on + strand, within Psyr_3989 at 4748.409 kb on + strand, within Psyr_3989 at 4748.409 kb on + strand, within Psyr_3989 at 4748.409 kb on + strand, within Psyr_3989 at 4748.410 kb on - strand, within Psyr_3989 at 4748.410 kb on - strand, within Psyr_3989 at 4748.410 kb on - strand, within Psyr_3989 at 4748.429 kb on + strand, within Psyr_3989 at 4748.429 kb on + strand, within Psyr_3989 at 4748.430 kb on - strand, within Psyr_3989 at 4748.430 kb on - strand, within Psyr_3989 at 4748.430 kb on - strand, within Psyr_3989 at 4748.430 kb on - strand, within Psyr_3989 at 4748.430 kb on - strand, within Psyr_3989 at 4748.430 kb on - strand, within Psyr_3989 at 4748.431 kb on + strand, within Psyr_3989 at 4748.431 kb on + strand, within Psyr_3989 at 4748.432 kb on - strand, within Psyr_3989 at 4748.625 kb on + strand, within Psyr_3989 at 4748.638 kb on + strand, within Psyr_3989 at 4748.638 kb on + strand, within Psyr_3989 at 4748.644 kb on + strand, within Psyr_3989 at 4748.691 kb on + strand, within Psyr_3989 at 4748.691 kb on + strand, within Psyr_3989 at 4748.691 kb on + strand, within Psyr_3989 at 4748.691 kb on + strand, within Psyr_3989 at 4748.691 kb on + strand, within Psyr_3989 at 4748.692 kb on - strand, within Psyr_3989 at 4748.692 kb on - strand, within Psyr_3989 at 4748.692 kb on - strand, within Psyr_3989 at 4748.692 kb on - strand, within Psyr_3989 at 4748.692 kb on - strand, within Psyr_3989 at 4748.711 kb on + strand, within Psyr_3989 at 4748.711 kb on + strand, within Psyr_3989 at 4748.711 kb on + strand, within Psyr_3989 at 4748.711 kb on + strand, within Psyr_3989 at 4748.711 kb on + strand, within Psyr_3989 at 4748.711 kb on + strand, within Psyr_3989 at 4748.711 kb on + strand, within Psyr_3989 at 4748.712 kb on - strand, within Psyr_3989 at 4748.712 kb on - strand, within Psyr_3989 at 4748.712 kb on - strand, within Psyr_3989 at 4748.712 kb on - strand, within Psyr_3989 at 4748.712 kb on - strand, within Psyr_3989 at 4748.712 kb on - strand, within Psyr_3989 at 4748.712 kb on - strand, within Psyr_3989 at 4748.777 kb on - strand, within Psyr_3989 at 4748.777 kb on - strand, within Psyr_3989 at 4748.815 kb on - strand at 4748.836 kb on - strand at 4748.985 kb on + strand at 4748.986 kb on - strand at 4749.003 kb on + strand at 4749.003 kb on + strand at 4749.003 kb on + strand at 4749.004 kb on - strand at 4749.004 kb on - strand at 4749.004 kb on - strand at 4749.113 kb on + strand, within Psyr_3990 at 4749.113 kb on + strand, within Psyr_3990 at 4749.113 kb on + strand, within Psyr_3990 at 4749.113 kb on + strand, within Psyr_3990 at 4749.113 kb on + strand, within Psyr_3990 at 4749.114 kb on - strand, within Psyr_3990 at 4749.114 kb on - strand, within Psyr_3990 at 4749.114 kb on - strand, within Psyr_3990 at 4749.114 kb on - strand, within Psyr_3990 at 4749.114 kb on - strand, within Psyr_3990 at 4749.114 kb on - strand, within Psyr_3990 at 4749.114 kb on - strand, within Psyr_3990 at 4749.303 kb on + strand, within Psyr_3990 at 4749.304 kb on - strand, within Psyr_3990 at 4749.315 kb on - strand, within Psyr_3990 at 4749.315 kb on - strand, within Psyr_3990 at 4749.350 kb on + strand, within Psyr_3990 at 4749.350 kb on + strand, within Psyr_3990 at 4749.350 kb on + strand, within Psyr_3990 at 4749.350 kb on + strand, within Psyr_3990 at 4749.350 kb on + strand, within Psyr_3990 at 4749.350 kb on + strand, within Psyr_3990 at 4749.350 kb on + strand, within Psyr_3990 at 4749.350 kb on + strand, within Psyr_3990 at 4749.350 kb on + strand, within Psyr_3990 at 4749.350 kb on + strand, within Psyr_3990 at 4749.350 kb on + strand, within Psyr_3990 at 4749.351 kb on - strand, within Psyr_3990 at 4749.351 kb on - strand, within Psyr_3990 at 4749.351 kb on - strand, within Psyr_3990 at 4749.351 kb on - strand, within Psyr_3990 at 4749.351 kb on - strand, within Psyr_3990 at 4749.351 kb on - strand, within Psyr_3990 at 4749.351 kb on - strand, within Psyr_3990 at 4749.351 kb on - strand, within Psyr_3990 at 4749.351 kb on - strand, within Psyr_3990 at 4749.351 kb on - strand, within Psyr_3990 at 4749.351 kb on - strand, within Psyr_3990 at 4749.447 kb on + strand, within Psyr_3990 at 4749.447 kb on + strand, within Psyr_3990 at 4749.448 kb on - strand, within Psyr_3990 at 4749.457 kb on + strand, within Psyr_3990 at 4749.457 kb on + strand, within Psyr_3990 at 4749.457 kb on + strand, within Psyr_3990 at 4749.466 kb on + strand, within Psyr_3990 at 4749.467 kb on - strand, within Psyr_3990 at 4749.501 kb on + strand, within Psyr_3990 at 4749.502 kb on - strand, within Psyr_3990 at 4749.613 kb on + strand, within Psyr_3990 at 4749.613 kb on + strand, within Psyr_3990 at 4749.613 kb on + strand, within Psyr_3990 at 4749.613 kb on + strand, within Psyr_3990 at 4749.613 kb on + strand, within Psyr_3990 at 4749.613 kb on + strand, within Psyr_3990 at 4749.613 kb on + strand, within Psyr_3990 at 4749.614 kb on - strand, within Psyr_3990 at 4749.614 kb on - strand, within Psyr_3990 at 4749.614 kb on - strand, within Psyr_3990 at 4749.655 kb on + strand, within Psyr_3990 at 4749.656 kb on - strand, within Psyr_3990 at 4749.658 kb on - strand, within Psyr_3990 at 4749.803 kb on - strand, within Psyr_3990 at 4749.803 kb on - strand, within Psyr_3990 at 4749.803 kb on - strand, within Psyr_3990 at 4749.803 kb on - strand, within Psyr_3990 at 4749.803 kb on - strand, within Psyr_3990 at 4749.880 kb on + strand at 4749.880 kb on + strand at 4749.880 kb on + strand at 4749.881 kb on - strand at 4749.881 kb on - strand at 4749.884 kb on + strand at 4749.885 kb on - strand at 4749.885 kb on - strand at 4749.885 kb on - strand at 4749.885 kb on - strand at 4749.885 kb on - strand at 4749.885 kb on - strand at 4749.885 kb on - strand at 4749.895 kb on + strand at 4749.895 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction apoplastic; Pepper remove 4,746,748 + Psyr_3988 0.48 +1.0 4,746,748 + Psyr_3988 0.48 -1.8 4,746,749 - Psyr_3988 0.48 +0.9 4,746,749 - Psyr_3988 0.48 -2.4 4,746,772 + Psyr_3988 0.49 -0.8 4,746,773 - Psyr_3988 0.50 +1.7 4,746,869 + Psyr_3988 0.57 +2.3 4,746,870 - Psyr_3988 0.57 +1.6 4,746,964 + Psyr_3988 0.65 -0.6 4,747,184 - Psyr_3988 0.83 +0.4 4,747,184 - Psyr_3988 0.83 -2.6 4,747,270 + +0.8 4,747,270 + -1.5 4,747,270 + -3.4 4,747,271 - -0.7 4,747,377 - -0.9 4,747,377 - +1.1 4,747,377 - +1.8 4,747,487 - -2.6 4,747,487 - +0.5 4,747,509 + -0.1 4,747,509 + +0.9 4,747,509 - -0.2 4,747,596 + -1.9 4,747,596 + -1.6 4,747,597 - -0.2 4,747,597 - +0.6 4,747,597 - -0.6 4,747,599 - -0.5 4,747,599 - -1.5 4,747,615 + -1.8 4,747,616 - -2.1 4,747,617 + -1.8 4,747,617 + +1.5 4,747,617 + -1.6 4,747,618 - -1.6 4,747,618 - -0.4 4,747,618 - +1.3 4,747,618 - +1.3 4,747,621 + +0.1 4,747,630 - +0.5 4,747,662 - +0.1 4,747,662 - +0.6 4,747,684 + +0.4 4,747,684 + +0.6 4,747,684 + -1.9 4,747,684 + -0.2 4,747,685 - +0.8 4,747,685 - -2.8 4,747,685 - +2.9 4,747,738 - -1.5 4,747,746 + -1.7 4,747,751 + +0.4 4,747,751 + +0.1 4,747,752 - +2.0 4,747,752 - -2.2 4,747,752 - -2.9 4,747,762 + -0.8 4,747,762 + +1.5 4,747,762 + -0.4 4,747,762 + +0.4 4,747,763 - -0.3 4,747,763 - +0.7 4,747,764 + +0.8 4,747,765 - -0.0 4,747,765 - -1.6 4,747,765 - -0.5 4,747,841 + Psyr_3989 0.13 -2.7 4,747,903 + Psyr_3989 0.18 -0.9 4,747,904 - Psyr_3989 0.18 -3.0 4,747,904 - Psyr_3989 0.18 +0.9 4,747,904 - Psyr_3989 0.18 +1.2 4,747,912 + Psyr_3989 0.19 +0.6 4,747,933 + Psyr_3989 0.20 +1.2 4,747,934 - Psyr_3989 0.20 -0.6 4,747,952 + Psyr_3989 0.22 +2.4 4,747,952 + Psyr_3989 0.22 -0.9 4,747,953 - Psyr_3989 0.22 -0.6 4,747,953 - Psyr_3989 0.22 +1.2 4,747,953 - Psyr_3989 0.22 +0.9 4,747,953 - Psyr_3989 0.22 -0.4 4,747,953 - Psyr_3989 0.22 +2.6 4,747,953 - Psyr_3989 0.22 +0.2 4,748,075 + Psyr_3989 0.32 +1.1 4,748,075 + Psyr_3989 0.32 -0.6 4,748,075 + Psyr_3989 0.32 +0.6 4,748,075 + Psyr_3989 0.32 -1.8 4,748,075 + Psyr_3989 0.32 +1.7 4,748,076 - Psyr_3989 0.32 +1.6 4,748,076 - Psyr_3989 0.32 -2.1 4,748,076 - Psyr_3989 0.32 -3.3 4,748,076 - Psyr_3989 0.32 -2.4 4,748,076 - Psyr_3989 0.32 -1.8 4,748,076 - Psyr_3989 0.32 +1.5 4,748,149 + Psyr_3989 0.38 +1.3 4,748,150 - Psyr_3989 0.38 -1.4 4,748,233 - Psyr_3989 0.45 +1.4 4,748,279 - Psyr_3989 0.49 +0.8 4,748,279 - Psyr_3989 0.49 +1.9 4,748,327 - Psyr_3989 0.53 +0.3 4,748,331 + Psyr_3989 0.53 +1.9 4,748,331 + Psyr_3989 0.53 -0.2 4,748,331 + Psyr_3989 0.53 -3.7 4,748,331 + Psyr_3989 0.53 -3.4 4,748,331 + Psyr_3989 0.53 -0.3 4,748,409 + Psyr_3989 0.59 +0.5 4,748,409 + Psyr_3989 0.59 +1.2 4,748,409 + Psyr_3989 0.59 -0.4 4,748,409 + Psyr_3989 0.59 -0.8 4,748,409 + Psyr_3989 0.59 +0.9 4,748,409 + Psyr_3989 0.59 +2.0 4,748,410 - Psyr_3989 0.59 -0.1 4,748,410 - Psyr_3989 0.59 +0.3 4,748,410 - Psyr_3989 0.59 -0.2 4,748,429 + Psyr_3989 0.61 +1.2 4,748,429 + Psyr_3989 0.61 +1.7 4,748,430 - Psyr_3989 0.61 +0.7 4,748,430 - Psyr_3989 0.61 -2.3 4,748,430 - Psyr_3989 0.61 -0.9 4,748,430 - Psyr_3989 0.61 -1.9 4,748,430 - Psyr_3989 0.61 -1.4 4,748,430 - Psyr_3989 0.61 -2.8 4,748,431 + Psyr_3989 0.61 -2.4 4,748,431 + Psyr_3989 0.61 -1.2 4,748,432 - Psyr_3989 0.61 +0.6 4,748,625 + Psyr_3989 0.77 +0.4 4,748,638 + Psyr_3989 0.78 +0.6 4,748,638 + Psyr_3989 0.78 -1.2 4,748,644 + Psyr_3989 0.79 +0.4 4,748,691 + Psyr_3989 0.82 -1.1 4,748,691 + Psyr_3989 0.82 -1.3 4,748,691 + Psyr_3989 0.82 +1.1 4,748,691 + Psyr_3989 0.82 -0.5 4,748,691 + Psyr_3989 0.82 +2.0 4,748,692 - Psyr_3989 0.82 +1.2 4,748,692 - Psyr_3989 0.82 +0.8 4,748,692 - Psyr_3989 0.82 -1.8 4,748,692 - Psyr_3989 0.82 -1.2 4,748,692 - Psyr_3989 0.82 -2.6 4,748,711 + Psyr_3989 0.84 +0.3 4,748,711 + Psyr_3989 0.84 +0.1 4,748,711 + Psyr_3989 0.84 +1.4 4,748,711 + Psyr_3989 0.84 +0.9 4,748,711 + Psyr_3989 0.84 +3.2 4,748,711 + Psyr_3989 0.84 -1.9 4,748,711 + Psyr_3989 0.84 -3.5 4,748,712 - Psyr_3989 0.84 +0.7 4,748,712 - Psyr_3989 0.84 -3.0 4,748,712 - Psyr_3989 0.84 -0.0 4,748,712 - Psyr_3989 0.84 -2.1 4,748,712 - Psyr_3989 0.84 -2.7 4,748,712 - Psyr_3989 0.84 -0.2 4,748,712 - Psyr_3989 0.84 +0.1 4,748,777 - Psyr_3989 0.89 +1.4 4,748,777 - Psyr_3989 0.89 +1.0 4,748,815 - +0.2 4,748,836 - +0.7 4,748,985 + +0.2 4,748,986 - +1.6 4,749,003 + +1.4 4,749,003 + -1.2 4,749,003 + -2.9 4,749,004 - +0.2 4,749,004 - +0.4 4,749,004 - +1.8 4,749,113 + Psyr_3990 0.20 +2.2 4,749,113 + Psyr_3990 0.20 -0.9 4,749,113 + Psyr_3990 0.20 +1.1 4,749,113 + Psyr_3990 0.20 -0.9 4,749,113 + Psyr_3990 0.20 -0.1 4,749,114 - Psyr_3990 0.20 -0.5 4,749,114 - Psyr_3990 0.20 +0.8 4,749,114 - Psyr_3990 0.20 +1.1 4,749,114 - Psyr_3990 0.20 +0.8 4,749,114 - Psyr_3990 0.20 -1.6 4,749,114 - Psyr_3990 0.20 -2.5 4,749,114 - Psyr_3990 0.20 +1.0 4,749,303 + Psyr_3990 0.39 +0.8 4,749,304 - Psyr_3990 0.39 +0.2 4,749,315 - Psyr_3990 0.40 +0.3 4,749,315 - Psyr_3990 0.40 +1.3 4,749,350 + Psyr_3990 0.44 -0.9 4,749,350 + Psyr_3990 0.44 -1.2 4,749,350 + Psyr_3990 0.44 -1.6 4,749,350 + Psyr_3990 0.44 +0.2 4,749,350 + Psyr_3990 0.44 -1.4 4,749,350 + Psyr_3990 0.44 -1.9 4,749,350 + Psyr_3990 0.44 +0.2 4,749,350 + Psyr_3990 0.44 -0.6 4,749,350 + Psyr_3990 0.44 +2.6 4,749,350 + Psyr_3990 0.44 +0.9 4,749,350 + Psyr_3990 0.44 -1.3 4,749,351 - Psyr_3990 0.44 +0.5 4,749,351 - Psyr_3990 0.44 -1.2 4,749,351 - Psyr_3990 0.44 +0.1 4,749,351 - Psyr_3990 0.44 +1.4 4,749,351 - Psyr_3990 0.44 +1.5 4,749,351 - Psyr_3990 0.44 +1.0 4,749,351 - Psyr_3990 0.44 +0.5 4,749,351 - Psyr_3990 0.44 +0.6 4,749,351 - Psyr_3990 0.44 -2.1 4,749,351 - Psyr_3990 0.44 -0.4 4,749,351 - Psyr_3990 0.44 +0.8 4,749,447 + Psyr_3990 0.53 +0.1 4,749,447 + Psyr_3990 0.53 +1.1 4,749,448 - Psyr_3990 0.53 +0.4 4,749,457 + Psyr_3990 0.54 -0.9 4,749,457 + Psyr_3990 0.54 -1.8 4,749,457 + Psyr_3990 0.54 -2.6 4,749,466 + Psyr_3990 0.55 -1.4 4,749,467 - Psyr_3990 0.55 +0.8 4,749,501 + Psyr_3990 0.59 -1.6 4,749,502 - Psyr_3990 0.59 +0.4 4,749,613 + Psyr_3990 0.70 -1.9 4,749,613 + Psyr_3990 0.70 +0.6 4,749,613 + Psyr_3990 0.70 -0.9 4,749,613 + Psyr_3990 0.70 -0.2 4,749,613 + Psyr_3990 0.70 -0.1 4,749,613 + Psyr_3990 0.70 +1.5 4,749,613 + Psyr_3990 0.70 -2.6 4,749,614 - Psyr_3990 0.70 -0.7 4,749,614 - Psyr_3990 0.70 -0.1 4,749,614 - Psyr_3990 0.70 -0.5 4,749,655 + Psyr_3990 0.74 +1.3 4,749,656 - Psyr_3990 0.74 -0.4 4,749,658 - Psyr_3990 0.74 -2.3 4,749,803 - Psyr_3990 0.89 -0.3 4,749,803 - Psyr_3990 0.89 +0.1 4,749,803 - Psyr_3990 0.89 -0.5 4,749,803 - Psyr_3990 0.89 +1.8 4,749,803 - Psyr_3990 0.89 +0.5 4,749,880 + -0.3 4,749,880 + -0.0 4,749,880 + +0.2 4,749,881 - -0.7 4,749,881 - -1.7 4,749,884 + -3.7 4,749,885 - -0.6 4,749,885 - -1.2 4,749,885 - +0.7 4,749,885 - -1.4 4,749,885 - -1.4 4,749,885 - +0.8 4,749,885 - -2.0 4,749,895 + +1.9 4,749,895 + +2.3
Or see this region's nucleotide sequence