Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_612

Experiment: Nickel (II) chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_611 and Pf6N2E2_612 are separated by 12 nucleotidesPf6N2E2_612 and Pf6N2E2_613 are separated by 118 nucleotidesPf6N2E2_613 and Pf6N2E2_614 overlap by 14 nucleotides Pf6N2E2_611 - 2-dehydro-3-deoxy-L-arabinonate dehydratase (EC 4.2.1.43) (from data), at 694,827 to 695,819 _611 Pf6N2E2_612 - Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26) (from data), at 695,832 to 697,412 _612 Pf6N2E2_613 - lipoprotein, putative, at 697,531 to 697,869 _613 Pf6N2E2_614 - 3-carboxymuconate cyclase, at 697,856 to 699,031 _614 Position (kb) 695 696 697 698Strain fitness (log2 ratio) -1 0 1at 694.834 kb on + strandat 694.835 kb on - strandat 694.835 kb on - strandat 695.007 kb on + strand, within Pf6N2E2_611at 695.245 kb on - strand, within Pf6N2E2_611at 695.343 kb on + strand, within Pf6N2E2_611at 695.343 kb on + strandat 695.343 kb on + strand, within Pf6N2E2_611at 695.343 kb on + strand, within Pf6N2E2_611at 695.344 kb on - strand, within Pf6N2E2_611at 695.344 kb on - strand, within Pf6N2E2_611at 695.430 kb on + strand, within Pf6N2E2_611at 695.430 kb on + strand, within Pf6N2E2_611at 695.431 kb on - strand, within Pf6N2E2_611at 695.431 kb on - strand, within Pf6N2E2_611at 695.431 kb on - strand, within Pf6N2E2_611at 695.436 kb on + strand, within Pf6N2E2_611at 695.625 kb on + strand, within Pf6N2E2_611at 695.626 kb on - strand, within Pf6N2E2_611at 695.817 kb on + strandat 695.818 kb on - strandat 695.856 kb on + strandat 695.856 kb on + strandat 695.856 kb on + strandat 695.856 kb on + strandat 695.856 kb on + strandat 695.856 kb on + strandat 695.856 kb on + strandat 695.856 kb on + strandat 695.857 kb on - strandat 695.857 kb on - strandat 695.857 kb on - strandat 695.857 kb on - strandat 695.857 kb on - strandat 696.308 kb on + strand, within Pf6N2E2_612at 696.309 kb on - strand, within Pf6N2E2_612at 696.486 kb on + strand, within Pf6N2E2_612at 696.735 kb on - strand, within Pf6N2E2_612at 696.858 kb on + strand, within Pf6N2E2_612at 697.705 kb on - strand, within Pf6N2E2_613at 697.985 kb on + strand, within Pf6N2E2_614at 697.986 kb on - strand, within Pf6N2E2_614at 697.986 kb on - strand, within Pf6N2E2_614at 697.986 kb on - strand, within Pf6N2E2_614at 697.986 kb on - strand, within Pf6N2E2_614at 698.153 kb on + strand, within Pf6N2E2_614at 698.153 kb on + strand, within Pf6N2E2_614at 698.266 kb on + strand, within Pf6N2E2_614at 698.266 kb on + strand, within Pf6N2E2_614at 698.270 kb on + strand, within Pf6N2E2_614at 698.270 kb on + strand, within Pf6N2E2_614at 698.270 kb on + strand, within Pf6N2E2_614at 698.271 kb on - strand, within Pf6N2E2_614at 698.271 kb on - strand, within Pf6N2E2_614

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.5 mM
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694,834 + -0.5
694,835 - -0.3
694,835 - -0.1
695,007 + Pf6N2E2_611 0.18 -0.4
695,245 - Pf6N2E2_611 0.42 -1.2
695,343 + Pf6N2E2_611 0.52 -0.4
695,343 + +0.8
695,343 + Pf6N2E2_611 0.52 +0.0
695,343 + Pf6N2E2_611 0.52 +0.1
695,344 - Pf6N2E2_611 0.52 -0.8
695,344 - Pf6N2E2_611 0.52 -0.3
695,430 + Pf6N2E2_611 0.61 +0.4
695,430 + Pf6N2E2_611 0.61 -0.2
695,431 - Pf6N2E2_611 0.61 +0.4
695,431 - Pf6N2E2_611 0.61 +0.3
695,431 - Pf6N2E2_611 0.61 +0.1
695,436 + Pf6N2E2_611 0.61 +0.2
695,625 + Pf6N2E2_611 0.80 +0.3
695,626 - Pf6N2E2_611 0.80 -0.4
695,817 + -1.0
695,818 - +0.6
695,856 + -0.6
695,856 + -0.2
695,856 + -0.9
695,856 + +0.3
695,856 + +0.0
695,856 + +0.0
695,856 + -0.2
695,856 + +0.6
695,857 - +0.7
695,857 - -1.5
695,857 - -0.1
695,857 - +0.4
695,857 - +1.5
696,308 + Pf6N2E2_612 0.30 -1.0
696,309 - Pf6N2E2_612 0.30 -0.1
696,486 + Pf6N2E2_612 0.41 -1.0
696,735 - Pf6N2E2_612 0.57 -0.1
696,858 + Pf6N2E2_612 0.65 -0.8
697,705 - Pf6N2E2_613 0.51 +0.3
697,985 + Pf6N2E2_614 0.11 -0.5
697,986 - Pf6N2E2_614 0.11 +0.5
697,986 - Pf6N2E2_614 0.11 -1.7
697,986 - Pf6N2E2_614 0.11 +0.3
697,986 - Pf6N2E2_614 0.11 -1.2
698,153 + Pf6N2E2_614 0.25 +1.4
698,153 + Pf6N2E2_614 0.25 +1.2
698,266 + Pf6N2E2_614 0.35 -0.3
698,266 + Pf6N2E2_614 0.35 +0.5
698,270 + Pf6N2E2_614 0.35 -0.4
698,270 + Pf6N2E2_614 0.35 +0.3
698,270 + Pf6N2E2_614 0.35 -0.5
698,271 - Pf6N2E2_614 0.35 -0.6
698,271 - Pf6N2E2_614 0.35 +0.8

Or see this region's nucleotide sequence