Experiment: Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_611 and Pf6N2E2_612 are separated by 12 nucleotides Pf6N2E2_612 and Pf6N2E2_613 are separated by 118 nucleotides Pf6N2E2_613 and Pf6N2E2_614 overlap by 14 nucleotides
Pf6N2E2_611 - 2-dehydro-3-deoxy-L-arabinonate dehydratase (EC 4.2.1.43) (from data) , at 694,827 to 695,819
_611
Pf6N2E2_612 - Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26) (from data) , at 695,832 to 697,412
_612
Pf6N2E2_613 - lipoprotein, putative, at 697,531 to 697,869
_613
Pf6N2E2_614 - 3-carboxymuconate cyclase, at 697,856 to 699,031
_614
Position (kb)
695
696
697
698 Strain fitness (log2 ratio)
-1
0
1 at 694.834 kb on + strand at 694.835 kb on - strand at 694.835 kb on - strand at 695.007 kb on + strand, within Pf6N2E2_611 at 695.245 kb on - strand, within Pf6N2E2_611 at 695.343 kb on + strand, within Pf6N2E2_611 at 695.343 kb on + strand at 695.343 kb on + strand, within Pf6N2E2_611 at 695.343 kb on + strand, within Pf6N2E2_611 at 695.344 kb on - strand, within Pf6N2E2_611 at 695.344 kb on - strand, within Pf6N2E2_611 at 695.430 kb on + strand, within Pf6N2E2_611 at 695.430 kb on + strand, within Pf6N2E2_611 at 695.431 kb on - strand, within Pf6N2E2_611 at 695.431 kb on - strand, within Pf6N2E2_611 at 695.431 kb on - strand, within Pf6N2E2_611 at 695.436 kb on + strand, within Pf6N2E2_611 at 695.625 kb on + strand, within Pf6N2E2_611 at 695.626 kb on - strand, within Pf6N2E2_611 at 695.817 kb on + strand at 695.818 kb on - strand at 695.856 kb on + strand at 695.856 kb on + strand at 695.856 kb on + strand at 695.856 kb on + strand at 695.856 kb on + strand at 695.856 kb on + strand at 695.856 kb on + strand at 695.856 kb on + strand at 695.857 kb on - strand at 695.857 kb on - strand at 695.857 kb on - strand at 695.857 kb on - strand at 695.857 kb on - strand at 696.308 kb on + strand, within Pf6N2E2_612 at 696.309 kb on - strand, within Pf6N2E2_612 at 696.486 kb on + strand, within Pf6N2E2_612 at 696.735 kb on - strand, within Pf6N2E2_612 at 696.858 kb on + strand, within Pf6N2E2_612 at 697.705 kb on - strand, within Pf6N2E2_613 at 697.985 kb on + strand, within Pf6N2E2_614 at 697.986 kb on - strand, within Pf6N2E2_614 at 697.986 kb on - strand, within Pf6N2E2_614 at 697.986 kb on - strand, within Pf6N2E2_614 at 697.986 kb on - strand, within Pf6N2E2_614 at 698.153 kb on + strand, within Pf6N2E2_614 at 698.153 kb on + strand, within Pf6N2E2_614 at 698.266 kb on + strand, within Pf6N2E2_614 at 698.266 kb on + strand, within Pf6N2E2_614 at 698.270 kb on + strand, within Pf6N2E2_614 at 698.270 kb on + strand, within Pf6N2E2_614 at 698.270 kb on + strand, within Pf6N2E2_614 at 698.271 kb on - strand, within Pf6N2E2_614 at 698.271 kb on - strand, within Pf6N2E2_614
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.5 mM remove 694,834 + -0.5 694,835 - -0.3 694,835 - -0.1 695,007 + Pf6N2E2_611 0.18 -0.4 695,245 - Pf6N2E2_611 0.42 -1.2 695,343 + Pf6N2E2_611 0.52 -0.4 695,343 + +0.8 695,343 + Pf6N2E2_611 0.52 +0.0 695,343 + Pf6N2E2_611 0.52 +0.1 695,344 - Pf6N2E2_611 0.52 -0.8 695,344 - Pf6N2E2_611 0.52 -0.3 695,430 + Pf6N2E2_611 0.61 +0.4 695,430 + Pf6N2E2_611 0.61 -0.2 695,431 - Pf6N2E2_611 0.61 +0.4 695,431 - Pf6N2E2_611 0.61 +0.3 695,431 - Pf6N2E2_611 0.61 +0.1 695,436 + Pf6N2E2_611 0.61 +0.2 695,625 + Pf6N2E2_611 0.80 +0.3 695,626 - Pf6N2E2_611 0.80 -0.4 695,817 + -1.0 695,818 - +0.6 695,856 + -0.6 695,856 + -0.2 695,856 + -0.9 695,856 + +0.3 695,856 + +0.0 695,856 + +0.0 695,856 + -0.2 695,856 + +0.6 695,857 - +0.7 695,857 - -1.5 695,857 - -0.1 695,857 - +0.4 695,857 - +1.5 696,308 + Pf6N2E2_612 0.30 -1.0 696,309 - Pf6N2E2_612 0.30 -0.1 696,486 + Pf6N2E2_612 0.41 -1.0 696,735 - Pf6N2E2_612 0.57 -0.1 696,858 + Pf6N2E2_612 0.65 -0.8 697,705 - Pf6N2E2_613 0.51 +0.3 697,985 + Pf6N2E2_614 0.11 -0.5 697,986 - Pf6N2E2_614 0.11 +0.5 697,986 - Pf6N2E2_614 0.11 -1.7 697,986 - Pf6N2E2_614 0.11 +0.3 697,986 - Pf6N2E2_614 0.11 -1.2 698,153 + Pf6N2E2_614 0.25 +1.4 698,153 + Pf6N2E2_614 0.25 +1.2 698,266 + Pf6N2E2_614 0.35 -0.3 698,266 + Pf6N2E2_614 0.35 +0.5 698,270 + Pf6N2E2_614 0.35 -0.4 698,270 + Pf6N2E2_614 0.35 +0.3 698,270 + Pf6N2E2_614 0.35 -0.5 698,271 - Pf6N2E2_614 0.35 -0.6 698,271 - Pf6N2E2_614 0.35 +0.8
Or see this region's nucleotide sequence