Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5742

Experiment: Nickel (II) chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5740 and Pf6N2E2_5741 overlap by 8 nucleotidesPf6N2E2_5741 and Pf6N2E2_5742 overlap by 4 nucleotidesPf6N2E2_5742 and Pf6N2E2_5743 are separated by 35 nucleotidesPf6N2E2_5743 and Pf6N2E2_5744 overlap by 4 nucleotides Pf6N2E2_5740 - Adenosylcobinamide-phosphate synthase (EC 6.3.1.10), at 6,556,816 to 6,557,739 _5740 Pf6N2E2_5741 - L-threonine 3-O-phosphate decarboxylase (EC 4.1.1.81), at 6,557,732 to 6,558,724 _5741 Pf6N2E2_5742 - Cobyric acid synthase (EC 6.3.5.10), at 6,558,721 to 6,560,172 _5742 Pf6N2E2_5743 - Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62), at 6,560,208 to 6,560,729 _5743 Pf6N2E2_5744 - Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (EC 2.4.2.21), at 6,560,726 to 6,561,781 _5744 Position (kb) 6558 6559 6560 6561Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 6557.731 kb on + strandat 6557.731 kb on + strandat 6557.731 kb on + strandat 6557.913 kb on - strand, within Pf6N2E2_5741at 6557.915 kb on + strand, within Pf6N2E2_5741at 6557.915 kb on + strand, within Pf6N2E2_5741at 6557.915 kb on + strand, within Pf6N2E2_5741at 6557.915 kb on + strand, within Pf6N2E2_5741at 6557.931 kb on + strand, within Pf6N2E2_5741at 6557.931 kb on + strand, within Pf6N2E2_5741at 6557.931 kb on + strand, within Pf6N2E2_5741at 6557.931 kb on + strand, within Pf6N2E2_5741at 6557.932 kb on - strand, within Pf6N2E2_5741at 6557.932 kb on - strand, within Pf6N2E2_5741at 6557.932 kb on - strand, within Pf6N2E2_5741at 6558.053 kb on + strand, within Pf6N2E2_5741at 6558.053 kb on + strand, within Pf6N2E2_5741at 6558.342 kb on + strand, within Pf6N2E2_5741at 6558.343 kb on - strand, within Pf6N2E2_5741at 6558.343 kb on - strand, within Pf6N2E2_5741at 6558.343 kb on - strand, within Pf6N2E2_5741at 6558.626 kb on - strandat 6558.697 kb on - strandat 6558.697 kb on - strandat 6558.697 kb on - strandat 6558.704 kb on + strandat 6558.704 kb on + strandat 6558.705 kb on - strandat 6558.785 kb on + strandat 6559.027 kb on + strand, within Pf6N2E2_5742at 6559.028 kb on - strand, within Pf6N2E2_5742at 6559.028 kb on - strand, within Pf6N2E2_5742at 6559.036 kb on + strand, within Pf6N2E2_5742at 6559.230 kb on - strand, within Pf6N2E2_5742at 6559.393 kb on + strand, within Pf6N2E2_5742at 6559.633 kb on + strand, within Pf6N2E2_5742at 6559.633 kb on + strand, within Pf6N2E2_5742at 6559.633 kb on + strand, within Pf6N2E2_5742at 6559.933 kb on + strand, within Pf6N2E2_5742at 6559.934 kb on - strand, within Pf6N2E2_5742at 6559.993 kb on + strand, within Pf6N2E2_5742at 6559.993 kb on + strand, within Pf6N2E2_5742at 6559.993 kb on + strand, within Pf6N2E2_5742at 6559.993 kb on + strand, within Pf6N2E2_5742at 6559.993 kb on + strand, within Pf6N2E2_5742at 6559.994 kb on - strand, within Pf6N2E2_5742at 6559.994 kb on - strand, within Pf6N2E2_5742at 6559.994 kb on - strand, within Pf6N2E2_5742at 6559.994 kb on - strand, within Pf6N2E2_5742at 6559.994 kb on - strand, within Pf6N2E2_5742at 6560.077 kb on + strandat 6560.078 kb on - strandat 6560.295 kb on + strand, within Pf6N2E2_5743at 6560.295 kb on + strand, within Pf6N2E2_5743at 6560.295 kb on + strand, within Pf6N2E2_5743at 6560.295 kb on + strand, within Pf6N2E2_5743at 6560.295 kb on + strand, within Pf6N2E2_5743at 6560.296 kb on - strand, within Pf6N2E2_5743at 6560.296 kb on - strand, within Pf6N2E2_5743at 6560.296 kb on - strand, within Pf6N2E2_5743at 6560.296 kb on - strand, within Pf6N2E2_5743at 6560.296 kb on - strand, within Pf6N2E2_5743at 6560.296 kb on - strand, within Pf6N2E2_5743at 6560.296 kb on - strand, within Pf6N2E2_5743at 6560.296 kb on - strand, within Pf6N2E2_5743at 6560.296 kb on - strand, within Pf6N2E2_5743at 6560.622 kb on + strand, within Pf6N2E2_5743at 6560.717 kb on + strandat 6560.725 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.5 mM
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6,557,731 + -0.7
6,557,731 + -0.8
6,557,731 + -0.4
6,557,913 - Pf6N2E2_5741 0.18 +0.0
6,557,915 + Pf6N2E2_5741 0.18 -0.5
6,557,915 + Pf6N2E2_5741 0.18 -1.2
6,557,915 + Pf6N2E2_5741 0.18 +0.8
6,557,915 + Pf6N2E2_5741 0.18 -0.4
6,557,931 + Pf6N2E2_5741 0.20 -1.0
6,557,931 + Pf6N2E2_5741 0.20 -2.0
6,557,931 + Pf6N2E2_5741 0.20 +0.0
6,557,931 + Pf6N2E2_5741 0.20 -0.2
6,557,932 - Pf6N2E2_5741 0.20 -2.0
6,557,932 - Pf6N2E2_5741 0.20 +1.2
6,557,932 - Pf6N2E2_5741 0.20 -1.0
6,558,053 + Pf6N2E2_5741 0.32 -1.0
6,558,053 + Pf6N2E2_5741 0.32 -0.1
6,558,342 + Pf6N2E2_5741 0.61 -0.5
6,558,343 - Pf6N2E2_5741 0.62 -0.1
6,558,343 - Pf6N2E2_5741 0.62 +1.1
6,558,343 - Pf6N2E2_5741 0.62 -0.5
6,558,626 - -1.2
6,558,697 - -1.3
6,558,697 - -0.0
6,558,697 - -0.6
6,558,704 + +0.5
6,558,704 + +1.3
6,558,705 - +1.6
6,558,785 + -0.4
6,559,027 + Pf6N2E2_5742 0.21 -0.2
6,559,028 - Pf6N2E2_5742 0.21 -1.0
6,559,028 - Pf6N2E2_5742 0.21 -1.2
6,559,036 + Pf6N2E2_5742 0.22 -1.6
6,559,230 - Pf6N2E2_5742 0.35 -1.1
6,559,393 + Pf6N2E2_5742 0.46 -1.3
6,559,633 + Pf6N2E2_5742 0.63 -0.4
6,559,633 + Pf6N2E2_5742 0.63 -1.6
6,559,633 + Pf6N2E2_5742 0.63 -1.0
6,559,933 + Pf6N2E2_5742 0.83 -2.1
6,559,934 - Pf6N2E2_5742 0.84 -2.9
6,559,993 + Pf6N2E2_5742 0.88 -0.6
6,559,993 + Pf6N2E2_5742 0.88 -0.3
6,559,993 + Pf6N2E2_5742 0.88 -1.3
6,559,993 + Pf6N2E2_5742 0.88 -1.1
6,559,993 + Pf6N2E2_5742 0.88 -0.1
6,559,994 - Pf6N2E2_5742 0.88 -0.2
6,559,994 - Pf6N2E2_5742 0.88 -1.7
6,559,994 - Pf6N2E2_5742 0.88 -2.0
6,559,994 - Pf6N2E2_5742 0.88 +0.6
6,559,994 - Pf6N2E2_5742 0.88 -2.4
6,560,077 + +0.5
6,560,078 - -0.6
6,560,295 + Pf6N2E2_5743 0.17 -0.7
6,560,295 + Pf6N2E2_5743 0.17 +0.1
6,560,295 + Pf6N2E2_5743 0.17 -0.3
6,560,295 + Pf6N2E2_5743 0.17 -0.2
6,560,295 + Pf6N2E2_5743 0.17 -2.5
6,560,296 - Pf6N2E2_5743 0.17 -1.1
6,560,296 - Pf6N2E2_5743 0.17 -1.5
6,560,296 - Pf6N2E2_5743 0.17 +0.9
6,560,296 - Pf6N2E2_5743 0.17 +1.9
6,560,296 - Pf6N2E2_5743 0.17 -0.7
6,560,296 - Pf6N2E2_5743 0.17 -0.0
6,560,296 - Pf6N2E2_5743 0.17 -0.8
6,560,296 - Pf6N2E2_5743 0.17 -0.3
6,560,296 - Pf6N2E2_5743 0.17 +0.1
6,560,622 + Pf6N2E2_5743 0.79 -0.2
6,560,717 + -0.2
6,560,725 + +0.1

Or see this region's nucleotide sequence