Experiment: Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3210 and Pf6N2E2_3211 overlap by 17 nucleotides Pf6N2E2_3211 and Pf6N2E2_3212 are separated by 1 nucleotides
Pf6N2E2_3210 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 3,707,891 to 3,708,976
_3210
Pf6N2E2_3211 - Outer membrane receptor proteins, mostly Fe transport, at 3,708,960 to 3,711,311
_3211
Pf6N2E2_3212 - Signal transduction histidine kinase, at 3,711,313 to 3,713,712
_3212
Position (kb)
3708
3709
3710
3711
3712 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3707.990 kb on + strand at 3707.991 kb on - strand at 3708.255 kb on - strand, within Pf6N2E2_3210 at 3708.255 kb on - strand, within Pf6N2E2_3210 at 3708.380 kb on - strand, within Pf6N2E2_3210 at 3708.392 kb on + strand, within Pf6N2E2_3210 at 3708.392 kb on + strand, within Pf6N2E2_3210 at 3708.392 kb on + strand, within Pf6N2E2_3210 at 3708.393 kb on - strand, within Pf6N2E2_3210 at 3708.393 kb on - strand, within Pf6N2E2_3210 at 3708.393 kb on - strand, within Pf6N2E2_3210 at 3708.393 kb on - strand, within Pf6N2E2_3210 at 3708.442 kb on - strand, within Pf6N2E2_3210 at 3708.442 kb on - strand, within Pf6N2E2_3210 at 3708.602 kb on + strand, within Pf6N2E2_3210 at 3708.602 kb on + strand, within Pf6N2E2_3210 at 3708.603 kb on - strand, within Pf6N2E2_3210 at 3708.899 kb on + strand at 3708.899 kb on + strand at 3708.900 kb on - strand at 3708.900 kb on - strand at 3708.900 kb on - strand at 3708.952 kb on + strand at 3708.952 kb on + strand at 3708.953 kb on - strand at 3709.018 kb on + strand at 3709.019 kb on - strand at 3709.042 kb on + strand at 3709.042 kb on + strand at 3709.042 kb on + strand at 3709.057 kb on + strand at 3709.057 kb on + strand at 3709.058 kb on - strand at 3709.148 kb on - strand at 3709.159 kb on + strand at 3709.159 kb on + strand at 3709.160 kb on - strand at 3709.160 kb on - strand at 3709.210 kb on + strand, within Pf6N2E2_3211 at 3709.211 kb on - strand, within Pf6N2E2_3211 at 3709.297 kb on + strand, within Pf6N2E2_3211 at 3709.297 kb on + strand, within Pf6N2E2_3211 at 3709.297 kb on + strand, within Pf6N2E2_3211 at 3709.297 kb on + strand, within Pf6N2E2_3211 at 3709.297 kb on + strand, within Pf6N2E2_3211 at 3709.525 kb on + strand, within Pf6N2E2_3211 at 3709.525 kb on + strand, within Pf6N2E2_3211 at 3709.525 kb on + strand, within Pf6N2E2_3211 at 3709.525 kb on + strand, within Pf6N2E2_3211 at 3709.529 kb on + strand, within Pf6N2E2_3211 at 3709.529 kb on + strand, within Pf6N2E2_3211 at 3709.529 kb on + strand, within Pf6N2E2_3211 at 3709.530 kb on - strand, within Pf6N2E2_3211 at 3709.540 kb on + strand, within Pf6N2E2_3211 at 3709.540 kb on + strand, within Pf6N2E2_3211 at 3709.541 kb on - strand, within Pf6N2E2_3211 at 3709.561 kb on + strand, within Pf6N2E2_3211 at 3709.700 kb on - strand, within Pf6N2E2_3211 at 3709.720 kb on + strand, within Pf6N2E2_3211 at 3709.720 kb on + strand, within Pf6N2E2_3211 at 3709.721 kb on - strand, within Pf6N2E2_3211 at 3709.747 kb on + strand, within Pf6N2E2_3211 at 3710.023 kb on + strand, within Pf6N2E2_3211 at 3710.089 kb on + strand, within Pf6N2E2_3211 at 3710.189 kb on - strand, within Pf6N2E2_3211 at 3710.191 kb on + strand, within Pf6N2E2_3211 at 3710.192 kb on - strand, within Pf6N2E2_3211 at 3710.192 kb on - strand, within Pf6N2E2_3211 at 3710.206 kb on + strand, within Pf6N2E2_3211 at 3710.461 kb on + strand, within Pf6N2E2_3211 at 3710.462 kb on - strand, within Pf6N2E2_3211 at 3710.462 kb on - strand, within Pf6N2E2_3211 at 3710.569 kb on + strand, within Pf6N2E2_3211 at 3710.971 kb on + strand, within Pf6N2E2_3211 at 3710.971 kb on + strand, within Pf6N2E2_3211 at 3710.971 kb on + strand, within Pf6N2E2_3211 at 3710.971 kb on + strand, within Pf6N2E2_3211 at 3710.971 kb on + strand, within Pf6N2E2_3211 at 3710.971 kb on + strand, within Pf6N2E2_3211 at 3710.971 kb on + strand, within Pf6N2E2_3211 at 3710.971 kb on + strand, within Pf6N2E2_3211 at 3710.972 kb on - strand, within Pf6N2E2_3211 at 3710.972 kb on - strand, within Pf6N2E2_3211 at 3711.314 kb on + strand at 3711.314 kb on + strand at 3711.314 kb on + strand at 3711.636 kb on - strand, within Pf6N2E2_3212 at 3711.654 kb on - strand, within Pf6N2E2_3212 at 3711.702 kb on + strand, within Pf6N2E2_3212 at 3711.891 kb on - strand, within Pf6N2E2_3212 at 3711.891 kb on - strand, within Pf6N2E2_3212 at 3712.198 kb on + strand, within Pf6N2E2_3212 at 3712.198 kb on + strand, within Pf6N2E2_3212 at 3712.199 kb on - strand, within Pf6N2E2_3212 at 3712.218 kb on - strand, within Pf6N2E2_3212 at 3712.219 kb on + strand, within Pf6N2E2_3212 at 3712.219 kb on + strand, within Pf6N2E2_3212 at 3712.219 kb on + strand, within Pf6N2E2_3212 at 3712.219 kb on + strand, within Pf6N2E2_3212 at 3712.219 kb on + strand, within Pf6N2E2_3212 at 3712.219 kb on + strand, within Pf6N2E2_3212 at 3712.220 kb on - strand, within Pf6N2E2_3212 at 3712.220 kb on - strand, within Pf6N2E2_3212 at 3712.220 kb on - strand, within Pf6N2E2_3212 at 3712.220 kb on - strand, within Pf6N2E2_3212 at 3712.220 kb on - strand, within Pf6N2E2_3212 at 3712.223 kb on + strand, within Pf6N2E2_3212 at 3712.224 kb on - strand, within Pf6N2E2_3212
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.5 mM remove 3,707,990 + +0.2 3,707,991 - +0.6 3,708,255 - Pf6N2E2_3210 0.34 +0.6 3,708,255 - Pf6N2E2_3210 0.34 -1.0 3,708,380 - Pf6N2E2_3210 0.45 +0.6 3,708,392 + Pf6N2E2_3210 0.46 -0.4 3,708,392 + Pf6N2E2_3210 0.46 -0.1 3,708,392 + Pf6N2E2_3210 0.46 -0.3 3,708,393 - Pf6N2E2_3210 0.46 +0.4 3,708,393 - Pf6N2E2_3210 0.46 +0.2 3,708,393 - Pf6N2E2_3210 0.46 -0.3 3,708,393 - Pf6N2E2_3210 0.46 +0.4 3,708,442 - Pf6N2E2_3210 0.51 +0.3 3,708,442 - Pf6N2E2_3210 0.51 -0.8 3,708,602 + Pf6N2E2_3210 0.65 -0.2 3,708,602 + Pf6N2E2_3210 0.65 -0.9 3,708,603 - Pf6N2E2_3210 0.66 +0.3 3,708,899 + -0.1 3,708,899 + +0.9 3,708,900 - -0.7 3,708,900 - +0.8 3,708,900 - +0.3 3,708,952 + +1.0 3,708,952 + -0.9 3,708,953 - +1.1 3,709,018 + -0.1 3,709,019 - +1.3 3,709,042 + +0.4 3,709,042 + +0.9 3,709,042 + +0.6 3,709,057 + +0.8 3,709,057 + -0.3 3,709,058 - +0.2 3,709,148 - +0.1 3,709,159 + -0.9 3,709,159 + -0.5 3,709,160 - +0.3 3,709,160 - +0.2 3,709,210 + Pf6N2E2_3211 0.11 +0.4 3,709,211 - Pf6N2E2_3211 0.11 +0.9 3,709,297 + Pf6N2E2_3211 0.14 -1.0 3,709,297 + Pf6N2E2_3211 0.14 -0.4 3,709,297 + Pf6N2E2_3211 0.14 -0.9 3,709,297 + Pf6N2E2_3211 0.14 -0.7 3,709,297 + Pf6N2E2_3211 0.14 -0.2 3,709,525 + Pf6N2E2_3211 0.24 -0.4 3,709,525 + Pf6N2E2_3211 0.24 -0.3 3,709,525 + Pf6N2E2_3211 0.24 +0.4 3,709,525 + Pf6N2E2_3211 0.24 +0.6 3,709,529 + Pf6N2E2_3211 0.24 +0.5 3,709,529 + Pf6N2E2_3211 0.24 -0.0 3,709,529 + Pf6N2E2_3211 0.24 -0.3 3,709,530 - Pf6N2E2_3211 0.24 -0.2 3,709,540 + Pf6N2E2_3211 0.25 -0.4 3,709,540 + Pf6N2E2_3211 0.25 -1.4 3,709,541 - Pf6N2E2_3211 0.25 -0.7 3,709,561 + Pf6N2E2_3211 0.26 +0.5 3,709,700 - Pf6N2E2_3211 0.31 +0.6 3,709,720 + Pf6N2E2_3211 0.32 +0.3 3,709,720 + Pf6N2E2_3211 0.32 -0.0 3,709,721 - Pf6N2E2_3211 0.32 -0.7 3,709,747 + Pf6N2E2_3211 0.33 -0.2 3,710,023 + Pf6N2E2_3211 0.45 -0.4 3,710,089 + Pf6N2E2_3211 0.48 -0.7 3,710,189 - Pf6N2E2_3211 0.52 +0.4 3,710,191 + Pf6N2E2_3211 0.52 -0.8 3,710,192 - Pf6N2E2_3211 0.52 -0.2 3,710,192 - Pf6N2E2_3211 0.52 -0.5 3,710,206 + Pf6N2E2_3211 0.53 +1.4 3,710,461 + Pf6N2E2_3211 0.64 +0.1 3,710,462 - Pf6N2E2_3211 0.64 +0.3 3,710,462 - Pf6N2E2_3211 0.64 -0.8 3,710,569 + Pf6N2E2_3211 0.68 -0.7 3,710,971 + Pf6N2E2_3211 0.86 +0.2 3,710,971 + Pf6N2E2_3211 0.86 -0.4 3,710,971 + Pf6N2E2_3211 0.86 -0.4 3,710,971 + Pf6N2E2_3211 0.86 +0.1 3,710,971 + Pf6N2E2_3211 0.86 +0.3 3,710,971 + Pf6N2E2_3211 0.86 -0.5 3,710,971 + Pf6N2E2_3211 0.86 -3.3 3,710,971 + Pf6N2E2_3211 0.86 -1.1 3,710,972 - Pf6N2E2_3211 0.86 -0.8 3,710,972 - Pf6N2E2_3211 0.86 -1.1 3,711,314 + +0.3 3,711,314 + -1.6 3,711,314 + +0.4 3,711,636 - Pf6N2E2_3212 0.13 +0.2 3,711,654 - Pf6N2E2_3212 0.14 +0.2 3,711,702 + Pf6N2E2_3212 0.16 -1.1 3,711,891 - Pf6N2E2_3212 0.24 -0.1 3,711,891 - Pf6N2E2_3212 0.24 -0.5 3,712,198 + Pf6N2E2_3212 0.37 +0.1 3,712,198 + Pf6N2E2_3212 0.37 +0.2 3,712,199 - Pf6N2E2_3212 0.37 -0.6 3,712,218 - Pf6N2E2_3212 0.38 +0.0 3,712,219 + Pf6N2E2_3212 0.38 +1.3 3,712,219 + Pf6N2E2_3212 0.38 -0.3 3,712,219 + Pf6N2E2_3212 0.38 +0.8 3,712,219 + Pf6N2E2_3212 0.38 -0.8 3,712,219 + Pf6N2E2_3212 0.38 -0.8 3,712,219 + Pf6N2E2_3212 0.38 +0.6 3,712,220 - Pf6N2E2_3212 0.38 -0.2 3,712,220 - Pf6N2E2_3212 0.38 +0.8 3,712,220 - Pf6N2E2_3212 0.38 +0.1 3,712,220 - Pf6N2E2_3212 0.38 +0.5 3,712,220 - Pf6N2E2_3212 0.38 -0.4 3,712,223 + Pf6N2E2_3212 0.38 +0.5 3,712,224 - Pf6N2E2_3212 0.38 -0.4
Or see this region's nucleotide sequence