Experiment: Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3103 and Pf6N2E2_3104 are separated by 173 nucleotides Pf6N2E2_3104 and Pf6N2E2_3105 are separated by 88 nucleotides Pf6N2E2_3105 and Pf6N2E2_3106 are separated by 34 nucleotides Pf6N2E2_3106 and Pf6N2E2_3107 are separated by 990 nucleotides
Pf6N2E2_3103 - Phosphoenolpyruvate carboxylase (EC 4.1.1.31), at 3,588,937 to 3,591,573
_3103
Pf6N2E2_3104 - FIG00958830: hypothetical protein, at 3,591,747 to 3,592,055
_3104
Pf6N2E2_3105 - hypothetical protein, at 3,592,144 to 3,592,464
_3105
Pf6N2E2_3106 - hypothetical protein, at 3,592,499 to 3,594,271
_3106
Pf6N2E2_3107 - unknown, without homologous in databases, at 3,595,262 to 3,596,917
_3107
Position (kb)
3592
3593
3594
3595 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3591.514 kb on + strand at 3591.514 kb on + strand at 3591.607 kb on + strand at 3591.607 kb on + strand at 3591.607 kb on + strand at 3591.608 kb on - strand at 3591.654 kb on + strand at 3591.655 kb on - strand at 3591.655 kb on - strand at 3591.655 kb on - strand at 3591.674 kb on + strand at 3591.674 kb on + strand at 3591.675 kb on - strand at 3591.686 kb on + strand at 3591.687 kb on - strand at 3591.813 kb on + strand, within Pf6N2E2_3104 at 3591.870 kb on + strand, within Pf6N2E2_3104 at 3591.870 kb on + strand, within Pf6N2E2_3104 at 3591.870 kb on + strand, within Pf6N2E2_3104 at 3591.870 kb on + strand, within Pf6N2E2_3104 at 3591.871 kb on - strand, within Pf6N2E2_3104 at 3591.871 kb on - strand, within Pf6N2E2_3104 at 3591.871 kb on - strand, within Pf6N2E2_3104 at 3591.948 kb on + strand, within Pf6N2E2_3104 at 3591.948 kb on + strand, within Pf6N2E2_3104 at 3592.000 kb on - strand, within Pf6N2E2_3104 at 3592.022 kb on - strand, within Pf6N2E2_3104 at 3592.054 kb on - strand at 3592.107 kb on - strand at 3592.112 kb on + strand at 3592.112 kb on + strand at 3592.112 kb on + strand at 3592.112 kb on + strand at 3592.123 kb on + strand at 3592.124 kb on - strand at 3592.124 kb on - strand at 3592.124 kb on - strand at 3592.241 kb on + strand, within Pf6N2E2_3105 at 3592.270 kb on + strand, within Pf6N2E2_3105 at 3592.277 kb on + strand, within Pf6N2E2_3105 at 3592.277 kb on + strand, within Pf6N2E2_3105 at 3592.278 kb on - strand, within Pf6N2E2_3105 at 3592.317 kb on + strand, within Pf6N2E2_3105 at 3592.318 kb on - strand, within Pf6N2E2_3105 at 3592.319 kb on + strand, within Pf6N2E2_3105 at 3592.320 kb on - strand, within Pf6N2E2_3105 at 3592.320 kb on - strand, within Pf6N2E2_3105 at 3592.420 kb on + strand, within Pf6N2E2_3105 at 3592.420 kb on + strand, within Pf6N2E2_3105 at 3592.462 kb on + strand at 3592.484 kb on + strand at 3592.500 kb on + strand at 3592.527 kb on - strand at 3592.549 kb on - strand at 3592.574 kb on - strand at 3592.685 kb on - strand, within Pf6N2E2_3106 at 3592.834 kb on - strand, within Pf6N2E2_3106 at 3593.065 kb on + strand, within Pf6N2E2_3106 at 3593.396 kb on - strand, within Pf6N2E2_3106 at 3593.448 kb on + strand, within Pf6N2E2_3106 at 3593.480 kb on - strand, within Pf6N2E2_3106 at 3593.799 kb on - strand, within Pf6N2E2_3106 at 3593.849 kb on - strand, within Pf6N2E2_3106 at 3594.487 kb on + strand at 3594.638 kb on + strand at 3594.639 kb on - strand at 3594.661 kb on + strand at 3594.662 kb on - strand at 3594.662 kb on - strand at 3594.694 kb on - strand at 3594.748 kb on + strand at 3594.749 kb on - strand at 3594.763 kb on - strand at 3594.763 kb on - strand at 3594.903 kb on + strand at 3595.001 kb on + strand at 3595.009 kb on - strand at 3595.166 kb on - strand at 3595.166 kb on - strand at 3595.271 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.5 mM remove 3,591,514 + -0.9 3,591,514 + -0.6 3,591,607 + +0.7 3,591,607 + +0.3 3,591,607 + +0.3 3,591,608 - -1.4 3,591,654 + +0.5 3,591,655 - -0.1 3,591,655 - -0.3 3,591,655 - -1.0 3,591,674 + +0.8 3,591,674 + +0.5 3,591,675 - -0.2 3,591,686 + +0.4 3,591,687 - -1.0 3,591,813 + Pf6N2E2_3104 0.21 -0.1 3,591,870 + Pf6N2E2_3104 0.40 -2.0 3,591,870 + Pf6N2E2_3104 0.40 -0.7 3,591,870 + Pf6N2E2_3104 0.40 +0.2 3,591,870 + Pf6N2E2_3104 0.40 +0.1 3,591,871 - Pf6N2E2_3104 0.40 -0.1 3,591,871 - Pf6N2E2_3104 0.40 -1.0 3,591,871 - Pf6N2E2_3104 0.40 -0.6 3,591,948 + Pf6N2E2_3104 0.65 +0.0 3,591,948 + Pf6N2E2_3104 0.65 -0.3 3,592,000 - Pf6N2E2_3104 0.82 -0.3 3,592,022 - Pf6N2E2_3104 0.89 +0.8 3,592,054 - -1.6 3,592,107 - -0.2 3,592,112 + -0.5 3,592,112 + -0.2 3,592,112 + -0.7 3,592,112 + +1.3 3,592,123 + +0.3 3,592,124 - -0.3 3,592,124 - -0.1 3,592,124 - -1.0 3,592,241 + Pf6N2E2_3105 0.30 +1.9 3,592,270 + Pf6N2E2_3105 0.39 -0.8 3,592,277 + Pf6N2E2_3105 0.41 -0.2 3,592,277 + Pf6N2E2_3105 0.41 +0.3 3,592,278 - Pf6N2E2_3105 0.42 +0.6 3,592,317 + Pf6N2E2_3105 0.54 +0.3 3,592,318 - Pf6N2E2_3105 0.54 +1.5 3,592,319 + Pf6N2E2_3105 0.55 -0.1 3,592,320 - Pf6N2E2_3105 0.55 -0.0 3,592,320 - Pf6N2E2_3105 0.55 +2.1 3,592,420 + Pf6N2E2_3105 0.86 +0.0 3,592,420 + Pf6N2E2_3105 0.86 +0.5 3,592,462 + +1.3 3,592,484 + +1.3 3,592,500 + +0.3 3,592,527 - +0.2 3,592,549 - -0.0 3,592,574 - -1.7 3,592,685 - Pf6N2E2_3106 0.10 -0.3 3,592,834 - Pf6N2E2_3106 0.19 -0.0 3,593,065 + Pf6N2E2_3106 0.32 -0.6 3,593,396 - Pf6N2E2_3106 0.51 -0.4 3,593,448 + Pf6N2E2_3106 0.54 -1.1 3,593,480 - Pf6N2E2_3106 0.55 +0.8 3,593,799 - Pf6N2E2_3106 0.73 -1.4 3,593,849 - Pf6N2E2_3106 0.76 -0.3 3,594,487 + +0.3 3,594,638 + -0.8 3,594,639 - +0.8 3,594,661 + -0.7 3,594,662 - +0.7 3,594,662 - -1.0 3,594,694 - +0.6 3,594,748 + +0.1 3,594,749 - -0.7 3,594,763 - +0.3 3,594,763 - -0.4 3,594,903 + -0.3 3,595,001 + +0.2 3,595,009 - +0.3 3,595,166 - -0.5 3,595,166 - -0.1 3,595,271 + +0.8
Or see this region's nucleotide sequence