Experiment: Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3025 and Pf6N2E2_3026 overlap by 4 nucleotides Pf6N2E2_3026 and Pf6N2E2_3027 are separated by 11 nucleotides Pf6N2E2_3027 and Pf6N2E2_3028 are separated by 3 nucleotides Pf6N2E2_3028 and Pf6N2E2_3029 are separated by 36 nucleotides Pf6N2E2_3029 and Pf6N2E2_3030 are separated by 119 nucleotides
Pf6N2E2_3025 - Baseplate assembly protein J, at 3,511,244 to 3,512,239
_3025
Pf6N2E2_3026 - Baseplate assembly protein W, at 3,512,236 to 3,512,568
_3026
Pf6N2E2_3027 - Baseplate assembly protein V, at 3,512,580 to 3,513,191
_3027
Pf6N2E2_3028 - FIG00959937: hypothetical protein, at 3,513,195 to 3,513,707
_3028
Pf6N2E2_3029 - Holin, at 3,513,744 to 3,514,094
_3029
Pf6N2E2_3030 - hypothetical protein, at 3,514,214 to 3,514,642
_3030
Position (kb)
3513
3514 Strain fitness (log2 ratio)
-1
0
1
2 at 3512.366 kb on + strand, within Pf6N2E2_3026 at 3512.367 kb on - strand, within Pf6N2E2_3026 at 3512.453 kb on + strand, within Pf6N2E2_3026 at 3512.453 kb on + strand, within Pf6N2E2_3026 at 3512.454 kb on - strand, within Pf6N2E2_3026 at 3512.454 kb on - strand, within Pf6N2E2_3026 at 3512.454 kb on - strand, within Pf6N2E2_3026 at 3512.454 kb on - strand, within Pf6N2E2_3026 at 3512.454 kb on - strand, within Pf6N2E2_3026 at 3512.512 kb on + strand, within Pf6N2E2_3026 at 3512.512 kb on + strand, within Pf6N2E2_3026 at 3512.548 kb on - strand at 3512.582 kb on - strand at 3512.721 kb on - strand, within Pf6N2E2_3027 at 3513.214 kb on + strand at 3513.214 kb on + strand at 3513.214 kb on + strand at 3513.240 kb on + strand at 3513.252 kb on - strand, within Pf6N2E2_3028 at 3513.305 kb on - strand, within Pf6N2E2_3028 at 3513.340 kb on + strand, within Pf6N2E2_3028 at 3513.341 kb on - strand, within Pf6N2E2_3028 at 3513.341 kb on - strand, within Pf6N2E2_3028 at 3513.341 kb on - strand, within Pf6N2E2_3028 at 3513.341 kb on - strand, within Pf6N2E2_3028 at 3513.640 kb on + strand, within Pf6N2E2_3028 at 3513.641 kb on - strand, within Pf6N2E2_3028 at 3513.724 kb on + strand at 3513.724 kb on + strand at 3513.725 kb on - strand at 3513.901 kb on + strand, within Pf6N2E2_3029 at 3513.901 kb on + strand, within Pf6N2E2_3029 at 3513.901 kb on + strand, within Pf6N2E2_3029 at 3513.901 kb on + strand, within Pf6N2E2_3029 at 3513.901 kb on + strand, within Pf6N2E2_3029 at 3513.901 kb on + strand, within Pf6N2E2_3029 at 3513.901 kb on + strand, within Pf6N2E2_3029 at 3513.902 kb on - strand, within Pf6N2E2_3029 at 3513.902 kb on - strand, within Pf6N2E2_3029 at 3514.116 kb on + strand at 3514.117 kb on - strand at 3514.136 kb on - strand at 3514.163 kb on - strand at 3514.192 kb on - strand at 3514.192 kb on - strand at 3514.215 kb on + strand at 3514.215 kb on + strand at 3514.216 kb on - strand at 3514.216 kb on - strand at 3514.216 kb on - strand at 3514.216 kb on - strand at 3514.245 kb on + strand at 3514.245 kb on + strand at 3514.245 kb on + strand at 3514.245 kb on + strand at 3514.245 kb on + strand at 3514.246 kb on - strand at 3514.257 kb on + strand, within Pf6N2E2_3030 at 3514.258 kb on - strand, within Pf6N2E2_3030 at 3514.258 kb on - strand, within Pf6N2E2_3030 at 3514.258 kb on - strand, within Pf6N2E2_3030 at 3514.263 kb on + strand, within Pf6N2E2_3030 at 3514.264 kb on - strand, within Pf6N2E2_3030 at 3514.264 kb on - strand, within Pf6N2E2_3030 at 3514.264 kb on - strand, within Pf6N2E2_3030 at 3514.264 kb on - strand, within Pf6N2E2_3030 at 3514.264 kb on - strand, within Pf6N2E2_3030 at 3514.315 kb on - strand, within Pf6N2E2_3030 at 3514.343 kb on + strand, within Pf6N2E2_3030 at 3514.343 kb on + strand, within Pf6N2E2_3030 at 3514.343 kb on + strand, within Pf6N2E2_3030 at 3514.348 kb on + strand, within Pf6N2E2_3030 at 3514.349 kb on - strand, within Pf6N2E2_3030 at 3514.349 kb on - strand, within Pf6N2E2_3030 at 3514.481 kb on - strand, within Pf6N2E2_3030 at 3514.481 kb on - strand, within Pf6N2E2_3030 at 3514.539 kb on + strand, within Pf6N2E2_3030 at 3514.539 kb on + strand, within Pf6N2E2_3030 at 3514.539 kb on + strand, within Pf6N2E2_3030 at 3514.539 kb on + strand, within Pf6N2E2_3030 at 3514.539 kb on + strand, within Pf6N2E2_3030 at 3514.540 kb on - strand, within Pf6N2E2_3030 at 3514.644 kb on - strand at 3514.659 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.5 mM remove 3,512,366 + Pf6N2E2_3026 0.39 +1.2 3,512,367 - Pf6N2E2_3026 0.39 +0.8 3,512,453 + Pf6N2E2_3026 0.65 -0.6 3,512,453 + Pf6N2E2_3026 0.65 +0.1 3,512,454 - Pf6N2E2_3026 0.65 +2.1 3,512,454 - Pf6N2E2_3026 0.65 +0.3 3,512,454 - Pf6N2E2_3026 0.65 +0.1 3,512,454 - Pf6N2E2_3026 0.65 +0.7 3,512,454 - Pf6N2E2_3026 0.65 +0.6 3,512,512 + Pf6N2E2_3026 0.83 -0.8 3,512,512 + Pf6N2E2_3026 0.83 -0.1 3,512,548 - -0.2 3,512,582 - +0.1 3,512,721 - Pf6N2E2_3027 0.23 -0.3 3,513,214 + -1.2 3,513,214 + -0.2 3,513,214 + -0.4 3,513,240 + +0.5 3,513,252 - Pf6N2E2_3028 0.11 -0.8 3,513,305 - Pf6N2E2_3028 0.21 -0.5 3,513,340 + Pf6N2E2_3028 0.28 +0.6 3,513,341 - Pf6N2E2_3028 0.28 -1.6 3,513,341 - Pf6N2E2_3028 0.28 +0.0 3,513,341 - Pf6N2E2_3028 0.28 -0.1 3,513,341 - Pf6N2E2_3028 0.28 -0.4 3,513,640 + Pf6N2E2_3028 0.87 -0.9 3,513,641 - Pf6N2E2_3028 0.87 -0.5 3,513,724 + -0.0 3,513,724 + +1.1 3,513,725 - -0.1 3,513,901 + Pf6N2E2_3029 0.45 +0.5 3,513,901 + Pf6N2E2_3029 0.45 -1.0 3,513,901 + Pf6N2E2_3029 0.45 +0.4 3,513,901 + Pf6N2E2_3029 0.45 -0.7 3,513,901 + Pf6N2E2_3029 0.45 -0.9 3,513,901 + Pf6N2E2_3029 0.45 -0.4 3,513,901 + Pf6N2E2_3029 0.45 +0.0 3,513,902 - Pf6N2E2_3029 0.45 +2.1 3,513,902 - Pf6N2E2_3029 0.45 -0.1 3,514,116 + +1.3 3,514,117 - -0.5 3,514,136 - -0.7 3,514,163 - +0.9 3,514,192 - -0.2 3,514,192 - -0.4 3,514,215 + +0.1 3,514,215 + +1.1 3,514,216 - +0.9 3,514,216 - +1.7 3,514,216 - +0.6 3,514,216 - +1.1 3,514,245 + -0.1 3,514,245 + +1.1 3,514,245 + +0.7 3,514,245 + +0.4 3,514,245 + +1.1 3,514,246 - +0.6 3,514,257 + Pf6N2E2_3030 0.10 +0.4 3,514,258 - Pf6N2E2_3030 0.10 +0.1 3,514,258 - Pf6N2E2_3030 0.10 +0.5 3,514,258 - Pf6N2E2_3030 0.10 -1.3 3,514,263 + Pf6N2E2_3030 0.11 -1.0 3,514,264 - Pf6N2E2_3030 0.12 -0.5 3,514,264 - Pf6N2E2_3030 0.12 +1.1 3,514,264 - Pf6N2E2_3030 0.12 -0.5 3,514,264 - Pf6N2E2_3030 0.12 -0.5 3,514,264 - Pf6N2E2_3030 0.12 +0.3 3,514,315 - Pf6N2E2_3030 0.24 -0.2 3,514,343 + Pf6N2E2_3030 0.30 +0.4 3,514,343 + Pf6N2E2_3030 0.30 -0.3 3,514,343 + Pf6N2E2_3030 0.30 +0.0 3,514,348 + Pf6N2E2_3030 0.31 +0.8 3,514,349 - Pf6N2E2_3030 0.31 -1.0 3,514,349 - Pf6N2E2_3030 0.31 -0.3 3,514,481 - Pf6N2E2_3030 0.62 +1.1 3,514,481 - Pf6N2E2_3030 0.62 +0.2 3,514,539 + Pf6N2E2_3030 0.76 +0.7 3,514,539 + Pf6N2E2_3030 0.76 +0.8 3,514,539 + Pf6N2E2_3030 0.76 +0.6 3,514,539 + Pf6N2E2_3030 0.76 -0.0 3,514,539 + Pf6N2E2_3030 0.76 +0.9 3,514,540 - Pf6N2E2_3030 0.76 +0.3 3,514,644 - +0.1 3,514,659 - +0.0
Or see this region's nucleotide sequence