Experiment: Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1419 and Pf6N2E2_1420 are separated by 2 nucleotides Pf6N2E2_1420 and Pf6N2E2_1421 overlap by 8 nucleotides
Pf6N2E2_1419 - Type I secretion outer membrane protein, TolC precursor, at 1,665,754 to 1,667,103
_1419
Pf6N2E2_1420 - ABC exporter for hemopore HasA, membrane fusion protein (MFP) family component HasE, at 1,667,106 to 1,668,476
_1420
Pf6N2E2_1421 - ABC-type protease exporter, ATP-binding component PrtD/AprD, at 1,668,469 to 1,670,190
_1421
Position (kb)
1667
1668
1669 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1666.137 kb on - strand, within Pf6N2E2_1419 at 1666.137 kb on - strand, within Pf6N2E2_1419 at 1666.166 kb on + strand, within Pf6N2E2_1419 at 1666.166 kb on + strand, within Pf6N2E2_1419 at 1666.167 kb on - strand, within Pf6N2E2_1419 at 1666.167 kb on - strand, within Pf6N2E2_1419 at 1666.167 kb on - strand, within Pf6N2E2_1419 at 1666.167 kb on - strand, within Pf6N2E2_1419 at 1666.169 kb on + strand, within Pf6N2E2_1419 at 1666.169 kb on + strand, within Pf6N2E2_1419 at 1666.169 kb on + strand, within Pf6N2E2_1419 at 1666.169 kb on + strand, within Pf6N2E2_1419 at 1666.170 kb on - strand at 1666.170 kb on - strand, within Pf6N2E2_1419 at 1666.357 kb on + strand at 1666.358 kb on - strand, within Pf6N2E2_1419 at 1666.573 kb on - strand, within Pf6N2E2_1419 at 1666.655 kb on + strand, within Pf6N2E2_1419 at 1666.656 kb on - strand, within Pf6N2E2_1419 at 1666.676 kb on + strand, within Pf6N2E2_1419 at 1666.826 kb on + strand, within Pf6N2E2_1419 at 1666.827 kb on - strand, within Pf6N2E2_1419 at 1667.055 kb on + strand at 1667.058 kb on - strand at 1667.159 kb on - strand at 1667.159 kb on - strand at 1667.358 kb on - strand, within Pf6N2E2_1420 at 1667.517 kb on - strand, within Pf6N2E2_1420 at 1667.608 kb on + strand, within Pf6N2E2_1420 at 1667.609 kb on - strand, within Pf6N2E2_1420 at 1667.872 kb on + strand, within Pf6N2E2_1420 at 1667.872 kb on + strand, within Pf6N2E2_1420 at 1667.872 kb on + strand, within Pf6N2E2_1420 at 1667.894 kb on - strand, within Pf6N2E2_1420 at 1668.445 kb on + strand at 1668.446 kb on - strand at 1668.446 kb on - strand at 1668.446 kb on - strand at 1668.604 kb on + strand at 1668.604 kb on + strand at 1668.604 kb on + strand at 1668.604 kb on + strand at 1668.605 kb on - strand at 1668.839 kb on - strand, within Pf6N2E2_1421 at 1668.972 kb on - strand, within Pf6N2E2_1421 at 1669.181 kb on + strand, within Pf6N2E2_1421 at 1669.181 kb on + strand, within Pf6N2E2_1421 at 1669.181 kb on + strand, within Pf6N2E2_1421 at 1669.181 kb on + strand, within Pf6N2E2_1421 at 1669.182 kb on - strand, within Pf6N2E2_1421 at 1669.182 kb on - strand, within Pf6N2E2_1421 at 1669.182 kb on - strand, within Pf6N2E2_1421 at 1669.182 kb on - strand, within Pf6N2E2_1421 at 1669.182 kb on - strand, within Pf6N2E2_1421 at 1669.182 kb on - strand, within Pf6N2E2_1421 at 1669.182 kb on - strand, within Pf6N2E2_1421 at 1669.278 kb on - strand, within Pf6N2E2_1421 at 1669.467 kb on + strand, within Pf6N2E2_1421 at 1669.468 kb on - strand, within Pf6N2E2_1421 at 1669.470 kb on + strand, within Pf6N2E2_1421 at 1669.471 kb on - strand, within Pf6N2E2_1421 at 1669.471 kb on - strand, within Pf6N2E2_1421 at 1669.471 kb on - strand, within Pf6N2E2_1421 at 1669.471 kb on - strand, within Pf6N2E2_1421
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.5 mM remove 1,666,137 - Pf6N2E2_1419 0.28 +0.7 1,666,137 - Pf6N2E2_1419 0.28 -0.1 1,666,166 + Pf6N2E2_1419 0.31 +1.0 1,666,166 + Pf6N2E2_1419 0.31 +0.8 1,666,167 - Pf6N2E2_1419 0.31 +0.2 1,666,167 - Pf6N2E2_1419 0.31 -1.4 1,666,167 - Pf6N2E2_1419 0.31 +2.6 1,666,167 - Pf6N2E2_1419 0.31 +0.4 1,666,169 + Pf6N2E2_1419 0.31 -0.5 1,666,169 + Pf6N2E2_1419 0.31 +0.6 1,666,169 + Pf6N2E2_1419 0.31 -0.2 1,666,169 + Pf6N2E2_1419 0.31 +1.5 1,666,170 - +1.4 1,666,170 - Pf6N2E2_1419 0.31 +0.3 1,666,357 + +2.6 1,666,358 - Pf6N2E2_1419 0.45 +0.0 1,666,573 - Pf6N2E2_1419 0.61 -1.5 1,666,655 + Pf6N2E2_1419 0.67 +0.9 1,666,656 - Pf6N2E2_1419 0.67 -1.5 1,666,676 + Pf6N2E2_1419 0.68 +0.4 1,666,826 + Pf6N2E2_1419 0.79 +0.3 1,666,827 - Pf6N2E2_1419 0.79 -0.4 1,667,055 + -0.6 1,667,058 - -0.4 1,667,159 - +0.6 1,667,159 - -0.7 1,667,358 - Pf6N2E2_1420 0.18 -0.1 1,667,517 - Pf6N2E2_1420 0.30 -0.5 1,667,608 + Pf6N2E2_1420 0.37 -1.0 1,667,609 - Pf6N2E2_1420 0.37 -1.6 1,667,872 + Pf6N2E2_1420 0.56 +0.5 1,667,872 + Pf6N2E2_1420 0.56 -0.5 1,667,872 + Pf6N2E2_1420 0.56 -0.6 1,667,894 - Pf6N2E2_1420 0.57 -0.1 1,668,445 + +0.5 1,668,446 - +0.3 1,668,446 - -0.4 1,668,446 - -0.2 1,668,604 + +0.7 1,668,604 + +0.7 1,668,604 + +0.8 1,668,604 + +1.0 1,668,605 - +0.6 1,668,839 - Pf6N2E2_1421 0.21 -0.3 1,668,972 - Pf6N2E2_1421 0.29 +0.2 1,669,181 + Pf6N2E2_1421 0.41 +0.2 1,669,181 + Pf6N2E2_1421 0.41 -1.4 1,669,181 + Pf6N2E2_1421 0.41 +0.2 1,669,181 + Pf6N2E2_1421 0.41 -0.8 1,669,182 - Pf6N2E2_1421 0.41 +0.1 1,669,182 - Pf6N2E2_1421 0.41 +0.4 1,669,182 - Pf6N2E2_1421 0.41 +0.1 1,669,182 - Pf6N2E2_1421 0.41 -0.1 1,669,182 - Pf6N2E2_1421 0.41 -0.7 1,669,182 - Pf6N2E2_1421 0.41 -1.8 1,669,182 - Pf6N2E2_1421 0.41 -0.1 1,669,278 - Pf6N2E2_1421 0.47 -0.5 1,669,467 + Pf6N2E2_1421 0.58 +0.4 1,669,468 - Pf6N2E2_1421 0.58 -0.4 1,669,470 + Pf6N2E2_1421 0.58 -1.1 1,669,471 - Pf6N2E2_1421 0.58 -1.0 1,669,471 - Pf6N2E2_1421 0.58 +0.8 1,669,471 - Pf6N2E2_1421 0.58 +1.6 1,669,471 - Pf6N2E2_1421 0.58 +1.2
Or see this region's nucleotide sequence