Experiment: Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1288 and Pf6N2E2_1289 overlap by 4 nucleotides Pf6N2E2_1289 and Pf6N2E2_1290 are separated by 3 nucleotides
Pf6N2E2_1288 - Protein containing transglutaminase-like domain, putative cysteine protease, at 1,518,857 to 1,519,654
_1288
Pf6N2E2_1289 - Protein containing domains DUF403, at 1,519,651 to 1,520,601
_1289
Pf6N2E2_1290 - Protein containing domains DUF404, DUF407, at 1,520,605 to 1,522,014
_1290
Position (kb)
1519
1520
1521 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1518.810 kb on - strand at 1518.858 kb on + strand at 1518.859 kb on - strand at 1519.030 kb on - strand, within Pf6N2E2_1288 at 1519.101 kb on + strand, within Pf6N2E2_1288 at 1519.113 kb on + strand, within Pf6N2E2_1288 at 1519.617 kb on + strand at 1519.617 kb on + strand at 1519.617 kb on + strand at 1519.617 kb on + strand at 1519.617 kb on + strand at 1519.618 kb on - strand at 1519.618 kb on - strand at 1519.623 kb on + strand at 1519.623 kb on + strand at 1519.623 kb on + strand at 1519.654 kb on + strand at 1519.655 kb on - strand at 1519.667 kb on + strand at 1519.667 kb on + strand at 1519.667 kb on + strand at 1520.055 kb on - strand, within Pf6N2E2_1289 at 1520.069 kb on - strand, within Pf6N2E2_1289 at 1520.123 kb on + strand, within Pf6N2E2_1289 at 1520.123 kb on + strand, within Pf6N2E2_1289 at 1520.342 kb on + strand, within Pf6N2E2_1289 at 1520.342 kb on + strand, within Pf6N2E2_1289 at 1520.343 kb on - strand, within Pf6N2E2_1289 at 1520.343 kb on - strand, within Pf6N2E2_1289 at 1520.343 kb on - strand, within Pf6N2E2_1289 at 1520.343 kb on - strand, within Pf6N2E2_1289 at 1520.346 kb on + strand, within Pf6N2E2_1289 at 1520.347 kb on - strand, within Pf6N2E2_1289 at 1520.347 kb on - strand, within Pf6N2E2_1289 at 1520.423 kb on + strand, within Pf6N2E2_1289 at 1520.423 kb on + strand, within Pf6N2E2_1289 at 1520.514 kb on - strand at 1520.606 kb on + strand at 1520.606 kb on + strand at 1520.606 kb on + strand at 1520.606 kb on + strand at 1520.606 kb on + strand at 1520.607 kb on - strand at 1520.607 kb on - strand at 1520.617 kb on + strand at 1520.822 kb on + strand, within Pf6N2E2_1290 at 1520.822 kb on + strand, within Pf6N2E2_1290 at 1520.822 kb on + strand, within Pf6N2E2_1290 at 1520.822 kb on + strand, within Pf6N2E2_1290 at 1520.823 kb on - strand, within Pf6N2E2_1290 at 1521.029 kb on + strand, within Pf6N2E2_1290 at 1521.029 kb on + strand, within Pf6N2E2_1290 at 1521.030 kb on - strand, within Pf6N2E2_1290 at 1521.053 kb on + strand, within Pf6N2E2_1290 at 1521.059 kb on + strand, within Pf6N2E2_1290 at 1521.059 kb on + strand, within Pf6N2E2_1290 at 1521.060 kb on - strand, within Pf6N2E2_1290 at 1521.188 kb on + strand, within Pf6N2E2_1290 at 1521.210 kb on - strand, within Pf6N2E2_1290 at 1521.210 kb on - strand, within Pf6N2E2_1290 at 1521.251 kb on + strand, within Pf6N2E2_1290 at 1521.251 kb on + strand, within Pf6N2E2_1290 at 1521.251 kb on + strand, within Pf6N2E2_1290 at 1521.252 kb on - strand, within Pf6N2E2_1290 at 1521.252 kb on - strand, within Pf6N2E2_1290 at 1521.252 kb on - strand, within Pf6N2E2_1290 at 1521.252 kb on - strand, within Pf6N2E2_1290 at 1521.252 kb on - strand, within Pf6N2E2_1290 at 1521.252 kb on - strand, within Pf6N2E2_1290 at 1521.335 kb on + strand, within Pf6N2E2_1290 at 1521.336 kb on - strand, within Pf6N2E2_1290 at 1521.509 kb on + strand, within Pf6N2E2_1290 at 1521.509 kb on + strand, within Pf6N2E2_1290 at 1521.509 kb on + strand, within Pf6N2E2_1290 at 1521.551 kb on + strand, within Pf6N2E2_1290 at 1521.551 kb on + strand, within Pf6N2E2_1290 at 1521.551 kb on + strand, within Pf6N2E2_1290 at 1521.551 kb on + strand, within Pf6N2E2_1290 at 1521.551 kb on + strand, within Pf6N2E2_1290 at 1521.552 kb on - strand, within Pf6N2E2_1290 at 1521.552 kb on - strand, within Pf6N2E2_1290 at 1521.554 kb on + strand, within Pf6N2E2_1290 at 1521.554 kb on + strand, within Pf6N2E2_1290 at 1521.555 kb on - strand, within Pf6N2E2_1290 at 1521.595 kb on - strand, within Pf6N2E2_1290
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.5 mM remove 1,518,810 - -1.1 1,518,858 + +0.0 1,518,859 - +1.6 1,519,030 - Pf6N2E2_1288 0.22 -0.1 1,519,101 + Pf6N2E2_1288 0.31 +0.6 1,519,113 + Pf6N2E2_1288 0.32 +1.4 1,519,617 + +0.7 1,519,617 + -0.2 1,519,617 + +0.1 1,519,617 + -0.3 1,519,617 + -0.5 1,519,618 - +0.2 1,519,618 - +0.9 1,519,623 + -1.5 1,519,623 + -0.6 1,519,623 + +0.2 1,519,654 + -0.3 1,519,655 - -0.4 1,519,667 + +0.2 1,519,667 + +0.5 1,519,667 + -0.1 1,520,055 - Pf6N2E2_1289 0.42 -1.6 1,520,069 - Pf6N2E2_1289 0.44 +0.2 1,520,123 + Pf6N2E2_1289 0.50 -0.7 1,520,123 + Pf6N2E2_1289 0.50 -0.7 1,520,342 + Pf6N2E2_1289 0.73 -1.0 1,520,342 + Pf6N2E2_1289 0.73 -0.7 1,520,343 - Pf6N2E2_1289 0.73 -2.0 1,520,343 - Pf6N2E2_1289 0.73 +1.4 1,520,343 - Pf6N2E2_1289 0.73 -0.7 1,520,343 - Pf6N2E2_1289 0.73 -0.4 1,520,346 + Pf6N2E2_1289 0.73 -1.1 1,520,347 - Pf6N2E2_1289 0.73 +0.8 1,520,347 - Pf6N2E2_1289 0.73 -0.4 1,520,423 + Pf6N2E2_1289 0.81 +0.8 1,520,423 + Pf6N2E2_1289 0.81 -0.6 1,520,514 - +0.0 1,520,606 + -0.3 1,520,606 + -0.9 1,520,606 + +0.6 1,520,606 + +0.5 1,520,606 + -0.2 1,520,607 - +0.2 1,520,607 - +0.5 1,520,617 + -0.8 1,520,822 + Pf6N2E2_1290 0.15 -0.7 1,520,822 + Pf6N2E2_1290 0.15 +2.6 1,520,822 + Pf6N2E2_1290 0.15 +0.6 1,520,822 + Pf6N2E2_1290 0.15 +0.2 1,520,823 - Pf6N2E2_1290 0.15 -0.4 1,521,029 + Pf6N2E2_1290 0.30 -0.3 1,521,029 + Pf6N2E2_1290 0.30 +1.0 1,521,030 - Pf6N2E2_1290 0.30 -0.6 1,521,053 + Pf6N2E2_1290 0.32 +0.2 1,521,059 + Pf6N2E2_1290 0.32 -0.3 1,521,059 + Pf6N2E2_1290 0.32 -0.9 1,521,060 - Pf6N2E2_1290 0.32 +0.3 1,521,188 + Pf6N2E2_1290 0.41 +0.4 1,521,210 - Pf6N2E2_1290 0.43 -0.4 1,521,210 - Pf6N2E2_1290 0.43 +0.3 1,521,251 + Pf6N2E2_1290 0.46 +0.6 1,521,251 + Pf6N2E2_1290 0.46 -0.2 1,521,251 + Pf6N2E2_1290 0.46 -0.7 1,521,252 - Pf6N2E2_1290 0.46 -0.1 1,521,252 - Pf6N2E2_1290 0.46 +1.4 1,521,252 - Pf6N2E2_1290 0.46 -0.2 1,521,252 - Pf6N2E2_1290 0.46 -0.7 1,521,252 - Pf6N2E2_1290 0.46 -0.5 1,521,252 - Pf6N2E2_1290 0.46 +0.2 1,521,335 + Pf6N2E2_1290 0.52 -1.3 1,521,336 - Pf6N2E2_1290 0.52 -0.2 1,521,509 + Pf6N2E2_1290 0.64 +0.4 1,521,509 + Pf6N2E2_1290 0.64 +0.8 1,521,509 + Pf6N2E2_1290 0.64 -0.8 1,521,551 + Pf6N2E2_1290 0.67 -0.2 1,521,551 + Pf6N2E2_1290 0.67 -0.0 1,521,551 + Pf6N2E2_1290 0.67 -0.6 1,521,551 + Pf6N2E2_1290 0.67 +0.2 1,521,551 + Pf6N2E2_1290 0.67 +0.7 1,521,552 - Pf6N2E2_1290 0.67 +0.3 1,521,552 - Pf6N2E2_1290 0.67 -0.6 1,521,554 + Pf6N2E2_1290 0.67 +0.7 1,521,554 + Pf6N2E2_1290 0.67 -2.1 1,521,555 - Pf6N2E2_1290 0.67 +1.9 1,521,595 - Pf6N2E2_1290 0.70 -1.3
Or see this region's nucleotide sequence