Experiment: Varel_Bryant medium with Chlorpromazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4701 and BT4702 are separated by 3 nucleotides BT4702 and BT4703 are separated by 3 nucleotides
BT4701: BT4701 - 2-oxoglutarate decarboxylase and 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (NCBI ptt file), at 6,170,350 to 6,172,017
BT4701
BT4702: BT4702 - dihydroxynapthoic acid synthetase (NCBI ptt file), at 6,172,021 to 6,172,845
BT4702
BT4703: BT4703 - chloromuconate cycloisomerase (NCBI ptt file), at 6,172,849 to 6,173,874
BT4703
Position (kb)
6172
6173 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6172.020 kb on + strand at 6172.078 kb on + strand at 6172.319 kb on + strand, within BT4702 at 6172.535 kb on + strand, within BT4702 at 6172.593 kb on + strand, within BT4702 at 6172.599 kb on - strand, within BT4702 at 6172.756 kb on + strand, within BT4702 at 6172.760 kb on - strand, within BT4702 at 6172.809 kb on + strand at 6173.053 kb on - strand, within BT4703 at 6173.184 kb on + strand, within BT4703 at 6173.211 kb on + strand, within BT4703 at 6173.385 kb on - strand, within BT4703 at 6173.465 kb on + strand, within BT4703 at 6173.521 kb on - strand, within BT4703 at 6173.565 kb on - strand, within BT4703 at 6173.570 kb on - strand, within BT4703 at 6173.571 kb on + strand, within BT4703 at 6173.574 kb on + strand, within BT4703 at 6173.575 kb on - strand, within BT4703 at 6173.626 kb on - strand, within BT4703 at 6173.628 kb on + strand, within BT4703 at 6173.711 kb on + strand, within BT4703 at 6173.807 kb on + strand at 6173.820 kb on + strand at 6173.825 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.0125 mM remove 6,172,020 + +1.0 6,172,078 + +1.6 6,172,319 + BT4702 0.36 -1.5 6,172,535 + BT4702 0.62 +0.7 6,172,593 + BT4702 0.69 -0.0 6,172,599 - BT4702 0.70 +0.8 6,172,756 + BT4702 0.89 -0.0 6,172,760 - BT4702 0.90 +2.6 6,172,809 + -0.5 6,173,053 - BT4703 0.20 +1.0 6,173,184 + BT4703 0.33 -0.5 6,173,211 + BT4703 0.35 +1.0 6,173,385 - BT4703 0.52 -1.6 6,173,465 + BT4703 0.60 +0.2 6,173,521 - BT4703 0.65 -1.4 6,173,565 - BT4703 0.70 +0.6 6,173,570 - BT4703 0.70 +1.0 6,173,571 + BT4703 0.70 +2.0 6,173,574 + BT4703 0.71 -2.2 6,173,575 - BT4703 0.71 -0.9 6,173,626 - BT4703 0.76 +0.5 6,173,628 + BT4703 0.76 +0.4 6,173,711 + BT4703 0.84 +1.0 6,173,807 + +1.0 6,173,820 + -0.0 6,173,825 + -0.6
Or see this region's nucleotide sequence