Experiment: Varel_Bryant medium with Chlorpromazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4440 and BT4441 are separated by 16 nucleotides BT4441 and BT4442 are separated by 227 nucleotides BT4442 and BT4443 overlap by 47 nucleotides
BT4440: BT4440 - conserved hypothetical protein, putative cell surface protein (NCBI ptt file), at 5,846,629 to 5,853,180
BT4440
BT4441: BT4441 - cell surface protein (NCBI ptt file), at 5,853,197 to 5,853,922
BT4441
BT4442: BT4442 - hypothetical protein (NCBI ptt file), at 5,854,150 to 5,854,500
BT4442
BT4443: BT4443 - N-acetylmuramoyl alanine amidase (NCBI ptt file), at 5,854,454 to 5,855,110
BT4443
Position (kb) 5853
5854 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5852.209 kb on - strand, within BT4440 at 5852.337 kb on - strand, within BT4440 at 5852.392 kb on - strand, within BT4440 at 5852.417 kb on + strand, within BT4440 at 5852.417 kb on + strand, within BT4440 at 5852.421 kb on + strand, within BT4440 at 5852.431 kb on - strand, within BT4440 at 5852.443 kb on - strand, within BT4440 at 5852.465 kb on - strand, within BT4440 at 5852.488 kb on - strand, within BT4440 at 5852.538 kb on - strand at 5852.680 kb on - strand at 5852.710 kb on + strand at 5852.730 kb on + strand at 5852.731 kb on - strand at 5852.731 kb on - strand at 5852.843 kb on + strand at 5852.889 kb on + strand at 5852.930 kb on + strand at 5852.987 kb on + strand at 5852.990 kb on + strand at 5852.995 kb on - strand at 5853.204 kb on - strand at 5853.305 kb on + strand, within BT4441 at 5853.380 kb on - strand, within BT4441 at 5853.444 kb on - strand, within BT4441 at 5853.464 kb on + strand, within BT4441 at 5853.494 kb on + strand at 5853.495 kb on - strand, within BT4441 at 5853.521 kb on - strand, within BT4441 at 5853.521 kb on - strand, within BT4441 at 5853.521 kb on - strand, within BT4441 at 5853.745 kb on - strand, within BT4441 at 5853.808 kb on - strand, within BT4441 at 5853.864 kb on - strand at 5854.015 kb on - strand at 5854.050 kb on - strand at 5854.095 kb on - strand at 5854.170 kb on - strand at 5854.225 kb on + strand, within BT4442 at 5854.324 kb on - strand, within BT4442 at 5854.353 kb on + strand, within BT4442 at 5854.353 kb on + strand, within BT4442 at 5854.353 kb on + strand, within BT4442 at 5854.424 kb on + strand, within BT4442 at 5854.534 kb on - strand, within BT4443 at 5854.534 kb on - strand, within BT4443 at 5854.537 kb on + strand, within BT4443 at 5854.604 kb on - strand, within BT4443 at 5854.666 kb on + strand, within BT4443 at 5854.690 kb on - strand, within BT4443 at 5854.690 kb on - strand, within BT4443 at 5854.690 kb on - strand, within BT4443 at 5854.690 kb on - strand, within BT4443 at 5854.701 kb on + strand, within BT4443 at 5854.701 kb on + strand, within BT4443 at 5854.789 kb on + strand at 5854.800 kb on + strand, within BT4443 at 5854.852 kb on + strand, within BT4443 at 5854.853 kb on - strand, within BT4443
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.0125 mM remove 5,852,209 - BT4440 0.85 +0.4 5,852,337 - BT4440 0.87 -1.1 5,852,392 - BT4440 0.88 +2.0 5,852,417 + BT4440 0.88 -0.8 5,852,417 + BT4440 0.88 -2.0 5,852,421 + BT4440 0.88 -0.0 5,852,431 - BT4440 0.89 -0.0 5,852,443 - BT4440 0.89 -0.6 5,852,465 - BT4440 0.89 -0.0 5,852,488 - BT4440 0.89 -1.8 5,852,538 - -1.6 5,852,680 - -1.4 5,852,710 + +1.0 5,852,730 + -0.4 5,852,731 - -2.5 5,852,731 - -2.0 5,852,843 + -1.0 5,852,889 + -0.3 5,852,930 + -0.0 5,852,987 + -1.0 5,852,990 + -1.6 5,852,995 - -0.0 5,853,204 - +2.0 5,853,305 + BT4441 0.15 -0.5 5,853,380 - BT4441 0.25 -1.0 5,853,444 - BT4441 0.34 -2.2 5,853,464 + BT4441 0.37 -1.0 5,853,494 + +2.0 5,853,495 - BT4441 0.41 -0.8 5,853,521 - BT4441 0.45 -2.3 5,853,521 - BT4441 0.45 -1.5 5,853,521 - BT4441 0.45 -1.2 5,853,745 - BT4441 0.75 -2.6 5,853,808 - BT4441 0.84 -1.6 5,853,864 - -0.7 5,854,015 - +0.4 5,854,050 - -2.2 5,854,095 - -1.7 5,854,170 - -0.0 5,854,225 + BT4442 0.21 -1.9 5,854,324 - BT4442 0.50 +1.0 5,854,353 + BT4442 0.58 -0.6 5,854,353 + BT4442 0.58 -2.1 5,854,353 + BT4442 0.58 -2.5 5,854,424 + BT4442 0.78 -2.2 5,854,534 - BT4443 0.12 -1.2 5,854,534 - BT4443 0.12 -0.6 5,854,537 + BT4443 0.13 -0.9 5,854,604 - BT4443 0.23 -1.3 5,854,666 + BT4443 0.32 -0.2 5,854,690 - BT4443 0.36 +1.0 5,854,690 - BT4443 0.36 +1.0 5,854,690 - BT4443 0.36 -0.3 5,854,690 - BT4443 0.36 +2.0 5,854,701 + BT4443 0.38 -0.7 5,854,701 + BT4443 0.38 +1.0 5,854,789 + -0.6 5,854,800 + BT4443 0.53 -0.8 5,854,852 + BT4443 0.61 +0.5 5,854,853 - BT4443 0.61 -1.5
Or see this region's nucleotide sequence