Experiment: Varel_Bryant medium with Chlorpromazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3881 and BT3882 are separated by 88 nucleotides BT3882 and BT3883 overlap by 1 nucleotides BT3883 and BT3884 are separated by 39 nucleotides
BT3881: BT3881 - hypothetical protein (NCBI ptt file), at 5,052,952 to 5,054,124
BT3881
BT3882: BT3882 - putative DNA binding protein (NCBI ptt file), at 5,054,213 to 5,054,875
BT3882
BT3883: BT3883 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (NCBI ptt file), at 5,054,875 to 5,055,489
BT3883
BT3884: BT3884 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (NCBI ptt file), at 5,055,529 to 5,056,008
BT3884
Position (kb) 5054
5055 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5053.231 kb on + strand, within BT3881 at 5053.232 kb on - strand, within BT3881 at 5053.234 kb on - strand, within BT3881 at 5053.262 kb on + strand, within BT3881 at 5053.413 kb on + strand, within BT3881 at 5053.416 kb on - strand, within BT3881 at 5053.416 kb on - strand, within BT3881 at 5053.497 kb on - strand, within BT3881 at 5053.524 kb on + strand, within BT3881 at 5053.524 kb on + strand, within BT3881 at 5053.538 kb on - strand, within BT3881 at 5053.549 kb on + strand, within BT3881 at 5053.567 kb on + strand, within BT3881 at 5053.567 kb on + strand, within BT3881 at 5053.593 kb on + strand, within BT3881 at 5053.594 kb on - strand, within BT3881 at 5053.683 kb on - strand, within BT3881 at 5053.698 kb on + strand, within BT3881 at 5053.836 kb on + strand, within BT3881 at 5053.840 kb on + strand, within BT3881 at 5053.846 kb on + strand, within BT3881 at 5053.847 kb on - strand, within BT3881 at 5053.847 kb on - strand, within BT3881 at 5053.875 kb on + strand, within BT3881 at 5053.879 kb on - strand, within BT3881 at 5054.122 kb on + strand at 5054.142 kb on + strand at 5054.205 kb on + strand at 5054.232 kb on + strand at 5054.248 kb on + strand at 5054.353 kb on + strand, within BT3882 at 5054.399 kb on + strand, within BT3882 at 5054.419 kb on + strand, within BT3882 at 5054.479 kb on + strand, within BT3882 at 5054.589 kb on + strand, within BT3882 at 5054.633 kb on + strand, within BT3882 at 5054.812 kb on + strand at 5054.873 kb on + strand at 5054.873 kb on + strand at 5054.902 kb on + strand at 5054.931 kb on + strand at 5054.943 kb on + strand, within BT3883 at 5055.053 kb on + strand, within BT3883 at 5055.053 kb on + strand, within BT3883 at 5055.244 kb on + strand, within BT3883 at 5055.478 kb on + strand at 5055.478 kb on + strand at 5055.482 kb on + strand at 5055.482 kb on + strand at 5055.482 kb on + strand at 5055.496 kb on + strand at 5055.503 kb on + strand at 5055.528 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.0125 mM remove 5,053,231 + BT3881 0.24 -0.2 5,053,232 - BT3881 0.24 -2.0 5,053,234 - BT3881 0.24 -0.8 5,053,262 + BT3881 0.26 -0.4 5,053,413 + BT3881 0.39 +1.0 5,053,416 - BT3881 0.40 +1.0 5,053,416 - BT3881 0.40 +0.0 5,053,497 - BT3881 0.46 -2.8 5,053,524 + BT3881 0.49 +1.4 5,053,524 + BT3881 0.49 +0.1 5,053,538 - BT3881 0.50 -1.0 5,053,549 + BT3881 0.51 +0.4 5,053,567 + BT3881 0.52 -0.6 5,053,567 + BT3881 0.52 +0.2 5,053,593 + BT3881 0.55 -0.4 5,053,594 - BT3881 0.55 +0.8 5,053,683 - BT3881 0.62 -0.7 5,053,698 + BT3881 0.64 -2.4 5,053,836 + BT3881 0.75 -0.3 5,053,840 + BT3881 0.76 +2.0 5,053,846 + BT3881 0.76 -1.9 5,053,847 - BT3881 0.76 -0.6 5,053,847 - BT3881 0.76 +0.4 5,053,875 + BT3881 0.79 -0.6 5,053,879 - BT3881 0.79 -2.7 5,054,122 + -0.8 5,054,142 + +0.0 5,054,205 + -1.6 5,054,232 + -0.4 5,054,248 + +0.3 5,054,353 + BT3882 0.21 +0.4 5,054,399 + BT3882 0.28 -1.3 5,054,419 + BT3882 0.31 +2.0 5,054,479 + BT3882 0.40 -0.4 5,054,589 + BT3882 0.57 -0.6 5,054,633 + BT3882 0.63 +0.7 5,054,812 + -1.4 5,054,873 + +0.0 5,054,873 + +0.4 5,054,902 + -0.8 5,054,931 + -1.3 5,054,943 + BT3883 0.11 +1.8 5,055,053 + BT3883 0.29 +1.0 5,055,053 + BT3883 0.29 +0.4 5,055,244 + BT3883 0.60 -1.5 5,055,478 + -0.3 5,055,478 + -2.0 5,055,482 + -0.8 5,055,482 + +0.5 5,055,482 + -0.2 5,055,496 + -1.0 5,055,503 + -0.8 5,055,528 + -0.6
Or see this region's nucleotide sequence