Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT2742

Experiment: Varel_Bryant medium with Chlorpromazine 0.0125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT2740 and tRNA-Thr are separated by 50 nucleotidestRNA-Thr and tRNA-Gly are separated by 12 nucleotidestRNA-Gly and tRNA-Tyr are separated by 25 nucleotidestRNA-Tyr and tRNA-Thr are separated by 155 nucleotidestRNA-Thr and BT2741 are separated by 97 nucleotidesBT2741 and BT2742 are separated by 16 nucleotidesBT2742 and BT2743 are separated by 456 nucleotides BT2740: BT2740 - elongation factor Tu (NCBI ptt file), at 3,393,922 to 3,395,106 BT2740 BT_t45: tRNA-Thr - tRNA-Thr (RefSeq), at 3,395,157 to 3,395,228 tRNA-Thr BT_t46: tRNA-Gly - tRNA-Gly (RefSeq), at 3,395,241 to 3,395,313 tRNA-Gly BT_t47: tRNA-Tyr - tRNA-Tyr (RefSeq), at 3,395,339 to 3,395,424 tRNA-Tyr BT_t48: tRNA-Thr - tRNA-Thr (RefSeq), at 3,395,580 to 3,395,656 tRNA-Thr BT2741: BT2741 - sigma-54 modulation protein (NCBI ptt file), at 3,395,754 to 3,396,053 BT2741 BT2742: BT2742 - integrase, site-specific recombinase (NCBI ptt file), at 3,396,070 to 3,396,951 BT2742 BT2743: BT2743 - putative aminopeptidase (NCBI ptt file), at 3,397,408 to 3,399,189 BT2743 Position (kb) 3396 3397Strain fitness (log2 ratio) -2 -1 0 1 2at 3395.763 kb on - strandat 3395.763 kb on - strandat 3395.838 kb on - strand, within BT2741at 3395.849 kb on + strand, within BT2741at 3395.850 kb on - strand, within BT2741at 3395.921 kb on + strand, within BT2741at 3395.921 kb on + strand, within BT2741at 3395.944 kb on - strand, within BT2741at 3395.944 kb on - strandat 3396.045 kb on - strandat 3396.054 kb on - strandat 3396.054 kb on - strandat 3396.058 kb on - strandat 3396.058 kb on - strandat 3396.223 kb on - strand, within BT2742at 3397.284 kb on - strandat 3397.360 kb on + strandat 3397.361 kb on - strandat 3397.364 kb on - strandat 3397.383 kb on - strandat 3397.399 kb on + strandat 3397.400 kb on - strandat 3397.402 kb on + strandat 3397.421 kb on + strandat 3397.467 kb on + strandat 3397.513 kb on + strandat 3397.514 kb on - strandat 3397.514 kb on - strandat 3397.518 kb on - strandat 3397.518 kb on - strandat 3397.586 kb on + strandat 3397.587 kb on - strand, within BT2743at 3397.593 kb on + strand, within BT2743at 3397.594 kb on - strand, within BT2743at 3397.662 kb on + strand, within BT2743at 3397.662 kb on + strand, within BT2743at 3397.669 kb on + strand, within BT2743at 3397.671 kb on + strand, within BT2743at 3397.672 kb on - strand, within BT2743at 3397.725 kb on - strand, within BT2743at 3397.746 kb on + strand, within BT2743at 3397.747 kb on - strand, within BT2743at 3397.769 kb on + strand, within BT2743at 3397.895 kb on - strand, within BT2743at 3397.906 kb on + strand, within BT2743at 3397.906 kb on + strand, within BT2743

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Per-strain Table

Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.0125 mM
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3,395,763 - -1.8
3,395,763 - +1.0
3,395,838 - BT2741 0.28 -1.0
3,395,849 + BT2741 0.32 -0.0
3,395,850 - BT2741 0.32 +0.2
3,395,921 + BT2741 0.56 -1.7
3,395,921 + BT2741 0.56 -1.8
3,395,944 - BT2741 0.63 +2.0
3,395,944 - +1.0
3,396,045 - -0.3
3,396,054 - +0.2
3,396,054 - +1.0
3,396,058 - -0.1
3,396,058 - +1.0
3,396,223 - BT2742 0.17 +0.1
3,397,284 - -1.5
3,397,360 + +0.7
3,397,361 - -1.2
3,397,364 - +0.7
3,397,383 - -0.5
3,397,399 + -0.5
3,397,400 - -0.2
3,397,402 + -1.3
3,397,421 + -0.0
3,397,467 + -1.3
3,397,513 + +1.0
3,397,514 - +0.4
3,397,514 - -0.6
3,397,518 - -1.8
3,397,518 - -0.0
3,397,586 + +1.7
3,397,587 - BT2743 0.10 +2.0
3,397,593 + BT2743 0.10 -0.0
3,397,594 - BT2743 0.10 +0.2
3,397,662 + BT2743 0.14 -0.0
3,397,662 + BT2743 0.14 -0.0
3,397,669 + BT2743 0.15 -0.8
3,397,671 + BT2743 0.15 -0.3
3,397,672 - BT2743 0.15 +0.2
3,397,725 - BT2743 0.18 -0.2
3,397,746 + BT2743 0.19 +1.0
3,397,747 - BT2743 0.19 +0.3
3,397,769 + BT2743 0.20 -0.6
3,397,895 - BT2743 0.27 -1.8
3,397,906 + BT2743 0.28 -1.3
3,397,906 + BT2743 0.28 -1.0

Or see this region's nucleotide sequence