Experiment: Varel_Bryant medium with Chlorpromazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1647 and BT1648 overlap by 10 nucleotides BT1648 and BT1649 are separated by 6 nucleotides BT1649 and BT1650 are separated by 96 nucleotides
BT1647: BT1647 - conserved hypothetical protein, putative integral membrane protein (NCBI ptt file), at 2,036,422 to 2,037,720
BT1647
BT1648: BT1648 - putative glycosyltransferase (NCBI ptt file), at 2,037,711 to 2,038,703
BT1648
BT1649: BT1649 - conserved hypothetical protein (NCBI ptt file), at 2,038,710 to 2,039,387
BT1649
BT1650: BT1650 - putative teichoic acid biosynthesis protein F (NCBI ptt file), at 2,039,484 to 2,040,641
BT1650
Position (kb)
2038
2039
2040 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2037.740 kb on + strand at 2037.740 kb on + strand at 2037.853 kb on + strand, within BT1648 at 2037.900 kb on + strand, within BT1648 at 2038.036 kb on + strand, within BT1648 at 2038.040 kb on + strand, within BT1648 at 2038.040 kb on + strand, within BT1648 at 2038.040 kb on + strand, within BT1648 at 2038.040 kb on + strand, within BT1648 at 2038.042 kb on + strand, within BT1648 at 2038.066 kb on + strand, within BT1648 at 2038.067 kb on - strand, within BT1648 at 2038.095 kb on + strand, within BT1648 at 2038.116 kb on + strand, within BT1648 at 2038.128 kb on - strand, within BT1648 at 2038.130 kb on + strand, within BT1648 at 2038.131 kb on - strand, within BT1648 at 2038.135 kb on - strand, within BT1648 at 2038.154 kb on + strand, within BT1648 at 2038.170 kb on + strand, within BT1648 at 2038.227 kb on + strand, within BT1648 at 2038.228 kb on - strand, within BT1648 at 2038.279 kb on - strand, within BT1648 at 2038.291 kb on - strand, within BT1648 at 2038.332 kb on + strand, within BT1648 at 2038.362 kb on + strand, within BT1648 at 2038.383 kb on - strand, within BT1648 at 2038.406 kb on + strand, within BT1648 at 2038.414 kb on + strand, within BT1648 at 2038.416 kb on + strand, within BT1648 at 2038.421 kb on + strand, within BT1648 at 2038.421 kb on + strand, within BT1648 at 2038.423 kb on + strand, within BT1648 at 2038.533 kb on + strand, within BT1648 at 2038.626 kb on + strand at 2038.626 kb on + strand at 2038.627 kb on - strand at 2038.640 kb on + strand at 2038.643 kb on - strand at 2038.656 kb on - strand at 2038.755 kb on + strand at 2038.776 kb on + strand at 2038.832 kb on + strand, within BT1649 at 2038.832 kb on + strand, within BT1649 at 2038.981 kb on + strand, within BT1649 at 2038.981 kb on + strand, within BT1649 at 2038.982 kb on - strand, within BT1649 at 2039.005 kb on + strand, within BT1649 at 2039.005 kb on + strand, within BT1649 at 2039.023 kb on + strand, within BT1649 at 2039.034 kb on - strand, within BT1649 at 2039.053 kb on + strand, within BT1649 at 2039.076 kb on + strand, within BT1649 at 2039.077 kb on - strand, within BT1649 at 2039.104 kb on - strand, within BT1649 at 2039.135 kb on + strand, within BT1649 at 2039.135 kb on + strand, within BT1649 at 2039.177 kb on - strand, within BT1649 at 2039.222 kb on + strand, within BT1649 at 2039.222 kb on + strand, within BT1649 at 2039.324 kb on + strand at 2039.390 kb on - strand at 2039.452 kb on + strand at 2039.452 kb on + strand at 2039.453 kb on - strand at 2039.454 kb on + strand at 2039.485 kb on + strand at 2039.507 kb on + strand at 2039.507 kb on + strand at 2039.510 kb on + strand at 2039.568 kb on + strand at 2039.616 kb on + strand, within BT1650 at 2039.616 kb on + strand, within BT1650 at 2039.618 kb on + strand, within BT1650 at 2039.618 kb on + strand, within BT1650 at 2039.618 kb on + strand, within BT1650 at 2039.622 kb on + strand, within BT1650 at 2039.637 kb on + strand, within BT1650 at 2039.730 kb on - strand, within BT1650 at 2039.941 kb on - strand, within BT1650 at 2039.943 kb on + strand, within BT1650 at 2039.975 kb on + strand, within BT1650 at 2039.985 kb on + strand, within BT1650 at 2039.985 kb on + strand, within BT1650 at 2039.985 kb on + strand, within BT1650 at 2039.989 kb on + strand, within BT1650 at 2039.989 kb on + strand, within BT1650 at 2040.015 kb on + strand, within BT1650 at 2040.035 kb on + strand, within BT1650 at 2040.113 kb on + strand, within BT1650 at 2040.162 kb on + strand, within BT1650 at 2040.290 kb on + strand, within BT1650 at 2040.290 kb on + strand, within BT1650 at 2040.315 kb on + strand, within BT1650 at 2040.335 kb on + strand, within BT1650
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.0125 mM remove 2,037,740 + -2.4 2,037,740 + -1.4 2,037,853 + BT1648 0.14 -0.9 2,037,900 + BT1648 0.19 -1.0 2,038,036 + BT1648 0.33 -0.2 2,038,040 + BT1648 0.33 -1.7 2,038,040 + BT1648 0.33 +0.1 2,038,040 + BT1648 0.33 -1.1 2,038,040 + BT1648 0.33 +0.3 2,038,042 + BT1648 0.33 +1.1 2,038,066 + BT1648 0.36 +0.1 2,038,067 - BT1648 0.36 +0.1 2,038,095 + BT1648 0.39 +0.1 2,038,116 + BT1648 0.41 -0.9 2,038,128 - BT1648 0.42 +0.1 2,038,130 + BT1648 0.42 -0.6 2,038,131 - BT1648 0.42 -2.7 2,038,135 - BT1648 0.43 +1.1 2,038,154 + BT1648 0.45 +0.1 2,038,170 + BT1648 0.46 -1.5 2,038,227 + BT1648 0.52 -0.1 2,038,228 - BT1648 0.52 -2.1 2,038,279 - BT1648 0.57 -1.5 2,038,291 - BT1648 0.58 +1.1 2,038,332 + BT1648 0.63 +2.7 2,038,362 + BT1648 0.66 +0.1 2,038,383 - BT1648 0.68 +1.1 2,038,406 + BT1648 0.70 -0.7 2,038,414 + BT1648 0.71 -1.7 2,038,416 + BT1648 0.71 -0.4 2,038,421 + BT1648 0.72 +0.5 2,038,421 + BT1648 0.72 -1.7 2,038,423 + BT1648 0.72 -0.4 2,038,533 + BT1648 0.83 +1.1 2,038,626 + -1.4 2,038,626 + +1.1 2,038,627 - +0.1 2,038,640 + -0.7 2,038,643 - +2.1 2,038,656 - +0.3 2,038,755 + -1.9 2,038,776 + -0.5 2,038,832 + BT1649 0.18 -1.7 2,038,832 + BT1649 0.18 -1.3 2,038,981 + BT1649 0.40 +1.1 2,038,981 + BT1649 0.40 +0.1 2,038,982 - BT1649 0.40 -0.9 2,039,005 + BT1649 0.44 +0.1 2,039,005 + BT1649 0.44 -1.5 2,039,023 + BT1649 0.46 -2.5 2,039,034 - BT1649 0.48 -0.3 2,039,053 + BT1649 0.51 -1.5 2,039,076 + BT1649 0.54 -2.4 2,039,077 - BT1649 0.54 -0.5 2,039,104 - BT1649 0.58 -1.8 2,039,135 + BT1649 0.63 +0.1 2,039,135 + BT1649 0.63 -1.6 2,039,177 - BT1649 0.69 -1.5 2,039,222 + BT1649 0.76 -1.5 2,039,222 + BT1649 0.76 +1.1 2,039,324 + +0.9 2,039,390 - -1.9 2,039,452 + -0.5 2,039,452 + -2.2 2,039,453 - -1.3 2,039,454 + +0.8 2,039,485 + -1.3 2,039,507 + -0.3 2,039,507 + +0.1 2,039,510 + -0.3 2,039,568 + -0.5 2,039,616 + BT1650 0.11 -1.1 2,039,616 + BT1650 0.11 +2.1 2,039,618 + BT1650 0.12 -1.1 2,039,618 + BT1650 0.12 +1.1 2,039,618 + BT1650 0.12 +1.1 2,039,622 + BT1650 0.12 +0.7 2,039,637 + BT1650 0.13 -1.2 2,039,730 - BT1650 0.21 -0.9 2,039,941 - BT1650 0.39 -0.9 2,039,943 + BT1650 0.40 -1.7 2,039,975 + BT1650 0.42 -1.4 2,039,985 + BT1650 0.43 +0.5 2,039,985 + BT1650 0.43 -0.3 2,039,985 + BT1650 0.43 +0.5 2,039,989 + BT1650 0.44 -1.0 2,039,989 + BT1650 0.44 -2.1 2,040,015 + BT1650 0.46 -1.5 2,040,035 + BT1650 0.48 -0.8 2,040,113 + BT1650 0.54 -0.7 2,040,162 + BT1650 0.59 +0.7 2,040,290 + BT1650 0.70 -1.3 2,040,290 + BT1650 0.70 -0.9 2,040,315 + BT1650 0.72 -0.6 2,040,335 + BT1650 0.73 +1.1
Or see this region's nucleotide sequence