Experiment: Varel_Bryant medium with Chlorpromazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1512 and BT1513 are separated by 508 nucleotides BT1513 and BT1514 are separated by 397 nucleotides
BT1512: BT1512 - conserved hypothetical protein, putative surface protein (NCBI ptt file), at 1,865,735 to 1,869,355
BT1512
BT1513: BT1513 - integrase (NCBI ptt file), at 1,869,864 to 1,870,781
BT1513
BT1514: BT1514 - hypothetical protein (NCBI ptt file), at 1,871,179 to 1,871,478
BT1514
Position (kb)
1869
1870
1871 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1868.867 kb on - strand, within BT1512 at 1869.008 kb on - strand at 1869.008 kb on - strand at 1869.008 kb on - strand at 1869.125 kb on - strand at 1869.131 kb on + strand at 1869.220 kb on + strand at 1869.221 kb on - strand at 1869.280 kb on + strand at 1869.289 kb on + strand at 1869.434 kb on + strand at 1869.434 kb on + strand at 1869.538 kb on + strand at 1869.543 kb on + strand at 1869.584 kb on + strand at 1869.584 kb on + strand at 1869.584 kb on + strand at 1869.601 kb on + strand at 1869.602 kb on - strand at 1869.606 kb on - strand at 1869.989 kb on + strand, within BT1513 at 1870.027 kb on + strand, within BT1513 at 1870.059 kb on - strand, within BT1513 at 1870.061 kb on - strand, within BT1513 at 1870.099 kb on - strand, within BT1513 at 1870.191 kb on + strand, within BT1513 at 1870.205 kb on + strand, within BT1513 at 1870.223 kb on - strand, within BT1513 at 1870.233 kb on + strand, within BT1513 at 1870.325 kb on + strand, within BT1513 at 1870.502 kb on - strand, within BT1513 at 1870.502 kb on - strand, within BT1513 at 1870.504 kb on - strand, within BT1513 at 1870.812 kb on + strand at 1870.824 kb on + strand at 1870.848 kb on + strand at 1870.879 kb on + strand at 1870.880 kb on - strand at 1871.250 kb on + strand, within BT1514 at 1871.312 kb on + strand, within BT1514 at 1871.519 kb on + strand at 1871.773 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.0125 mM remove 1,868,867 - BT1512 0.86 -2.7 1,869,008 - +0.7 1,869,008 - +0.6 1,869,008 - +1.0 1,869,125 - -0.6 1,869,131 + -1.8 1,869,220 + -1.6 1,869,221 - +0.4 1,869,280 + -0.6 1,869,289 + -2.9 1,869,434 + +1.8 1,869,434 + +0.0 1,869,538 + -0.6 1,869,543 + +1.0 1,869,584 + +0.6 1,869,584 + +1.6 1,869,584 + -0.6 1,869,601 + -1.0 1,869,602 - +0.3 1,869,606 - +0.2 1,869,989 + BT1513 0.14 +1.0 1,870,027 + BT1513 0.18 -0.7 1,870,059 - BT1513 0.21 +1.6 1,870,061 - BT1513 0.21 +0.0 1,870,099 - BT1513 0.26 +1.2 1,870,191 + BT1513 0.36 -0.3 1,870,205 + BT1513 0.37 -3.1 1,870,223 - BT1513 0.39 -0.3 1,870,233 + BT1513 0.40 -0.3 1,870,325 + BT1513 0.50 -2.3 1,870,502 - BT1513 0.69 +0.5 1,870,502 - BT1513 0.69 -0.7 1,870,504 - BT1513 0.70 +1.0 1,870,812 + -0.3 1,870,824 + -1.9 1,870,848 + -1.1 1,870,879 + +0.4 1,870,880 - -0.8 1,871,250 + BT1514 0.24 +0.0 1,871,312 + BT1514 0.44 +0.0 1,871,519 + +0.0 1,871,773 - -2.1
Or see this region's nucleotide sequence