Experiment: Varel_Bryant medium with Chlorpromazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1443 and BT1444 overlap by 4 nucleotides BT1444 and BT1445 are separated by 10 nucleotides BT1445 and BT1446 overlap by 4 nucleotides BT1446 and BT1447 are separated by 24 nucleotides
BT1443: BT1443 - 8-amino-7-oxononanoate synthase (NCBI ptt file), at 1,777,882 to 1,779,036
BT1443
BT1444: BT1444 - conserved hypothetical protein (NCBI ptt file), at 1,779,033 to 1,779,692
BT1444
BT1445: BT1445 - putative biotin synthesis protein bioC (NCBI ptt file), at 1,779,703 to 1,780,488
BT1445
BT1446: BT1446 - dethiobiotin synthetase (NCBI ptt file), at 1,780,485 to 1,781,132
BT1446
BT1447: BT1447 - two-component system sensor histidine kinase (NCBI ptt file), at 1,781,157 to 1,783,514
BT1447
Position (kb)
1779
1780
1781 Strain fitness (log2 ratio)
-1
0
1
2 at 1778.800 kb on + strand, within BT1443 at 1778.821 kb on + strand, within BT1443 at 1778.824 kb on - strand, within BT1443 at 1778.824 kb on - strand, within BT1443 at 1778.824 kb on - strand, within BT1443 at 1778.917 kb on + strand, within BT1443 at 1778.918 kb on - strand, within BT1443 at 1779.017 kb on + strand at 1779.122 kb on + strand, within BT1444 at 1779.190 kb on + strand, within BT1444 at 1779.392 kb on - strand, within BT1444 at 1779.451 kb on + strand, within BT1444 at 1779.686 kb on + strand at 1779.708 kb on + strand at 1779.708 kb on + strand at 1779.923 kb on + strand, within BT1445 at 1779.941 kb on - strand, within BT1445 at 1779.956 kb on + strand, within BT1445 at 1780.024 kb on - strand, within BT1445 at 1780.032 kb on + strand, within BT1445 at 1780.033 kb on - strand, within BT1445 at 1780.033 kb on - strand, within BT1445 at 1780.092 kb on + strand, within BT1445 at 1780.093 kb on - strand, within BT1445 at 1780.187 kb on - strand, within BT1445 at 1780.234 kb on - strand, within BT1445 at 1780.234 kb on - strand, within BT1445 at 1780.235 kb on + strand, within BT1445 at 1780.447 kb on + strand at 1780.448 kb on - strand at 1780.448 kb on - strand at 1780.448 kb on - strand at 1780.460 kb on - strand at 1780.460 kb on - strand at 1780.467 kb on - strand at 1780.467 kb on - strand at 1780.485 kb on - strand at 1780.639 kb on + strand, within BT1446 at 1780.639 kb on + strand, within BT1446 at 1780.640 kb on - strand, within BT1446 at 1780.640 kb on - strand, within BT1446 at 1780.791 kb on - strand, within BT1446 at 1780.884 kb on + strand, within BT1446 at 1780.885 kb on - strand, within BT1446 at 1780.933 kb on + strand, within BT1446 at 1780.933 kb on + strand, within BT1446 at 1780.934 kb on - strand, within BT1446 at 1780.997 kb on + strand, within BT1446 at 1780.997 kb on + strand, within BT1446 at 1781.003 kb on - strand, within BT1446 at 1781.009 kb on + strand, within BT1446 at 1781.034 kb on - strand, within BT1446 at 1781.051 kb on + strand, within BT1446 at 1781.064 kb on - strand, within BT1446 at 1781.064 kb on - strand, within BT1446 at 1781.139 kb on + strand at 1781.139 kb on + strand at 1781.140 kb on - strand at 1781.141 kb on + strand at 1781.142 kb on - strand at 1781.156 kb on - strand at 1781.454 kb on - strand, within BT1447 at 1781.467 kb on - strand, within BT1447
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.0125 mM remove 1,778,800 + BT1443 0.79 +0.7 1,778,821 + BT1443 0.81 -0.1 1,778,824 - BT1443 0.82 +2.1 1,778,824 - BT1443 0.82 -0.3 1,778,824 - BT1443 0.82 +1.1 1,778,917 + BT1443 0.90 +0.6 1,778,918 - BT1443 0.90 +1.1 1,779,017 + +0.1 1,779,122 + BT1444 0.13 +0.5 1,779,190 + BT1444 0.24 +2.7 1,779,392 - BT1444 0.54 -0.7 1,779,451 + BT1444 0.63 +1.1 1,779,686 + +1.1 1,779,708 + -0.5 1,779,708 + +0.6 1,779,923 + BT1445 0.28 -1.1 1,779,941 - BT1445 0.30 +1.3 1,779,956 + BT1445 0.32 -0.0 1,780,024 - BT1445 0.41 +2.1 1,780,032 + BT1445 0.42 +0.1 1,780,033 - BT1445 0.42 +1.6 1,780,033 - BT1445 0.42 -0.5 1,780,092 + BT1445 0.49 +0.8 1,780,093 - BT1445 0.50 -0.3 1,780,187 - BT1445 0.62 +0.1 1,780,234 - BT1445 0.68 +1.7 1,780,234 - BT1445 0.68 -0.2 1,780,235 + BT1445 0.68 -0.4 1,780,447 + +0.0 1,780,448 - +1.4 1,780,448 - -0.7 1,780,448 - -0.4 1,780,460 - +0.7 1,780,460 - +1.3 1,780,467 - +0.5 1,780,467 - -0.3 1,780,485 - -1.7 1,780,639 + BT1446 0.24 +1.8 1,780,639 + BT1446 0.24 +0.2 1,780,640 - BT1446 0.24 +0.9 1,780,640 - BT1446 0.24 +1.8 1,780,791 - BT1446 0.47 +1.0 1,780,884 + BT1446 0.62 -0.1 1,780,885 - BT1446 0.62 +0.3 1,780,933 + BT1446 0.69 +0.7 1,780,933 + BT1446 0.69 +0.1 1,780,934 - BT1446 0.69 -0.7 1,780,997 + BT1446 0.79 +1.1 1,780,997 + BT1446 0.79 -0.7 1,781,003 - BT1446 0.80 +2.1 1,781,009 + BT1446 0.81 +0.4 1,781,034 - BT1446 0.85 +1.0 1,781,051 + BT1446 0.87 -0.5 1,781,064 - BT1446 0.89 -0.1 1,781,064 - BT1446 0.89 +1.1 1,781,139 + +1.1 1,781,139 + -0.9 1,781,140 - -1.3 1,781,141 + +0.5 1,781,142 - +0.5 1,781,156 - -0.7 1,781,454 - BT1447 0.13 -0.3 1,781,467 - BT1447 0.13 -1.3
Or see this region's nucleotide sequence