Experiment: Varel_Bryant medium with Chlorpromazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0530 and BT0531 are separated by 4 nucleotides BT0531 and BT0532 are separated by 68 nucleotides BT0532 and BT0533 are separated by 44 nucleotides
BT0530: BT0530 - anthranilate phosphoribosyltransferase (EC 2.4.2.18) <small>(from data)</small>, at 653,313 to 654,311
BT0530
BT0531: BT0531 - anthranilate synthase component II (NCBI ptt file), at 654,316 to 654,882
BT0531
BT0532: BT0532 - anthranilate synthase component I (NCBI ptt file), at 654,951 to 656,357
BT0532
BT0533: BT0533 - tryptophan synthase beta chain (NCBI ptt file), at 656,402 to 657,586
BT0533
Position (kb) 654
655
656
657 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 653.973 kb on - strand, within BT0530 at 653.995 kb on - strand, within BT0530 at 654.154 kb on - strand, within BT0530 at 654.191 kb on + strand, within BT0530 at 654.248 kb on + strand at 654.250 kb on + strand at 654.251 kb on + strand at 654.275 kb on - strand at 654.293 kb on + strand at 654.296 kb on - strand at 654.416 kb on - strand, within BT0531 at 654.451 kb on + strand, within BT0531 at 654.541 kb on + strand, within BT0531 at 654.575 kb on - strand, within BT0531 at 654.688 kb on - strand, within BT0531 at 654.739 kb on + strand, within BT0531 at 654.999 kb on + strand at 655.000 kb on - strand at 655.011 kb on + strand at 655.250 kb on + strand at 655.333 kb on + strand, within BT0532 at 655.346 kb on + strand, within BT0532 at 655.363 kb on - strand, within BT0532 at 655.363 kb on - strand, within BT0532 at 655.797 kb on - strand, within BT0532 at 655.798 kb on + strand, within BT0532 at 655.917 kb on - strand, within BT0532 at 655.922 kb on + strand, within BT0532 at 655.922 kb on + strand, within BT0532 at 655.922 kb on + strand, within BT0532 at 655.922 kb on + strand, within BT0532 at 655.985 kb on + strand, within BT0532 at 656.151 kb on - strand, within BT0532 at 656.160 kb on + strand, within BT0532 at 656.160 kb on + strand, within BT0532 at 656.267 kb on - strand at 656.292 kb on - strand at 656.292 kb on - strand at 656.309 kb on + strand at 656.310 kb on - strand at 656.320 kb on - strand at 656.340 kb on - strand at 656.397 kb on + strand at 656.566 kb on - strand, within BT0533 at 656.762 kb on + strand, within BT0533 at 656.762 kb on + strand, within BT0533 at 656.842 kb on + strand, within BT0533 at 656.850 kb on + strand, within BT0533 at 657.024 kb on + strand, within BT0533 at 657.187 kb on + strand, within BT0533 at 657.212 kb on + strand, within BT0533 at 657.212 kb on + strand, within BT0533 at 657.213 kb on - strand, within BT0533 at 657.331 kb on + strand, within BT0533
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.0125 mM remove 653,973 - BT0530 0.66 -2.7 653,995 - BT0530 0.68 -0.0 654,154 - BT0530 0.84 -0.0 654,191 + BT0530 0.88 -1.2 654,248 + -3.6 654,250 + -0.0 654,251 + -2.0 654,275 - -0.0 654,293 + -3.4 654,296 - -4.1 654,416 - BT0531 0.18 -0.2 654,451 + BT0531 0.24 -2.2 654,541 + BT0531 0.40 -2.8 654,575 - BT0531 0.46 -2.7 654,688 - BT0531 0.66 -2.0 654,739 + BT0531 0.75 -1.3 654,999 + -1.3 655,000 - -0.6 655,011 + -2.8 655,250 + -0.6 655,333 + BT0532 0.27 -4.1 655,346 + BT0532 0.28 -0.9 655,363 - BT0532 0.29 -1.0 655,363 - BT0532 0.29 +1.0 655,797 - BT0532 0.60 -4.7 655,798 + BT0532 0.60 -2.5 655,917 - BT0532 0.69 -2.8 655,922 + BT0532 0.69 -3.6 655,922 + BT0532 0.69 -2.1 655,922 + BT0532 0.69 -1.3 655,922 + BT0532 0.69 -2.7 655,985 + BT0532 0.73 -2.0 656,151 - BT0532 0.85 -1.3 656,160 + BT0532 0.86 -2.2 656,160 + BT0532 0.86 +1.0 656,267 - -1.8 656,292 - -0.6 656,292 - +1.0 656,309 + -4.3 656,310 - -1.3 656,320 - -0.0 656,340 - -2.9 656,397 + -2.0 656,566 - BT0533 0.14 +1.0 656,762 + BT0533 0.30 -3.7 656,762 + BT0533 0.30 -0.6 656,842 + BT0533 0.37 -1.3 656,850 + BT0533 0.38 +1.0 657,024 + BT0533 0.52 -0.0 657,187 + BT0533 0.66 -1.3 657,212 + BT0533 0.68 -2.3 657,212 + BT0533 0.68 -2.0 657,213 - BT0533 0.68 +1.0 657,331 + BT0533 0.78 +1.0
Or see this region's nucleotide sequence