Experiment: Varel_Bryant medium with Chlorpromazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0406 and BT0407 are separated by 8 nucleotides BT0407 and BT0408 are separated by 28 nucleotides BT0408 and BT0409 are separated by 201 nucleotides BT0409 and BT0410 are separated by 117 nucleotides BT0410 and BT0411 are separated by 211 nucleotides
BT0406: BT0406 - hypothetical protein (NCBI ptt file), at 494,471 to 494,728
BT0406
BT0407: BT0407 - hypothetical protein (NCBI ptt file), at 494,737 to 495,096
BT0407
BT0408: BT0408 - hypothetical protein (NCBI ptt file), at 495,125 to 495,334
BT0408
BT0409: BT0409 - putative short-chain dehydrogenase (NCBI ptt file), at 495,536 to 496,306
BT0409
BT0410: BT0410 - hypothetical protein (NCBI ptt file), at 496,424 to 496,918
BT0410
BT0411: BT0411 - CysQ, sulfite synthesis pathway protein (NCBI ptt file), at 497,130 to 497,936
BT0411
Position (kb) 495
496
497 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 494.696 kb on + strand, within BT0406 at 494.729 kb on - strand at 494.739 kb on - strand at 494.826 kb on + strand, within BT0407 at 494.981 kb on - strand, within BT0407 at 495.242 kb on - strand, within BT0408 at 495.318 kb on - strand at 495.339 kb on + strand at 495.464 kb on - strand at 495.641 kb on - strand, within BT0409 at 495.693 kb on + strand, within BT0409 at 495.786 kb on + strand, within BT0409 at 495.875 kb on - strand, within BT0409 at 495.876 kb on + strand, within BT0409 at 495.877 kb on - strand, within BT0409 at 495.877 kb on - strand, within BT0409 at 495.995 kb on + strand, within BT0409 at 496.205 kb on + strand, within BT0409 at 496.286 kb on + strand at 496.288 kb on + strand at 496.305 kb on - strand at 496.606 kb on + strand, within BT0410 at 496.612 kb on + strand, within BT0410 at 496.686 kb on + strand, within BT0410 at 496.865 kb on + strand, within BT0410 at 496.909 kb on + strand at 496.913 kb on + strand at 496.991 kb on - strand at 497.128 kb on + strand at 497.178 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.0125 mM remove 494,696 + BT0406 0.87 -1.6 494,729 - -2.9 494,739 - +0.9 494,826 + BT0407 0.25 -1.1 494,981 - BT0407 0.68 +0.4 495,242 - BT0408 0.56 -1.1 495,318 - -0.1 495,339 + -1.6 495,464 - -0.9 495,641 - BT0409 0.14 -0.1 495,693 + BT0409 0.20 -2.4 495,786 + BT0409 0.32 -3.3 495,875 - BT0409 0.44 +0.9 495,876 + BT0409 0.44 -1.4 495,877 - BT0409 0.44 -0.1 495,877 - BT0409 0.44 -2.1 495,995 + BT0409 0.60 -1.4 496,205 + BT0409 0.87 -1.1 496,286 + +1.5 496,288 + -0.1 496,305 - +1.9 496,606 + BT0410 0.37 -0.6 496,612 + BT0410 0.38 -2.1 496,686 + BT0410 0.53 -2.8 496,865 + BT0410 0.89 -2.1 496,909 + -2.0 496,913 + +0.9 496,991 - -1.5 497,128 + -0.3 497,178 - +0.9
Or see this region's nucleotide sequence