Experiment: Spectinomycin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_849 and Pf6N2E2_850 are separated by 282 nucleotides Pf6N2E2_850 and Pf6N2E2_851 are separated by 96 nucleotides Pf6N2E2_851 and Pf6N2E2_852 are separated by 227 nucleotides
Pf6N2E2_849 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 993,711 to 995,078
_849
Pf6N2E2_850 - hypothetical protein, at 995,361 to 995,474
_850
Pf6N2E2_851 - Osmotically inducible protein Y precursor, at 995,571 to 996,218
_851
Pf6N2E2_852 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 996,446 to 998,728
_852
Position (kb) 995
996
997 Strain fitness (log2 ratio) -1
0
1
2
3 at 994.607 kb on - strand, within Pf6N2E2_849 at 994.771 kb on + strand, within Pf6N2E2_849 at 994.771 kb on + strand, within Pf6N2E2_849 at 994.772 kb on - strand, within Pf6N2E2_849 at 994.772 kb on - strand, within Pf6N2E2_849 at 994.870 kb on - strand, within Pf6N2E2_849 at 994.873 kb on + strand, within Pf6N2E2_849 at 994.873 kb on + strand, within Pf6N2E2_849 at 994.874 kb on - strand, within Pf6N2E2_849 at 994.874 kb on - strand, within Pf6N2E2_849 at 995.018 kb on + strand at 995.019 kb on - strand at 995.029 kb on + strand at 995.030 kb on - strand at 995.041 kb on + strand at 995.125 kb on + strand at 995.125 kb on + strand at 995.125 kb on + strand at 995.125 kb on + strand at 995.126 kb on - strand at 995.126 kb on - strand at 995.126 kb on - strand at 995.215 kb on + strand at 995.219 kb on + strand at 995.219 kb on + strand at 995.219 kb on + strand at 995.220 kb on - strand at 995.225 kb on + strand at 995.226 kb on - strand at 995.290 kb on - strand at 995.297 kb on + strand at 995.323 kb on - strand at 995.362 kb on + strand at 995.739 kb on - strand, within Pf6N2E2_851 at 995.864 kb on - strand, within Pf6N2E2_851 at 995.928 kb on + strand, within Pf6N2E2_851 at 995.929 kb on - strand, within Pf6N2E2_851 at 995.929 kb on - strand, within Pf6N2E2_851 at 996.015 kb on + strand, within Pf6N2E2_851 at 996.015 kb on + strand, within Pf6N2E2_851 at 996.015 kb on + strand, within Pf6N2E2_851 at 996.016 kb on - strand, within Pf6N2E2_851 at 996.016 kb on - strand, within Pf6N2E2_851 at 996.016 kb on - strand at 996.192 kb on - strand at 996.197 kb on + strand at 996.267 kb on + strand at 996.305 kb on + strand at 996.306 kb on - strand at 996.340 kb on + strand at 996.431 kb on + strand at 996.446 kb on - strand at 996.699 kb on + strand, within Pf6N2E2_852 at 996.867 kb on + strand, within Pf6N2E2_852 at 996.868 kb on - strand, within Pf6N2E2_852 at 997.184 kb on + strand, within Pf6N2E2_852 at 997.185 kb on - strand, within Pf6N2E2_852 at 997.185 kb on - strand, within Pf6N2E2_852 at 997.185 kb on - strand, within Pf6N2E2_852 at 997.185 kb on - strand, within Pf6N2E2_852 at 997.185 kb on - strand, within Pf6N2E2_852
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.01 mg/ml remove 994,607 - Pf6N2E2_849 0.65 +0.3 994,771 + Pf6N2E2_849 0.77 -0.3 994,771 + Pf6N2E2_849 0.77 +1.1 994,772 - Pf6N2E2_849 0.78 -0.3 994,772 - Pf6N2E2_849 0.78 -0.0 994,870 - Pf6N2E2_849 0.85 -0.3 994,873 + Pf6N2E2_849 0.85 +1.1 994,873 + Pf6N2E2_849 0.85 +0.6 994,874 - Pf6N2E2_849 0.85 +0.7 994,874 - Pf6N2E2_849 0.85 +0.1 995,018 + -1.2 995,019 - -0.0 995,029 + +0.7 995,030 - +0.3 995,041 + +1.6 995,125 + -0.2 995,125 + -0.6 995,125 + +0.1 995,125 + +0.4 995,126 - +3.2 995,126 - +0.4 995,126 - -0.0 995,215 + -0.3 995,219 + +0.2 995,219 + -0.7 995,219 + -0.2 995,220 - -0.6 995,225 + +0.2 995,226 - -0.2 995,290 - +0.6 995,297 + +0.7 995,323 - +0.7 995,362 + +0.4 995,739 - Pf6N2E2_851 0.26 +0.4 995,864 - Pf6N2E2_851 0.45 -0.3 995,928 + Pf6N2E2_851 0.55 +0.7 995,929 - Pf6N2E2_851 0.55 +0.3 995,929 - Pf6N2E2_851 0.55 +1.5 996,015 + Pf6N2E2_851 0.69 -0.0 996,015 + Pf6N2E2_851 0.69 +1.9 996,015 + Pf6N2E2_851 0.69 +1.7 996,016 - Pf6N2E2_851 0.69 +0.0 996,016 - Pf6N2E2_851 0.69 +1.4 996,016 - +0.4 996,192 - +0.4 996,197 + -0.2 996,267 + -0.3 996,305 + +0.2 996,306 - +0.2 996,340 + -0.1 996,431 + +0.1 996,446 - +1.0 996,699 + Pf6N2E2_852 0.11 +0.4 996,867 + Pf6N2E2_852 0.18 +1.3 996,868 - Pf6N2E2_852 0.18 +0.4 997,184 + Pf6N2E2_852 0.32 -0.6 997,185 - Pf6N2E2_852 0.32 +1.0 997,185 - Pf6N2E2_852 0.32 -0.2 997,185 - Pf6N2E2_852 0.32 +0.4 997,185 - Pf6N2E2_852 0.32 -1.2 997,185 - Pf6N2E2_852 0.32 -1.6
Or see this region's nucleotide sequence