Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_846

Experiment: Spectinomycin 0.01 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_844 and Pf6N2E2_845 are separated by 112 nucleotidesPf6N2E2_845 and Pf6N2E2_846 are separated by 311 nucleotidesPf6N2E2_846 and Pf6N2E2_847 are separated by 52 nucleotidesPf6N2E2_847 and Pf6N2E2_848 overlap by 8 nucleotides Pf6N2E2_844 - Ribose-phosphate pyrophosphokinase (EC 2.7.6.1), at 989,411 to 990,403 _844 Pf6N2E2_845 - phosphoribosyltransferase, at 990,516 to 991,082 _845 Pf6N2E2_846 - Universal stress protein family 5, at 991,394 to 992,284 _846 Pf6N2E2_847 - Phosphoribosyl transferase domain protein, at 992,337 to 993,041 _847 Pf6N2E2_848 - hypothetical protein, at 993,034 to 993,729 _848 Position (kb) 991 992 993Strain fitness (log2 ratio) -2 -1 0 1 2at 990.453 kb on + strandat 990.454 kb on - strandat 990.454 kb on - strandat 990.454 kb on - strandat 990.554 kb on - strandat 990.554 kb on - strandat 990.554 kb on - strandat 990.554 kb on - strandat 990.615 kb on + strand, within Pf6N2E2_845at 990.615 kb on + strand, within Pf6N2E2_845at 990.616 kb on - strand, within Pf6N2E2_845at 990.616 kb on - strand, within Pf6N2E2_845at 990.616 kb on - strand, within Pf6N2E2_845at 990.654 kb on - strand, within Pf6N2E2_845at 990.670 kb on - strand, within Pf6N2E2_845at 990.677 kb on + strand, within Pf6N2E2_845at 990.773 kb on + strand, within Pf6N2E2_845at 990.773 kb on + strand, within Pf6N2E2_845at 990.773 kb on + strand, within Pf6N2E2_845at 990.823 kb on + strand, within Pf6N2E2_845at 990.823 kb on + strand, within Pf6N2E2_845at 990.824 kb on - strand, within Pf6N2E2_845at 990.827 kb on + strand, within Pf6N2E2_845at 990.827 kb on + strand, within Pf6N2E2_845at 990.827 kb on + strand, within Pf6N2E2_845at 990.827 kb on + strand, within Pf6N2E2_845at 990.827 kb on + strand, within Pf6N2E2_845at 990.827 kb on + strand, within Pf6N2E2_845at 990.827 kb on + strand, within Pf6N2E2_845at 990.827 kb on + strand, within Pf6N2E2_845at 990.828 kb on - strand, within Pf6N2E2_845at 990.828 kb on - strand, within Pf6N2E2_845at 990.828 kb on - strand, within Pf6N2E2_845at 990.828 kb on - strand, within Pf6N2E2_845at 990.828 kb on - strand, within Pf6N2E2_845at 990.913 kb on + strand, within Pf6N2E2_845at 990.913 kb on + strand, within Pf6N2E2_845at 990.914 kb on - strand, within Pf6N2E2_845at 990.914 kb on - strand, within Pf6N2E2_845at 990.914 kb on - strand, within Pf6N2E2_845at 990.914 kb on - strand, within Pf6N2E2_845at 990.914 kb on - strand, within Pf6N2E2_845at 991.046 kb on - strandat 991.046 kb on - strandat 991.046 kb on - strandat 991.046 kb on - strandat 991.071 kb on + strandat 991.072 kb on - strandat 991.101 kb on - strandat 991.113 kb on + strandat 991.113 kb on + strandat 991.113 kb on + strandat 991.114 kb on - strandat 991.114 kb on - strandat 991.114 kb on - strandat 991.117 kb on + strandat 991.117 kb on + strandat 991.118 kb on - strandat 991.118 kb on - strandat 991.126 kb on + strandat 991.255 kb on - strandat 991.255 kb on - strandat 991.255 kb on - strandat 991.261 kb on + strandat 991.261 kb on + strandat 991.262 kb on - strandat 991.262 kb on - strandat 991.262 kb on - strandat 991.262 kb on - strandat 991.262 kb on - strandat 991.262 kb on - strandat 991.262 kb on - strandat 991.263 kb on + strandat 991.263 kb on + strandat 991.263 kb on + strandat 991.263 kb on + strandat 991.263 kb on + strandat 991.263 kb on + strandat 991.264 kb on - strandat 991.264 kb on - strandat 991.264 kb on - strandat 991.264 kb on - strandat 991.264 kb on - strandat 991.264 kb on - strandat 991.334 kb on + strandat 991.334 kb on + strandat 991.334 kb on + strandat 991.335 kb on - strandat 991.335 kb on - strandat 991.335 kb on - strandat 991.335 kb on - strandat 991.403 kb on + strandat 991.413 kb on + strandat 991.413 kb on + strandat 991.577 kb on + strand, within Pf6N2E2_846at 991.578 kb on - strand, within Pf6N2E2_846at 991.814 kb on + strand, within Pf6N2E2_846at 991.815 kb on - strand, within Pf6N2E2_846at 991.970 kb on + strand, within Pf6N2E2_846at 991.970 kb on + strand, within Pf6N2E2_846at 991.994 kb on + strand, within Pf6N2E2_846at 991.994 kb on + strand, within Pf6N2E2_846at 991.994 kb on + strand, within Pf6N2E2_846at 991.994 kb on + strand, within Pf6N2E2_846at 991.994 kb on + strand, within Pf6N2E2_846at 991.994 kb on + strand, within Pf6N2E2_846at 991.994 kb on + strand, within Pf6N2E2_846at 991.994 kb on + strand, within Pf6N2E2_846at 991.994 kb on + strand, within Pf6N2E2_846at 991.995 kb on - strand, within Pf6N2E2_846at 991.995 kb on - strand, within Pf6N2E2_846at 991.995 kb on - strand, within Pf6N2E2_846at 991.995 kb on - strand, within Pf6N2E2_846at 991.995 kb on - strand, within Pf6N2E2_846at 991.995 kb on - strand, within Pf6N2E2_846at 992.173 kb on + strand, within Pf6N2E2_846at 992.173 kb on + strand, within Pf6N2E2_846at 992.173 kb on + strand, within Pf6N2E2_846at 992.173 kb on + strand, within Pf6N2E2_846at 992.174 kb on - strand, within Pf6N2E2_846at 992.174 kb on - strand, within Pf6N2E2_846at 992.174 kb on - strand, within Pf6N2E2_846at 992.231 kb on - strandat 992.245 kb on + strandat 992.245 kb on + strandat 992.246 kb on - strandat 992.259 kb on + strandat 992.259 kb on + strandat 992.282 kb on + strandat 992.282 kb on + strandat 992.282 kb on + strandat 992.282 kb on + strandat 992.587 kb on + strand, within Pf6N2E2_847at 992.588 kb on - strand, within Pf6N2E2_847at 992.798 kb on - strand, within Pf6N2E2_847at 992.934 kb on + strand, within Pf6N2E2_847at 992.934 kb on + strand, within Pf6N2E2_847at 992.935 kb on - strand, within Pf6N2E2_847at 993.172 kb on + strand, within Pf6N2E2_848at 993.172 kb on + strand, within Pf6N2E2_848at 993.173 kb on - strand, within Pf6N2E2_848at 993.173 kb on - strand, within Pf6N2E2_848

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.01 mg/ml
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990,453 + -0.7
990,454 - +0.4
990,454 - +1.0
990,454 - +0.9
990,554 - +0.2
990,554 - +0.3
990,554 - -0.6
990,554 - -0.1
990,615 + Pf6N2E2_845 0.17 +0.9
990,615 + Pf6N2E2_845 0.17 +0.1
990,616 - Pf6N2E2_845 0.18 +0.6
990,616 - Pf6N2E2_845 0.18 -0.1
990,616 - Pf6N2E2_845 0.18 -0.1
990,654 - Pf6N2E2_845 0.24 -0.4
990,670 - Pf6N2E2_845 0.27 +0.3
990,677 + Pf6N2E2_845 0.28 +1.9
990,773 + Pf6N2E2_845 0.45 -0.1
990,773 + Pf6N2E2_845 0.45 -0.8
990,773 + Pf6N2E2_845 0.45 +0.0
990,823 + Pf6N2E2_845 0.54 -0.2
990,823 + Pf6N2E2_845 0.54 -1.1
990,824 - Pf6N2E2_845 0.54 -0.1
990,827 + Pf6N2E2_845 0.55 -0.0
990,827 + Pf6N2E2_845 0.55 -0.2
990,827 + Pf6N2E2_845 0.55 -1.0
990,827 + Pf6N2E2_845 0.55 +0.3
990,827 + Pf6N2E2_845 0.55 -0.6
990,827 + Pf6N2E2_845 0.55 +0.2
990,827 + Pf6N2E2_845 0.55 +0.4
990,827 + Pf6N2E2_845 0.55 +1.0
990,828 - Pf6N2E2_845 0.55 +0.6
990,828 - Pf6N2E2_845 0.55 +0.3
990,828 - Pf6N2E2_845 0.55 +0.8
990,828 - Pf6N2E2_845 0.55 +0.1
990,828 - Pf6N2E2_845 0.55 +0.1
990,913 + Pf6N2E2_845 0.70 -0.8
990,913 + Pf6N2E2_845 0.70 +1.2
990,914 - Pf6N2E2_845 0.70 +0.1
990,914 - Pf6N2E2_845 0.70 -0.8
990,914 - Pf6N2E2_845 0.70 -0.7
990,914 - Pf6N2E2_845 0.70 +0.4
990,914 - Pf6N2E2_845 0.70 -0.9
991,046 - -1.1
991,046 - -0.3
991,046 - -1.3
991,046 - -1.3
991,071 + +1.1
991,072 - -0.2
991,101 - +0.1
991,113 + -0.7
991,113 + -0.6
991,113 + +0.8
991,114 - +0.1
991,114 - -0.7
991,114 - -1.8
991,117 + +0.9
991,117 + +1.4
991,118 - -1.4
991,118 - -0.6
991,126 + -0.7
991,255 - +0.5
991,255 - -0.0
991,255 - -1.1
991,261 + -0.3
991,261 + -0.1
991,262 - +0.9
991,262 - -2.4
991,262 - +0.3
991,262 - -1.1
991,262 - +0.7
991,262 - +1.9
991,262 - +2.3
991,263 + -0.8
991,263 + -0.1
991,263 + -2.2
991,263 + -1.9
991,263 + +1.9
991,263 + -1.9
991,264 - +0.6
991,264 - -0.0
991,264 - -0.8
991,264 - -0.6
991,264 - +0.0
991,264 - +0.7
991,334 + -0.1
991,334 + -0.1
991,334 + -1.1
991,335 - +0.4
991,335 - +0.2
991,335 - -0.7
991,335 - +1.4
991,403 + +0.3
991,413 + +0.3
991,413 + -0.8
991,577 + Pf6N2E2_846 0.21 -0.0
991,578 - Pf6N2E2_846 0.21 +1.0
991,814 + Pf6N2E2_846 0.47 +1.5
991,815 - Pf6N2E2_846 0.47 +0.0
991,970 + Pf6N2E2_846 0.65 +0.3
991,970 + Pf6N2E2_846 0.65 +0.4
991,994 + Pf6N2E2_846 0.67 -1.9
991,994 + Pf6N2E2_846 0.67 +2.3
991,994 + Pf6N2E2_846 0.67 +0.3
991,994 + Pf6N2E2_846 0.67 +0.4
991,994 + Pf6N2E2_846 0.67 +1.0
991,994 + Pf6N2E2_846 0.67 +1.1
991,994 + Pf6N2E2_846 0.67 +1.3
991,994 + Pf6N2E2_846 0.67 -0.0
991,994 + Pf6N2E2_846 0.67 +0.3
991,995 - Pf6N2E2_846 0.67 -0.4
991,995 - Pf6N2E2_846 0.67 +0.4
991,995 - Pf6N2E2_846 0.67 +0.7
991,995 - Pf6N2E2_846 0.67 -1.9
991,995 - Pf6N2E2_846 0.67 -0.9
991,995 - Pf6N2E2_846 0.67 +1.0
992,173 + Pf6N2E2_846 0.87 +1.1
992,173 + Pf6N2E2_846 0.87 +0.9
992,173 + Pf6N2E2_846 0.87 +1.7
992,173 + Pf6N2E2_846 0.87 -0.1
992,174 - Pf6N2E2_846 0.88 -1.1
992,174 - Pf6N2E2_846 0.88 +0.2
992,174 - Pf6N2E2_846 0.88 +0.8
992,231 - +0.4
992,245 + -0.4
992,245 + -0.6
992,246 - +0.8
992,259 + +1.4
992,259 + +2.0
992,282 + -0.3
992,282 + +0.4
992,282 + -0.1
992,282 + -0.4
992,587 + Pf6N2E2_847 0.35 -0.1
992,588 - Pf6N2E2_847 0.36 -0.6
992,798 - Pf6N2E2_847 0.65 +0.9
992,934 + Pf6N2E2_847 0.85 -0.6
992,934 + Pf6N2E2_847 0.85 +0.2
992,935 - Pf6N2E2_847 0.85 +0.4
993,172 + Pf6N2E2_848 0.20 -1.1
993,172 + Pf6N2E2_848 0.20 -0.6
993,173 - Pf6N2E2_848 0.20 -0.1
993,173 - Pf6N2E2_848 0.20 -0.0

Or see this region's nucleotide sequence