Experiment: Spectinomycin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_619 and Pf6N2E2_620 are separated by 337 nucleotides Pf6N2E2_620 and Pf6N2E2_621 overlap by 4 nucleotides
Pf6N2E2_619 - Exonuclease SbcD, at 704,047 to 705,291
_619
Pf6N2E2_620 - BatD, at 705,629 to 707,266
_620
Pf6N2E2_621 - TPR domain protein in aerotolerance operon, at 707,263 to 708,999
_621
Position (kb) 705
706
707
708 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 704.770 kb on - strand, within Pf6N2E2_619 at 705.102 kb on - strand, within Pf6N2E2_619 at 705.147 kb on + strand, within Pf6N2E2_619 at 705.296 kb on + strand at 705.296 kb on + strand at 705.579 kb on + strand at 705.630 kb on + strand at 705.631 kb on - strand at 705.754 kb on - strand at 705.892 kb on + strand, within Pf6N2E2_620 at 705.893 kb on - strand, within Pf6N2E2_620 at 705.893 kb on - strand, within Pf6N2E2_620 at 706.036 kb on - strand, within Pf6N2E2_620 at 706.488 kb on + strand, within Pf6N2E2_620 at 706.653 kb on + strand, within Pf6N2E2_620 at 706.653 kb on + strand, within Pf6N2E2_620 at 706.654 kb on - strand, within Pf6N2E2_620 at 706.654 kb on - strand, within Pf6N2E2_620 at 706.710 kb on + strand, within Pf6N2E2_620 at 706.782 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.836 kb on + strand, within Pf6N2E2_620 at 706.837 kb on - strand, within Pf6N2E2_620 at 706.837 kb on - strand, within Pf6N2E2_620 at 706.837 kb on - strand, within Pf6N2E2_620 at 706.837 kb on - strand, within Pf6N2E2_620 at 707.247 kb on + strand at 707.247 kb on + strand at 707.248 kb on - strand at 707.248 kb on - strand at 707.313 kb on + strand at 707.458 kb on + strand, within Pf6N2E2_621 at 707.458 kb on + strand, within Pf6N2E2_621 at 707.458 kb on + strand, within Pf6N2E2_621 at 707.459 kb on - strand, within Pf6N2E2_621 at 707.723 kb on + strand, within Pf6N2E2_621 at 707.723 kb on + strand, within Pf6N2E2_621 at 707.723 kb on + strand, within Pf6N2E2_621 at 707.723 kb on + strand, within Pf6N2E2_621 at 707.780 kb on + strand, within Pf6N2E2_621 at 707.780 kb on + strand, within Pf6N2E2_621 at 707.781 kb on - strand, within Pf6N2E2_621 at 707.995 kb on + strand, within Pf6N2E2_621 at 707.995 kb on + strand, within Pf6N2E2_621 at 707.996 kb on - strand, within Pf6N2E2_621
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.01 mg/ml remove 704,770 - Pf6N2E2_619 0.58 +0.3 705,102 - Pf6N2E2_619 0.85 -0.6 705,147 + Pf6N2E2_619 0.88 -0.1 705,296 + +0.2 705,296 + -0.2 705,579 + -0.5 705,630 + -0.8 705,631 - -1.3 705,754 - -3.2 705,892 + Pf6N2E2_620 0.16 -1.8 705,893 - Pf6N2E2_620 0.16 -2.8 705,893 - Pf6N2E2_620 0.16 -2.1 706,036 - Pf6N2E2_620 0.25 -2.0 706,488 + Pf6N2E2_620 0.52 -2.1 706,653 + Pf6N2E2_620 0.63 -2.6 706,653 + Pf6N2E2_620 0.63 -3.3 706,654 - Pf6N2E2_620 0.63 -2.5 706,654 - Pf6N2E2_620 0.63 -2.2 706,710 + Pf6N2E2_620 0.66 -2.1 706,782 + Pf6N2E2_620 0.70 -4.2 706,836 + Pf6N2E2_620 0.74 -4.2 706,836 + Pf6N2E2_620 0.74 -2.4 706,836 + Pf6N2E2_620 0.74 -4.1 706,836 + Pf6N2E2_620 0.74 -3.5 706,836 + Pf6N2E2_620 0.74 -2.8 706,836 + Pf6N2E2_620 0.74 -0.1 706,836 + Pf6N2E2_620 0.74 -3.5 706,836 + Pf6N2E2_620 0.74 -0.5 706,836 + Pf6N2E2_620 0.74 -3.1 706,836 + Pf6N2E2_620 0.74 -3.0 706,837 - Pf6N2E2_620 0.74 -0.5 706,837 - Pf6N2E2_620 0.74 -0.5 706,837 - Pf6N2E2_620 0.74 -1.6 706,837 - Pf6N2E2_620 0.74 -2.1 707,247 + -3.3 707,247 + -2.8 707,248 - -4.2 707,248 - -1.9 707,313 + -4.2 707,458 + Pf6N2E2_621 0.11 -2.9 707,458 + Pf6N2E2_621 0.11 -1.8 707,458 + Pf6N2E2_621 0.11 -3.7 707,459 - Pf6N2E2_621 0.11 -3.9 707,723 + Pf6N2E2_621 0.26 -1.4 707,723 + Pf6N2E2_621 0.26 -2.7 707,723 + Pf6N2E2_621 0.26 -2.8 707,723 + Pf6N2E2_621 0.26 -2.6 707,780 + Pf6N2E2_621 0.30 -4.0 707,780 + Pf6N2E2_621 0.30 -2.4 707,781 - Pf6N2E2_621 0.30 -1.1 707,995 + Pf6N2E2_621 0.42 -1.1 707,995 + Pf6N2E2_621 0.42 -2.3 707,996 - Pf6N2E2_621 0.42 -3.1
Or see this region's nucleotide sequence