Experiment: Spectinomycin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_612 and Pf6N2E2_613 are separated by 118 nucleotides Pf6N2E2_613 and Pf6N2E2_614 overlap by 14 nucleotides
Pf6N2E2_612 - Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26) (from data) , at 695,832 to 697,412
_612
Pf6N2E2_613 - lipoprotein, putative, at 697,531 to 697,869
_613
Pf6N2E2_614 - 3-carboxymuconate cyclase, at 697,856 to 699,031
_614
Position (kb)
697
698 Strain fitness (log2 ratio)
-1
0
1 at 696.735 kb on - strand, within Pf6N2E2_612 at 696.858 kb on + strand, within Pf6N2E2_612 at 697.705 kb on - strand, within Pf6N2E2_613 at 697.985 kb on + strand, within Pf6N2E2_614 at 697.986 kb on - strand, within Pf6N2E2_614 at 697.986 kb on - strand, within Pf6N2E2_614 at 697.986 kb on - strand, within Pf6N2E2_614 at 697.986 kb on - strand, within Pf6N2E2_614 at 698.153 kb on + strand, within Pf6N2E2_614 at 698.153 kb on + strand, within Pf6N2E2_614 at 698.266 kb on + strand, within Pf6N2E2_614 at 698.266 kb on + strand, within Pf6N2E2_614 at 698.270 kb on + strand, within Pf6N2E2_614 at 698.270 kb on + strand, within Pf6N2E2_614 at 698.270 kb on + strand, within Pf6N2E2_614 at 698.271 kb on - strand, within Pf6N2E2_614 at 698.271 kb on - strand, within Pf6N2E2_614 at 698.438 kb on + strand, within Pf6N2E2_614 at 698.438 kb on + strand, within Pf6N2E2_614 at 698.438 kb on + strand, within Pf6N2E2_614 at 698.438 kb on + strand, within Pf6N2E2_614 at 698.439 kb on - strand, within Pf6N2E2_614 at 698.439 kb on - strand, within Pf6N2E2_614 at 698.439 kb on - strand, within Pf6N2E2_614 at 698.439 kb on - strand, within Pf6N2E2_614 at 698.439 kb on - strand, within Pf6N2E2_614 at 698.439 kb on - strand, within Pf6N2E2_614 at 698.472 kb on + strand, within Pf6N2E2_614 at 698.472 kb on + strand, within Pf6N2E2_614 at 698.472 kb on + strand, within Pf6N2E2_614 at 698.472 kb on + strand, within Pf6N2E2_614 at 698.472 kb on + strand, within Pf6N2E2_614 at 698.473 kb on - strand, within Pf6N2E2_614 at 698.473 kb on - strand, within Pf6N2E2_614 at 698.756 kb on + strand, within Pf6N2E2_614 at 698.756 kb on + strand, within Pf6N2E2_614
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.01 mg/ml remove 696,735 - Pf6N2E2_612 0.57 -0.2 696,858 + Pf6N2E2_612 0.65 +0.5 697,705 - Pf6N2E2_613 0.51 -0.8 697,985 + Pf6N2E2_614 0.11 +0.5 697,986 - Pf6N2E2_614 0.11 -0.0 697,986 - Pf6N2E2_614 0.11 -1.2 697,986 - Pf6N2E2_614 0.11 +0.7 697,986 - Pf6N2E2_614 0.11 +0.5 698,153 + Pf6N2E2_614 0.25 +0.7 698,153 + Pf6N2E2_614 0.25 +1.4 698,266 + Pf6N2E2_614 0.35 -1.5 698,266 + Pf6N2E2_614 0.35 +0.3 698,270 + Pf6N2E2_614 0.35 +1.7 698,270 + Pf6N2E2_614 0.35 +0.3 698,270 + Pf6N2E2_614 0.35 +0.3 698,271 - Pf6N2E2_614 0.35 -1.3 698,271 - Pf6N2E2_614 0.35 -0.6 698,438 + Pf6N2E2_614 0.49 -0.3 698,438 + Pf6N2E2_614 0.49 -0.2 698,438 + Pf6N2E2_614 0.49 -0.7 698,438 + Pf6N2E2_614 0.49 -0.3 698,439 - Pf6N2E2_614 0.50 +0.3 698,439 - Pf6N2E2_614 0.50 +0.3 698,439 - Pf6N2E2_614 0.50 -0.5 698,439 - Pf6N2E2_614 0.50 -0.3 698,439 - Pf6N2E2_614 0.50 -0.4 698,439 - Pf6N2E2_614 0.50 +1.3 698,472 + Pf6N2E2_614 0.52 +1.3 698,472 + Pf6N2E2_614 0.52 -0.4 698,472 + Pf6N2E2_614 0.52 +0.5 698,472 + Pf6N2E2_614 0.52 -0.6 698,472 + Pf6N2E2_614 0.52 -0.4 698,473 - Pf6N2E2_614 0.52 +0.4 698,473 - Pf6N2E2_614 0.52 +1.5 698,756 + Pf6N2E2_614 0.77 +0.2 698,756 + Pf6N2E2_614 0.77 -0.2
Or see this region's nucleotide sequence