Experiment: Spectinomycin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_601 and Pf6N2E2_602 are separated by 45 nucleotides Pf6N2E2_602 and Pf6N2E2_603 overlap by 4 nucleotides Pf6N2E2_603 and Pf6N2E2_604 are separated by 13 nucleotides
Pf6N2E2_601 - GlpG protein (membrane protein of glp regulon), at 676,097 to 676,969
_601
Pf6N2E2_602 - FIG00954117: hypothetical protein, at 677,015 to 677,275
_602
Pf6N2E2_603 - FIG00953934: hypothetical protein, at 677,272 to 678,102
_603
Pf6N2E2_604 - Membrane alanine aminopeptidase N (EC 3.4.11.2), at 678,116 to 680,773
_604
Position (kb) 677
678
679 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 676.475 kb on + strand, within Pf6N2E2_601 at 676.475 kb on + strand, within Pf6N2E2_601 at 676.476 kb on - strand, within Pf6N2E2_601 at 676.476 kb on - strand, within Pf6N2E2_601 at 676.760 kb on + strand, within Pf6N2E2_601 at 676.787 kb on + strand, within Pf6N2E2_601 at 676.954 kb on + strand at 677.060 kb on + strand, within Pf6N2E2_602 at 677.199 kb on - strand, within Pf6N2E2_602 at 677.199 kb on - strand, within Pf6N2E2_602 at 677.338 kb on + strand at 677.339 kb on - strand at 677.408 kb on - strand, within Pf6N2E2_603 at 677.606 kb on - strand, within Pf6N2E2_603 at 677.606 kb on - strand, within Pf6N2E2_603 at 677.606 kb on - strand, within Pf6N2E2_603 at 677.606 kb on - strand, within Pf6N2E2_603 at 678.031 kb on + strand at 678.032 kb on - strand at 678.061 kb on - strand at 678.067 kb on - strand at 678.070 kb on + strand at 678.070 kb on + strand at 678.070 kb on + strand at 678.071 kb on - strand at 678.071 kb on - strand at 678.071 kb on - strand at 678.080 kb on - strand at 678.100 kb on + strand at 678.101 kb on - strand at 678.101 kb on - strand at 678.101 kb on - strand at 678.101 kb on - strand at 678.143 kb on + strand at 678.155 kb on + strand at 678.170 kb on + strand at 678.171 kb on - strand at 678.353 kb on + strand at 678.354 kb on - strand at 678.462 kb on - strand, within Pf6N2E2_604 at 678.462 kb on - strand, within Pf6N2E2_604 at 678.521 kb on + strand, within Pf6N2E2_604 at 678.521 kb on + strand, within Pf6N2E2_604 at 678.522 kb on - strand, within Pf6N2E2_604 at 678.525 kb on - strand, within Pf6N2E2_604 at 678.525 kb on - strand, within Pf6N2E2_604 at 678.686 kb on + strand, within Pf6N2E2_604 at 678.686 kb on + strand, within Pf6N2E2_604 at 678.687 kb on - strand, within Pf6N2E2_604 at 678.687 kb on - strand, within Pf6N2E2_604 at 678.773 kb on + strand, within Pf6N2E2_604 at 678.773 kb on + strand, within Pf6N2E2_604 at 678.773 kb on + strand, within Pf6N2E2_604 at 678.774 kb on - strand, within Pf6N2E2_604 at 678.774 kb on - strand, within Pf6N2E2_604 at 678.852 kb on + strand, within Pf6N2E2_604 at 678.853 kb on - strand, within Pf6N2E2_604 at 678.854 kb on + strand, within Pf6N2E2_604 at 678.855 kb on - strand, within Pf6N2E2_604 at 678.855 kb on - strand, within Pf6N2E2_604 at 678.866 kb on + strand, within Pf6N2E2_604 at 678.884 kb on - strand, within Pf6N2E2_604 at 678.884 kb on - strand, within Pf6N2E2_604 at 678.884 kb on - strand at 678.940 kb on + strand, within Pf6N2E2_604 at 678.941 kb on - strand, within Pf6N2E2_604 at 678.941 kb on - strand, within Pf6N2E2_604 at 679.028 kb on + strand, within Pf6N2E2_604 at 679.028 kb on + strand, within Pf6N2E2_604
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.01 mg/ml remove 676,475 + Pf6N2E2_601 0.43 +0.5 676,475 + Pf6N2E2_601 0.43 +0.2 676,476 - Pf6N2E2_601 0.43 -0.4 676,476 - Pf6N2E2_601 0.43 -0.7 676,760 + Pf6N2E2_601 0.76 +0.1 676,787 + Pf6N2E2_601 0.79 -1.3 676,954 + -0.2 677,060 + Pf6N2E2_602 0.17 +0.4 677,199 - Pf6N2E2_602 0.70 +0.5 677,199 - Pf6N2E2_602 0.70 +0.5 677,338 + +1.5 677,339 - +0.7 677,408 - Pf6N2E2_603 0.16 -0.5 677,606 - Pf6N2E2_603 0.40 -0.8 677,606 - Pf6N2E2_603 0.40 +0.4 677,606 - Pf6N2E2_603 0.40 -0.9 677,606 - Pf6N2E2_603 0.40 -0.6 678,031 + +1.2 678,032 - +1.8 678,061 - -0.2 678,067 - +0.5 678,070 + -0.8 678,070 + +0.5 678,070 + +0.1 678,071 - -0.2 678,071 - -0.3 678,071 - +0.2 678,080 - +0.2 678,100 + -0.8 678,101 - -0.3 678,101 - -0.5 678,101 - +1.8 678,101 - +0.5 678,143 + -1.5 678,155 + +0.6 678,170 + +0.3 678,171 - -1.6 678,353 + +0.1 678,354 - -0.1 678,462 - Pf6N2E2_604 0.13 +0.0 678,462 - Pf6N2E2_604 0.13 +1.3 678,521 + Pf6N2E2_604 0.15 +0.6 678,521 + Pf6N2E2_604 0.15 +0.4 678,522 - Pf6N2E2_604 0.15 -0.3 678,525 - Pf6N2E2_604 0.15 -0.1 678,525 - Pf6N2E2_604 0.15 -0.8 678,686 + Pf6N2E2_604 0.21 -1.1 678,686 + Pf6N2E2_604 0.21 -0.5 678,687 - Pf6N2E2_604 0.21 -0.2 678,687 - Pf6N2E2_604 0.21 -0.4 678,773 + Pf6N2E2_604 0.25 +1.2 678,773 + Pf6N2E2_604 0.25 +0.3 678,773 + Pf6N2E2_604 0.25 -1.2 678,774 - Pf6N2E2_604 0.25 +2.1 678,774 - Pf6N2E2_604 0.25 -0.1 678,852 + Pf6N2E2_604 0.28 +0.1 678,853 - Pf6N2E2_604 0.28 +0.0 678,854 + Pf6N2E2_604 0.28 +0.5 678,855 - Pf6N2E2_604 0.28 +0.1 678,855 - Pf6N2E2_604 0.28 +0.1 678,866 + Pf6N2E2_604 0.28 +0.0 678,884 - Pf6N2E2_604 0.29 +1.2 678,884 - Pf6N2E2_604 0.29 +1.3 678,884 - +1.8 678,940 + Pf6N2E2_604 0.31 -2.1 678,941 - Pf6N2E2_604 0.31 -0.2 678,941 - Pf6N2E2_604 0.31 +2.8 679,028 + Pf6N2E2_604 0.34 -0.1 679,028 + Pf6N2E2_604 0.34 +1.5
Or see this region's nucleotide sequence