Experiment: Spectinomycin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5188 and Pf6N2E2_5189 are separated by 51 nucleotides Pf6N2E2_5189 and Pf6N2E2_5190 are separated by 132 nucleotides Pf6N2E2_5190 and Pf6N2E2_5191 are separated by 10 nucleotides Pf6N2E2_5191 and Pf6N2E2_5192 are separated by 179 nucleotides
Pf6N2E2_5188 - Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-), at 5,984,722 to 5,985,174
_5188
Pf6N2E2_5189 - Phosphotransferase system, HPr-related proteins, at 5,985,226 to 5,985,630
_5189
Pf6N2E2_5190 - Carbonic anhydrase (EC 4.2.1.1), at 5,985,763 to 5,986,407
_5190
Pf6N2E2_5191 - Conserved domain protein, at 5,986,418 to 5,986,945
_5191
Pf6N2E2_5192 - FIG00953119: hypothetical protein, at 5,987,125 to 5,988,285
_5192
Position (kb)
5985
5986
5987 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5984.788 kb on + strand, within Pf6N2E2_5188 at 5984.788 kb on + strand, within Pf6N2E2_5188 at 5984.797 kb on + strand, within Pf6N2E2_5188 at 5984.798 kb on - strand, within Pf6N2E2_5188 at 5984.846 kb on - strand, within Pf6N2E2_5188 at 5985.004 kb on + strand, within Pf6N2E2_5188 at 5985.004 kb on + strand, within Pf6N2E2_5188 at 5985.004 kb on + strand, within Pf6N2E2_5188 at 5985.005 kb on - strand, within Pf6N2E2_5188 at 5985.005 kb on - strand, within Pf6N2E2_5188 at 5985.070 kb on + strand, within Pf6N2E2_5188 at 5985.070 kb on + strand, within Pf6N2E2_5188 at 5985.112 kb on + strand, within Pf6N2E2_5188 at 5985.295 kb on - strand, within Pf6N2E2_5189 at 5985.643 kb on + strand at 5985.644 kb on - strand at 5985.655 kb on + strand at 5985.655 kb on + strand at 5985.656 kb on - strand at 5985.742 kb on + strand at 5985.742 kb on + strand at 5985.742 kb on + strand at 5985.742 kb on + strand at 5985.773 kb on + strand at 5985.773 kb on + strand at 5985.773 kb on + strand at 5985.774 kb on - strand at 5985.790 kb on - strand at 5985.871 kb on + strand, within Pf6N2E2_5190 at 5985.947 kb on + strand, within Pf6N2E2_5190 at 5985.947 kb on + strand, within Pf6N2E2_5190 at 5985.947 kb on + strand, within Pf6N2E2_5190 at 5985.947 kb on + strand, within Pf6N2E2_5190 at 5985.948 kb on - strand, within Pf6N2E2_5190 at 5985.948 kb on - strand, within Pf6N2E2_5190 at 5985.948 kb on - strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.968 kb on + strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5985.969 kb on - strand, within Pf6N2E2_5190 at 5986.141 kb on + strand, within Pf6N2E2_5190 at 5986.142 kb on - strand, within Pf6N2E2_5190 at 5986.142 kb on - strand, within Pf6N2E2_5190 at 5986.142 kb on - strand, within Pf6N2E2_5190 at 5986.142 kb on - strand, within Pf6N2E2_5190 at 5986.144 kb on + strand, within Pf6N2E2_5190 at 5986.144 kb on + strand, within Pf6N2E2_5190 at 5986.144 kb on + strand, within Pf6N2E2_5190 at 5986.144 kb on + strand, within Pf6N2E2_5190 at 5986.145 kb on - strand, within Pf6N2E2_5190 at 5986.145 kb on - strand, within Pf6N2E2_5190 at 5986.145 kb on - strand, within Pf6N2E2_5190 at 5986.245 kb on + strand, within Pf6N2E2_5190 at 5986.245 kb on + strand, within Pf6N2E2_5190 at 5986.306 kb on + strand, within Pf6N2E2_5190 at 5986.306 kb on + strand, within Pf6N2E2_5190 at 5986.307 kb on - strand, within Pf6N2E2_5190 at 5986.307 kb on - strand, within Pf6N2E2_5190 at 5986.307 kb on - strand, within Pf6N2E2_5190 at 5986.516 kb on - strand, within Pf6N2E2_5191 at 5986.516 kb on - strand, within Pf6N2E2_5191 at 5986.516 kb on - strand, within Pf6N2E2_5191 at 5986.545 kb on + strand, within Pf6N2E2_5191 at 5986.569 kb on + strand, within Pf6N2E2_5191 at 5986.643 kb on + strand, within Pf6N2E2_5191 at 5986.643 kb on + strand, within Pf6N2E2_5191 at 5986.643 kb on + strand, within Pf6N2E2_5191 at 5986.644 kb on - strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.692 kb on + strand, within Pf6N2E2_5191 at 5986.693 kb on - strand, within Pf6N2E2_5191 at 5986.693 kb on - strand, within Pf6N2E2_5191 at 5986.693 kb on - strand, within Pf6N2E2_5191 at 5986.693 kb on - strand, within Pf6N2E2_5191 at 5986.693 kb on - strand, within Pf6N2E2_5191 at 5986.693 kb on - strand, within Pf6N2E2_5191 at 5986.693 kb on - strand, within Pf6N2E2_5191 at 5986.693 kb on - strand, within Pf6N2E2_5191 at 5986.693 kb on - strand, within Pf6N2E2_5191 at 5986.693 kb on - strand, within Pf6N2E2_5191 at 5986.693 kb on - strand, within Pf6N2E2_5191 at 5986.693 kb on - strand, within Pf6N2E2_5191 at 5986.894 kb on - strand at 5986.894 kb on - strand at 5986.939 kb on + strand at 5986.939 kb on + strand at 5986.939 kb on + strand at 5986.940 kb on - strand at 5986.964 kb on + strand at 5987.022 kb on + strand at 5987.022 kb on + strand at 5987.023 kb on - strand at 5987.050 kb on + strand at 5987.050 kb on + strand at 5987.050 kb on + strand at 5987.050 kb on + strand at 5987.050 kb on + strand at 5987.050 kb on + strand at 5987.050 kb on + strand at 5987.051 kb on - strand at 5987.051 kb on - strand at 5987.051 kb on - strand at 5987.051 kb on - strand at 5987.051 kb on - strand at 5987.051 kb on - strand at 5987.051 kb on - strand at 5987.156 kb on - strand at 5987.243 kb on + strand, within Pf6N2E2_5192 at 5987.243 kb on + strand, within Pf6N2E2_5192 at 5987.243 kb on + strand, within Pf6N2E2_5192 at 5987.243 kb on + strand, within Pf6N2E2_5192
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.01 mg/ml remove 5,984,788 + Pf6N2E2_5188 0.15 +0.3 5,984,788 + Pf6N2E2_5188 0.15 +2.1 5,984,797 + Pf6N2E2_5188 0.17 -0.0 5,984,798 - Pf6N2E2_5188 0.17 +1.2 5,984,846 - Pf6N2E2_5188 0.27 +1.2 5,985,004 + Pf6N2E2_5188 0.62 +0.2 5,985,004 + Pf6N2E2_5188 0.62 +0.0 5,985,004 + Pf6N2E2_5188 0.62 -0.2 5,985,005 - Pf6N2E2_5188 0.62 -0.3 5,985,005 - Pf6N2E2_5188 0.62 -0.8 5,985,070 + Pf6N2E2_5188 0.77 +0.1 5,985,070 + Pf6N2E2_5188 0.77 +0.6 5,985,112 + Pf6N2E2_5188 0.86 -1.5 5,985,295 - Pf6N2E2_5189 0.17 -0.8 5,985,643 + +0.4 5,985,644 - +0.1 5,985,655 + +0.1 5,985,655 + -0.9 5,985,656 - +0.2 5,985,742 + -0.3 5,985,742 + +0.2 5,985,742 + +0.7 5,985,742 + +0.1 5,985,773 + -1.4 5,985,773 + -0.9 5,985,773 + -0.1 5,985,774 - -1.2 5,985,790 - -1.1 5,985,871 + Pf6N2E2_5190 0.17 -1.8 5,985,947 + Pf6N2E2_5190 0.29 -0.7 5,985,947 + Pf6N2E2_5190 0.29 -1.0 5,985,947 + Pf6N2E2_5190 0.29 -0.8 5,985,947 + Pf6N2E2_5190 0.29 -1.6 5,985,948 - Pf6N2E2_5190 0.29 -1.4 5,985,948 - Pf6N2E2_5190 0.29 +1.1 5,985,948 - Pf6N2E2_5190 0.29 -1.5 5,985,968 + Pf6N2E2_5190 0.32 -1.2 5,985,968 + Pf6N2E2_5190 0.32 -2.0 5,985,968 + Pf6N2E2_5190 0.32 -2.3 5,985,968 + Pf6N2E2_5190 0.32 -0.7 5,985,968 + Pf6N2E2_5190 0.32 -1.9 5,985,968 + Pf6N2E2_5190 0.32 -2.0 5,985,968 + Pf6N2E2_5190 0.32 -0.6 5,985,969 - Pf6N2E2_5190 0.32 -2.2 5,985,969 - Pf6N2E2_5190 0.32 -0.3 5,985,969 - Pf6N2E2_5190 0.32 -0.6 5,985,969 - Pf6N2E2_5190 0.32 +2.1 5,985,969 - Pf6N2E2_5190 0.32 -1.0 5,985,969 - Pf6N2E2_5190 0.32 -0.7 5,985,969 - Pf6N2E2_5190 0.32 -1.6 5,985,969 - Pf6N2E2_5190 0.32 -1.9 5,986,141 + Pf6N2E2_5190 0.59 -2.5 5,986,142 - Pf6N2E2_5190 0.59 -1.0 5,986,142 - Pf6N2E2_5190 0.59 -1.5 5,986,142 - Pf6N2E2_5190 0.59 +0.2 5,986,142 - Pf6N2E2_5190 0.59 -0.2 5,986,144 + Pf6N2E2_5190 0.59 -0.1 5,986,144 + Pf6N2E2_5190 0.59 -1.2 5,986,144 + Pf6N2E2_5190 0.59 -1.6 5,986,144 + Pf6N2E2_5190 0.59 -0.5 5,986,145 - Pf6N2E2_5190 0.59 -1.5 5,986,145 - Pf6N2E2_5190 0.59 -0.8 5,986,145 - Pf6N2E2_5190 0.59 -0.9 5,986,245 + Pf6N2E2_5190 0.75 -1.0 5,986,245 + Pf6N2E2_5190 0.75 -1.0 5,986,306 + Pf6N2E2_5190 0.84 -1.9 5,986,306 + Pf6N2E2_5190 0.84 -1.4 5,986,307 - Pf6N2E2_5190 0.84 -0.6 5,986,307 - Pf6N2E2_5190 0.84 -2.3 5,986,307 - Pf6N2E2_5190 0.84 +0.1 5,986,516 - Pf6N2E2_5191 0.19 -0.4 5,986,516 - Pf6N2E2_5191 0.19 +0.5 5,986,516 - Pf6N2E2_5191 0.19 +0.6 5,986,545 + Pf6N2E2_5191 0.24 +1.2 5,986,569 + Pf6N2E2_5191 0.29 -0.2 5,986,643 + Pf6N2E2_5191 0.43 +0.4 5,986,643 + Pf6N2E2_5191 0.43 +0.2 5,986,643 + Pf6N2E2_5191 0.43 +0.5 5,986,644 - Pf6N2E2_5191 0.43 +0.1 5,986,692 + Pf6N2E2_5191 0.52 -0.3 5,986,692 + Pf6N2E2_5191 0.52 +0.6 5,986,692 + Pf6N2E2_5191 0.52 -0.4 5,986,692 + Pf6N2E2_5191 0.52 -0.1 5,986,692 + Pf6N2E2_5191 0.52 -0.0 5,986,692 + Pf6N2E2_5191 0.52 -0.1 5,986,692 + Pf6N2E2_5191 0.52 -0.1 5,986,692 + Pf6N2E2_5191 0.52 +0.2 5,986,692 + Pf6N2E2_5191 0.52 +0.4 5,986,692 + Pf6N2E2_5191 0.52 +0.2 5,986,692 + Pf6N2E2_5191 0.52 -0.6 5,986,692 + Pf6N2E2_5191 0.52 +0.2 5,986,692 + Pf6N2E2_5191 0.52 +0.2 5,986,692 + Pf6N2E2_5191 0.52 +0.2 5,986,692 + Pf6N2E2_5191 0.52 +0.1 5,986,692 + Pf6N2E2_5191 0.52 -0.4 5,986,692 + Pf6N2E2_5191 0.52 +0.6 5,986,692 + Pf6N2E2_5191 0.52 +1.0 5,986,692 + Pf6N2E2_5191 0.52 +0.4 5,986,692 + Pf6N2E2_5191 0.52 -1.2 5,986,692 + Pf6N2E2_5191 0.52 +0.3 5,986,693 - Pf6N2E2_5191 0.52 -0.7 5,986,693 - Pf6N2E2_5191 0.52 +0.7 5,986,693 - Pf6N2E2_5191 0.52 +0.8 5,986,693 - Pf6N2E2_5191 0.52 +0.8 5,986,693 - Pf6N2E2_5191 0.52 -1.5 5,986,693 - Pf6N2E2_5191 0.52 -0.9 5,986,693 - Pf6N2E2_5191 0.52 +0.3 5,986,693 - Pf6N2E2_5191 0.52 -0.9 5,986,693 - Pf6N2E2_5191 0.52 +0.1 5,986,693 - Pf6N2E2_5191 0.52 -0.4 5,986,693 - Pf6N2E2_5191 0.52 -0.2 5,986,693 - Pf6N2E2_5191 0.52 -2.5 5,986,894 - +0.5 5,986,894 - -0.4 5,986,939 + -0.1 5,986,939 + +0.1 5,986,939 + +0.6 5,986,940 - +0.5 5,986,964 + -0.1 5,987,022 + -0.2 5,987,022 + -1.3 5,987,023 - +0.2 5,987,050 + -0.5 5,987,050 + +0.7 5,987,050 + +0.3 5,987,050 + -0.7 5,987,050 + -0.8 5,987,050 + -0.5 5,987,050 + +0.2 5,987,051 - -0.9 5,987,051 - +0.6 5,987,051 - +0.6 5,987,051 - -0.0 5,987,051 - -0.7 5,987,051 - -0.1 5,987,051 - +0.4 5,987,156 - +0.2 5,987,243 + Pf6N2E2_5192 0.10 -0.3 5,987,243 + Pf6N2E2_5192 0.10 +1.2 5,987,243 + Pf6N2E2_5192 0.10 -1.1 5,987,243 + Pf6N2E2_5192 0.10 -1.5
Or see this region's nucleotide sequence