Experiment: Spectinomycin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4597 and Pf6N2E2_4598 are separated by 309 nucleotides Pf6N2E2_4598 and Pf6N2E2_4599 are separated by 5 nucleotides Pf6N2E2_4599 and Pf6N2E2_4600 are separated by 10 nucleotides Pf6N2E2_4600 and Pf6N2E2_4601 are separated by 35 nucleotides
Pf6N2E2_4597 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 5,323,994 to 5,325,085
_4597
Pf6N2E2_4598 - COG1872, at 5,325,395 to 5,325,688
_4598
Pf6N2E2_4599 - Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock), at 5,325,694 to 5,326,284
_4599
Pf6N2E2_4600 - Pyrroline-5-carboxylate reductase (EC 1.5.1.2), at 5,326,295 to 5,327,113
_4600
Pf6N2E2_4601 - Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC, at 5,327,149 to 5,327,835
_4601
Position (kb) 5325
5326
5327 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5325.009 kb on + strand at 5325.018 kb on + strand at 5325.018 kb on + strand at 5325.019 kb on - strand at 5325.019 kb on - strand at 5325.019 kb on - strand at 5325.019 kb on - strand at 5325.019 kb on - strand at 5325.030 kb on + strand at 5325.031 kb on - strand at 5325.031 kb on - strand at 5325.031 kb on - strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.161 kb on + strand at 5325.162 kb on - strand at 5325.167 kb on + strand at 5325.168 kb on - strand at 5325.314 kb on + strand at 5325.314 kb on + strand at 5325.314 kb on + strand at 5325.314 kb on + strand at 5325.314 kb on + strand at 5325.314 kb on + strand at 5325.314 kb on + strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.315 kb on - strand at 5325.318 kb on + strand at 5325.318 kb on + strand at 5325.318 kb on + strand at 5325.318 kb on + strand at 5325.319 kb on - strand at 5325.319 kb on - strand at 5325.446 kb on + strand, within Pf6N2E2_4598 at 5325.446 kb on + strand, within Pf6N2E2_4598 at 5325.446 kb on + strand, within Pf6N2E2_4598 at 5325.446 kb on + strand, within Pf6N2E2_4598 at 5325.446 kb on + strand, within Pf6N2E2_4598 at 5325.447 kb on - strand, within Pf6N2E2_4598 at 5325.447 kb on - strand, within Pf6N2E2_4598 at 5325.447 kb on - strand, within Pf6N2E2_4598 at 5325.447 kb on - strand, within Pf6N2E2_4598 at 5325.447 kb on - strand, within Pf6N2E2_4598 at 5325.681 kb on + strand at 5325.682 kb on - strand at 5325.682 kb on - strand at 5325.682 kb on - strand at 5325.682 kb on - strand at 5325.682 kb on - strand at 5325.682 kb on - strand at 5325.682 kb on - strand at 5325.828 kb on + strand, within Pf6N2E2_4599 at 5325.828 kb on + strand, within Pf6N2E2_4599 at 5325.829 kb on - strand, within Pf6N2E2_4599 at 5326.020 kb on - strand, within Pf6N2E2_4599 at 5326.175 kb on + strand, within Pf6N2E2_4599 at 5326.175 kb on + strand, within Pf6N2E2_4599 at 5326.175 kb on + strand, within Pf6N2E2_4599 at 5326.175 kb on + strand, within Pf6N2E2_4599 at 5326.288 kb on + strand at 5326.288 kb on + strand at 5326.288 kb on + strand at 5326.288 kb on + strand at 5326.289 kb on - strand at 5326.289 kb on - strand at 5326.289 kb on - strand at 5326.289 kb on - strand at 5326.296 kb on + strand at 5327.125 kb on - strand at 5327.125 kb on - strand at 5327.125 kb on - strand at 5327.125 kb on - strand at 5327.125 kb on - strand at 5327.127 kb on - strand at 5327.127 kb on - strand at 5327.127 kb on - strand at 5327.127 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.01 mg/ml remove 5,325,009 + -0.1 5,325,018 + +0.5 5,325,018 + +1.9 5,325,019 - -0.8 5,325,019 - -0.6 5,325,019 - -0.6 5,325,019 - -1.3 5,325,019 - -0.5 5,325,030 + -0.6 5,325,031 - +0.3 5,325,031 - -0.2 5,325,031 - +1.0 5,325,161 + -0.6 5,325,161 + +0.8 5,325,161 + +1.1 5,325,161 + -0.1 5,325,161 + +0.8 5,325,161 + -0.1 5,325,161 + -0.5 5,325,161 + +0.6 5,325,161 + +0.2 5,325,162 - +0.2 5,325,167 + -0.4 5,325,168 - -0.6 5,325,314 + +0.2 5,325,314 + -0.6 5,325,314 + -0.5 5,325,314 + -0.6 5,325,314 + +0.1 5,325,314 + -0.9 5,325,314 + -0.5 5,325,315 - +0.0 5,325,315 - +0.8 5,325,315 - -1.4 5,325,315 - +0.4 5,325,315 - +0.8 5,325,315 - +0.9 5,325,315 - -0.8 5,325,315 - +0.6 5,325,315 - +0.0 5,325,318 + +0.2 5,325,318 + -0.8 5,325,318 + +0.5 5,325,318 + +0.9 5,325,319 - +0.8 5,325,319 - -0.5 5,325,446 + Pf6N2E2_4598 0.17 -0.1 5,325,446 + Pf6N2E2_4598 0.17 -0.5 5,325,446 + Pf6N2E2_4598 0.17 -0.5 5,325,446 + Pf6N2E2_4598 0.17 +0.4 5,325,446 + Pf6N2E2_4598 0.17 +0.6 5,325,447 - Pf6N2E2_4598 0.18 +0.1 5,325,447 - Pf6N2E2_4598 0.18 +0.7 5,325,447 - Pf6N2E2_4598 0.18 +0.7 5,325,447 - Pf6N2E2_4598 0.18 +0.2 5,325,447 - Pf6N2E2_4598 0.18 -0.1 5,325,681 + +0.7 5,325,682 - -0.9 5,325,682 - -0.1 5,325,682 - +0.1 5,325,682 - -0.5 5,325,682 - -0.7 5,325,682 - -0.6 5,325,682 - -0.5 5,325,828 + Pf6N2E2_4599 0.23 -2.3 5,325,828 + Pf6N2E2_4599 0.23 +0.2 5,325,829 - Pf6N2E2_4599 0.23 -1.8 5,326,020 - Pf6N2E2_4599 0.55 -1.6 5,326,175 + Pf6N2E2_4599 0.81 -1.3 5,326,175 + Pf6N2E2_4599 0.81 -2.5 5,326,175 + Pf6N2E2_4599 0.81 -1.6 5,326,175 + Pf6N2E2_4599 0.81 -2.0 5,326,288 + -1.6 5,326,288 + -2.1 5,326,288 + -4.2 5,326,288 + -2.3 5,326,289 - -1.2 5,326,289 - -0.5 5,326,289 - -0.6 5,326,289 - -1.4 5,326,296 + -1.9 5,327,125 - -0.3 5,327,125 - -0.6 5,327,125 - -0.3 5,327,125 - -0.2 5,327,125 - +0.3 5,327,127 - -0.2 5,327,127 - -0.1 5,327,127 - -0.7 5,327,127 - -0.5
Or see this region's nucleotide sequence