Experiment: Spectinomycin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4532 and Pf6N2E2_4533 overlap by 14 nucleotides Pf6N2E2_4533 and Pf6N2E2_4534 are separated by 115 nucleotides Pf6N2E2_4534 and Pf6N2E2_4535 overlap by 4 nucleotides
Pf6N2E2_4532 - Flagellum-specific ATP synthase FliI, at 5,266,996 to 5,268,351
_4532
Pf6N2E2_4533 - RspO, at 5,268,338 to 5,268,757
_4533
Pf6N2E2_4534 - hypothetical protein, at 5,268,873 to 5,269,319
_4534
Pf6N2E2_4535 - Type III secretion inner membrane protein (YscQ,homologous to flagellar export components), at 5,269,316 to 5,270,419
_4535
Position (kb) 5268
5269
5270 Strain fitness (log2 ratio) -1
0
1 at 5267.885 kb on - strand, within Pf6N2E2_4532 at 5267.950 kb on + strand, within Pf6N2E2_4532 at 5267.951 kb on - strand, within Pf6N2E2_4532 at 5267.951 kb on - strand, within Pf6N2E2_4532 at 5267.952 kb on + strand, within Pf6N2E2_4532 at 5267.953 kb on - strand, within Pf6N2E2_4532 at 5267.953 kb on - strand, within Pf6N2E2_4532 at 5267.953 kb on - strand, within Pf6N2E2_4532 at 5267.953 kb on - strand, within Pf6N2E2_4532 at 5268.003 kb on - strand, within Pf6N2E2_4532 at 5268.337 kb on + strand at 5268.337 kb on + strand at 5268.337 kb on + strand at 5268.338 kb on - strand at 5268.728 kb on + strand at 5268.832 kb on - strand at 5268.939 kb on + strand, within Pf6N2E2_4534 at 5269.208 kb on - strand, within Pf6N2E2_4534 at 5269.208 kb on - strand, within Pf6N2E2_4534 at 5269.208 kb on - strand, within Pf6N2E2_4534 at 5269.501 kb on + strand, within Pf6N2E2_4535 at 5269.502 kb on - strand, within Pf6N2E2_4535 at 5269.502 kb on - strand, within Pf6N2E2_4535 at 5269.502 kb on - strand, within Pf6N2E2_4535 at 5269.502 kb on - strand, within Pf6N2E2_4535 at 5269.645 kb on + strand, within Pf6N2E2_4535 at 5269.646 kb on - strand, within Pf6N2E2_4535 at 5270.136 kb on - strand, within Pf6N2E2_4535
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.01 mg/ml remove 5,267,885 - Pf6N2E2_4532 0.66 +0.1 5,267,950 + Pf6N2E2_4532 0.70 +0.1 5,267,951 - Pf6N2E2_4532 0.70 +0.2 5,267,951 - Pf6N2E2_4532 0.70 +0.8 5,267,952 + Pf6N2E2_4532 0.71 +0.1 5,267,953 - Pf6N2E2_4532 0.71 +0.7 5,267,953 - Pf6N2E2_4532 0.71 +0.1 5,267,953 - Pf6N2E2_4532 0.71 +0.4 5,267,953 - Pf6N2E2_4532 0.71 -0.2 5,268,003 - Pf6N2E2_4532 0.74 -0.8 5,268,337 + -0.1 5,268,337 + -0.0 5,268,337 + +0.8 5,268,338 - -0.2 5,268,728 + +0.2 5,268,832 - +0.3 5,268,939 + Pf6N2E2_4534 0.15 -0.3 5,269,208 - Pf6N2E2_4534 0.75 -0.4 5,269,208 - Pf6N2E2_4534 0.75 -1.2 5,269,208 - Pf6N2E2_4534 0.75 -1.3 5,269,501 + Pf6N2E2_4535 0.17 +0.7 5,269,502 - Pf6N2E2_4535 0.17 -0.1 5,269,502 - Pf6N2E2_4535 0.17 -0.2 5,269,502 - Pf6N2E2_4535 0.17 +0.6 5,269,502 - Pf6N2E2_4535 0.17 +0.6 5,269,645 + Pf6N2E2_4535 0.30 -1.1 5,269,646 - Pf6N2E2_4535 0.30 +0.9 5,270,136 - Pf6N2E2_4535 0.74 -0.2
Or see this region's nucleotide sequence