Experiment: Spectinomycin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3943 and Pf6N2E2_3944 are separated by 283 nucleotides Pf6N2E2_3944 and Pf6N2E2_3945 are separated by 68 nucleotides Pf6N2E2_3945 and Pf6N2E2_3946 are separated by 49 nucleotides
Pf6N2E2_3943 - COG3332, at 4,580,799 to 4,581,584
_3943
Pf6N2E2_3944 - Protein of unknown function DUF81, at 4,581,868 to 4,582,668
_3944
Pf6N2E2_3945 - Prolipoprotein diacylglyceryl transferase (EC 2.4.99.-), at 4,582,737 to 4,583,546
_3945
Pf6N2E2_3946 - Thymidylate synthase (EC 2.1.1.45), at 4,583,596 to 4,584,567
_3946
Position (kb)
4581
4582
4583 Strain fitness (log2 ratio)
-1
0
1
2 at 4581.032 kb on + strand, within Pf6N2E2_3943 at 4581.032 kb on + strand, within Pf6N2E2_3943 at 4581.033 kb on - strand, within Pf6N2E2_3943 at 4581.136 kb on + strand, within Pf6N2E2_3943 at 4581.136 kb on + strand, within Pf6N2E2_3943 at 4581.137 kb on - strand, within Pf6N2E2_3943 at 4581.137 kb on - strand, within Pf6N2E2_3943 at 4581.229 kb on + strand, within Pf6N2E2_3943 at 4581.229 kb on + strand, within Pf6N2E2_3943 at 4581.229 kb on + strand, within Pf6N2E2_3943 at 4581.229 kb on + strand, within Pf6N2E2_3943 at 4581.230 kb on - strand, within Pf6N2E2_3943 at 4581.262 kb on + strand, within Pf6N2E2_3943 at 4581.263 kb on - strand, within Pf6N2E2_3943 at 4581.263 kb on - strand, within Pf6N2E2_3943 at 4581.263 kb on - strand, within Pf6N2E2_3943 at 4581.269 kb on + strand, within Pf6N2E2_3943 at 4581.270 kb on - strand, within Pf6N2E2_3943 at 4581.478 kb on + strand, within Pf6N2E2_3943 at 4581.478 kb on + strand, within Pf6N2E2_3943 at 4581.479 kb on - strand, within Pf6N2E2_3943 at 4581.540 kb on + strand at 4581.540 kb on + strand at 4581.540 kb on + strand at 4581.540 kb on + strand at 4581.541 kb on - strand at 4581.541 kb on - strand at 4581.574 kb on + strand at 4581.575 kb on - strand at 4581.575 kb on - strand at 4581.591 kb on + strand at 4581.592 kb on - strand at 4581.640 kb on - strand at 4581.647 kb on - strand at 4581.694 kb on + strand at 4581.760 kb on + strand at 4581.786 kb on - strand at 4581.858 kb on + strand at 4581.859 kb on - strand at 4581.901 kb on + strand at 4581.901 kb on + strand at 4581.902 kb on - strand at 4581.902 kb on - strand at 4582.460 kb on + strand, within Pf6N2E2_3944 at 4582.460 kb on + strand, within Pf6N2E2_3944 at 4582.506 kb on + strand, within Pf6N2E2_3944 at 4582.506 kb on + strand, within Pf6N2E2_3944 at 4582.507 kb on - strand, within Pf6N2E2_3944 at 4582.522 kb on + strand, within Pf6N2E2_3944 at 4582.522 kb on + strand, within Pf6N2E2_3944 at 4582.637 kb on + strand at 4582.637 kb on + strand at 4582.637 kb on + strand at 4582.637 kb on + strand at 4582.637 kb on + strand at 4582.682 kb on + strand at 4582.723 kb on + strand at 4583.511 kb on + strand at 4583.511 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.01 mg/ml remove 4,581,032 + Pf6N2E2_3943 0.30 +2.4 4,581,032 + Pf6N2E2_3943 0.30 +0.7 4,581,033 - Pf6N2E2_3943 0.30 +0.2 4,581,136 + Pf6N2E2_3943 0.43 -0.4 4,581,136 + Pf6N2E2_3943 0.43 -1.7 4,581,137 - Pf6N2E2_3943 0.43 -0.1 4,581,137 - Pf6N2E2_3943 0.43 +0.6 4,581,229 + Pf6N2E2_3943 0.55 -0.2 4,581,229 + Pf6N2E2_3943 0.55 +0.2 4,581,229 + Pf6N2E2_3943 0.55 -0.2 4,581,229 + Pf6N2E2_3943 0.55 +0.4 4,581,230 - Pf6N2E2_3943 0.55 -0.0 4,581,262 + Pf6N2E2_3943 0.59 -0.5 4,581,263 - Pf6N2E2_3943 0.59 +0.4 4,581,263 - Pf6N2E2_3943 0.59 -0.2 4,581,263 - Pf6N2E2_3943 0.59 +0.3 4,581,269 + Pf6N2E2_3943 0.60 -1.0 4,581,270 - Pf6N2E2_3943 0.60 +0.6 4,581,478 + Pf6N2E2_3943 0.86 +0.2 4,581,478 + Pf6N2E2_3943 0.86 -0.1 4,581,479 - Pf6N2E2_3943 0.87 -0.2 4,581,540 + -0.0 4,581,540 + -0.4 4,581,540 + -1.0 4,581,540 + +1.2 4,581,541 - -0.0 4,581,541 - +0.6 4,581,574 + +0.3 4,581,575 - +0.5 4,581,575 - +0.2 4,581,591 + +0.6 4,581,592 - +0.6 4,581,640 - +0.7 4,581,647 - +0.3 4,581,694 + +0.6 4,581,760 + -0.8 4,581,786 - +0.2 4,581,858 + +0.6 4,581,859 - +0.4 4,581,901 + -0.1 4,581,901 + +0.0 4,581,902 - -0.3 4,581,902 - -0.9 4,582,460 + Pf6N2E2_3944 0.74 -0.4 4,582,460 + Pf6N2E2_3944 0.74 +0.4 4,582,506 + Pf6N2E2_3944 0.80 +0.5 4,582,506 + Pf6N2E2_3944 0.80 +2.2 4,582,507 - Pf6N2E2_3944 0.80 +0.3 4,582,522 + Pf6N2E2_3944 0.82 -0.1 4,582,522 + Pf6N2E2_3944 0.82 -0.3 4,582,637 + -0.7 4,582,637 + -0.1 4,582,637 + -0.7 4,582,637 + +0.1 4,582,637 + -0.5 4,582,682 + +0.2 4,582,723 + +0.3 4,583,511 + -0.8 4,583,511 + +0.2
Or see this region's nucleotide sequence