Experiment: Spectinomycin 0.01 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf6N2E2_3644 and Pf6N2E2_3645 are separated by 435 nucleotides Pf6N2E2_3645 and Pf6N2E2_3646 are separated by 193 nucleotides 
        Pf6N2E2_3644 - Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17), at 4,243,510 to 4,245,399 
        _3644 
         
        
        Pf6N2E2_3645 - Type I secretion outer membrane protein, TolC precursor, at 4,245,835 to 4,247,274 
        _3645 
         
        
        Pf6N2E2_3646 - 3-deoxy-D-manno-octulosonic-acid transferase (EC 2.-.-.-), at 4,247,468 to 4,248,718 
        _3646 
         Position (kb) 4245 
4246 
4247 
4248 Strain fitness (log2 ratio) -2 
-1 
0 
1 
2 at 4244.930 kb on + strand, within Pf6N2E2_3644 at 4244.931 kb on - strand, within Pf6N2E2_3644 at 4244.931 kb on - strand, within Pf6N2E2_3644 at 4244.931 kb on - strand, within Pf6N2E2_3644 at 4244.931 kb on - strand, within Pf6N2E2_3644 at 4244.966 kb on + strand, within Pf6N2E2_3644 at 4244.966 kb on + strand, within Pf6N2E2_3644 at 4244.967 kb on - strand, within Pf6N2E2_3644 at 4245.200 kb on + strand, within Pf6N2E2_3644 at 4245.201 kb on - strand, within Pf6N2E2_3644 at 4245.656 kb on + strand at 4245.656 kb on + strand at 4245.656 kb on + strand at 4245.657 kb on - strand at 4245.657 kb on - strand at 4245.657 kb on - strand at 4245.746 kb on + strand at 4245.746 kb on + strand at 4245.746 kb on + strand at 4245.746 kb on + strand at 4245.746 kb on + strand at 4245.746 kb on + strand at 4245.746 kb on + strand at 4245.747 kb on - strand at 4245.747 kb on - strand at 4245.747 kb on - strand at 4245.748 kb on + strand at 4245.748 kb on + strand at 4245.748 kb on + strand at 4245.748 kb on + strand at 4245.748 kb on + strand at 4245.749 kb on - strand at 4245.749 kb on - strand at 4245.749 kb on - strand at 4245.749 kb on - strand at 4245.749 kb on - strand at 4245.778 kb on + strand at 4245.779 kb on - strand at 4245.779 kb on - strand at 4245.822 kb on + strand at 4245.822 kb on + strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.940 kb on + strand at 4245.940 kb on + strand at 4245.940 kb on + strand at 4246.126 kb on + strand, within Pf6N2E2_3645 at 4246.408 kb on + strand, within Pf6N2E2_3645 at 4246.409 kb on - strand, within Pf6N2E2_3645 at 4246.409 kb on - strand, within Pf6N2E2_3645 at 4246.495 kb on + strand, within Pf6N2E2_3645 at 4246.495 kb on + strand, within Pf6N2E2_3645 at 4246.496 kb on - strand, within Pf6N2E2_3645 at 4246.573 kb on - strand, within Pf6N2E2_3645 at 4246.599 kb on - strand, within Pf6N2E2_3645 at 4246.612 kb on + strand, within Pf6N2E2_3645 at 4246.613 kb on - strand, within Pf6N2E2_3645 at 4246.613 kb on - strand, within Pf6N2E2_3645 at 4246.635 kb on + strand, within Pf6N2E2_3645 at 4246.636 kb on - strand, within Pf6N2E2_3645 at 4246.691 kb on - strand, within Pf6N2E2_3645 at 4246.691 kb on - strand, within Pf6N2E2_3645 at 4246.794 kb on + strand, within Pf6N2E2_3645 at 4247.065 kb on + strand, within Pf6N2E2_3645 at 4247.065 kb on + strand, within Pf6N2E2_3645 at 4247.066 kb on - strand, within Pf6N2E2_3645 at 4247.066 kb on - strand, within Pf6N2E2_3645 at 4247.066 kb on - strand, within Pf6N2E2_3645 at 4247.066 kb on - strand, within Pf6N2E2_3645 at 4247.066 kb on - strand, within Pf6N2E2_3645 at 4247.083 kb on + strand, within Pf6N2E2_3645 at 4247.083 kb on + strand, within Pf6N2E2_3645 at 4247.083 kb on + strand, within Pf6N2E2_3645 at 4247.083 kb on + strand, within Pf6N2E2_3645 at 4247.084 kb on - strand, within Pf6N2E2_3645 at 4247.084 kb on - strand, within Pf6N2E2_3645 at 4247.084 kb on - strand, within Pf6N2E2_3645 at 4247.104 kb on + strand, within Pf6N2E2_3645 at 4247.104 kb on + strand, within Pf6N2E2_3645 at 4247.104 kb on + strand, within Pf6N2E2_3645 at 4247.104 kb on + strand, within Pf6N2E2_3645 at 4247.104 kb on + strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.179 kb on + strand at 4247.180 kb on - strand at 4247.194 kb on + strand at 4247.194 kb on + strand at 4247.195 kb on - strand at 4247.195 kb on - strand at 4247.195 kb on - strand at 4247.272 kb on + strand at 4247.273 kb on - strand at 4247.277 kb on + strand at 4247.277 kb on + strand at 4247.278 kb on - strand at 4247.278 kb on - strand at 4247.278 kb on - strand at 4247.278 kb on - strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.286 kb on - strand at 4247.286 kb on - strand at 4247.286 kb on - strand at 4247.286 kb on - strand at 4247.286 kb on - strand at 4247.286 kb on - strand at 4247.286 kb on - strand at 4247.300 kb on - strand at 4247.348 kb on + strand at 4247.348 kb on + strand at 4247.348 kb on + strand at 4247.411 kb on + strand at 4247.412 kb on - strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Spectinomycin 0.01 mg/ml remove 4,244,930 +  Pf6N2E2_3644 0.75  -0.7 4,244,931 -  Pf6N2E2_3644 0.75  -0.4 4,244,931 -  Pf6N2E2_3644 0.75  +1.4 4,244,931 -  Pf6N2E2_3644 0.75  -0.8 4,244,931 -  Pf6N2E2_3644 0.75  +0.6 4,244,966 +  Pf6N2E2_3644 0.77  -0.1 4,244,966 +  Pf6N2E2_3644 0.77  +0.1 4,244,967 -  Pf6N2E2_3644 0.77  +0.8 4,245,200 +  Pf6N2E2_3644 0.89  -0.2 4,245,201 -  Pf6N2E2_3644 0.89  -0.1 4,245,656 +  +1.0 4,245,656 +  +0.1 4,245,656 +  -0.4 4,245,657 -  +0.5 4,245,657 -  +0.4 4,245,657 -  +0.6 4,245,746 +  +0.7 4,245,746 +  -1.8 4,245,746 +  -0.3 4,245,746 +  +0.7 4,245,746 +  +0.4 4,245,746 +  +0.7 4,245,746 +  +0.1 4,245,747 -  -0.2 4,245,747 -  +0.5 4,245,747 -  +1.1 4,245,748 +  +0.2 4,245,748 +  +0.4 4,245,748 +  -0.0 4,245,748 +  +0.4 4,245,748 +  +0.2 4,245,749 -  -0.1 4,245,749 -  +0.5 4,245,749 -  -0.8 4,245,749 -  -0.0 4,245,749 -  -0.0 4,245,778 +  -1.0 4,245,779 -  -1.8 4,245,779 -  +0.6 4,245,822 +  +0.2 4,245,822 +  +1.3 4,245,823 -  -2.0 4,245,823 -  -0.5 4,245,823 -  -0.1 4,245,823 -  +0.3 4,245,823 -  +0.6 4,245,823 -  +0.4 4,245,823 -  +0.0 4,245,823 -  -0.1 4,245,940 +  -0.9 4,245,940 +  +1.0 4,245,940 +  +0.2 4,246,126 +  Pf6N2E2_3645 0.20  +0.3 4,246,408 +  Pf6N2E2_3645 0.40  +0.8 4,246,409 -  Pf6N2E2_3645 0.40  +0.2 4,246,409 -  Pf6N2E2_3645 0.40  +0.2 4,246,495 +  Pf6N2E2_3645 0.46  +0.3 4,246,495 +  Pf6N2E2_3645 0.46  -0.0 4,246,496 -  Pf6N2E2_3645 0.46  +1.9 4,246,573 -  Pf6N2E2_3645 0.51  +0.4 4,246,599 -  Pf6N2E2_3645 0.53  -0.8 4,246,612 +  Pf6N2E2_3645 0.54  +0.1 4,246,613 -  Pf6N2E2_3645 0.54  +0.6 4,246,613 -  Pf6N2E2_3645 0.54  -0.2 4,246,635 +  Pf6N2E2_3645 0.56  -0.9 4,246,636 -  Pf6N2E2_3645 0.56  +0.3 4,246,691 -  Pf6N2E2_3645 0.59  +1.1 4,246,691 -  Pf6N2E2_3645 0.59  +0.3 4,246,794 +  Pf6N2E2_3645 0.67  +0.5 4,247,065 +  Pf6N2E2_3645 0.85  +0.6 4,247,065 +  Pf6N2E2_3645 0.85  +0.4 4,247,066 -  Pf6N2E2_3645 0.85  -0.7 4,247,066 -  Pf6N2E2_3645 0.85  +0.6 4,247,066 -  Pf6N2E2_3645 0.85  +0.1 4,247,066 -  Pf6N2E2_3645 0.85  -0.3 4,247,066 -  Pf6N2E2_3645 0.85  -0.0 4,247,083 +  Pf6N2E2_3645 0.87  +0.6 4,247,083 +  Pf6N2E2_3645 0.87  -0.2 4,247,083 +  Pf6N2E2_3645 0.87  +0.2 4,247,083 +  Pf6N2E2_3645 0.87  +0.3 4,247,084 -  Pf6N2E2_3645 0.87  +0.0 4,247,084 -  Pf6N2E2_3645 0.87  +0.0 4,247,084 -  Pf6N2E2_3645 0.87  -0.2 4,247,104 +  Pf6N2E2_3645 0.88  +0.9 4,247,104 +  Pf6N2E2_3645 0.88  -0.1 4,247,104 +  Pf6N2E2_3645 0.88  +1.4 4,247,104 +  Pf6N2E2_3645 0.88  +1.3 4,247,104 +  Pf6N2E2_3645 0.88  +0.0 4,247,105 -  Pf6N2E2_3645 0.88  -0.2 4,247,105 -  Pf6N2E2_3645 0.88  +0.3 4,247,105 -  Pf6N2E2_3645 0.88  +0.7 4,247,105 -  Pf6N2E2_3645 0.88  +1.7 4,247,105 -  Pf6N2E2_3645 0.88  -0.1 4,247,105 -  Pf6N2E2_3645 0.88  +0.8 4,247,105 -  Pf6N2E2_3645 0.88  -0.4 4,247,179 +  +0.4 4,247,180 -  +2.2 4,247,194 +  +0.4 4,247,194 +  +0.4 4,247,195 -  -0.4 4,247,195 -  -0.7 4,247,195 -  +0.1 4,247,272 +  +0.4 4,247,273 -  +0.3 4,247,277 +  +2.3 4,247,277 +  +0.3 4,247,278 -  +0.3 4,247,278 -  -0.3 4,247,278 -  -0.0 4,247,278 -  -0.3 4,247,285 +  -0.2 4,247,285 +  -0.3 4,247,285 +  -0.6 4,247,285 +  +0.9 4,247,285 +  +0.5 4,247,285 +  -1.1 4,247,285 +  -0.1 4,247,285 +  +0.4 4,247,285 +  -0.6 4,247,286 -  +0.2 4,247,286 -  -0.6 4,247,286 -  -0.5 4,247,286 -  +0.4 4,247,286 -  +0.4 4,247,286 -  +0.0 4,247,286 -  +0.3 4,247,300 -  -0.2 4,247,348 +  -0.4 4,247,348 +  +1.2 4,247,348 +  +0.2 4,247,411 +  -0.8 4,247,412 -  -0.1 
Or see this region's nucleotide sequence